Mutagenic potential evaluation of the water of a river that receives tannery effluent using the Allium cepa test system

Several studies are being conducted to assess the toxicity and cytotoxicity of water bodies receiving industrial and domestic effluents, using the Allium cepa test. To assess the toxicity and mutagenicity of water possibly contaminated with chromium, derived from tannery activities, seasonal water samplings were performed in 2001 and 2002 at five different sites along the Sapucaizinho river, Municipality of Patrocinio Paulista, State of São Paulo, Brazil. A. cepa seeds were used as the test material and were submitted to germination in waters from the different collection sites, in Milli-Q water (negative control) and in aqueous solution of chromium (positive control). For the determination of cell division rates and mitotic irregularities, slides were prepared with root tip cells according to the standard Feulgen methodology. The results showed that the collection sites most heavily compromised by chromium emission presented low mitotic indices and a higher frequency of mitotic changes such as irregular anaphases (disorganized, multipolar, laggard), cells with chromosomal adherences, cells with micronuclei, and binucleate and/or multinucleate cells. © 2004 The Japan Mendel Society.

Evaluation of the genotoxic potential due to the action of an effluent contaminated with chromium, by the comet assay in CHO-K1 cultures

The comet assay technique has been considered to be more efficient in the biomonitoring of aquatic environments that the micronucleus and sister chromatid exchange techniques. The comet assay has been used to determine breaks in the DNA strands of organisms exposed to pollutants with a genotoxic potential. The comet technique was applied to CHO-K1 cells in order to evaluate the genotoxic potential of the waters of the Sapucaizinho River (Municipality of Patrocínio Paulista, State of São Paulo, Brazil), which receive tannery effluents and therefore are contaminated with chromium. The results indicated high genotoxicity of the waters collected at sites located downstream from the emission of tannery effluents, where the concentration of chromium was found to be high.

Anaerobic effluent disinfected with ozone/hydrogen peroxide efluente anaeróbio desinfetado com ozônio/peróxido de hidrogênio

This research aimed at studying the oxidation process, to verify the effectiveness of coliform inactivation and to evaluate the formation of ozonation disinfection byproducts (DBP) in anoxic sanitary wastewater treated with ozone/hydrogen peroxide applied at doses of 2.6 mg O3 L-1 and 2.0 mg H2O2 L-1 with contact time of 10 min and 8.1 mg O3 L-1 and 8.0 mg H2O2 L-1 with contact time of 20 min. The mean chemical oxygen demand (COD) reductions were 7.50 and 9.40% for applied dosages of 2.5-2.8 and 6.4-9.4 mg O3 L-1 + 2.0 and 8.0 mg H2O2.L-1, respectively. The Escherichia coli (E. coli) inactivation range was 2.98-4.04 log10 and the total coliform inactivation range was 2.77-4.01 log10. The aldehydes investigated were formaldehyde, acetaldehyde, glyoxal and methylglyoxal. It was observed only the formation of acetaldehyde that ranged 5.53 to 29.68 μg L-1.

Effects of intensification of the amazon river prawn, macrobrachium amazonicum, grow-out on effluent quality

Studies to determine suitable levels of intensification are essential for developing sustainable aquaculture. The objective of this study was to evaluate the quality of effluents discharged from ponds stocked with 10 (D10), 20 (D20), 40 (D40), and 80 (D80) postlarvae of Macrobrachium amazonicum/m2. Intake and effluent water samples were taken throughout a 5.5-mo grow-out cycle. In that study, twelve 0.01-ha earthen ponds were stocked postlarvae with 0.01g. Average water exchange rate was 15%/d; water was discharged from the bottom of the ponds. Prawns were fed a commercial feed with 38% crude protein according to their biomass (3-10%) and the concentration of dissolved oxygen (DO). In our research, temperature, turbidity, total suspended solids, conductivity, DO, pH, biochemical oxygen demand (BOD), chemical oxygen demand (COD), N-ammonia, N-nitrite, N-nitrate, N-Kjeldahl nitrogen, total phosphorus, and soluble orthophosphate were measured every 15d throughout the experiment in the early morning (0630 to 0730h). Turbidity was lower in D10 than in D20 and D40 and total phosphorus was higher in D80 than in D10 and D20. An analysis of principal components comparing treatments and intake water showed three groups: intake, D10 and a cluster of D20, D40, and D80. On the basis of the water characteristics found in our study it appears that the farming of M. amazonicum is likely to have a low environmental impact, at least up to a stocking density of 80prawns/m2. © by the World Aquaculture Society 2013.

Potentially pathogenic Escherichia coli in healthy, pasture-raised sheep on farms and at the abattoir in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Toxicity on aquatic organisms exposed to secondary effluent disinfected with chlorine, peracetic acid, ozone and UV radiation

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Genotoxicity and cytotoxicity induced by municipal effluent in multiple organs of Wistar rats

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Study of constructed wetlands effluent disinfected with ozone

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparing environmental impacts of native and introduced freshwater prawn farming in Brazil and the influence of better effluent management using LCA

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Constructed Wetland for Treating Effluent from Subtropical Aquaculture Farm

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Treatment efficiency of effluent prawn culture by wetland with floating aquatic macrophytes arranged in series

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

UASB reactor effluent disinfection by ozone and chlorine

This research studied the sequential ozone and chlorine process with respect to, the inactivation of indicator bacteria and the formation of ozone disinfection byproducts in sanitary wastewater effluent. The applied ozone doses were 5, 8 and 10 mg.O3.L(-1), followed by chlorine doses of 10, 20 and 30 mg.L(-1), respectively. After the sequential ozone/chlorine process, the mean reduction in chemical oxygen demand ranged from 9 to 37%. Total coliform inactivation ranged from 1.59 to 3.73 log10, and E. coli was always <1 CFU 100 mL(-1). Ozonation resulted in the formation of aldehydes, which were not significantly impacted by the subsequent chlorine dose (P ≤ 0.05).

Rapid determination of 12 antibiotics and caffeine in sewage and bioreactor effluent by online column-switching liquid chromatography/tandem mass spectrometry

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effluent volume and dialysis dose in CRRT: time for reappraisal

The results of several studies assessing dialysis dose have dampened the enthusiasm of clinicians for considering dialysis dose as a modifiable factor influencing outcomes in patients with acute kidney injury. Powerful evidence from two large, multicenter trials indicates that increasing the dialysis dose, measured as hourly effluent volume, has no benefit in continuous renal replacement therapy (CRRT). However, some important operational characteristics that affect delivered dose were not evaluated. Effluent volume does not correspond to the actual delivered dose, as a decline in filter efficacy reduces solute removal during therapy. We believe that providing accurate parameters of delivered dose could improve the delivery of a prescribed dose and refine the assessment of the effect of dose on outcomes in critically ill patients treated with CRRT.