Studies on the toxicological effects of Asulox-40 and Emisan-6 to eggs and early life history stages of Sarotherodon mossambicus

Toxicological effects of Asulox-40 and Emisan-6 to eggs and early life history stages of Sarotherodon mossambicus were reported. 80% of egg hatching occurred in the controls, 1 p.p.m and 5 p.p.m concentrations of Asulox-40. 10 p.p.m. and 50 p.p.m. concentrations of the same toxicants had 70% and 60% hatchings while in Emisan-6 in the same concentrations the hatching were 70% and! 40%. In 100 p.p.m. concentration of both toxicants 20% incomplete hatching occurred. In Emisan-6 Lc 50 and Lc 100 values were recorded at 32 hand 96h respectively in 10 p.p.m. concentrations. In Asulox-40 the same values were recorded in 24h and 40h respectively at 50 p.p.m. concentration. The fish activity during the experimental period showed initial hyper activity. It was established that the Emisan-6 is more harmful to S. mossambicus than Asulox-40. The harmless concentrations of these chemicals were 1.2 p.p.m. for Asulox-40 and 0.6 p.p.m. for Emisan-6.

Survival rates of different postlarval stages of Penaeus monodon Fabricius

The objective of this study is to determine survival rates of different postlarval stages upon stocking in the Leganes ponds. Twelve 3m x 2m x 2m suspension nets made of nylon cloth (mesh size = 0 . 1 mm) were set up in a Leganes Station pond (ave. water depth = 1 m) by means of 3-m long poles stacked at distances approximating the area of each net. The net bottom was filled with topsoil at least 15 cm thick to stimulate the pond bottom. At least 60 cm of the upper edge of each net was above the water level to prevent mixing of water inside and outside the net. P.monodon of stages P SUB-11 , P SUB-15 , P SUB-21 (from the hatchery) and P SUB-25 (from the wet lab) were stocked in the nets at 200/sq m or 1,200 fry/net. Due to lack of fry, only one P SUB-25 net was stocked. Each net had two large dried miapi branches as shelter from predation and cannibalism for the young sugpo fry. Fresh lablab was fed at the rate of one pail (approximately 5 kg) every four days per net. Harvest data show relatively higher survival rates for P SUB-15 and P SUB-18 compared to P SUB-11 and P SUB-25 with no significant difference between these two stages. The results for P SUB-25 may not be valid because the stock came from the wet lab in comparison to the other postlarval stages which were reared in the hatchery. Moreover, the P SUB-25 stock had no replicates and the net itself (no. 10) was discovered to have many holes. These preliminary results point to P SUB-15 as the best stage for harvest from the hatchery in terms of high pond recovery and lesser expense in rearing compared to older postlarvae.

Determination of acute mercury toxicity to developing stages of Cyprinus carpio and Cirrhinus mrigala

Toxicity of inorganic mercury to different life history stages of fresh water fishes, Cyprinus carpio and Cirrhinus mrigala were demonstrated by static bioassays. 48 and 94% of egg hatching occurred in controls at 72 and 24h of experimentation in C. carpio and C. mrigala respectively. While fish eggs in water containing mercuric chloride showed delayed development as compared to the control. LC50, LC100 and safe concentrations of hatchling, fry and fingerling were calculated. Hatchling and fry were observed to be more susceptible as compared to fingerlings of C. carpio and C. mrigala.

Relationship between diet composition and growth rate of the zoeal and mysis stages of Penaeus japonicus Bate

Diets containing Chaetoceros gracilis plus Artemia nauplii artificially prepared diet, Diet-B, and two commercial feeds Tapes and mysid meals, were fed to larvae of P. japonicus. Highest survival rate was obtained when larvae were fed with Diet-B. The results show that the early larval stages of P. japonicus can be reared on artificially prepared diets. Since the chemical composition of the diet is known, it can be used as supplemental data for larval feeding development and nutritional requirement studies for the early larval stages of Penaeus japonicus and/or other penaeids. Information is tabulated on feeds and feeding rates used, composition of the artificial diet, fatty acid composition of lipids of the different diets, and of the sterols of the different diets.

Preliminary report on the first production of the crab stages of alimasag, Portunus pelagicus (Linnaeus)

An experiment was undertaken studying the early life history of Portunus pelagicus in order to obtain information of relevance to rearing techniques for crabs.

Notes on the pigmentation pattern in the larval developmental stages of laboratory-reared milkfish

Milkfish fry were artificially bred and reared in the laboratory and the pigmentation pattern of the different developmental stages of the larvae are described in detail, with illustrations.

Effects of atrazine herbicide on physiological, biochemical and histological biomarkers of developmental stages of larvae and fry in Caspian kutum, Rutilus frisii kutum

To investigation of the toxic effects of atrazine on newly hatched larvae and releasing age fry of the Caspian Kutum, Rutilus frisii kutum, the 96h LC50 was determined as 18.53 ppm and 24.95 ppm, respectively. Newly hatched larvae were exposed to three sublethal concentrations of atrazine (1/2LC50, 1/4LC50 and 1/8LC50) for 7 days. Different histopathological alterations were observed in fins and integument, gills, Kidney, digestive system, liver and the brain of the exposed larvae. Fry’s were exposed to one sublethal concentration of atrazine (1/2LC50) for four days, and like the larvae’s, many histopathological alterations were observed in fins and integument, gills, Kidney, digestive system, liver and the brain of the exposed fry’s, too. Also, measurements of the body ions: Na+, K+, Ca2+, Mg2+ and Cl- in atrazine exposed larvae and fry’s compare to control groups showed that atrazine is changed the body ions composition. No significant differences were found in length growth rate, weight growth rate and the condition factor of the atrazine exposed larvae and fry. Immunohistochemical localization of the Na+, K+-ATPase in integumentary and gill ionocytes, showed no differences in dispersion pattern of the ionocytes in atrazine exposed larvae and fry, compare to control group. Measuring the dimensions of the ionocytes and counting the ionocytes showed that atrazine is affecting on ionocytes by mild increasing in size and mild decreasing in number. Ultrastructural studies, using SEM and TEM, showed that atrazine have significant effects on cellular and subcellular properties. It caused necrosis in surface of the pavement cells in branchial epithelium, necrosis in endoplasmic reticulum of the ionocytes and changed the shape of the mitochondria in these cells. Results showed that sublethal concentrations of atrazine were very toxic to larvae and fry of the Rutilus frisii kutum, and at these levels can made some serious histopathological alterations in their tissues. Related to the severe histopathological alterations in osmoregulatory organs, like gill, kidney and digestive system, and the alterations in the body ion composition, it could be concluded that atrazine could interfere with the osmoregulation process of the Rutilus frisii kutum at the early stages of the life history.

Observation of early to full covering stages of ethylene-based CVD of graphene

Scalable growth is essential for graphene-based applications. Recent development has enabled the achievement of the scalability by use of chemical vapor deposition (CVD) at 1000°C with copper as a catalyst and methane as a precursor gas. Here we report our observation of early stage of graphene growth based on an ethylene-based CVD method, capable of reducing the growth temperature to 770°C for monolayer graphene growth on copper. We track the early stages of slow growth under low ethylene flow rate and observe the graphene domain evolution by varying the temperature and growth time. Temperature-dependence of graphene domain density gives an apparent activation energy of 1.0 eV for nucleation.