Acanthoscurrin Fragment 101-132: Total Synthesis at 60 degrees C of a Novel Difficult Sequence

Glycine-rich proteins (GRP), serve a variety of biological functions. Acanthoscurrin is an antimicrobial GRP isolated front hemocytes-of the Brazilian spider Acanthoscurria gomesiana. Aiming to contribute to the knowledge of the secondary structure and stepwise solid-phase synthesis of GRPs` glycine-rich domains, we attempted to prepare G(101)GGLGGGRGGGYG(113) GGGGYGGGYG(123)GGy(126)GGGKYK(132)-NH(2), acanthoscurrin C-terminal amidated fragment. Although a theoretical prediction did not indicate high aggregation potential for this peptide, repetitive incomplete aminoacylations were observed after incorporating Tyr(126) to the growing peptide-MBHA resin (Boc chemistry) at 60 degrees C. The problem was not solved by varying the coupling reagents or solvents, adding chaotropic salts to the reaction media or changing the resin/chemistry (Rink amide resin/Fmoc chemistry). Some improvement was mode when CLEAR amide resin (Fmoc chemistry) was 32 used, as it allowed for obtaining fragment (G(113)-K(132) NIR-FT-Raman spectra collected for samples of the growing peptide-MBHA, -Rink amide resin and -CLEAR amide resin revealed the presence of beta-sheet structures. Only the combination of CLEAR-amide resin, 60 degrees C, Fmoc-(Fmoc-Hmb)Gly-OH and LiCl (the last two used alternately) was able to inhibit the phenomenon, as proven by NIR-FT-Raman analysis of the growing peptide-resin, allowing the total synthesis of desired 132 fragment Gly(101)-K(132). In summary, this work describes a new difficult sequence, contributes to understanding stepwise solid-phase synthesis of this type of peptide and shows that, at least while protected and linked to a resin, this GRPs glycine-rich motif presents all early tendency to assume beta-sheet structures. (c) 2008 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 92: 65-75, 2009.

Praia do Flamengo, 132: memória, reparação e patrimonialização da União Nacional dos Estudantes

O presente trabalho visa analisar o processo de patrimonialização da União Nacional dos Estudantes, promovido na articulação de memória e reparação, mobilizando elementos do passado da instituição, especialmente os eventos ocorridos no antigo prédio de sua sede, no presente momento conhecido como “terreno da UNE”, na Praia do Flamengo, 132. Tem como objetivo entender como os militantes, no quadro geral de busca por reverter um processo de esvaziamento de capital político da instituição, promovem a reconversão de seu capital simbólico, mais amplo, em capital político, mais específico, potencializando-a novamente no campo político. Decorrente disso se identifica um processo a que foi nomeado patrimonialização da UNE, ou seja, a ação de reconhecê-la como elemento pertencente à retórica do patrimônio cultural brasileiro. Buscou-se adotar uma abordagem diacrônica e sincrônica. No primeiro momento, foi preciso dar conta das ações da entidade estudantil, que tiveram lugar no "prédio da UNE", ao longo do tempo para, posteriormente, perscrutar as maneiras como estes atos do passado foram recuperados e instrumentalizados no jogo político do presente. Foram analisados, primeiramente, os atos legais que envolveram o prédio/terreno, a fim de reconstruir uma história para a atuação da UNE naquele espaço. A partir desta se procurou entender os usos deste passado pelos agentes do presente, promovidos por meio da realização de projetos culturais e do processo de elaboração e instituição da Lei 12.260/2010, que reconhece a responsabilidade do Estado pelos atos ocorridos no prédio da Praia do Flamengo durante o regime militar e indeniza a instituição como forma de reparação. Assim, é na imbricação dos campos da política e da cultura que se desenvolve o processo que neste trabalho se procurou investigar.

Clinical evaluation of the bicoronal flap in the treatment of facial fractures. Retrospective study of 132 patients

The aim of the present study was to evaluate the postoperative complications of bicoronal flaps used to treat facial fractures. One hundred and thirty two patients that received bicoronal flaps for the treatment of upper and middle third facial fractures were called for clinical and radiographic examination. Minimum follow-up was 1 year and all patients had charts with adequate information about their perioperative care pertinent to the study. Results showed as complications hypoesthesia (17%), partial unilateral frontal motor deficit (11%), infection (3%), hypertrophic scars (3%), varying degrees of alopecia (18%), seroma or hematoma in the immediate postoperative period (5%). The flap provided wide surgical access to the upper and middle facial thirds with very few serious complications, most frequently allowing good aesthetic results. (C) 2011 European Association for Cranio-Maxillo-Facial Surgery.

DOCPAL resúmenes sobre población en América Latina: 132-001

Contiene resúmenes bibliográficos de documentos sobre población y temas relacionados que se producen en América Latina y el Caribe, publicados entre 1986-1989.

Desempenho operacional e energético, segundo a norma OECD – code 2 de dois tratores agrícolas 4x2 TDA com motores de 132 kw em pista concreto e solo agrícola

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Clinical evaluation of the bicoronal flap in the treatament of facial fractures: retrospective study of 132 patients

The aim of the present study was to evaluate the postoperative complications of bicoronal flaps used to treat facial fractures. One hundred and thirty two patients that received bicoronal flaps for the treatment of upper and middle third facial fractures were called for clinical and radiographic examination. Minimum follow-up was 1 year and all patients had charts with adequate information about their perioperative care pertinent to the study. Results showed as complications hypoesthesia (17%), partial unilateral frontal motor deficit (11%), infection (3%), hypertrophic scars (3%), varying degrees of alopecia (18%), seroma or hematoma in the immediate postoperative period (5%). The flap provided wide surgical access to the upper and middle facial thirds with very few serious complications, most frequently allowing good aesthetic results.