Avaliação microbiológica da superfície dos implantes ortopédicos metálicos usados para fixar fraturas ósseas

In the majority of cases of bone fracture requiring surgery, orthopedic implants (screw-plate and screw) are used for osteosynthesis and the infections associated with such implants are due to the growth of microorganisms in biofilms. The objective of this study was to identify microorganisms recovered from osteosynthesis implants used to fix bone fractures, to assess the viability of the cells and the ability of staphylococci to adhere to a substrate and to determine their sensitivity/resistance to antimicrobials. After surgical removal, the metal parts of austenitic stainless steel (ASTM F138/F139 or ISO NBR 5832-1/9) were transported to the Laboratory of Clinical Microbiology, washed in buffer and subjected to ultrasonic bath at 40±2 kHz for 5 minutes. The sonicated fluid was used to seed solid culture media and cell viability was assessed under the microscope by with the aid of a fluorescent marker. The production of extracellular polysaccharide by Staphylococcus spp. was investigated by means of adhesion to a polystyrene plate. The profile of susceptibility to antimicrobials was determined by the disk diffusion assay. The most frequently isolated bacteria included coagulase-negative Staphylococcus resistant to erythromycin, clindamycin and oxacillin. Less frequent were Pseudomonas aeruginosa resistant to trimethoprim/sulfamethoxazole and ampicillin, Acinetobacter baumannii resistant to ceftazidime, Enterobacter cloacae resistant to cephalothin, cefoxitin, cefazolin, levofloxacin and ciprofloxacin, Bacillus spp. and Candida tropicalis. The observation of slides by fluorescence microscope showed clusters of living cells embedded in a transparent matrix. The test for adherence of coagulase-negative Staphylococcus to a polystyrene plate showed that these microorganisms produce extracellular polysaccharide. In conclusion, the metal parts were colonized by bacteria related to orthopedic implant infection, which were resistant to multiple antibiotics.

Avaliação da liberação de íon fluoreto de cimentos ionoméricos antes e após a recarga e com proteção da superfície

Objective: To evaluate fluoride ion release from two anhydrous glass ionomer cements (GICs) and two resin-modified GICs (RMGICs) before and after recharge with 2% neutral sodium fluoride for 4 min and after surface protection of the Maxxion R GIC with an adhesive system, a cavity varnish and a colorless nail polish. Method: A stainless steel 2x6 mm matrix was used for fabricating 5 specimens of each material, which were immersed in 5 mL of deionized water, renewed every 24 h. Measurements with a potentiometer were performed on days 1, 2, 9 and 17, in the 1st and 2nd phases, and the specimens were buffered with a TISAB III solution. In the 2nd phase, the specimens were subjected to recharge and immersed again in 5 mL of deionized water. In the 3rd phase, the GIC surfaces were protected and readings were made at 5 min, 24 h, 48 h and 72 h. Tukey's post-hoc and Student's t tests were used for statistical analyses (p<0.05). Results: There was statistically significant difference in the comparison between the 1st and 2nd phases for all materials, except at day 2 for Vidrion R and VitroFil LC. In the 3rd phase, it was observed that for all materials, comparison of the first 5 min with the other times revealed statistically significant differences among the means of fluoride ion release. In the comparison with the other times, both the varnish and the colorless nail polish presented statistically significant difference between 24 and 48 h as well as between 24 and 72 h. Conclusion: The anhydrous GICs were more effective in fluoride ion release and recharge compared with the RMGICs. Maxxion R presented a homogeneous and statistically significant behavior in both phases. All materials for surface protection were efficient and the colorless nail polish had the best behavior.

Obtenção de fosfatos de cálcio pelo método biomimético sobre a superfície da liga Ti-6Al-4V modificada pelo laser Nd:YAG

In this work, surface modification of the Ti-6Al-4V alloy by irradiation with a Nd:YAG laser beam was studied. The matrix spacing was varied and the other parameters were kept constant. After theirradiation, the samples were immersed into SBF solution (Simulated Body Fluid) for 7 days at 37 ° C for nucleation and formation of calcium phosphates. After this period, the samples were subjected to a heat treatment at 600 °C. X-ray diffraction results indicated the formation of a mixture of phases containing hydroxyapatite and tricalcium phosphate. Infrared analysis demonstrated the presence of bands related to hydroxyapatite. SEM micrographs showed that different matrix spacing used leads to the formation of different morphologies.The 0.01 cm spacing induced the formation of microspheres, characteristic of the hydroxyapatite phase, while for 0.02 cm spacing, only a non-homogeneous coating was obtained. Therefore, the use of laser in the production of active surfaces for deposition of bioceramics proved to be viable, and the final coating has potential for applications in the field of dental implants.

Detecção de Crateras de Impacto em Imagens Digitais. Estudo de caso: Superfície de Marte

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O emprego da metodologia de superfície de resposta no desenvolvimento de um novo produto simbiótico, fermentado com Enterococcus faecium CRL 183 e Lactobacillus helveticus ssp jugurti 416, à base de extratos aquosos de soja e de Yacon (Smallanthus sonchifolius)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo experimental do escoamento em cavidades abertas utilizando um canal de superfície livre

Pós-graduação em Engenharia Mecânica - FEIS

Abordagem matemática de roll waves em escoamentos hiperconcentrados com superfície livre

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Um panorama sobre roll waves em escoamentos laminares e turbulentos com superfície livre

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reação tecidual à superfície de titânio tratada pela irradiação laser e hidroxiapatita: análise histomorfométrica em ratos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Reimplante dentário tardio após o tratamento da superfície radicular com hipoclorito de sódio e fluoreto de sódio: análise histomorfométrica em ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do tratamento da superfície radicular com própole ou com flúor no reimplante tardio de dentes de ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo in vitro das alterações na superfície do titânio comercialmente puro e da liga de titânio Ti -6Al-4V submetida a diferentes tratamentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do tratamento de superfície na adaptação externa de cavidade classe V hígida e afetada por cárie submetidas á ciclagem térmica

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito de substâncias ácidas na rugosidade de superfície e dureza knoop de materiais restauradores: estudo in situ

Pós-graduação em Odontologia - FOA

Influência do selante de superfície em propriedades físicas e mecânicas de resinas compostas submetidas a envelhecimentos artificiais

Pós-graduação em Odontologia - FOA