Oral glands in dipsadine ""goo-eater"" snakes: Morphology and histochemistry of the infralabial glands in Atractus reticulatus, Dipsas indica, and Sibynomorphus mikanii

Although snake infralabial glands are generally constituted of mucous cells, among dipsadines, they are much more developed and predominantly serous in nature, possibly due to the peculiar feeding habits of some species of this group, the ""goo-eaters"", which feed on soft and viscous invertebrates. We compared the morphology and histochemistry of the infralabial glands of three goo-eater species of Southeast Brazil, Atractus reticulatus, Dipsas indica and Sibynomorphus mikanii. In A. reticulatus the glands are formed by mixed acini composed of mucous and seromucous cells and in D. indica, they are composed of mucous tubules and seromucous acini. In S. mikanii the glands are organized in seromucous acini; mucous cells are restricted to the gland anterior region and to the duct lining epithelium. Ultrastructurally, secretory granule electron density varies from low to moderate, depending on their mucous or seromucous nature. The results indicate a large morphological and histochemical variation in the infralabial glands, probably reflecting differences in the secretion chemical composition and in feeding specialization among the three species. The protein content in the secretory cells can be related with the presence of toxins that can be used in chemical prey immobilization or detaching of snails from their shells. (c) 2007 Elsevier Ltd. All rights reserved.

LIZARDS AND SNAKES (LEPIDOSAURIA, SQUAMATA) FROM THE LATE QUATERNARY OF THE STATE OF CEARA IN NORTHEASTERN BRAZIL

We present the first formal report on the squamate assemblage from Parque Nacional de Ubajara. This park contains the most important cave complex in the state of Ceara in northeastern Brazil, called Provincia Espeleologica de Ubajara. The material comes from the Urso Fossil cave at Pendurado Hill. All previously reported fossil remains found in this cave are tentatively attributed to the Quaternary (late Pleistocene-early Holocene). Probably only Arctotherium brasiliense represents a relictual fossil bear from the late Pleistocene megafauna. The taxa recognized in this paper belong to Tropidurus sp., Ameiva sp., cf. Epicrates, and cf. Crotalus durissus, adding to the knowledge of the Brazilian Quaternary squamate fauna as a whole, and contribute to a major taxonomic refinement of the squamate assemblages from the early Holocene of northeastern Brazil.

Circadian pattern of Bothrops moojeni in captivity (Serpentes: Viperidae)

Members of the subfamily Crotalinae are considered to be essentially nocturnal and most of the data about these snakes have been collected from the field. Information on how nutritional status affects the movement rate and activity patterns is a key point to elucidating the ecophysiology of snakes. In this study, we distributed 28 lancehead Bothrops moojeni into three groups under distinct feeding regimens after a month of fasting. Groups were divided as follows: ingestion of meals weighing (A) 40%, (B) 20%, or (C) 10% of the snake body mass. Groups were monitored for five days before and after food intake and the activity periods and movement rates were recorded. Our results show that B. moojeni is prevalently nocturnal, and the activity peak occurs in the first three hours of the scotophase. After feeding, a significant decrease in activity levels in groups A and B was detected. The current results corroborate previous field data that describe B. moojeni as a nocturnal species with low movement rates. The relationship between motion and the amount of food consumed by the snake may be associated with its hunting strategy.

The Evolution of Diet and Microhabitat Use in Pseudoboine Snakes

Reflecting their exceptional radiation, snakes occur in different habitats and microhabitats and are able to eat numerous types of prey. The availability of good and comprehensive phylogenies for different snake’s lineages together with natural history data provides an opportunity to explore how ecological traits diversified during their radiation. In the present study, we describe the diet and microhabitat variation (arboreal or non-arboreal) in the tribe Pseudoboini and explore how these traits evolved during the tribe’s diversification. We analyzed specimens deposited in scientific collections and gathered information on diet and microhabitat use available in the literature and provided by other researchers. We also mapped diet and microhabitat data onto a phylogeny of the tribe using the principle of parsimony. Pseudoboine snakes feed mainly on lizards and small mammals, and of the 22 species for which a minimum number of prey records was obtained, nine are diet generalists, six are lizard specialists, three are small mammal specialists, two are snake specialists, one is a lizard egg specialist, and one is a bird egg specialist. The highly diverse feeding habits of pseudoboines seem to have evolved mainly in the terminal taxa. Among those species that had enough microhabitat data (17 species), Drepanoides anomalus, Siphlophis cervinus, S. compressus, and S. pulcher frequently use the vegetation. Our results indicate that an increase in arboreality evolved several times during the diversification of the tribe, and that the Siphlophis clade seems to have maintained the high degree of arboreality from its ancestor. Species that frequently use vegetation are either lizard or lizard egg specialists, indicating that these habits might be associated in the evolution of pseudoboines.

Proteomic characterisation of toxins isolated from nematocysts of the South Atlantic jellyfish Olindias sambaquiensis

Surprisingly little is known of the toxic arsenal of cnidarian nematocysts compared to other venomous animals. Here we investigate the toxins of nematocysts isolated from the jellyfish Olindias sambaquiensis. A total of 29 unique ms/ms events were annotated as potential toxins homologous to the toxic proteins from diverse animal phyla, including conesnails, snakes, spiders, scorpions, wasp, bee, parasitic worm and other Cnidaria. Biological activities of these potential toxins include cytolysins, neurotoxins, phospholipases and toxic peptidases. The presence of several toxic enzymes is intriguing, such as sphingomyelin phosphodiesterase B (SMase B) that has only been described in certain spider venoms, and a prepro-haystatin P-IIId snake venom metalloproteinase (SVMP) that activates coagulation factor X, which is very rare even in snake venoms. Our annotation reveals sequence orthologs to many representatives of the most important superfamilies of peptide venoms suggesting that their origins in higher organisms arise from deep eumetazoan innovations. Accordingly, cnidarian venoms may possess unique biological properties that might generate new leads in the discovery of novel pharmacologically active drugs.

Detection of Chlamydia pneumoniae in a collection of captive snakes and response to treatment with marbofloxacin.

In a collection of 58 snakes comprising predominantly Eurasian vipers in Switzerland, five snakes died unexpectedly during hibernation from 2009 to 2012. In one snake, organisms resembling chlamydiae were detected by immunohistochemistry in multiple histiocytic granulomas. Real-time quantitative PCR and microarray analysis were used to determine the presence of Chlamydia pneumoniae in tissue samples and cloacal/choanal swabs from snakes in the collection; 8/53 (15.1%) of the remaining snakes were positive. Although one infected snake had suppurative periglossitis, infection with C. pneumoniae did not appear to be associated with specific clinical signs in snakes. Of seven snakes treated with 5 mg/kg marbofloxacin IM once daily, five became PCR negative for C. pneumoniae following treatment, whereas one animal remained positive and one snake was lost to follow-up.

Biogeographic regionalization of snakes in the Atlantic Forest hotspot: interpolating the faunal dissimilarity

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Segmentación de la glotis en imágenes laríngeas usando snakes.

El presente trabajo describe una nueva metodología para la detección automática del espacio glotal de imágenes laríngeas tomadas a partir de 15 vídeos grabados por el servicio ORL del hospital Gregorio Marañón de Madrid con luz estroboscópica. El sistema desarrollado está basado en el modelo de contornos activos (snake). El algoritmo combina en el pre-procesado, algunas técnicas tradicionales (umbralización y filtro de mediana) con técnicas más sofisticadas tales como filtrado anisotrópico. De esta forma, se obtiene una imagen apropiada para el uso de las snakes. El valor escogido para el umbral es del 85% del pico máximo del histograma de la imagen; sobre este valor la información de los píxeles no es relevante. El filtro anisotrópico permite distinguir dos niveles de intensidad, uno es el fondo y el otro es la glotis. La inicialización se basa en obtener el módulo del campo GVF; de esta manera se asegura un proceso automático para la selección del contorno inicial. El rendimiento del algoritmo se valida usando los coeficientes de Pratt y se compara contra una segmentación realizada manualmente y otro método automático basado en la transformada de watershed. SUMMARY: The present work describes a new methodology for the automatic detection of the glottal space from laryngeal images taken from 15 videos recorded by the ENT service of the Gregorio Marañon Hospital in Madrid with videostroboscopic equipment. The system is based on active contour models (snakes). The algorithm combines for the pre-processing, some traditional techniques (thresholding and median filter) with more sophisticated techniques such as anisotropic filtering. In this way, we obtain an appropriate image for the use of snake. The value selected for the threshold is 85% of the maximum peak of the image histogram; over this point the information of the pixels is not relevant. The anisotropic filter permits to distinguish two intensity levels, one is the background and the other one is the glottis. The initialization is based on the obtained magnitude by GVF field; in this manner an automatic process for the initial contour selection will be assured. The performance of the algorithm is tested using the Pratt coefficient and compared against a manual segmentation and another automatic method based on the watershed transformation.

A new approach for the glotis segmentation using Snakes

The present work describes a new methodology for the automatic detection of the glottal space from laryngeal images based on active contour models (snakes). In order to obtain an appropriate image for the use of snakes based techniques, the proposed algorithm combines a pre-processing stage including some traditional techniques (thresholding and median filter) with more sophisticated ones such as anisotropic filtering. The value selected for the thresholding was fixed to the 85% of the maximum peak of the image histogram, and the anisotropic filter permits to distinguish two intensity levels, one corresponding to the background and the other one to the foreground (glottis). The initialization carried out is based on the magnitude obtained using the Gradient Vector Flow field, ensuring an automatic process for the selection of the initial contour. The performance of the algorithm is tested using the Pratt coefficient and compared against a manual segmentation. The results obtained suggest that this method provided results comparable with other techniques such as the proposed in (Osma-Ruiz et al., 2008).

Detección automática con snakes y representación 3D sobre imágenes cerebrales

Comunicación presentada en la VI Conferencia de la Asociación Española para la Inteligencia Artificial (CAEPIA'95), Alicante, 15-17 noviembre 1995.

Snakes based tracking and texture analysis of microscopic images

Comunicación presentada en el VII Symposium Nacional de Reconocimiento de Formas y Análisis de Imágenes, SNRFAI, Barcelona, abril 1997.