Garat, 1762-1823 / Paul Lafond.

Fecha de publicación tomada de: Eusko bibliographia. v. IV, p. 567

Joannis-Emmanuel Gilibert, olim in Collegio ... Exercitia phytologica quibus omnes plantae europaeae [sic], quas vivas invenit in variis herbationibus, seu in Lithuania, Gallia ... : [Texto impreso] volumen primum : plantae lithuanicae cum lugdunensibus comparatae

Tit. en antep.: "Supplementum systematis plantarum Europae"

Joannis-Emmanuel Gilibert, olim in Collegio ... Exercitia phytologica quibus omnes plantae europeae, quas vivas invenit in variis herbationibus, seu in Lithuania, Gallia ... : [Texto impreso] volumen secundum : caeterae plantae lithuanicae cum lugdunensibus comparatae

Tit. en antep.: "Supplementum systematis plantarum Europae"

A method for soluble overexpression of the α7 nicotinic acetylcholine receptor extracellular domain

We describe the construction of a soluble protein carrying the N-terminal extracellular domain (ECD) of the α7 subunit of the nicotinic acetylcholine receptor. The approach was to fuse the α7 ECD at the C and N termini of several monomeric and pentameric soluble carrier proteins and to investigate the soluble expression of the product in Escherichia coli. An initial screening of six carrier proteins resulted in the selection of a fusion protein comprising, from the N to the C terminus, the maltose binding protein, a 17-aa linker containing an enterokinase binding site, and the α7 ECD. This protein is soluble upon expression in bacteria and is purified by affinity chromatography. It binds the competitive nicotinic antagonist α-bungarotoxin with 2.5 μM affinity and displays a CD spectrum corresponding to a folded protein. The method might be suitable to produce large quantities of protein for crystallization and immunochemical experiments.

Evidence for a Slow-Turnover Form of the Ca2+-Independent Phosphoenolpyruvate Carboxylase Kinase in the Aleurone-Endosperm Tissue of Germinating Barley Seeds1

Phosphoenolpyruvate carboxylase (PEPC) activity was detected in aleurone-endosperm extracts of barley (Hordeum vulgare) seeds during germination, and specific anti-sorghum (Sorghum bicolor) C4 PEPC polyclonal antibodies immunodecorated constitutive 103-kD and inducible 108-kD PEPC polypeptides in western analysis. The 103- and 108-kD polypeptides were radiolabeled in situ after imbibition for up to 1.5 d in 32P-labeled inorganic phosphate. In vitro phosphorylation by a Ca2+-independent PEPC protein kinase (PK) in crude extracts enhanced the enzyme's velocity and decreased its sensitivity to l-malate at suboptimal pH and [PEP]. Isolated aleurone cell protoplasts contained both phosphorylated PEPC and a Ca2+-independent PEPC-PK that was partially purified by affinity chromatography on blue dextran-agarose. This PK activity was present in dry seeds, and PEPC phosphorylation in situ during imbibition was not affected by the cytosolic protein-synthesis inhibitor cycloheximide, by weak acids, or by various pharmacological reagents that had proven to be effective blockers of the light signal transduction chain and PEPC phosphorylation in C4 mesophyll protoplasts. These collective data support the hypothesis that this Ca2+-independent PEPC-PK was formed during maturation of barley seeds and that its presumed underlying signaling elements were no longer operative during germination.

Le Pausanias français : état des arts du dessin en France, à l'ouverture du XIXe siecle : Salon de 1806 : ouvrage dans lequel les principales productions de l'école actuelle sont classées, expliquées, analysées, à l'aide d'un commentaire exact, raisonné, et représentées dans une suite de dessins exécutés et gravés par les plus habiles artistes : on y a joint quelques portraits, gravés au trait, de grands artistes vivans, avec des notices historiques et inédites, concernant leur personne et leurs ouvrages /

Barbier, A.A. Ouvrages anonymes,

Œuvres complètes /

Includes indexes.

Observations sur les affections catarrhales en général, et paticulièrement sur celles connues sous les noms de rhumes de cerveau et de rhumes de poitrine /

Mode of access: Internet.

Observaciones sobre los efectos catarrales en general, y particularmente sobre los que se llaman reumas ó fluxiones de cerebro y de pecho /

Mode of access: Internet.

Orontii Finei ... Protomathesis ...

Mode of access: Internet.

Traité du choix et de la methode des etudes /

Mode of access: Internet.

Les moeurs des israelites /

Mode of access: Internet.

Nouveau dictionnaire historique ou Histoire abrégée de tous les hommes qui se sont fait un nom par le génie, les talens, les vertus, les erreurs [et]c., depuis le commencement du monde jusqu'à nos jours ... /

Q-Z.

Nouveau dictionnaire historique ou Histoire abrégée de tous les hommes qui se sont fait un nom par le génie, les talens, les vertus, les erreurs [et]c., depuis le commencement du monde jusqu'à nos jours ... /

C-F.

Nouveau dictionnaire historique ou Histoire abrégée de tous les hommes qui se sont fait un nom par le génie, les talens, les vertus, les erreurs même [et]c., depuis le commencement du monde jusqu'à nos jours ... /

G-K.