"Quissamã somos nós!": construção participativa de hipertexto

Trata-se de pesquisa realizada em conjunto com a comunidade de Quissamã-RJ, representada pelos alunos, professores e outros agentes educacionais de seis escolas do município. Objetivou a construção de um hipertexto digital, aqui considerado como parte integrante do regime de informação e estrutura significante [informação] para a produção do conhecimento. Parte do princípio de que, para atingir este fim, é necessário a socialização da informação, como parte da responsabilidade social da ciência da informação. O hipertexto é visto como um instrumento para produzir e organizar informações visando à sua disponibilização na Internet, espaço potencial de socialização da informação. O conteúdo informacional do hipertexto reflete um regime de informação sobre a identidade cultural de Quissamã, que tem no folclore um dos elementos mais expressivo.

Essential residues in the H-NS binding site of Hha, a co-regulator of horizontally acquired genes in Enterobacteria

Proteins of the Hha/YmoA family co-regulate with H-NS the expression of horizontally acquired genes in Enterobacteria. Systematic mutations of conserved acidic residues in Hha have allowed the identification of D48 as an essential residue for H-NS binding and the involvement of E25. Mutations of these residues resulted in deregulation of sensitive genes in vivo. D48 is only partially solvent accessible, yet it defines the functional binding interface between Hha and H-NS confirming that Hha has to undergo a conformational change to bind H-NS. Exposed acidic residues, such as E25, may electrostatically facilitate and direct the approach of Hha to the positively charged region of H-NS enabling the formation of the final complex when D48 becomes accessible by a conformational change of Hha.

Suomen ns. uhrikirkkojen taustaa, historiaa ja tarinaperinnettä

Zusammengassung: Hintergrund, Geschichte und Erzähltradition der sog. Votivkirchen in Finnland

Antiphonarium, ns. Katariina-käsikirjoitus, käsikirjoitusfragmentti - [1]

Katkelma Pyhän Henrikin varhaisaamun rukoushetkestä (matutinum), mm. legendaresitaatioiden kera laulettavia responsoriosävelmiä.

On the origin of the selectivity of plasmidic H-NS towards horizontally acquired DNA: Linking H-NS oligomerization and cooperative DNA binding

The nucleoid-associated protein H-NS is a global modulator of the expression of genes associated with adaptation to environmental changes. A variant of H-NS expressed in the R27 plasmid was previously shown to selectively modulate the expression of horizontally acquired genes, with minimal effects on core genes that are repressed by the chromosomal form of H-NS. Both H-NS proteins are formed by an oligomerization domain and a DNA-binding domain, which are connected by a linker that is highly flexible in the absence of DNA. We studied DNA binding by means of oligomer-forming chimeric proteins in which domains of the chromosomal and plasmidic variants are exchanged, as well as in monomeric truncated forms containing the DNA-binding domain and variable portions of the linker. Point mutations in the linker were also examined in full-length and truncated H-NS constructs. These experiments show that the linker region contributes to DNA binding affinity and that it is a main component of the distinct DNA binding properties of chromosomal and plasmidic H-NS. We propose that interactions between the linker and DNA limit the flexibility of the connection between H- NS oligomerization and DNA binding and provide an allosteric indirect readout mechanism to detect long- range distortions of DNA, thus enabling discrimination between core and horizontally acquired DNA.