Ciclo ovogenético e estudo morfológico comparativo do sistema reprodutivo de espécies da ordem Polydesmida (Diplopoda)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da incorporação do glicerol sobre a viabilidade e fertilidade do sêmen bovino refrigerado

Pós-graduação em Medicina Veterinária - FMVZ

Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski's horse (Equus ferus przewalskii)

Two studies were conducted to understand sperm cryosensitivity in an endangered equid, the Przewalski's horse (Equus ferns przewalski), while testing the cryoprotectant ability of formamides. The first assessed the toxicity of permeating cryoprotectants (glycerol, methylformamide IMF] and dimethylformamide [DMF]) to Przewalski's horse spermatozoa during liquid storage at 4 C. The second examined the comparative influence of three diluents (with or without formamides) on cryosurvival of sperm from the Przewalski's versus domestic horse. When Przewalski's horse spermatozoa were incubated at 4 C in INRA 96 with differing concentrations of glycerol, MF or DMF or a combination of these amides, cells tolerated all but the highest concentration (10% v/v) of MF alone or in combination with DMF, both of which decreased (P < 0.05) motility traits. There was no effect of cryoprotectants on sperm acrosomal integrity. In the cryosurvival study, average sperm motility and proportion of cells with intact acrosomes in fresh ejaculates were similar (P> 0.05) between the Przewalski's (67%, 84%, respectively) and domestic (66%, 76%) horse donors. Sperm from both species were diluted in lactose-EDTA-glycerol (EQ), Botu-Crio (BOTU; a proprietary product containing glycerol and MF) or SM (INRA 96 plus 2% [v/v] egg yolk and 2.5% [v/v] MF and DMF) and then frozen over liquid nitrogen vapor. After thawing, the highest values recovered for total and progressive sperm motility, acrosomal integrity and mitochondria] membrane potential were 42.4%, 21.8%, 88.7% and 25.4 CN (CN = mean JC-1 fluorescence intensity/cell on a channel number scale), respectively, in the Przewalski's and 49.3%, 24.6%, 88.9% and 25.8 CN, respectively, in the domestic horse. Although sperm progressive motility and acrosome integrity did not differ (P> 0.05) among treatments across species, mitochondrial membrane potential was higher (P< 0.05) in both species using EQ compared to BOTU or SM media. Additionally, Przewalski's stallion sperm expressed higher (P < 0.05) post-thaw total motility in BOTU and SM compared to EQ whereas there were no differences among freezing diluents in the domestic horse. In summary, Przewalski's stallion sperm benefit from exposure to either MF or DMF as an alternative cryoprotectant to glycerol. Overt sperm quality appears similar between the Przewalski's and domestic horse, although the total motility of cells from the former appears more sensitive to certain freezing diluents. Nonetheless, post-thaw motility and acrosomal integrity values for Przewalski's horse spermatozoa mimic findings in the domestic horse in the presence of INRA 96 supplemented with 2% (v/v) egg yolk and a combined 2.5% concentration of MF and DMF. Published by Elsevier Inc.

Propriedades estruturais e espectroscópicas de complexos de difenilfosfinatos dos sistemas ternários 'LA'-'CE'-'EU'/'LA'-CE'-'TB'

Pós-graduação em Química - IQ

Transporte placentário via cavéola na placenta de bovinos clonados e transgênicos

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Estudo termoanalítico de complexos do fármaco Ibuprofeno com lantanídeos (III) leves no estado sólido com exceção do promécio

This Final Paper had as it main goal to make a thermoanalytical study of lighter trivalent lanthanides (Lanthanum, Cerium, Praseodymium, Neodymium, Samarium and Europium) with the Ibuprofen ligand (nonsteroidal anti-inflammatory) that have a general formula LnL3.nH2O, on solid state, where Ln are the Lanthanides, L is the Ibuprofen ligand and n = number of water molecules of hydration that went from 1,0 to all the compounds. In order to characterize this compounds, it has been used the thermoanalytical techniques TG-DTA (thermogravimetry - Diferential Thermal Analysis) and DSC (Diferential Scanning Calorimetry), Fourier transformed infrared spectroscopy (FTIR) and complexometric titration with EDTA. Through the TG-DTA technique, it has been possible to set the thermal stability of the compounds, the number of thermal decomposition steps and temperatures that ocurred that also provided stoichiometry to the synthesized compounds. The DSC technique has shown the enthalpy of dehydration of the samarium and europium compounds, it was not possible to see it in the other compounds due to a endothermic peak on the DSC curve not being formed. In the case of neodymium, a thermal event ocurred, in which it could be a oxidative decarboxylation right after the dehydration. The infrared was utilised to study the carboxilate groups streches, and so, suggest a ligand metals compound coordination, that to this present paper has been a bidentade bridged coordenation. At last, the complexometric tritation was used to very the ammount of metal present in each compound, and so, verify if the proposed stoichiometry was according to the theory

Estudo do efeito do modificador Al-Sr no tratamento de modificação do eutético da liga AA356

The AA356 alloy is an alloy widely used in the automotive industry and aerospace due to its excellent mechanical properties. Refining the structure of eutectic silicon aluminum alloys is a fairly common practice in the foundry through treatment known as modification. This can be achieved by modifying agent adding chemicals such as contained in groups I and IIa of the periodic table and rare earths (europium, céreioi, praseodymium, neodymium, etc.). Has the ability to modify the structure of the eutectic, but only sodium and strontium produce an action modifier strong when used in low concentrations. The modifying effect of the shafts turn silicon into a fibrous form and branched surrounded by metallic matrix in the form of a composite structure that has the highest limit of tensile strength, ductility and machinability. In this work will be obtained ingots with and without the modifier type Al-10% Sr, made in sand molds and are generated and analyzed cooling curves and also the study of the macrostructure and microstructure of the solidified material. It was found that by adding the Al-Sr made shorten the solidification time and lower the grain size

Congelação de células espermáticas provenientes de epidídimo de gatos domésticos contendo antioxidante no meio diluidor

Pós-graduação em Medicina Veterinária - FCAV

Caracterização de cerâmicas a base de nitreto de silício para aplicações estruturais

The need for development of new materials is a natural process in the companies’ technological point of view, seeking improvements in materials and processes. Specifically, among the materials, ceramic exhibit valuable properties, especially the covalent ceramics which have excellent properties for applications which requires the abrasion resistance, hardness, high temperatures, resistence, etc. being a material that has applications in several areas. Most studies are related to improvement of properties, specially fracture toughness that allows the expansion of its application. Among the most promising ceramic materials are silicon nitride (Si3N4) which has excellent properties. The goal of this work was the development and caracterization of Si3N4-based ceramics, doped with yttrium oxide (Y2O3), rar earth concentrate (CTR2O3) and cerium oxide (CeO2) in the same proportion for the evaluation of properties. The powders' mixtures were homogenized, dried and compressed under pressure uniaxial and isostatic. Sintering was carried out in 1850 ⁰C under pressure of 0,1MPa N2 for 1 h with a heating rate of 25 ⁰C / min and cooling in the furnace inertia. The characterizations were performed using Archimedes principle to relative density, weight loss by measuring before and after sintering, phase analysis by X-ray diffraction, microstructure by scanning electron microscope (SEM), hardness and fracture toughness by the method Vickers indentation. The results obtained showed relative density of 97-98%, Vickers hardness 17 to 19 GPa, fracture toughness 5.6 to 6.8 MPa.m1/2, with phases varying from α-SiAlON and β-Si3N4 depending the types of additives used. The results are promising for tribological applications and can be defined according to the types of additives to be used

Nanotubos óxidos aplicados a resinas compostas fotoativadas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1 M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1 M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.

Investigação das propriedades ópticas, morfológicas e elétricas da heterojunção SnO2: Ce3+/ GaAs

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dissolução redutiva de minério de ferro por Acidithiobacillus ferrooxidans para a recuperação de metais de interesse econômico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo e desenvolvimento de filmes espessos de CeO2 obtidos por tape casting

Tape Casting proved to be an effective method for the production of thick films of CeO2 pure and doped with La. For this study, the nanoparticles used to form the slurry were synthesizes by the H-M method, at 100°C for 8 minutes, using KOH mineralizer. The slurry was made in aqueous solvent, requiring optimal control of surroundings conditions so that the produced tape has conditions to be studied. However, there's no toxicity or flammability in the film made by such solvent, being pleasing to the environment. The structural, optical and electrical properties of the films obtained by the Tape Casting process were studied by the methods of X-ray diffraction, scanning electron microscopy, specific surface area, Ultraviolet-visible spectroscopy and voltage-current measures, varying the electric field and frequency. From the results obtained by laboratory experiments, based on the literature, it was possible to reveal and understand some CeO2 features pure and doped with La

Estudo e desenvolvimento de filmes espessos de CeO2 obtidos por tape casting

Tape Casting proved to be an effective method for the production of thick films of CeO2 pure and doped with La. For this study, the nanoparticles used to form the slurry were synthesizes by the H-M method, at 100°C for 8 minutes, using KOH mineralizer. The slurry was made in aqueous solvent, requiring optimal control of surroundings conditions so that the produced tape has conditions to be studied. However, there's no toxicity or flammability in the film made by such solvent, being pleasing to the environment. The structural, optical and electrical properties of the films obtained by the Tape Casting process were studied by the methods of X-ray diffraction, scanning electron microscopy, specific surface area, Ultraviolet-visible spectroscopy and voltage-current measures, varying the electric field and frequency. From the results obtained by laboratory experiments, based on the literature, it was possible to reveal and understand some CeO2 features pure and doped with La