Relevance of lovebirds (Agapornis roseicollis Selby, 1836) in experimental epidemiology of Newcastle disease

Studies were made to clarify the role that was played by the lovebirds (Agapornis roseicollis) in the epidemiological plan, under the perspective of its being a potential source of infection of Newcastle Disease Virus (NDV). The study used Specific-Pathogen-Free chicks (SPF) that were housed with lovebirds inoculated with a pathogenic strain (velogenic viscerotropic) of NDV pathogenic to chickens, by the ocular-nasal via. Each group was composed of six SPF chicks and four lovebirds. After five days of the inoculation of the lovebirds with NDV, SPF chicks were put together with each group of lovebirds. Cloacae swabs were collected after 9, 14 and 21 days post-challenge in both species (lovebirds and SPF chicks) for genome viral excretion by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Lovebirds did not demonstrate any clinical signs of NDV. They were refractory to the clinical disease with the NDV. However, NDV genome was detected 9 and 21 days after challenge. This study shows that lovebirds can be carriers NDV. Moreover, 100% of SPF chicks allocated with the infected lovebirds demonstrated clinical signs and lesions suggestive of NDV. In these birds, NDV genome was detected 9, 14 and 21 days after challenge. Thus, the transmission of the pathogenic virus from the lovebirds to SPF chicks that were housed together was evident until 21 days of the experimental infection. This study reveals the importance of lovebirds from the epidemiological point of view as potential source of infection of the NDV to other avian species that could be raised near this species. © Asian Network for Scientific Information, 2012.

Recent mouse and rat methods for the study of experimental oral candidiasis

The Candida genus expresses virulence factors that, when combined with immunosuppression and other risk factors, can cause different manifestations of oral candidiasis. The treatment of mucosal infections caused by Candida and the elucidation of the disease process have proven challenging. Therefore, the study of experimentally induced oral candidiasis in rats and mice is useful to clarify the etiopathology of this condition, improve diagnosis, and search for new therapeutic options because the disease process in these animals is similar to that of human candidiasis lesions. Here, we describe and discuss new studies involving rat and mouse models of oral candidiasis with respect to methods for inducing experimental infection, methods for evaluating the development of experimental candidiasis, and new treatment strategies for oral candidiasis. © 2013 Landes Bioscience.

Influence of experimental canine ehrlichiosis on the E-ADA activity and purine levels in serum and possible functional correlations with pathogenesis

The aim of this study was to evaluate adenosine deaminase activity and purines levels in serum of dogs experimentally infected by Ehrlichia canis. Banked serum samples of dogs divided into two groups with five animals each: healthy animals and animals infected by E. canis. The concentration of purines (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine, inosine, hypoxanthine, xanthine and uric acid), and adenosine deaminase (E-ADA) activity in sera were evaluated. Samples were collected on days 12 and 30 post-infection (PI). The E-ADA activity showed a significant reduction on day 12 PI, and increased on day 30 PI in dogs infected with E. canis. On day 12, an increase in seric concentration of ATP, ADP and adenosine was verified, and different levels of hypoxanthine, xanthine and uric acid had a drastic reduction in infected compared healthy dogs (P< 0.05). However, on day 30 PI, the levels of seric ADP and AMP decreased, unlike the concentration of xanthine and uric acid that increased significantly in infected dogs (P< 0.05). Therefore, the activity of E-ADA and purine levels are altered in experimental canine ehrlichiosis, probably with the purpose of modulating the pathogenesis of the disease related to immune response, oxidative stress and coagulation disorders in acute phase. © 2013 Elsevier B.V.

Ecotoxicidade e eficácia da oxitetraciclina e do florfenicol contra infecção experimental por Aeromonas hydrophila e aspectos histopatológicos em pacu (Piaractus mesopotamicus)

Pós-graduação em Aquicultura - FCAV

Infecção experimental em carcarás (Caracara plancus, Miller, J. F., 1777) com Toxoplasma gondii (amostra ME49)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Infecção experimental por Salmonella enterica subspécie enterica sorotipo Panama e tentativa de transmissão naso-nasal em leitões desmamados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Concentrações séricas de proteínas de fase aguda e IgG na infecção experimental por Ehrlichia canis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo clínico, laboratorial e terapêutico da diarréia experimental em bezerros induzida por Salmonella enterica subespécie Enterica sorotipo typhimurium

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Infecção experimental por Salmonella enterica subspécie enterica sorotipo Panama e tentativa de transmissão área em leitões desmamados

Pós-graduação em Medicina Veterinária - FCAV

Infecção experimental por Salmonella enterica subsp enterica sorovar Kottbus em pintos de corte de um dia e em ovos férteis SPF

Pós-graduação em Medicina Veterinária - FCAV

Infecção experimental com Salmonella Dublin em bezerros bubalinos: estudo clínico, laboratorial e terapêutico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo clínico e imunopatológico da infecção experimental em cães com a amostra Jaboticabal de Ehrlichia canis na fase aguda e após o tratamento: expreção de citocinas no baço e sangue e de subpopulações de células imunes no baço

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo clínico, laboratorial e terapêutico da diarréia experimental em bezerros induzida por Salmonella enterica subespécie enterica sorotipo Dublin

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Modelo experimental com caprinos e cobaias para avaliação da eficácia de vacinas contra o herpesvírus bovino tipo 1 e o vírus da diarreia viral bovina tipos 1 e 2

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Infecção experimental de Mazana gouazoubira (Ficher, 1814) (Cervidae: Odocoileinae) com Haemonchus contortus (Rudolphi, 1803) (Nematoda: Trichostrongyloidea)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)