Influence of condensed tannins from Brazilian semi-arid legumes on ruminal degradability, microbial colonization and ruminal enzymatic activity in Saanen goats

The present study aimed at determining the influence of condensed tannins present in the Brazilian legume species Mimosa hostilis, Mimosa caesalpinifolia and Bauhinia cheilantha on ruminal degradability, microbial colonization and enzymatic activity. Polyethylene glycol (PEG) was used to reduce the astringency and concentration of soluble condensed tannins. Four ruminally-cannulated Saanen goats (60 +/- 8 kg BW) were fed, in two experimental periods, with a hay diet based on the studied legumes treated or non-treated with PEG. Voluntary intake, microbial colonization, DM, CP, NDF, and ruminal degradability of PEG treated and non-treated forage leaves, as well as pH, ammonia and 1,4 P-endoglucanase activity of the rumen content were evaluated. Astringency and soluble tannin concentration of the studied legumes were reduced by approximately 70% and 50%, respectively, with PEG treatment. Average DM intake was higher for the treated diet (16.76 g DM/kg BW/day against 13.06 g DM/kg BW/day). Percentile values for degradation parameters and for potential and effective degradabilities of DM, CP and NDF were also affected by the tannins, but at different intensities. Electron microscopic observations of ruminally-incubated legume leaves showed a more effective microbial colonization of PEG-treated leaves for all legume species. A decrease in pH and an increase in ammonia concentration and in endoglucanase activity in the ruminal content was also observed for PEG-treated diets at all sampling periods. Condensed tannins of the studied legume species have influenced the adhesion conditions, colonization and enzymatic activity of the microbial ecosystem, and consequently the ruminal degradation of the different dietary fractions. For this reason, the reduction in condensed tannin would be of great importance to improve the nutrition of ruminant feeding of these species. (c) 2005 Elsevier B.V. All rights reserved.

New Neotropical cunaxine species (Acari: Prostigmata: Cunaxidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Methodology for kariological study of Brazilian Palms

In the laboratory of cytogenetics of the DBAA-UNESP we are studying the karyotipe of some Brazilian Palms. To determine the best protocol, methods of seed germination, inhibition of mitosis, time to pick up the roots and staining were analyzed. The results shown that the seed germination in sphagnum is effective to achieve good roots. The best time to collect the root tips is between 11 to 12 AM., when there are more cell metaphases. The inhibition of mitosis cycle at metaphases may be effective both with 8-hydroxiquinoleine (0,03% -5 hours) or with cold water (0°C - 18-20 hours). The staining with Giemsa 2% showed the best chromosome figures in the metaphases. Now, to get good metaphases slides the method in use in the lab is: 1) seed germination in sphagnum at room temperature and high humidity; 2) The major roots are cut maintaining at least 5 cm, because this technic allows new emergence of roots, increasing the number of roots collected per germinated seed, that is very important in some species with poor germination rates; 3) To get the mitosis inhibition we are using cold water (0°C) treatment for 18-20 hours, following the standard protocols for conservation and hydrolysis; or enzyme digestion with pectinasecellulase 4) the staining procedures are made using Giemsa 2%. The Brazilian palms species studied and their respective chromosome number were: Aiphanes acanthophylla (2n=30), A. caryotaefolia (2n=30), Syagrus quinquifaria (2n=32), S. coronata (2n=32), S. romanzoffiana (2n=32), Euterpe edulis (2n=36), E. oleracea (2n=36), Copernicia prunifera (2n=36), Scheelea lauromuelleriana (2n=32) and Bactris gasipaes (2n=30).

Dynamics of handling time and functional response by larvae of Chrysomya albiceps (Dipt., Calliphoridae) on different prey species

One way to understand the behavioural patterns exhibited by a predator in response to prey density is to evaluate its functional response. Such evaluation yields information about basic mechanisms of prey-predator dynamics, and is an essential component of prey-predator models. In this paper we analysed experimentally the functional response and the handling time spent by Chrysomya albiceps on different prey species and larval instars of blowflies. The type II functional response was observed when second instar larvae of Chrysomya megacephala and Chrysomya macellaria were consumed. The handling time spent by the predator was significantly different between instars and species. The implications of the functional response and handling time for the interaction dynamics of Brazilian Chrysomyinae species are discussed.

Genetic conservation of Brazilian fishes - Present state and perspectives

Natural environments have been worldwide affected by the growing impact of anthropogenic actions that promote the reduction or the extinction of several vertebrate species. Aquatic ecosystems represent one of the most affected environments and many fish species and/or populations have been increasingly fragmented distributed due to habitat degradation, predatory fishing, introduction of exotic species, river sedimentation, deforestation, pollution, reduction of food resource, and construction of hydroelectric dams. Actually, more than 150 Brazilian fish species, including freshwater, estuary and coastal species, can be considered threatened. Information on the diversity, conservation biology and population analysis on threatened species or populations, with several DNA markers, can be extremely useful for the success of fish species-recovery and maintenance programs. Although DNA analysis in Neotropical fish species are just beginning, they tend to increase with the widespread attention to the use of molecular approaches to minimize problems related to the risk of extinction. The accumulation of information on biology and pattern of genetic variation of fish species, associated with ecological and demographic data, and also education and respect to the nature, constitutes a crucial task to develop efficient conservation strategies in order to preserve the genetic diversity in aquatic environments.

Diversidade de mamíferos de médio e grande porte e sua relação com o mosaico vegetacional na fazenda experimental Edgárdia, Unesp, Botucatu/SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O emprego da geoestatística na determinação do tamanho "ótimo" de amostras aleatórias com vistas à obtenção de estimativas dos volumes dos fustes de espécies florestais em Paragominas, estado do Pará

O objetivo do trabalho foi determinar o tamanho adequado de amostra para estimar o volume de fustes de espécies florestais de uma população de árvores a serem cortadas no sistema de manejo florestal da empresa Cikel Brasil Verde Madeiras - Pará. Utilizaram-se as metodologias da amostragem sistemática e do estimador geoestatístico da krigagem ordinária com simulação sequencial, respectivamente para a escolha das amostras e estimação dos volumes dos fustes das árvores. Os resultados mostraram que os métodos podem ser utilizados no cálculo dos volumes de fustes de árvores. Entretanto, o método da krigagem apresenta um efeito de suavização, tendo como conseqüência uma subestimação dos volumes calculados. Neste caso, um fator de correção foi aplicado para minimizar o efeito da suavização. A simulação sequencial indicativa apresentou resultados mais precisos em relação à krigagem, uma vez que tal método apresentou algumas vantagens, tal como a não exigência de amostras com distribuições normais e ausência de efeito de suavização, característico dos métodos de interpolação.

Leaf-litter decomposition of the mangrove species Avicennia schaueriana, Laguncularia racemosa and Rhizophora mangle

This study evaluated the decomposition process of leaf litter from the main Brazilian mangrove species Avicennia schaueriana, Laguncularia racemosa and Rhizophora mangle. Senescent leaves were collected, dried and placed in nylon bags with different mesh sizes (fine: 2x2mm and coarse: 8x8mm). The bags were distributed over the sediment, and replicates of each species and mesh size were collected periodically over 4months. In the laboratory, the dry weight of the samples was measured, and the decomposition coefficient (k) for each species and mesh size was obtained over time. All species showed a rapid decomposition rate at the beginning of the experiment, followed by a slower but steady rate of decomposition over time. The rate of leaf litter decomposition was highest in A. schaueriana, intermediate in L. racemosa and lowest in R. mangle. The difference was mainly linked to the activity and abundance of detritivores, together with the different litter quality of the species, which determined their palatability and probably influenced the decomposition process.

Comparação entre valores de ensaios experimentais e calculados da resistência ao embutimento da madeira de Pinus taeda L.

To design connections between timber parts with metal dowels (nails or bolts), two phenomena must be considered: bending of the metal dowel and the embedment strength in the wood. The Brazilian Standard ABNT NBR 7190:1997 proposes the methods used in the laboratory tests to determine the embedment strength with the metal dowell and, in the absence of testing, specifies relations to calculate the embedment strength from the compression strength. The aim of this research was to compare the embedment strength values obtained by experimental tests and calculated by the relationships established by the Brazilian Standard ABNT NBR 7190:1997. By the results of the hypothesis tests, it was possible to conclude that the estimate of the embedment strength parallel to the grain proposed by the Brazilian standard ABNT NBR 7190:1997, establishing equivalence with the strength compression results in the same direction, must be effective to the wood species Pinus taeda L., however, the same was not found in the perpendicular direction with respect to the grain, possibly explained by the alpha(e) values in the equation to calculed fe(90)

Pollen morphology of Fridericia Mart. (Bignoniaceae) from Brazilian forest fragments

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estratégias de propagação de barueiro (Dipteryx alata Vog.) e jatobazeiro do cerrado (Hymenaea stigonocarpa MART)

Pós-graduação em Agronomia - FEIS

A new probabilistic canopy dynamics model (SLCD) that is suitable for evergreen and deciduous forest ecosystems

There are strong uncertainties regarding LAI dynamics in forest ecosystems in response to climate change. While empirical growth & yield models (G&YMs) provide good estimations of tree growth at the stand level on a yearly to decennial scale, process-based models (PBMs) use LAI dynamics as a key variable for enabling the accurate prediction of tree growth over short time scales. Bridging the gap between PBMs and G&YMs could improve the prediction of forest growth and, therefore, carbon, water and nutrient fluxes by combining modeling approaches at the stand level.Our study aimed to estimate monthly changes of leaf area in response to climate variations from sparse measurements of foliage area and biomass. A leaf population probabilistic model (SLCD) was designed to simulate foliage renewal. The leaf population was distributed in monthly cohorts, and the total population size was limited depending on forest age and productivity. Foliage dynamics were driven by a foliation function and the probabilities ruling leaf aging or fall. Their formulation depends on the forest environment.The model was applied to three tree species growing under contrasting climates and soil types. In tropical Brazilian evergreen broadleaf eucalypt plantations, the phenology was described using 8 parameters. A multi-objective evolutionary algorithm method (MOEA) was used to fit the model parameters on litterfall and LAI data over an entire stand rotation. Field measurements from a second eucalypt stand were used to validate the model. Seasonal LAI changes were accurately rendered for both sites (R-2 = 0.898 adjustment, R-2 = 0.698 validation). Litterfall production was correctly simulated (R-2 = 0.562, R-2 = 0.4018 validation) and may be improved by using additional validation data in future work. In two French temperate deciduous forests (beech and oak), we adapted phenological sub-modules of the CASTANEA model to simulate canopy dynamics, and SLCD was validated using LAI measurements. The phenological patterns were simulated with good accuracy in the two cases studied. However, IA/max was not accurately simulated in the beech forest, and further improvement is required.Our probabilistic approach is expected to contribute to improving predictions of LAI dynamics. The model formalism is general and suitable to broadleaf forests for a large range of ecological conditions. (C) 2014 Elsevier B.V. All rights reserved.

Caracterização físico-mecânica de elementos estruturais roliços perfilados de Amaru

The study of physical and mechanical properties of wood is essential for its structural use and it is of great importance to the construction industry. Thus, this study aimed to determine the physical and mechanical properties of the wood Amaru - a hybrid of Eucalyptus, developed by Plantar Projects and Forest Products Ltda. In order to determine the properties of Amaru, round samples were used, which were provided to the Laboratory of Wood and Wooden Structures of the School of Engineering of São Carlos, University of São Paulo - LaMEM / EESC / USP. For the characterization of the physical properties, the apparent specific gravity and moisture content of the samples were determined. To the mechanical characterization, the following properties were evaluated: strength and stiffness in compression, strength and stiffness in bending, shear and tension. The procedures of the tests performed in this study were done according to the recommendations of the Brazilian Wood Standard ABNT NBR 7190:1997. The specimen used were confectioned in actual dimensions, according to as those used in the construction system proposed by Plantar. The results obtained from the tests performed showed that the mechanical properties approached the values proposed by the Wood Standard NBR 7190. The visual grading was important to provide a primary idea about the failure modes to be obtained from the tests performed. The bending test showed the modulus of elasticity (MOE) and Modulus of Rupture (MOR), which resulted in 15822 MPa and 101,7 MPa, respectively. The compression test resulted in values Ec0,m and fc0, 15698 MPa and 50,7 MPa. The tensile strength (ft0) of this hybrid was calculated and its value obtained was 60,8 MPa. The shear strength (fv0) was 8,2 MPa. The results obtained from the tests are the basis for engineers and architects to design structures using wood species Amaru

Natural compounds isolated from Brazilian plants are potent inhibitors of hepatitis C virus replication in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Wood and leaf anatomy of Copaifera langsdorffii dwarf trees

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)