2C-Cas9: a versatile tool for clonal analysis of gene function

CRISPR/Cas9-mediated targeted mutagenesis allows efficient generation of loss-of-function alleles in zebrafish. To date this technology has been primarily used to generate genetic knockout animals. Nevertheless, the study of the function of certain loci might require tight spatiotemporal control of gene inactivation. Here, we show that tissue-specific gene disruption can be achieved by driving Cas9 expression with the Gal4/UAS system. Furthermore, by combining the Gal4/UAS and Cre/loxP systems, we establish a versatile tool to genetically label mutant cell clones, enabling their phenotypic analysis. Our technique has the potential to be applied to diverse model organisms, enabling tissue-specific loss-of-function and phenotypic characterization of live and fixed tissues.

Annals of Ulster : or, Ireland fifty years ago /

Mode of access: Internet.

About Ulster.

Mode of access: Internet.

Across an Ulster bog /

Mode of access: Internet.

Drainage basins of streams entering the Hudson River in Orange, Ulster, Dutchess and Putnam Counties, except Rondout Creek below Rondout Reservoir, Esopus Creek below Ashokan Reservoir, Wappinger Creek, and waters contained within boundaries of State-owned forest preserve lands; official classifications.

Cover title.

The history of the 36th (Ulster) Division /

Includes index.

Ulster,

Map on lining-paper.

Cuchulain of Muirthemme: the story of the men of the Red Branch of Ulster;

Mode of access: Internet.

Ulster journal of archaeology.

"Ulster bibliography" included in 2d ser., v. 6-

Captain's Cuellar's adventures in Connacht & Ulster A.D. 1588 : a picture of the times, drawn from contemporary sources : To which is added an introduction and complete translation of Captain Cuellar's Narrative of the Spanish Armada and his adventures in Ireland /

Based on the author's paper published in the Ulster journal of archeology, v.1, pt.3, April 1895 under title: Spanish Armada in Ulster and Connacht.

The West Ulster Staffords and their descendants.

Mode of access: Internet.