Structural characterization of novel chemotactic and mastoparan peptides from the venom of the social wasp Agelaia pallipes pallipes by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Facing Hymenoptera venom allergy: from natural to recombinant allergens

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Síntese e caracterização de pequenos peptídeos lineares do veneno de Tityus serrulatus (Buthidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Tropidomyrmex elianae, a new myrmicine ant genus and species from Brazil, tentatively assigned to Solenopsidini (Hymenoptera, Formicidae)

A new myrmicine ant, Tropidomyrmex elianae gen. n. & sp. n., is described from southeastern and central Brazil, based on workers, ergatoid gynes, males and larvae. Tropidomyrmex workers are relatively small, monomorphic, characterized mainly by the feebly pigmented and extremely thin integument; subfalcate mandibles bearing a single apical tooth; palpal formula 1,2; clypeus relatively broad and convex; reduced compound eyes; propodeum unarmed and with a strongly medially depressed declivous face; double and bilobed well developed subpostpetiolar processes; and peculiarities in the sting apparatus. A colony fragment of T. elianae containing workers, ergatoid gynes, males, and brood was found inside a ground termite nest (Anoplotermes pacificus Apicotermitinae) in a montane rocky scrubland in the state of Minas Gerais, southeastern Brazil. Tropidomyrmex elianae is known also from two workers collected in leaf litter samples processed with a Winkler extractor, from the state of Tocantins, central-north Brazil. Despite the differences from the accepted solenopsidine genera, Tropidomyrmex is tentatively assigned to this tribe. Within the solenopsidine ants, the genus is apparently related to Tranopelta. Tropidomyrmex is marked by extreme reductions, perhaps reflecting adaptations to particular habits and habitats.

Revisionary studies on the enigmatic neotropical ant genus Stegomyrmex Emery, 1912 (Hymenoptera : Formicidae : Myrmicinae), with the description of two new species

The recent increase in leaf litter ants sampling effort in Neotropical wet forests has revealed new and interesting records of the highly specialized myrmicine ant genus Stegomyrmex Emery, previously considered as extremely rare. We present a modified diagnosis for the genus and describe Stegomyrmex bensoni n. sp. and S. olindae n. sp., based on, respectively, workers, males, and gyne (central-north Brazil) and on a single worker (northern Brazil). Stegomyrmex vizottoi Diniz (southeastern Brazil) is redescribed and compared with S. olindae n. sp.; these species present significant differences in size, sculpturation, and sting apparatus morphology. The males of S. vizottoi are described for the first time. A key for workers and queens and a distribution map for the five know Stegomyrmex species are provided.

PLGA microspheres containing bee venom proteins for preventive immunotherapy

Bee venom (BV) allergy is potentially dangerous for allergic individuals because a single bee sting may induce an anaphylactic reaction, eventually leading to death. Currently, venom immunotherapy (VIT) is the only treatment with long-lasting effect for this kind of allergy and its efficiency has been recognized worldwide. This therapy consists of subcutaneous injections of gradually increasing doses of the allergen. This causes patient lack of compliance due to a long time of treatment with a total of 30-80 injections administered over years. In this article we deal with the characterization of different MS-PLGA formulations containing BV proteins for VIT. The PLGA microspheres containing BV represent a strategy to replace the multiple injections, because they can control the solute release. Physical and biochemical methods were used to analyze and characterize their preparation. Microspheres with encapsulation efficiencies of 49-75% were obtained with a BV triphasic release profile. Among them, the MS-PLGA 34 kDa-COOH showed to be best for VIT because they presented a low initial burst (20%) and a slow BV release during lag phase. Furthermore, few conformational changes were observed in the released BV. Above all, the BV remained immunologically recognizable, which means that they could continuously stimulate the immune system. Those microspheres containing BV could replace sequential injections of traditional VIT with the remarkable advantage of reduced number of injections. (C) 2011 Elsevier B.V. All rights reserved.

Influence of total and specific IgE, serum tryptase, and age on severity of allergic reactions to Hymenoptera stings

The aim of this study was to analyze the influence of total serum IgE and other potential risk factors on severity of systemic allergic Hymenoptera sting reactions.

Use of an indirect enzyme-linked immunosorbent assay for detection of antibodies in sheep naturally infected with Salmonella Abortusovis

An indirect enzyme-linked immunosorbent assay (ELISA) was modified and validated to detect antibodies against Salmonella Abortusovis in naturally infected sheep. The ELISA was validated with 44 positive and 45 negative control serum samples. Compared with the immunoblot, the sensitivity and specificity of the assay were 98% and 100%, respectively. To follow antibody levels over time, samples from 12 infected ewes were collected at 1, 3, and 10 months after abortion. All animals showed antibody levels above the cutoff value throughout the observation period. One and 3 months after abortion, high antibody levels could be detected in all but one animal, whereas after 10 months, 9 animals had markedly lower but still positive antibody levels. The test characteristics and evidence for the persistence of detectable antibody levels in all infected animals for up to 10 months indicates that the ELISA can be used for herd surveillance testing.

Clinical and immunologic effects of H1 antihistamine preventive medication during honeybee venom immunotherapy

BACKGROUND: H1 antihistamines increase safety during allergen-specific immunotherapy and might influence the outcome because of immunoregulatory effects. OBJECTIVE: We sought to analyze the influence of 5 mg of levocetirizine (LC) on the safety, efficacy, and immunologic effects of ultrarush honeybee venom immunotherapy (BVIT). METHOD: In a double-blind, placebo-controlled study 54 patients with honeybee venom allergy received LC or placebo from 2 days before BVIT to day 21. Side effects during dose increase and systemic allergic reactions (SARs) to a sting challenge after 120 days were analyzed. Allergen-specific immune response was investigated in skin, serum, and allergen-stimulated T-cell cultures. RESULTS: Side effects were significantly more frequent in patients receiving placebo. Four patients receiving placebo dropped out because of side effects. SARs to the sting challenge occurred in 8 patients (6 in the LC group and 2 in the placebo group). Seven SARs were only cutaneous, and 1 in the placebo group was also respiratory. Difference of SARs caused by the sting challenge was insignificant. Specific IgG levels increased significantly in both groups. Major allergen phospholipase A(2)-stimulated T cells from both groups showed a slightly decreased proliferation. The decrease in IFN-gamma and IL-13 levels with placebo was not prominent with LC, whereas IL-10 levels showed a significant increase in the LC group only. Decreased histamine receptor (HR)1/HR2 ratio in allergen-specific T cells on day 21 in the placebo group was prevented by LC. CONCLUSIONS: LC reduces side effects during dose increase without influencing the efficacy of BVIT. LC modulates the natural course of allergen-specific immune response and affects the expression of HRs and cytokine production by allergen-specific T cells.

Soziale Dienste, Schule und zivilgesellschaftliches Klima

In an international perspective cooperation between social services and school has a long tradition. In the German speaking countries we can recognize a historical distance or gap between school and “social pedagogy”, but despite this tradition new forms of cooperation are arising since the last few years. This tendency is part of the development of European societies into “knowledge-based societies” where knowledge and cultural capital are becoming ever stricter criteria for participation in society. This puts particular pressure on those adolescents who threaten to fail in the positional competition for educational qualifications. And it tends to the reproduction and reinforcing of social inequalities due to inequalities in education. For that reason in the article the development of school related social services in different European countries is investigated and it is shown that the increasing pressure to qualification and selection in school creates various problems of integration. Social dimensions of education are pointed out delivering starting points for the cooperation of social services in school and opening opportunities for productive forms of coping with differences between family background, informal social environment and educational milieu in school. Particular attention is paid to differences in socio cultural habits, in socio economical opportunities and in collective practices of interaction. A central focus in the contribution is the orientation towards a participative civil society climate relevant for the interaction between teachers and pupils and between professionals and addressees of social services as well. It is a task of future research in school related social services to analyse their institutional structure and their practices of professional interaction and to find out by European and international comparison in which way social services can contribute to the establishing of a participative civil society climate in school.

Organisational and methodical aspects of drug and addiction prevention among young people. Developments in the Ukraine, in Germany, Spain and Russia (Project Description)

From october 2000 to march 2003 the project has been coordinated by the institute of social work and welfare studies of the TU Dresden and financed by the European Commission (INCO-COPERNICUS-Programme). It was an international cooperation between the TU Dresden (Germany), the University of Internal Affairs and the National Technical University in Kharkiv (Ukraine), the research institute AREA in Valenciá (Spain) and the Belgorod Juridical Institute of the Ministry of Internal Affairs (Russia) which was established for improving the knowledge base of drug prevention activities by the East European partners. The overall aim of the project was the analysis and elaboration of approaches to drug and addiction prevention. Under consideration of the specific conditions within the East-European countries this work should contribute to the development of pluralistic approaches towards health promotion which are embedded into an intercultural, European horizon.

Canciones que cuentan historias

La música en la clase de inglés ha tenido siempre un rol muy importante. Estudios sobre el uso de la música en estas clases han demostrado que escuchar canciones en el aula resulta una experiencia agradable que permite a los alumnos adquirir la lengua extranjera de un modo placentero y significativo. Mediante las canciones podemos exponer a los alumnos a usos reales de la lengua extranjera, donde vocabulario y estructuras gramaticales cobran sentido al estar relacionadas a un determinado contexto de uso. Las historias que cuentan las canciones lograrán cautivar aún más la atención de nuestros alumnos que si solo los exponemos a los textos frecuentemente usados en la clase de ILE. En este trabajo presentaremos una metodología práctica para enseñar y explotar los elementos lingüístico-literarios, socio-culturales y musicales de las canciones. Esto lo ejemplificaremos a través de una secuencia didáctica utilizando la canción "Moon over Bourbon Street" del cantante y compositor Sting

Canciones que cuentan historias

La música en la clase de inglés ha tenido siempre un rol muy importante. Estudios sobre el uso de la música en estas clases han demostrado que escuchar canciones en el aula resulta una experiencia agradable que permite a los alumnos adquirir la lengua extranjera de un modo placentero y significativo. Mediante las canciones podemos exponer a los alumnos a usos reales de la lengua extranjera, donde vocabulario y estructuras gramaticales cobran sentido al estar relacionadas a un determinado contexto de uso. Las historias que cuentan las canciones lograrán cautivar aún más la atención de nuestros alumnos que si solo los exponemos a los textos frecuentemente usados en la clase de ILE. En este trabajo presentaremos una metodología práctica para enseñar y explotar los elementos lingüístico-literarios, socio-culturales y musicales de las canciones. Esto lo ejemplificaremos a través de una secuencia didáctica utilizando la canción "Moon over Bourbon Street" del cantante y compositor Sting

Canciones que cuentan historias

La música en la clase de inglés ha tenido siempre un rol muy importante. Estudios sobre el uso de la música en estas clases han demostrado que escuchar canciones en el aula resulta una experiencia agradable que permite a los alumnos adquirir la lengua extranjera de un modo placentero y significativo. Mediante las canciones podemos exponer a los alumnos a usos reales de la lengua extranjera, donde vocabulario y estructuras gramaticales cobran sentido al estar relacionadas a un determinado contexto de uso. Las historias que cuentan las canciones lograrán cautivar aún más la atención de nuestros alumnos que si solo los exponemos a los textos frecuentemente usados en la clase de ILE. En este trabajo presentaremos una metodología práctica para enseñar y explotar los elementos lingüístico-literarios, socio-culturales y musicales de las canciones. Esto lo ejemplificaremos a través de una secuencia didáctica utilizando la canción "Moon over Bourbon Street" del cantante y compositor Sting

...Un-natural history, or Myths of ancient science; being a collection of curious tracts on the basilisk, unicorn, phoenix, behemoth or leviathan, dragon, giant spider, tarantula, chameleons, satyrs, homines caudati, &e.,

"This edition is limited to 275 small paper copies, and 75 large paper copies."