High-resolution electrophoretic procedures for the identification of five Eimeria species from chickens, and detection of population variation

To overcome limitations of conventional approaches for the identification of Eimeria species of chickens, we have established high resolution electrophoretic procedures using genetic markers in ribosomal DNA. The first and second internal transcribed spacer (ITS-1 and ITS-2) regions of ribosomal DNA were amplified by polymerase chain reaction (PCR) from genomic DNA samples representing five species of Eimeria (E. acervulina, E. brunetti, E. maxima, E. necatrix and E. tenella), denatured and then subjected to denaturing polyacrylamide gel electrophoresis (D-PAGE) or single-strand conformation polymorphism (SSCP) analysis. Differences in D-PAGE profiles for both the ITS-1 and ITS-2 fragments (combined with an apparent lack of variation within individual species) enabled the unequivocal identification of the five species, and SSCP allowed the detection of population variation between some isolates representing E. acervulina, which remained undetected by D-PAGE. The establishment of these approaches has important implications for controlling the purity of laboratory lines of Eimeria, for diagnosis and for studying the epidemiology of coccidiosis.

DEUDA EXTERNA Y DEUDA PÚBLICA SUSTENTABLES EN ARGENTINA

Las propuestas de solución de un problema estructural tan grave como el de la deuda externa de los países del Sur en general y de la Argentina en particular no debería ser algo de exclusiva responsabilidad de los gobiernos.Esto significa que todos los estamentos de la sociedad civil deben asumir su responsabilidad en construir una opinión pública sobre qué hacer frente al problema de la deuda externa, porque será el conjunto social el que se beneficiará de una correcta solución de este problema o el que sufrirá las gravísimas consecuencias de cualquier variante que, directa o indirectamente, termine agravandoel subdesarrollo y la pobreza.

Diálogo con Win Pelupessy sobre cadenas agroalimentarias

Diálogo con Win Pelupessy sobre cadenas agroalimentarias

Nota de apresentação

Neste sétimo número da Revista Infância e Educação: Investigação e Práticas, coordenado por Ana Paula Cardoso e Esperança Jales Ribeiro, são essencialmente divulgados os trabalhos apresentados no IV Simpósio Internacional do GEDEI, Grupo de Estudos para o Desenvolvimento da Educação de Infância, que teve lugar na Aula Magna do Instituto Politécnico de Viseu.

Resolución 074 del 13 de junio de 2013: Por la cual se resuelve un trámite de revocatoria directa

Servicios registrales

Desafíos contemporáneos para el socialismo en Cuba (A propósito de las reformas políticoeconómicas en la fase actual de la revolución)

This essay analyzes on the origin and impact of the amendment process currently undertaken by the Cuban government authorities considering it as the result of --what I call-- super structural imbalance. Such imbalance is caused by the application of the System for Management and Planning of the Economy. To our judgment, the process of amendment both structurally and super structurally conforms the new phase in the construction of the Socialism in the island from a coherent point of view to what it has been until now as a political constant of the revolution: the vindication of the human being as the promoter of its historical reality.