Robert Holcot, Postilla super librum Sapientiae.

Ecrit par le copiste Bertrand (f. 170v).

Epidermal growth factor and bovine growth hormone stimulate differentiation and myelination of brain cell aggregates in culture.

Bovine growth hormone (bGH) and epidermal growth factor (EGF) increased the activity of ornithine decarboxylase (ODC) in brain cell aggregates cultured in a serum-free chemically defined medium. ODC is considered as a marker of cell growth and differentiation. The effect of bGH and EGF on myelination was investigated by measuring two myelin markers, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and myelin basic protein (MBP). EGF treatment at days 2 and 5 caused a dose-dependent increase of both myelin markers at culture day 12. This increase could still be observed at culture day 19, indicating a prolonged action of EGF. The continual presence of bGH in the culture medium produced a large accumulation of MBP at day 19. This effect was dose-dependent and required the presence of triiodothyronine (T3). In contrast, the effect of bGH on CNP activity did not require the presence of T3. This is the first report showing a direct effect of bGH on CNS myelination in vitro and of EGF on both MBP accumulation and ODC activity.

Commentarii super Aristotelem [dont: Isagoge (1-16v), Categoriae decem (19bis-54v)].

Ex-libris "S. Cornelii Compendiensis Congr. S. Mauri", suivi du chiffre 91B, XVIIe s., f. 1. Compiègne.

Fulgentius, Mythologie, phisica expositio super XII libros eneidorum (16), expositio sermonum ad Calcidium (20 v°). - Pauli et Senece epistole (22 v°). - Xenia M. Valerii Martialis (23). - Honorius, philosophia mundi (26 v°). - Prologus quorumdam juridicorum tractatuum (54 v°). - Ex Ausonio (55). -

Cordeliers.

Expositio Alani de Rupe super regulam b. Augustini.

Jacobins de la rue Saint-Honoré.

PEG-b-PPS diblock copolymer aggregates for hydrophobic drug solubilization and release: cyclosporin A as an example

Micelles formed from amphiphilic block copolymers have been explored in recent years as carriers for hydrophobic drugs. In an aqueous environment, the hydrophobic blocks form the core of the micelle, which can host lipophilic drugs, while the hydrophilic blocks form the corona or outer shell and stabilize the interface between the hydrophobic core and the external medium. In the present work, mesophase behavior and drug encapsulation were explored in the AB block copolymeric amphiphile composed of poly(ethylene glycol) (PEG) as a hydrophile and poly(propylene sulfide) PPS as a hydrophobe, using the immunosuppressive drug cyclosporin A (CsA) as an example of a highly hydrophobic drug. Block copolymers with a degree of polymerization of 44 on the PEG and of 10, 20 and 40 on the PPS respectively (abbreviated as PEG44-b-PPS10, PEG44-b-PPS20, PEG44-b-PPS40) were synthesized and characterized. Drug-loaded polymeric micelles were obtained by the cosolvent displacement method as well as the remarkably simple method of dispersing the warm polymer melt, with drug dissolved therein, in warm water. Effective drug solubility up to 2 mg/mL in aqueous media was facilitated by the PEG- b-PPS micelles, with loading levels up to 19% w/w being achieved. Release was burst-free and sustained over periods of 9-12 days. These micelles demonstrate interesting solubilization characteristics, due to the low glass transition temperature, highly hydrophobic nature, and good solvent properties of the PPS block

Postilla super epistolas et evangelia : tam de tempore quam de sancti : per totum anni circulum ([Reprod.]) / Hugonis de Sancto Charo

Collection : French books before 1601 ; 66.1

Michaelis Scoti super auctorem spherae cum quaestionibus... ([Reprod.])

Collection : Italian books before 1601 ; 59.5

Dialogua familiaris Alani et sodalis super deploratione calamitatis gallicane.

Franc. Philelfi orationes (34). - Philelfus Francisco Sforcie (92). - Guarinus Veron. Conrado Alcaldo (96). - De matrimonio inter Stephanum Salvanum et Margaritam Pontam (96 v°). - Plutarchus, de liberis educandis, interprete Garino Veron. (101).

Mercado Calú & Ângela e Palácio Finícia Controlo Interno como suporte estratégico de Gestão nas Empresas: os casos dos Super Mercado Calú & Ângela e Palácio Finícia

O presente trabalho intitula-se «Controlo Interno como suporte estratégico na Gestão das Empresas», pressupõe que todas as instituições devem ter um departamento de controlo interno para que possam desempenhar as suas actividades com eficiência e eficácia para alcançarem os objectivos estabelecidos pela administração. Porem, este trabalho teve como o principal objectivo definir e ampliar uma visão da importância do controlo interno nas instituições, pondo o foco no estudo de caso do Grupo Calú & Ângela, e Palácio Finícia. O controlo interno define-se como um conjunto de normas, procedimentos, instrumentos e acções utilizados pelas instituições ou empresas de uma forma sistematizada com o objectivo de assegurar o comprimento das normas e regulamentos internos.Partindo do pressuposto que, o controlo interno é uma ferramenta essencial para a gestão das instituições que prima pela salvaguarda dos seus activos e de prever eventuais riscos que possa pôr em causa a credibilidade das instituições, como, fraude, receptação de mercadorias diteoradas e fora do prazo, essas empresas devem ter um sistema de controlo interno robusta capaz de detectar essas situações constrangedoras. Portanto, os objectivos traçados e alcançados no trabalho é de uma interacção com o quotidiano das empresas comerciais em cabo verde, uma aproximação da teoria aprendida, e a prática desenvolvida durante o estágio curricular que teve uma metodologia virada pela pesquisas bibliográficas, pesquisas na internet, e aplicação do questionário.

HIV-1 co/super-infection in intravenous drug users.

BACKGROUND: The frequency of HIV-1 co/super-infection is unknown despite their implications for public health and vaccine development. This issue was addressed during an epidemic of both CRF11 and B subtype among intravenous drug users (IVDUs). METHODS: Bulk sequencing of reverse transcriptase, protease and C2V3 regions and subtype-specific nested polymerase chain reaction (PCR) in plasma and proviral DNA were performed using baseline and follow-up samples collected in recently infected IVDUs between 1998-2002 and in IVDUs with chronic infection living in the same area and presenting an unexpected rise of viremia (> 1 log10). RESULTS: In 58 recently infected patients, three B/CRF-11 co-infections, 25 B, 28 CRF-11 and two other subtypes were detected at baseline. In the three co-infected patients, both CRF-11 and B were detected in plasma and proviral DNA and persisted during follow-up. B- and CFR-11-specific PCR performed on follow-up samples of 40 of 58 recently infected patients (median follow-up, 14.5 months) revealed a transient B super-infection in a patient initially infected by CRF-11. Five of 156 chronic IVDUs (total follow-up: 346 years) had an unexpected rise of viremia. In two of them, aviremic without treatment for years after an initial B infection, a symptomatic CRF-11 super-infection occurred and was associated with high viral load and a fall of CD4 cell count. CONCLUSIONS: In recently infected IVDUs, co-infection B/CRF-11 is relatively frequent (5%). In chronically infected IVDUs super-infection may be transient and may occur in patients controlling efficiently HIV infection by the initial strain.

Active solubilization and refolding of stable protein aggregates by cooperative unfolding action of individual hsp70 chaperones.

Hsp70 is a central molecular chaperone that passively prevents protein aggregation and uses the energy of ATP hydrolysis to solubilize, translocate, and mediate the proper refolding of proteins in the cell. Yet, the molecular mechanism by which the active Hsp70 chaperone functions are achieved remains unclear. Here, we show that the bacterial Hsp70 (DnaK) can actively unfold misfolded structures in aggregated polypeptides, leading to gradual disaggregation. We found that the specific unfolding and disaggregation activities of individual DnaK molecules were optimal for large aggregates but dramatically decreased for small aggregates. The active unfolding of the smallest aggregates, leading to proper global refolding, required the cooperative action of several DnaK molecules per misfolded polypeptide. This finding suggests that the unique ATP-fueled locking/unlocking mechanism of the Hsp70 chaperones can recruit random chaperone motions to locally unfold misfolded structures and gradually disentangle stable aggregates into refoldable proteins.