Impact of ocean acidification and warming on respiration, heart rate and acid-base status of Mytilus edulis from the North Sea

Anthropogenic climate change confronts marine organisms with rapid trends of concomitant warming and CO2 induced ocean acidification. The survival and distribution of species partly depend on their ability to exploit their physiological plasticity during acclimatization. Therefore, in laboratory studies the effects of simulated future ocean acidification on thermal tolerance, energy metabolism and acid-base regulation capacity of the North Sea population of the blue mussel Mytilus edulis were examined. Following one month of pre-acclimation to 10 °C and control CO2 levels, mussels were exposed for two weeks to control and projected oceanic CO2 levels (390, 750 and 1120 µatm) before being subjected to a stepwise warming protocol between 10 °C and 31 °C (+ 3 °C each night). Oxygen consumption and heart rates, anaerobic metabolite levels and haemolymph acid-base status were determined at each temperature. CO2 exposure left oxygen consumption rate unchanged at acclimation temperature but caused a somewhat stronger increase during acute warming and thus mildly higher Q10-values than seen in controls. Interestingly, the thermally induced limitation of oxygen consumption rate set in earlier in normocapnic than in hypercapnic (1120 µatm CO2) mussels (25.2 °C vs. 28.8 °C), likely due to an onset of metabolic depression in the control group following warming. However, the temperature induced increase in heart rate became limited above 25 °C in both groups indicating an unchanged pejus temperature regardless of CO2 treatment. An upper critical temperature was reached above 28 °C in both treatments indicated by the accumulation of anaerobic metabolites in the mantle tissue, paralleled by a strong increase in haemolymph PCO2 at 31 °C. Ocean acidification caused a decrease in haemolymph pH. The extracellular acidosis remained largely uncompensated despite some bicarbonate accumulation. In all treatments animals developed a progressive warming-induced extracellular acidosis. A stronger pH drop at around 25 °C was followed by stagnating heart rates. However, normocapnic mussels enhanced bicarbonate accumulation at the critical limit, a strategy no longer available to hypercapnic mussels. In conclusion, CO2 has small effects on the response patterns of mussels to warming, leaving thermal thresholds largely unaffected. High resilience of adult North Sea mussels to future ocean acidification indicates that sensitivity to thermal stress is more relevant in shaping the response to future climate change.

Flow conditions, bed form dimensions and shear stress experiments

Large asymmetric bed forms commonly develop in rivers. The turbulence associated with flow separation that develops over their steep lee side is responsible for the form shear stress which can represent a substantial part of total shear stress in rivers. This paper uses the Delft3D modeling system to investigate the effects of bed form geometry and forcing conditions on flow separation length and associated turbulence, and bed form shear stress over angle-of-repose (30 lee side angle) bed forms. The model was validated with lab measurements that showed sufficient agreement to be used for a systematic analysis. The influence of flow velocity, bed roughness, relative height (bed form height/water depth), and aspect ratio (bed form height/length) on the variations of the normalized length of the flow separation zone, the extent of the wake region (where the turbulent kinetic energy (TKE) was more than 70% of the maximum TKE), the average TKE within the wake region and the form shear stress were investigated. Form shear stress was found not to scale with the size of the flow separation zone but to be related to the product of the normalized extent of the wake region (extent of the wake region/extent of water body above the bed form) and the average TKE within the wake region. The results add to understanding of the hydrodynamics of bed forms and may be used for the development of better parameterizations of smallscale processes for application in large-scale studies.

Desarrollo metodológico de evaluación y aplicación de la sostenibilidad como base en los modelos educativos, para la evolución e innovación de la enseñanza de la arquitectura: modelo educativo de estudio México y España

La arquitectura y la construcción deben generar un bien común para la sociedad y medio ambiente, los arquitectos tienen la responsabilidad de mitigar muchos efectos negativos que se generan en esta profesión; esto no es posible si los estudiantes egresan con un conocimiento nulo sobre la arquitectura sostenible; por lo que surge la inquietud de desarrollar la presente tesis, con el objetivo de aportar de una forma sutil al desarrollo de la arquitectura compartiendo conocimiento para generar conocimiento, ya que la investigación que a continuación se desarrolla está enfocada al desarrollo de la arquitectura sostenible en el campo de la formación de los estudiantes, donde se pueda enlazar los nuevos requerimientos profesionales planteados desde la sostenibilidad. La formación del arquitecto ha intentado abordar el conocimiento humanístico, técnico, cultural, tecnológico, calculo estructural, instalaciones y construcciones; sin embargo, se ha dejado a un lado lo que abarca la arquitectura sostenible, como calidad de vida, diseño bioclimático, energías renovables, normativas sostenibles, economía viable, emisiones, contaminación y residuos generados, materiales, elementos constructivos sostenibles, mancha urbana, huella ecológica, impacto ambiental y análisis del ciclo de vida, entre otras estrategias o elementos. A través de la investigación científica y profunda que se ha realizado, se busca conformar el conocimiento sistemático que contribuya a la resolución de los problemas de nuestra sociedad, ambiente y educación, con la elaboración de un sistema metodológico de evaluación y aplicación de sostenibilidad en los modelos educativos. Para desarrollar el sistema de evaluación y aplicación, se desarrolla una metodología de investigación donde se justifica la necesidad de la creación de dicho sistema, en base al análisis de la situación actual del medio ambiente y la relación directa con la arquitectura, construcción y conocimientos adquiridos en la formación de los estudiantes de arquitectura, donde se demuestra la importancia de la educación de la arquitectura sostenible en el desarrollo de las sociedades. En base al análisis de metodologías y sistemas que evalúan la sostenibilidad de los edificios y los sistemas que evalúan la educación, se propone uno propio para evaluar las asignaturas de los modelos educativos en base a elementos sostenibles propuestos. La presente investigación se enmarca en una estrategia general de promover la evaluación y aplicación de la sostenibilidad en los modelos educativos de las escuelas de arquitectura a nivel internacional; como caso de estudio se evaluará el plan de estudios llamado Minerva, de la Benemérita Universidad Autónoma de Puebla en México, donde he realizado una estancia de investigación y la Escuela Técnica Superior de Arquitectura de la Universidad Politécnica de Madrid en España, donde he cursado el doctorado. ABSTRACT Architecture and construction must generate a common good for the society and environment, the architects have the responsibility of mitigating many negative effects that are generated in this profession; this is not possible if the students graduate with a void knowledge on the sustainable architecture that is why the concern for developing the present thesis, with the aim to contribute to the development of the architecture sharing knowledge to generate knowledge, seeing as the investigation that later develops is focused on the development of the sustainable architecture in about the student’s professional training, where it could connect the new professional requirements raised from the sustainability. The architect’s professional training has tried to approach the humanistic, technical, cultural, technological knowledge, structural calculation, fittings and constructions; nevertheless, there has been left aside what includes the sustainable architecture, as quality of life, design bioclimatic, renewable energies, sustainable regulations, viable economy, emission, pollution and generated, material residues, constructive sustainable elements, urban spot, ecological fingerprint, environmental impact and analysis of the life cycle, between other strategies or elements. Across the scientific and deep research that has been realized, it reaches to make up the systematic knowledge that he contributes to the resolution of the problems of our society, environment and education, with the production of a methodological system of evaluation and application of sustainability in the educational models. To develop the system of evaluation and application, there is a methodology of research where it justifies itself the need of the creation of the above mentioned system, on the basis of the analysis of the current situation of the environment and the direct relationship with the architecture, construction and knowledge acquired in the architecture student’s education, where there is demonstrated the matter of the education of the sustainable architecture in the development of the companies. Based on the analysis of methodologies and systems that evaluate the sustainability of the buildings and the systems that evaluate the education, there is one own proposes to evaluate the subjects of the educational models on the basis of sustainable proposed elements. The present research places in a general strategy of promoting the evaluation and application of the sustainability in the educational models of the schools of architecture worldwide; since case of study will evaluate the study plan called Minerva, of the Meritorious Autonomous University of It Populates in Mexico, where I have realized a stay of researching and the Technical Top School of Architecture of the Technical University of Madrid in Spain, where the PHD has been done.

Determinación de los parámetros de diseño a aplicar al proyecto de grandes yates a motor, en base a nuevos requerimientos de eficiencia energética y protección del medioambiente marino

Durante los últimos años, la construcción de grandes yates ha evolucionado hacia conceptos y diseños más complejos dónde se ha priorizado en muchas ocasiones la estética arquitectónica y exigencias de confort de los armadores y operadores dejando en segundo plano aspectos clave de seguridad. Diferentes Organismos Internacionales y las Sociedades de Clasificación han venido adaptando sus requisitos a la problemática específica de este tipo de buques, tratando de compatibilizar tendencias de diseño con exigencias de resistencia, integridad estructural, estanqueidad y seguridad entre otras. En la actualidad, la construcción de grandes yates con esloras incluso por encima de los 100 metros, el aumento del número de pasajeros por encima del límite tradicional de 12, las nuevas tendencias de ahorro energético y protección medioambiental que se están implantando en la industria en general y marítima en particular, plantean una serie de desafíos tanto a los diseñadores como a las Sociedades de Clasificación que deben avanzar en sus reglamentaciones para cubrir estos y otros aspectos. Son precisamente estos aspectos medioambientales, tradicionalmente relegados en la industria de grandes yates los que están ocupando en la actualidad un primer plano en los desarrollos de normativa de diferentes Organismos Internacionales y Nacionales. El impacto que estas nuevas normativas van a tener sobre el diseño de grandes yates a motor centra el desarrollo de esta Tesis. Hasta donde ha podido conocer el doctorando, esta es la primera vez que en una Tesis Doctoral se abordan los principales mecanismos que regulan el diseño y la construcción de grandes yates a motor, se estudian y analizan las regulaciones internacionales en materia de protección medioambiental y de eficiencia energética aplicables a los yates, se seleccionan y describen un conjunto de tecnologías maduras de carácter medioambiental, susceptibles de ser empleadas en yates y se determina los parámetros y aspectos del diseño a aplicar al proyecto de grandes yates a motor como resultado de la aplicación de estas tecnologías, analizados bajo la perspectiva de la Sociedad de Clasificación y de los Organismos Internacionales. La Tesis comienza con un análisis de la industria de construcción de grandes yates, la flota existente de grandes yates, la cartera actual de pedidos y la evolución esperada del mercado. Aquí se pone de manifiesto que a pesar de la crisis económica global de los últimos años, este mercado goza relativamente de buena salud y las previsiones son favorables, particularmente para el sector en Europa. A continuación se aborda el estado del arte del diseño de yate grande, sus peculiaridades, particularmente estructurales y de armamento, que le diferencian de otros tipos de buques y las tendencias en su diseño. Se pone de manifiesto cómo el proyecto de estos yates ha evolucionado hacia yates de gran tamaño y complejidad técnica, debido a la demanda y necesidades actuales y cómo ha influido en aspectos como la disposición estructural. Seguidamente se describen los principales mecanismos que regulan el diseño y construcción de grandes yates, particularmente el Código de Grandes Yates Comerciales de la Maritime & Coastguard Agency del Reino Unido, y las Reglas y Reglamentos de la Sociedad de Clasificación Lloyd’s Register para la Clasificación de yates; por ser ambas organizaciones las que lideran el Registro y la Clasificación respectivamente de este tipo de buques, objeto del estudio. El doctorando ejerce su actividad profesional como inspector de Lloyd’s Register en una oficina técnica de apoyo y evaluación de diseño, siendo especialista en grandes yates, lo que ha permitido exponer de primera mano el punto de vista de la Sociedad de Clasificación. En el siguiente Capítulo se describen las principales reglamentaciones internacionales de carácter medioambiental que afectan al diseño, construcción y operación de los yates, algunas de las cuales, como es el caso del Convenio Internacional para el Control y la Gestión del Agua de Lastre y Sedimentos de los buques (BWM 2004) aún no ha entrado en vigor a la fecha de terminación de esta Tesis. Seguidamente se realiza una selección de tecnologías desde el punto de vista de protección medioambiental y ahorro energético y su aplicación al diseño y construcción de grandes yates. Algunas de estas tecnologías son maduras y ya habían sido utilizadas con éxito en otros tipos de buques, pero su aplicación a los yates entraña ciertos desafíos que se describen en este Capítulo. A continuación se determinan y analizan los principales parámetros de diseño de los yates grandes a motor como consecuencia de las tecnologías estudiadas y se indican una serie de aspectos de diseño bajo la perspectiva de la Sociedad de Clasificación y de los Organismos Marítimos Internacionales. Finalmente se llega a una serie de conclusiones y se identifican futuras líneas de investigación en relación a las tecnologías descritas en este trabajo. ABSTRACT In recent years, the building of large yachts has evolved into more complex concepts and designs where often prioritized architectural aesthetics and comfort requirements of owners and operators leaving in the background key security aspects. Several international organizations and classification societies have been adapting their requirements to the specific problems of this type of vessel, trying to reconcile demands design trends with resistance, structural integrity, watertightness and safety among others. At present, the building of large yachts with lengths even above 100 meters, the increase in passenger numbers over the traditional limit of 12, new trends of energy saving and environmental protection are being implemented in the marine industry in particular, they pose a number of challenges to both designers and classification societies that should update and improve their regulations to cover these and other aspects. It is precisely these environmental issues, traditionally relegated to the large yacht industry, which are currently occupying center stage in the development of rules of different international and national bodies. The impact that these new standards will have on the design of large motor yachts focuses the development of this thesis. As far as it is known, this is the first time in a doctoral thesis the main mechanisms regulating the design and construction of large motor yachts are addressed, the international regulations on environmental protection and energy efficiency requirements for yachts are studied and analyzed, a set of mature environmental technologies, capable of being applied to yachts are selected and described, the parameters and design aspects to be applied to large yacht projects as a result of the application of these technologies are determined and analyzed from the perspective of the Classification Society and international organizations. The thesis begins with an analysis of the shipbuilding industry of large yachts, the existing fleet of large yachts, the current backlog and the expected market developments. Here it becomes clear that despite the global economic crisis of recent years, this market enjoys relatively good health and prospects are favorable, particularly for the sector in Europe. Then the state of the art of large yacht design, its peculiarities, particularly structural and outfitting, that differentiate it from other types of ships and trends in design is discussed. It shows how the project of these yachts has evolved to large yachts and technical complexity, due to the demand and needs and how it has influenced the structural arrangement aspects. Then the main mechanisms regulating the design and construction of large yachts, particularly the Large Commercial Yacht Code developed by the Maritime & Coastguard Agency (UK) and the Lloyd’s Register Rules & Regulations for the Classification of Special Service Craft including yachts are described; the two organizations to be leading the registration and classification respectively of such vessels under study. The doctoral student practices his profession as a senior specialist to Lloyd’s Register in a technical support office, dealing with the design assessment of large yachts, which allowed exposing firsthand view of the Classification Society. In the next chapter describes the main international environmental regulations, affecting the design, construction and operation of yachts, some of which, such as the International Convention for the Control and Management of Ships' Ballast Water and Sediments (BWM 2004) has not yet entered into force at the date of completion of this thesis. Following is a selection of technologies from the point of view of environmental protection and energy saving and its application to design and construction of large yachts. Some of these technologies are mature and have already been used successfully in other ship types, but their application to yachts entails certain challenges that are described in this chapter. Then identifies and analyzes the main design parameters of large motor yachts as a result of the technologies studied and a number of design aspects are given from the perspective of Classification Society and international maritime organizations. Finally, a number of conclusions are exposed, and future research is identified in relation to the technologies described in this Thesis.

Propriedades superficiais de filmes à base de gelatina

O objetivo do presente trabalho foi caracterizar as propriedades superficiais de filmes à base de gelatina. Para o qual foram elaborados filmes de: (i) Gelatina plastificado com glicerol (G) (gelatina: 5 g/100 g SFF; glicerol: 30 g/100 g de gelatina), (ii) Gelatina reforçado com montmorilhonita (G/MMT) (gelatina: 5 g/100 g SFF; glicerol: 30 g/100 g de gelatina; MMT: 5 g/100 g de gelatina) e Gelatina plastificado com citrato de acetiltributila (G/ATB) (gelatina: 5 g/100 g SFF; ATB: 50 g/100 de gelatina; lecitina de soja: 60 g/100 g de ATB; etanol: 20 g/100 g SFF). Os filmes foram produzidos mediante o uso de um aplicador automático de filmes \"Spreading\". Logo, os filmes foram submetidos a testes para determinação da espessura, umidade e propriedades óticas (brilho, cor e opacidade). Também foi caracterizada a microestrutura por microscopia eletrônica de varredura (MEV) e microscopia de força atômica (AFM); às imagens obtidas por MEV foi aplicado um analise de imagem mediante o programa Image J, para obter o valor da dimensão fractal (DF). Depois foram caracterizadas as propriedades superficiais de ângulo de contato (AC), molhabilidade ou coeficiente de espalhamento (Se), e energia livre superficial (ELS) mediante a medida do ângulo de contato pelo método da gota séssil (água: 5 µL e 1-Bromonaftaleno: 3 µL). Para o cálculo da ELS também foi aplicado o método de Owens-Wendt. Estas caracterizações foram feitas em ambos os lados do filme, lado ar e lado placa. A natureza do filme de G/ATB influenciou na umidade e as propriedades óticas, enquanto que os filmes de G e G/MMT apresentaram características similares. Em relação à microestrutura e rugosidade, o filme de G apresentou a superfície mais homogênea e lisa, contrario ao observado no filme de G/MMT, que apresentou a maior rugosidade seguida do filme de G/ATB. Foi observado que houve uma relação entre os valores de rugosidade e DF. De acordo com o valor do AC, os filmes apresentaram um caráter hidrofóbico, pois seus valores foram superiores a 65° (em ambos os lados dos filmes), na seguinte ordem: G/MMT > G > G/ATB; sendo que o Se seguiu a mesma tendência. Cabe mencionar também que não foi encontrada uma correlação significativa entre os valores de AC e rugosidade. Em função dos valores de AC, Se e ELS (especificamente a componente polar), o filme de G/ATB apresentou o menor caráter hidrofóbico, pois apresentou menores valores de AC e maiores valores de Se em comparação com os outros dois filmes. Os valores da componente polar da G/ATB foram os maiores, explicando de melhor maneira o caráter menos hidrofóbico deste filme. Pode-se concluir que os filmes à base de gelatina elaborados no presente trabalho têm propriedades hidrofóbicas (AC>65°), sendo a G/MMT o filme com melhores características hidrofóbicas.

Cyclically-arranged, storm-controlled, prograding lithosomes in Messinian terrigenous shelves (Bajo Segura Basin, western Mediterranean)

This work focuses on a Messinian shallow-marine terrigenous unit, termed the La Virgen Formation, which forms part of the sedimentary infill of the Bajo Segura Basin (Betic margin of the western Mediterranean). This formation was deposited during a high sea level phase prior to the onset of the Messinian Salinity Crisis. Stratigraphically, it comprises a prograding stack of sandstone lithosomes alternating with marly intervals (1st-order cyclicity). These lithosomes are characterized by a homoclinal geometry that tapers distally, and interfinger with pelagic sediments rich in planktonic and benthic microfauna (Torremendo Formation). An analysis of sedimentary facies of each lithosome reveals a repetitive succession of sandy storm beds (tempestites), occasionally amalgamated, which are separated by thin marly layers (2nd-order cyclicity). Each storm bed contains internal erosional surfaces (3rd-order cyclicity) that delimit sets of laminae. Two categories of storm beds have been differentiated. The first one includes layers formed below storm wave base (SWB), characterized by traction structures associated to unidirectional flows (scoured base, planar lamination, and parting lineation). The second category consists of layers deposited above the SWB which display typical high regime oscillatory flow structures (swaley and hummocky cross lamination). In both cases, the ichnological record is characterized by an oligotypic association of Ophiomorpha nodosa, which can be interpreted as the result of allochthonous tracemakers (crustaceans) transported during storm events together with the sediment. The benthic microfauna in the marly intervals that separate the sandstone lithosomes (1st-order cyclicity) indicates that the storm ebb surges were deposited at depths ranging from those of inner shelf settings (with Elphidium spp. and Cibicides lobatulus) to those of outer shelf (with Valvulineria complanata and Uvigerina cylindrica). At the distal end of the sandstone lithosomes, the planktonic microfauna is characterized by a high content of taxa indicative of warm-oligotrophic waters (Globigerinoides obliquus and Globigerinoides bulloideus). In contrast, in the marly intervals, the microfauna is dominated by species typical of cold-eutrophic waters (Globigerina and Neogloboquadrina). This alternation of planktic foraminiferal assemblages is interpreted as being the expression of climatic cycles, in which every episode of progradation of tempestite-dominated lithosomes corresponds to maximum insolation and warm waters, whereas episodes of marly deposition correspond to minimal insolation and cold waters. The 1st-order cyclicity recorded in the La Virgen Formation, in a context of terrigenous storm-dominated shelf, corresponds to sapropel/homogeneous marl cycles formed in a pelagic basin (Torremendo Fm). These cycles in pelagic sediments are commonplace throughout the Mediterranean during the Messinian and reflect precession orbital changes: repeated periods of maximum insolation – minimum precession (sapropels) and minimal insolation – maximum precession (homogeneous marls). The fact that the example of terrigenous unit studied herein is coetaneous with the well-developed reef complexes in the Mediterranean basins points out the importance of sediment supply in the formation of large-scale sandy lithosomes. This is a crucial aspect to understanding reservoir genesis as well as lateral stratigraphic relationships with potential seal and/or source rocks.