Efeito da aplicação de piraclostrobina no crescimento de plantas jovens de ipê roxo

O ipê roxo é uma árvore de grande importância comercial e farmacológica, além de possuir grande beleza e, por esse motivo, ser muito utilizado em paisagismo. Apesar da existência de diferentes fungicidas no mercado, o uso das estrobilurinas, como a piraclostrobina, tem aumentado significativamente pelo seu efeito fisiológico, que altera o metabolismo da planta, acelerando o desenvolvimento da planta. Assim, neste trabalho analisou-se o crescimento de plantas jovens de Tabebuia avellanedae Lorentz ex Griseb em função da aplicação de piraclostrobina em diferentes concentrações. Os tratamentos foram realizados em intervalos de 30 dias com as seguintes concentrações do fungicida: piraclostrobina 0,375g L-1, piraclostronina 0,750g L-1 e piraclostrobina 1,125g L-1 e a testemunha. Foram avaliadas a altura das plantas, diâmetro do caule, número de folhas, massa seca das folhas, caule e raízes, bem como a avaliação de trocas gasosas e atividade enzimática. As plantas tratadas com piraclostrobina apresentaram incremento na massa seca, massa fresca e na taxa fotossintética na maioria dos tratamentos avaliados

Monitoramento do perfil microbiológico de dentes com infecção endodôntica primária durante terapia com diferentes medicações intracanal

Pós-graduação em Odontologia Restauradora - ICT

Insecticidal Effect of Extracts from Native Plants of Mato Grosso do Sul State on Sitophilus zeamais Mots (Coleoptera: Curculionidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Plantas medicinais do SUS: espécies comuns da região de Bauru-SP

This work deals with present and discuss mainly the chemical composition and pharmacological activities of each species of the National List of Medicinal plants of interest to SUS (RENISUS) contained in scientific articles that are found in the Bauru-SP region. Such information compiled in this study may help in the advancement of scientific research, promoting the speed in bibliographic queries these species. In the present work was carried out consultation papers and described in the form of literature review, published information of the species listed in Renisus specific to the Bauru-SP region are: Aloe spp* (A. vera or A. barbadensis), Schinus terebinthifolius = mastic Schinus, trimera Baccharis, Mikania spp* (M. glomerata and M. laevigata), Vernonia condensata, Tabebuia avellanedeae, Chenopodium ambrosioides, Momordica charantia, Phyllanthus spp* (P. amarus, P. niruri, P. tenellus and P. urinaria), Stryphnodendron adstringens = Stryphnodendron barbatimam, pulegium Mentha, Mentha spp* (M. crispa, M. piperita or M. villosa), Plectranthus barbatus = Coleus barbatus, Persea spp* (gratissima or P. americana P.), Bauhinia spp* (B. affinis, B. forficata or B. variegata), Copaifera spp*, Morus sp*, Eugenia uniflora or brasiliana Myrtus*, Psidium guajava, Syzygium spp* (S. jambolanum or S. cumini), Passiflora spp* (P. alata, P. edulis or P. incarnata), Punica granatum and Casearia sylvestris. Studies have shown that the use of plants as alternatives treatment and sustainable use of Brazilian biodiversity has a breakthrough in research regarding the chemical composition of each species of RENISUS relationship. Many phytochemical studies are reported compiled with possible pharmacological indications of each species. Thus enabling the use and production of herbal medicines in SUS

Viabilidade de sementes de Handroanthus heptaphyllus (Vell.) Mattos armazenadas nos frutos

Pós-graduação em Ciência Florestal - FCA

Armazenamento de sementes germinadas de Handroanthus heptaphyllus (Vell.) Mattos e seu comportamento em viveiro

Pós-graduação em Ciência Florestal - FCA

Microbiological Profile Resistant to Different Intracanal Medications in Primary Endodontic Infections

Introduction: This clinical study aimed to determine the microbiological profile resistant to different intracanal medications in primary endodontic infections by using both microbiological culture and the checkerboard DNA-DNA hybridization technique. Methods: Twenty primarily infected root canals were selected and then instrumented before being randomly divided into 2 groups according to the intracanal medications: calcium hydroxide (Ca[OH](2)) or Ca(OH)(2) + chlorhexidine (CHX). Samples were collected before and after root canal procedures, which consisted in submitting them to microbiological culture and processing them for checkerboard DNA-DNA hybridization. Results: No differences were found between the Ca(OH)(2) (99.98%) and Ca(OH)(2) + CHX groups (99.76%) regarding the median percentage values for the reduction of cultivable bacteria. The most frequently detected species were Capnocytophaga ochracea (70%) and Fusobacterium nucleatum ssp. vincentii (70%) in the initial samples. After instrumentation, the most frequently detected species were E. faecium (60%). After root canal treatments using either Ca(OH)(2) or Ca(OH)(2) + CHX as intracanal medications, the most frequently detected species were E nucleatum ssp. vincentii (90%) and Enterococcus faecium (40%), respectively. Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, this reduction was significantly greater in the Ca(OH)(2) + CHX group (P < .05). This difference was also observed when evaluating the total bacterial load (P < .05). Conclusions: The use of Ca(OH)(2) associated with CHX as an intracanal medication showed better results by acting on gram-positive and gram-negative microorganisms although such an action to eradicate enterococci should also be sought.

Influence of moisture content in some mechanical properties of two brazilian tropical wood species

This study aimed, with the aid of analysis of variance (ANOVA), to investigate and quantify the influence of moisture ranging between 12% and over 30% (fiber saturation) on the mechanical properties: strength and modulus of elasticity in compression and in tension parallel to grain; modulus of rupture and modulus of elasticity in static bending; shear strength parallel to grain considering wood species Ipê (Tabebuia sp) and Angelim Araroba (Vataireopsis araroba). Tests were performed according to the assumptions and calculating methods Brazilian standard ABNT NBR 7190, Anexx B, totalizing 400 tests. Results of ANOVA revealed a significant reduction (16% on average) for mechanical properties wood due to the increase in moisture content from 12% to over 30% (fiber saturation). The same behavior also occurred when assembly containing the two species was considered.

The Somatic Chromosomes of Some North American Marmots (Sciuridae), with Remarks on the Relationships of Marmota browerii Hall and Gilmore

Karyotypes are defined for two nearctic species of marmots, Marmota olympus (2n = 40) and M. vancouverensis (2n = 42), and supplemental information is included on the karyotypes of M. flaviventris, M. monax ochracea, and M. marmota. The six North American species of Marmota (NF = 66) comprise a distinct group as compared with the middle Asian species (NF = 70) for which the karyotypes are known. Karyologic findings and zoogeographic evidence based upon the distribution of two nearctic species of host-specific cestodes indicate that M. broweri, in northern Alaska, is a pre-Würm relict. Its affinities appear to be with the North American caligata-group rather than with the northeastern Siberian M. camtschatica. The occurrence on M. broweri of the Asian flea, Oropsylla silantiewi, has not been explained. Some ecological and behavioral characteristics of M. broweri are briefly described and compared with those of other species. Family groups of M. broweri hibernate together in single winter dens that are plugged at the entrance; copulation takes place before the animals emerge from the winter den, near mid-May; face-glands are utilized in marking of territory. French abstract: Les auteurs définissent les caryotypes de deux espèces néarctiques de marmottes, Marmota olympus (2n=40) et M. vancouverensis (2n=42), et donnent des précisions sur les caryotypes de M. flaviventris, M. monax ochracea et M. marmota. Les six especes de Marmota (NF=66) d'Amérique du Nord forment un groupe distinct des espèces d'Asie centrale (NF=70) dont Ie caryotype est connu. Les données caryologiques et les preuves zoogéographiques basées sur la répartition de deux espèces néarctiques de cestodes spécifiques de I'hôte démontrent que M. broweri, dans l'Alaska septentrional, est une relicte du pré-Würm. Elle semble avoir plus d'affinités avec Ie groupe nord américain de caligata qu'avec M. camtschatica du nord de la Sibérie. La présence sur M. broweri de la puce asiatique, Oropsylla silantiewi, n'est pas expliquée. Quelques caractéristiques écologiques et éthologiques de M. broweri sont décrites brièvement et comparées avec celles d'autres espèces. Les groupes familiaux de M. broweri hibernent dans un meme terrier dont l'entrée est bouchée; la copulation à lieu avant que les animaux sortent de leur abri hivernal, it la mi-mai ; ils se servent de leurs glandes faciales pour marquer leur territoire.

Parasites in the neighbourhood: Interactions of the mistletoe Phoradendron affine (Viscaceae) with its dispersers and hosts in urban areas of Brazil

Mistletoes constitute an important food resource for animals in many ecosystems. However, these plants are considered pests in urban areas because of deleterious effects they have on the host trees. Studies in urban areas were mostly focused on listing host species or procedures to control the "pest". In this sense, broader studies including several aspects of mistletoes ecology in urban ecosystems are still missing. We studied the interaction of the mistletoe, Phoradendron affine, with its dispersers and hosts in two urban sites in Uberlandia, Brazil. Phoradendron affine fruits were consumed almost exclusively by Euphonia chlorotica, which was crucial for seed germination. Parasitism was recorded in five hosts, two native (Handroanthus chrysotrichus and Tabebuia roseoalba) and three exotic species (Spathodea campanulata, Ligustrum lucidum and Melia azedarach). Mistletoes were found parasitizing larger host trees, a trend commonly reported for mistletoe-host interaction. Mistletoe seed germination was not affected by the trees species, whether host or non-host, but the radicle of germinated seeds could not penetrate the bark and seedlings invariably died in non-host species. We found a high prevalence of parasitism in our study, in comparison to what previous studies reported for natural areas. The spatial distribution of the hosts and high light incidence on isolated host trees may lead to this high prevalence in urban areas. Rather than eradicated, mistletoes in urban areas should be ecologically managed and their importance for bird species conservation must be considered. More studies to determine which bird species are favoured by mistletoe presence in urban areas will be essential for, this purpose. (C) 2012 Elsevier GmbH. All rights reserved.

Conservação de sementes de ipê-roxo

O objetivo desta pesquisa foi estudar o comportamento fisiológico de sementes de ipê-roxo (Tabebuia heptaphylla (Vell.) Toledo) durante o amazenamento. Frutos colhidos manualmente de 15 plantas-matrizes foram colocados em ambiente sombreado para secagem complementar e posterior extração manual das sementes; primeiro, determinou-se o grau de umidade inicial das sementes (15,6%) e, em seguida, as sementes remanescentes foram submetidas a secagem para obtenção dos demais graus de umidade desejados (11,5, 8,0 e 4,3%). As amostras correspondentes aos diferentes graus de umidade foram armazenadas em câmaras, na temperaturas de 10, 20 e -196 °C. No início e após 120, 240 e 360 dias de armazenamento, as sementes foram submetidas a diversas avaliações fisiológicas. O delineamento experimental foi o inteiramente casualizado, em esquema fatorial, com 4 tratamentos (grau de umidade) no início do armazenamento e 12 tratamentos (4 grau de umidade x 3 condições térmicas), em cada época de avaliação, durante o armazenamento. A comparação das médias foi realizada pelo Teste de Tukey a 5%. A conservação das sementes de ipê-roxo com teores de água de 15,6, 11,5, 8,1 e 4,3% é favorecida no armazenamento a 10 e -196 °C.

Infection at titanium implants with or without clinical inflammation

Objectives: The aims of the present study were (1)to assess the microbiota at implants in function diagnosed as having either peri-implantitis, or mucositis, or being clinically without symptoms of inflammation, (2) to identify explanatory factors to implant status. Material and Methods: Clinical and microbiological data were collected from 138 subjects (mean age: 62.3 ± 14.9) with 524 implants in function for an average of 10.8 years (S.D. +1.5). The checkerboard DNA-DNA hybridization method was used to identify 40 bacterial species. Results: Subjects had poor oral hygiene with a mean % plaque score 53.2 ± 24.4. In 36% of cases periodontitis was reported as the cause for implant therapy. Mucositis was diagnosed in 61.6% and per-implantitis in 15.9% of all cases. Edentulous subjects had at implants with peri-implantitis significantly higher bacterial loads for Streptococcus sanguis (p<0.01), Fusobacterium nucleatum sp. nucleatum (p<0.02), and Leptothrichia buccalis (p<0.05) than did dentate implant subjects. Dentate subjects had higher bacterial loads of Porphyromonas gingivalis (p<0.02). The levels of Fusobacterium nucleatum sp.vincentii and Capnocytophaga ochracea were explanatory to mucositis. Only a history of periodontitis as cause of tooth loss and smoking were explanatory to peri-implantitis. The microbiota was not affect by supportive care patterns. Conclusions: Presence or absence of teeth partly explains the implant microbiota. A past history of periodontitis and smoking are associated with peri-implantitis. The microbiota at implants with mucositis, or peri-implantitis is similar to that of teeth. Supportive periodontal and implant therapy fails to have an impact on implant microbiota and does not prevent mucositis and peri-implantitis.

Does pregnancy have an impact on the subgingival microbiota?

BACKGROUND: We investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care. METHODS: Bacterial samples from weeks 12, 28, and 36 of pregnancy and at 4 to 6 weeks postpartum were processed for 37 species by checkerboard DNA-DNA hybridization. Clinical periodontal data were collected at week 12 and at 4 to 6 weeks postpartum, and bleeding on probing (BOP) was recorded at sites sampled at the four time points. RESULTS: The mean BOP at week 12 and postpartum was 40.1% +/- 18.2% and 27.4% +/- 12.5%, respectively. The corresponding mean BOP at microbiologic test sites was 15% (week 12) and 21% (postpartum; not statistically significant). Total bacterial counts decreased between week 12 and postpartum (P <0.01). Increased bacterial counts over time were found for Neisseria mucosa (P <0.001). Lower counts (P <0.001) were found for Capnocytophaga ochracea, Capnocytophaga sputigena, Eubacterium saburreum, Fusobacterium nucleatum naviforme, Fusobacterium nucleatum polymorphum, Leptotrichia buccalis, Parvimonas micra (previously Peptostreptococcus micros or Micromonas micros), Prevotella intermedia, Prevotella melaninogenica, Staphylococcus aureus, Streptococcus anginosus, Streptococcus intermedius, Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis, Selenomonas noxia, and Veillonella parvula. No changes occurred between weeks 12 and 28 of pregnancy. Counts of Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans), Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), and Treponema denticola did not change. Counts of P. gingivalis and T. forsythia at week 12 were associated with gingivitis (P <0.001). CONCLUSIONS: Subgingival levels of bacteria associated with periodontitis did not change. P. gingivalis and T. forsythia counts were associated with BOP at week 12. A decrease was found in 17 of 37 species from week 12 to postpartum. Only counts of N. mucosa increased.

The impact of the stone age diet on gingival conditions in the absence of oral hygiene

BACKGROUND: The objective of this study was to assess the oral microbiota and clinical data in subjects without access to traditional oral hygiene methods and who ate a diet available in the Stone Age. METHODS: Ten subjects living in an environment replicating the Stone Age for 4 weeks were enrolled in this study. Bleeding on probing (BOP), gingival and plaque indices, and probing depth (PD) were assessed at baseline and at 4 weeks. Microbiologic samples were collected at the mesio-buccal subgingival aspects of all teeth and from the dorsum of the tongue and were processed by checkerboard DNA-DNA hybridization methods. RESULTS: No subject had periodontitis. Mean BOP decreased from 34.8% to 12.6% (P <0.001). Mean gingival index scores changed from 0.38 to 0.43 (not statistically significant) and mean plaque scores increased from 0.68 to 1.47 (P <0.001). PD at sites of subgingival sampling decreased (mean difference: 0.2 mm; P <0.001). At week 4, the total bacterial count was higher (P <0.001) for 24 of 74 species, including Bacteroides ureolyticus, Eikenella corrodens, Lactobacillus acidophilus, Capnocytophaga ochracea, Escherichia coli, Fusobacterium nucleatum naviforme, Haemophilus influenzae, Helicobacter pylori, Porphyromonas endodontalis, Staphylococcus aureus (two strains), Streptococcus agalactiae, Streptococcus anginosis, and Streptococcus mitis. Bacterial counts from tongue samples were higher at baseline (P <0.001) for 20 species, including Tannerella forsythia (previously T. forsythensis), Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans; serotype a), and Streptococcus spp. CONCLUSIONS: The experimental gingivitis protocol is not applicable if the diet (e.g., Stone Age) does not include refined sugars. Although plaque levels increased, BOP and PD decreased. Subgingival bacterial counts increased for several species not linked to periodontitis, whereas tongue bacterial samples decreased during the study period.

Aluminum toxicity to tropical montane forest tree seedlings in southern Ecuador: response of biomass and plant morphology to elevated Al concentrations

In acid tropical forest soils (pH < 5.5) increased mobility of aluminum might limit aboveground productivity. Therefore, we evaluated Al phytotoxicity of three native tree species of tropical montane forests in southern Ecuador. An hydroponic dose-response experiment was conducted. Seedlings of Cedrela odorata L., Heliocarpus americanus L., and Tabebuia chrysantha (Jacq.) G. Nicholson were treated with 0, 300, 600, 1200, and 2400 mu M Al and an organic layer leachate. Dose-response curves were generated for root and shoot morphologic properties to determine effective concentrations (EC). Shoot biomass and healthy leaf area decreased by 44 % to 83 % at 2400 mu M Al, root biomass did not respond (C. odorata), declined by 51 % (H. americanus), or was stimulated at low Al concentrations of 300 mu M (T. chrysantha). EC10 (i.e. reduction by 10 %) values of Al for total biomass were 315 mu M (C. odorata), 219 mu M (H. americanus), and 368 mu M (T. chrysantha). Helicarpus americanus, a fast growing pioneer tree species, was most sensitive to Al toxicity. Negative effects were strongest if plants grew in organic layer leachate, indicating limitation of plant growth by nutrient scarcity rather than Al toxicity. Al toxicity occurred at Al concentrations far above those in native organic layer leachate.