Blockade of T- and B-lymphocyte development by antibody to the gamma c subunit of the receptors for interleukins 2, 4, and 7.

Cytokines are important regulators of hematopoesis. Mutations in gamma c, which is a subunit shared by the receptors for interleukin (IL) 2, IL-4, and IL-7, have been causally associated with human X chromosome-linked severe combined immunodeficiency disease. This finding indicates a mandatory role for cytokine receptor signaling at one or more stages of lymphocyte development. To evaluate the cellular level at which gamma c is critical for lymphopoiesis, the effect of monoclonal antibodies to gamma c on the capacity of syngeneic bone marrow cells to reconstitute the hematopoietic compartment of lethally irradiated recipient mice was examined. We show that monoclonal antibody to gamma c blocked lymphocyte development at or before the appearance of pro-B cells and prior to or at the seeding of the thymus by precursor cells while erythromyeloid cell development was normal. These results suggest that one level of lymphocyte development that requires gamma c is a point in hematopoietic cell differentiation near the divergence of lymphopoiesis and erythromyelopoesis.

Proinsulin slows retinal degeneration and vision loss in the P23H rat model of retinitis pigmentosa

Proinsulin has been characterized as a neuroprotective molecule. In this work we assess the therapeutic potential of proinsulin on photoreceptor degeneration, synaptic connectivity, and functional activity of the retina in the transgenic P23H rat, an animal model of autosomal dominant retinitis pigmentosa (RP). P23H homozygous rats received an intramuscular injection of an adeno-associated viral vector serotype 1 (AAV1) expressing human proinsulin (hPi+) or AAV1-null vector (hPi−) at P20. Levels of hPi in serum were determined by enzyme-linked immunosorbent assay (ELISA), and visual function was evaluated by electroretinographic (ERG) recording at P30, P60, P90, and P120. Preservation of retinal structure was assessed by immunohistochemistry at P120. Human proinsulin was detected in serum from rats injected with hPi+ at all times tested, with average hPi levels ranging from 1.1 nM (P30) to 1.4 nM (P120). ERG recordings showed an amelioration of vision loss in hPi+ animals. The scotopic b-waves were significantly higher in hPi+ animals than in control rats at P90 and P120. This attenuation of visual deterioration correlated with a delay in photoreceptor degeneration and the preservation of retinal cytoarchitecture. hPi+ animals had 48.7% more photoreceptors than control animals. Presynaptic and postsynaptic elements, as well as the synaptic contacts between photoreceptors and bipolar or horizontal cells, were preserved in hPi+ P23H rats. Furthermore, in hPi+ rat retinas the number of rod bipolar cell bodies was greater than in control rats. Our data demonstrate that hPi expression preserves cone and rod structure and function, together with their contacts with postsynaptic neurons, in the P23H rat. These data strongly support the further development of proinsulin-based therapy to counteract retinitis pigmentosa.

Reports of cases argued and determined in the Supreme Judicial Court of New-Hampshire.

Vols. for 1855-59 reported by G.G. Fogg; 1859-63 by W.E. Chandler; 1863-69 by A. Hadley; 1869-74 by J. M. Shirley.

Mobility of people and goods in the urban environment: mobility of the handicapped and elderly. Second draft final report (second year).

Transportation Department, Office of University Research, Washington, D.C.

Steam warfare in the Parana: a narrative of operations, by the combined squadrons of England and France, in forcing a passage up that river,

Sketches of South America, illustrative of allusions in the foregoing narrative, to the geography, natural history, inhabitants, etc., of that part of the world. Extracts translated from Felix de Azara's Voyages dans l'Amérique Méridionale... v.2, p. [121]-287.

Reconnaissance for uranium in the Ayacucho Basin, departments of Huancavelica and Ayacucho, Peru /

In cooperation with the Junta de Control de Energía Atómica del Peru.

Temperature variation, heat transfer, and void volume development in the transient atmospheric boiling of water /

"Series No. 163, Issue No. 2"--Cover.

Essays in the earlier history of American corporations.

Bibliography: v. 2, p. 347-395.

Case of the slave-child, Med : report of the arguments of counsel, and of the opinion of the court, in the case of Commonwealth vs. Aves; tried and determined in the Supreme judicial court of Massachusetts.

Med's case.--Arguments of counsel; Benjamin R. Curtis. esq., for the respondent, Ellis Gray Loring, esq., for the petitioner, Hon. Rufus Choate, for the petitioner, Charles P. Curtis, esq., for the respondent.--Opinion of the court.

An eulogium upon Benjamin Rush, M.D. : professor of the institutes and practice of medicine and of clinical practice in the University of Pennsylvania : who departed this life April 19, 1813, in the sixty-ninth year of his age : written at the request of the Medical Society of South Carolina, and delivered before them and others, in the Circular Church of Charleston, on the 10th of June, 1813, and pub. at their request /

Microfilm. Ann Arbor, Mich. : Xerox University Microfilms -- 1 reel ; 35 mm.

A funeral sermon, delivered at Loudon, January 24, 1810, at the interment of Mr. Abner Ames D'Wolf, : a member of Williams College, who died at Williamstown, January 20th, in the twenty second year of his age, and the third year of his collegiate life. /

Title vignette.

The study and practice of the law, considered in their various relations to society. In a series of letters.

Mode of access: Internet.

The bow in the cloud : or, words of comfort for those in bereavement, sickness, sorrow, and the varied trials of life /

Mode of access: Internet.

The theory and practice of bank book-keeping, and joint stock accounts; exemplified and elucidated in a complete set of bank account books, (printed in colors,) arranged in accordance with the principles of double entry ...

Mode of access: Internet.

The theory and practice of surveying. Designed for the use of surveyors and engineers generally, but especially for the use of students in engineering.

Mode of access: Internet.