Un estudio del adjetivo en un corpus de textos escritos en inglés y español pertenecientes al discurso de especialidad de las Tecnologías de la Información y las Comunicaciones

Esta investigación se enmarca dentro de los denominados lenguajes de especialidad que para esta tesis será el de las Tecnologías de la Información y la Comunicación (TIC). De todos los aspectos relacionados con el estudio de estos lenguajes que pudieran tener interés lingüístico ha primado el análisis del componente terminológico. Tradicionalmente la conceptualización de un campo del saber se representaba mayoritariamente a través del elemento nominal, así lo defiende la Teoría General de la Terminología (Wüster, 1968). Tanto la lexicología como la lexicografía han aportado importantes contribuciones a los estudios terminológicos para la identificación del componente léxico a través del cual se transmite la información especializada. No obstante esos primeros estudios terminológicos que apuntaban al sustantivo como elmentos denominativo-conceptual, otras teorías más recientes, entre las que destacamos la Teoría Comunicativa de la Terminología (Cabré, 1999) identifican otras estructuras morfosintácticas integradas por otros elementos no nominales portadores igualmente de esa carga conceptual. A partir de esta consideración, hemos seleccionado para este estudio el adjetivo relacional en tanto que representa otra categoría gramatical distinta al sustantivo y mantiene un vínculo con éste debido a su procedencia. Todo lo cual puede suscitar cierto interés terminológico. A través de esta investigación, nos hemos propuesto demostrar las siguientes hipótesis: 1. El adjetivo relacional aporta contenido especializado en su asociación con el componente nominal. 2. El adjetivo relacional es portador de un valor semántico que hace posible identificar con más precisión la relación conceptual de los elementos -adjetivo y sustantivo - de la combinación léxica resultante, especialmente en algunas formaciones ambiguas. 3. El adjetivo relacional, como modificador natural del sustantivo al que acompaña, podría imponer cierta restricción en sus combinaciones y, por tanto, hacer una selección discriminada de los integrantes de la combinación léxica especializada. Teniendo en cuenta las anteriores hipótesis, esta investigación ha delimitado y caracterizado el segmento léxico objeto de estudio: la ‘combinación léxica especializada (CLE)’ formalmente representada por la estructura sintáctica [adjR+n], en donde adjR es el adjetivo y n el sustantivo al que acompaña. De igual forma hemos descrito el marco teórico desde el que abordar nuestro análisis. Se trata de la teoría del Lexicón Generatvio (LG) y de la representación semántica (Pustojovsky, 1995) que propone como explicación de la generación de significados. Hemos analizado las distintas estructuras de representación léxica y en especial la estructura qualia a través de la cual hemos identificado la relación semántica que mantienen los dos ítems léxicos [adjR+n] de la estructura sintáctica de nuestro estudio. El estudio semántico de las dos piezas léxicas ha permitido, además, comprobar el valor denominativo del adjetivo en la combinación. Ha sido necesario elaborar un corpus de textos escritos en inglés y español pertenecientes al discurso de especialidad de las TIC. Este material ha sido procesado para nuestros fines utilizando distintas herramientas electrónicas. Se ha hecho uso de lexicones electrónicos, diccionarios online generales y de especialidad y corpus de referencia online, estos últimos para poder eventualmente validad nuetros datos. Asimismo se han utilizado motores de búsqueda, entre ellos WordNet Search 3.1, para obtener la información semántica de nuestros elementos léxicos. Nuestras conclusiones han corroborado las hipótesis que se planteaban en esta tesis, en especial la referente al valor denominativo-conceptual del adjetivo relacional el cual, junto con el sustantivo al que acompaña, forma parte de la representación cognitiva del lenguaje de especialidad de las TIC. Como continuación a este estudio se proponen sugerencias sobre líneas futuras de investigación así como el diseño de herramientas informáticas que pudieran incorporar estos datos semánticos como complemento de los ítems léxicos dotados de valor denominativo-conceptual. ABSTRACT This research falls within the field of the so-called Specialized Languages which for the purpose of this study is the Information and Communication Technology (ICT) discourse. Considering their several distinguishing features terminology concentrates our interest from the point of view of linguistics. It is broadly assumed that terms represent concepts of a subject field. For the classical view of terminology (Wüster, 1968) these terms are formally represented by nouns. Both lexicology and terminology have made significant contributions to the study of terms. Later research as well as other theories on Terminology such as the Communicative Theory of Terminology (Cabré, 1993) have shown that other lexical units can also represent knowledge organization. On these bases, we have focused our research on the relational adjective which represents a functional unit different from a noun while still connected to the noun by means of its nominal root. This may have a potential terminological interest. Therefore the present research is based on the next hypotheses: 1. The relational adjective conveys specialized information when combined with the noun. 2. The relational adjective has a semantic meaning which helps understand the conceptual relationship between the adjective and the noun being modified and disambiguate certain senses of the resulting lexical combination. 3. The relational adjective may impose some restrictions when choosing the nouns it modifies. Considering the above hypotheses, this study has identified and described a multi-word lexical unit pattern [Radj+n] referred to as a Specialized Lexical Combination (SLC) linguistically realized by a relational adjective, Radj, and a noun, n. The analysis of such a syntactic pattern is addressed from the framework of the Generative Lexicon (Pustojovsky, 1995). Such theory provides several levels of semantic description which help lexical decomposition performed generatively. These levels of semantic representation are connected through generative operations or generative devices which account for the compositional interpretation of any linguistic utterance in a given context. This study analyses these different levels and focuses on one of them, i.e. the qualia structure since it may encode the conceptual meaning of the syntactic pattern [Radj+n]. The semantic study of these two lexical items has ultimately confirmed the conceptual meaning of the relational adjective. A corpus made of online ICT articles from magazines written in English and Spanish – some being their translations - has been used for the word extraction. For this purpose some word processing software packages have been employed. Moreover online general language and specialized language dictionaries have been consulted. Search engines, namely WordNet Search 3.1, have been also exploited to find the semantic information of our lexical units. Online reference corpora in English and Spanish have been used for a contrastive analysis of our data. Finally our conclusions have confirmed our initial hypotheses, i.e. relational adjectives are specialized lexical units which together with the nouns are part of the knowledge representation of the ICT subject field. Proposals for new research have been made together with some other suggestions for the design of computer applications to visually show the conceptual meaning of certain lexical units.

Desarrollo de una plataforma de gestión de un laboratorio automatizado

El presente TFG está enmarcado en el contexto de la biología sintética (más concretamente en la automatización de protocolos) y representa una parte de los avances en este sector. Se trata de una plataforma de gestión de laboratorios autónomos. El resultado tecnológico servirá para ayudar al operador a coordinar las máquinas disponibles en un laboratorio a la hora de ejecutar un experimento basado en un protocolo de biología sintética. En la actualidad los experimentos biológicos tienen una tasa de éxito muy baja en laboratorios convencionales debido a la cantidad de factores externos que intervienen durante el protocolo. Además estos experimentos son caros y requieren de un operador pendiente de la ejecución en cada fase del protocolo. La automatización de laboratorios puede suponer un aumento de la tasa de éxito, además de una reducción de costes y de riesgos para los trabajadores en el entorno del laboratorio. En la presente propuesta se pretende que se dividan las distintas entidades de un laboratorio en unidades funcionales que serán los elementos a ser coordinados por la herramienta resultado del TFG. Para aportar flexibilidad a la herramienta se utilizará una arquitectura orientada a servicios (SOA). Cada unidad funcional desplegará un servicio web proporcionando su funcionalidad al resto del laboratorio. SOA es esencial para la comunicación entre máquinas ya que permite la abstracción del tipo de máquina que se trate y como esté implementada su funcionalidad. La principal dificultad del TFG consiste en lidiar con las dificultades de integración y coordinación de las distintas unidades funcionales para poder gestionar adecuadamente el ciclo de vida de un experimento. Para ello se ha realizado un análisis de herramientas disponibles de software libre. Finalmente se ha escogido la plataforma Apache Camel como marco sobre el que crear la herramienta específica planteada en el TFG. Apache Camel juega un papel importantísimo en este proyecto, ya que establece las capas de conexión a los distintos servicios y encamina los mensajes oportunos a cada servicio basándose en el contenido del fichero de entrada. Para la preparación del prototipo se han desarrollado una serie de servicios web que permitirán realizar pruebas y demostraciones de concepto de la herramienta en sí. Además se ha desarrollado una versión preliminar de la aplicación web que utilizará el operador del laboratorio para gestionar las peticiones, decidiendo que protocolo se ejecuta a continuación y siguiendo el flujo de tareas del experimento.---ABSTRACT---The current TFG is bound by synthetic biology context (more specifically in the protocol automation) and represents an element of progression in this sector. It consists of a management platform for automated laboratories. The technological result will help the operator to coordinate the available machines in a lab, this way an experiment based on a synthetic biological protocol, could be executed. Nowadays, the biological experiments have a low success rate in conventional laboratories, due to the amount of external factors that intrude during the protocol. On top of it, these experiments are usually expensive and require of an operator monitoring at every phase of the protocol. The laboratories’ automation might mean an increase in the success rate, and also a reduction of costs and risks for the lab workers. The current approach is hoped to divide the different entities in a laboratory in functional units. Those will be the elements to be coordinated by the tool that results from this TFG. In order to provide flexibility to the system, a service-oriented architecture will be used (SOA). Every functional unit will deploy a web service, publishing its functionality to the rest of the lab. SOA is essential to facilitate the communication between machines, due to the fact that it provides an abstraction on the type of the machine and how its functionality is implemented. The main difficulty of this TFG consists on grappling with the integration and coordination problems, being able to manage successfully the lifecycle of an experiment. For that, a benchmark has been made on the available open source tools. Finally Apache Camel has been chosen as a framework over which the tool defined in the TFG will be created. Apache Camel plays a fundamental role in this project, given that it establishes the connection layers to the different services and routes the suitable messages to each service, based on the received file’s content. For the prototype development a number of services that will allow it to perform demonstrations and concept tests have been deployed. Furthermore a preliminary version of the webapp has been developed. It will allow the laboratory operator managing petitions, to decide what protocol goes next as it executes the flow of the experiment’s tasks.

Molecular characterization of the phenylacetic acid catabolic pathway in Pseudomonas putida U: The phenylacetyl-CoA catabolon

Fourteen different genes included in a DNA fragment of 18 kb are involved in the aerobic degradation of phenylacetic acid by Pseudomonas putida U. This catabolic pathway appears to be organized in three contiguous operons that contain the following functional units: (i) a transport system, (ii) a phenylacetic acid activating enzyme, (iii) a ring-hydroxylation complex, (iv) a ring-opening protein, (v) a β-oxidation-like system, and (vi) two regulatory genes. This pathway constitutes the common part (core) of a complex functional unit (catabolon) integrated by several routes that catalyze the transformation of structurally related molecules into a common intermediate (phenylacetyl-CoA).

Structures of two molluscan hemocyanin genes: Significance for gene evolution

We present here the description of genes coding for molluscan hemocyanins. Two distantly related mollusks, Haliotis tuberculata and Octopus dofleini, were studied. The typical architecture of a molluscan hemocyanin subunit, which is a string of seven or eight globular functional units (FUs, designated a to h, about 50 kDa each), is reflected by the gene organization: a series of eight structurally related coding regions in Haliotis, corresponding to FU-a to FU-h, with seven highly variable linker introns of 174 to 3,198 bp length (all in phase 1). In Octopus seven coding regions (FU-a to FU-g) are found, separated by phase 1 introns varying in length from 100 bp to 910 bp. Both genes exhibit typical signal (export) sequences, and in both cases these are interrupted by an additional intron. Each gene also contains an intron between signal peptide and FU-a and in the 3′ untranslated region. Of special relevance for evolutionary considerations are introns interrupting those regions that encode a discrete functional unit. We found that five of the eight FUs in Haliotis each are encoded by a single exon, whereas FU-f, FU-g, and FU-a are encoded by two, three and four exons, respectively. Similarly, in Octopus four of the FUs each correspond to an uninterrupted exon, whereas FU-b, FU-e, and FU-f each contain a single intron. Although the positioning of the introns between FUs is highly conserved in the two mollusks, the introns within FUs show no relationship either in location nor phase. It is proposed that the introns between FUs were generated as the eight-unit polypeptide evolved from a monomeric precursor, and that the internal introns have been added later. A hypothesis for evolution of the ring-like quaternary structure of molluscan hemocyanins is presented.

Structure of the replication terminus-terminator protein complex as probed by affinity cleavage.

The replication terminator protein (RTP) of Bacillus subtilis is a homodimer that binds to each replication terminus and impedes replication fork movement in only one orientation with respect to the replication origin. The three-dimensional structure of the RTP-DNA complex needs to be determined to understand how structurally symmetrical dimers of RTP generate functional asymmetry. The functional unit of each replication terminus of Bacillus subtilis consists of four turns of DNA complexed with two interacting dimers of RTP. Although the crystal structure of the RTP apoprotein dimer has been determined at 2.6-A resolution, the functional unit of the terminus is probably too large and too flexible to lend itself to cocrystallization. We have therefore used an alternative strategy to delineate the three dimensional structure of the RTP-DNA complex by converting the protein into a site-directed chemical nuclease. From the pattern of base-specific cleavage of the terminus DNA by the chemical nuclease, we have mapped the amino acid to base contacts. Using these contacts as distance constraints, with the crystal structure of RTP, we have constructed a model of the DNA-protein complex. The biological implications of the model have been discussed.

Suicídio : uma análise epidemiológica : estudo retrospetivo da casuística da Delegação do Sul do Instituto Nacional de Medicina Legal e Ciências Forenses, I.P. (2007-2012)

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014

Neuropathology of Basal Ganglia and its role in the Parkinsonian syndromes with special reference to the Globus pallidus

The globus pallidus, together with the striatum (caudate nucleus and putamen), substantia nigra, nucleus accumbens, and subthalamic nucleus constitute the basal ganglia, a group of nuclei which act as a single functional unit. The basal ganglia have extensive connections to the cerebral cortex and thalamus and exert control over a variety of functions including voluntary motor control, procedural learning, and motivation. The action of the globus pallidus is primarily inhibitory and balances the excitatory influence of other areas of the brain such as the cerebral cortex and cerebellum. Neuropathological changes affecting the basal ganglia play a significant role in the clinical signs and symptoms observed in the ‘parkinsonian syndromes’ viz., Parkinson’s disease (PD), progressive supranuclear palsy (PSP), dementia with Lewy bodies (DLB), multiple system atrophy (MSA), and corticobasal degeneration (CBD). There is increasing evidence that different regions of the basal ganglia are differentially affected in these disorders. Hence, in all parkinsonian disorders and especially PD, there is significant pathology affecting the substantia nigra and its dopamine projection to the striatum. However, in PSP and MSA, the globus pallidus is also frequently affected while in DLB and CBD, whereas the caudate nucleus and/or putamen are affected, the globus pallidus is often spared. This chapter reviews the functional pathways of the basal ganglia, with special reference to the globus pallidus, and the role that differential pathology in these regions may play in the movement disorders characteristic of the parkinsonian syndromes.

The ageing ocular surface:challenges for biomaterials design and function

Changing demographics and in particular an increasingly ageing population, in combination with improved longevity, will have a major impact on changing the face of human diseases and likewise the demand for appropriate biomaterials. The ocular surface is a multifaceted system that combines to create a unique mucosal surface, which includes the cornea, conjunctiva, sclera and lids of the eye. Physical parameters such as the eyelids and eyelashes, combined with the numerous secretory glands that produce the complex tear film, act together to protect and maintain the cornea. Unfortunately an ageing tear film and lacrimal functional unit can lead to impairment of this magnificently orchestrated structure. No single mechanism or modification is responsible but, whatever the cause, the consequence is a reduction in tear stability. An uncompromised tear film is fundamental to a healthy ocular surface. In the face of progressively changing demographics and consequent requirements for medical intervention and medical device developments, it is important to understand what effects the ageing process has on these anterior ocular structures.

Gravidade do olho seco em pacientes internados em unidade de terapia intensiva: análise de conceito e construção de definições

Dry eye syndrome is a multifactorial disease of the tear film, resulting from the instability of the lacrimal functional unit that produces volume change, up or tear distribution. In patients in intensive care the cause is enhanced due to various risk factors, such as mechanical ventilation, sedation, lagophthalmos, low temperatures, among others. The study's purpose is to build an assessment tool of Dry Eye Severity in patients in intensive care units based on the systematization of nursing care and their classification systems. The aim of this study is to build an assessment tool of Dry Eye Severity in hospitalized patients in Care Unit Intensiva.Trata is a methodological study conducted in three stages, namely: context analysis, concept analysis, construction of operational definitions and magnitudes of nursing outcome. For the first step we used the methodological framework for Hinds, Chaves and Cypress (1992). For the second step we used the model of Walker and Avant and an integrative review Whitemore seconds, Knalf (2005). This step enabled the identification of the concept of attributes, background and consequent ground and the construction of the settings for the result of nursing severity of dry eye. For the construction of settings and operational magnitudes, it was used Psicometria proposed by Pasquali (1999). As a result of context analysis, visualized from the reflection that the matter should be discussed and that nursing needs to pay attention to the problem of eye injury, so minimizing strategies are created this event with a high prevalence. With the integrative review were located from the crosses 19 853 titles, selected 215, and from the abstracts 96 articles were read in full. From reading 10 were excluded culminating in the sample of 86 articles that were used to analyze the concept and construction of settings. Selected articles were found in greater numbers in the Scopus database (55.82%), performed in the United States (39.53%), and published mainly in the last five years (48.82). Regarding the concept of analysis were identified as antecedents: age, lagophthalmos, environmental factors, medication use, systemic diseases, mechanical ventilation and ophthalmic surgery. As attributes: TBUT <10s, Schimer I test <5 mm in Schimer II test <10mm, reduced osmolarity. As consequential: the ocular surface damage, ocular discomfort, visual instability. The settings were built and added indicators such as: decreased blink mechanism and eyestrain.

Investigating the encoding of visual stimuli by forming neural circuits in the cat primary visual cortex

Contexte La connectomique, ou la cartographie des connexions neuronales, est un champ de recherche des neurosciences évoluant rapidement, promettant des avancées majeures en ce qui concerne la compréhension du fonctionnement cérébral. La formation de circuits neuronaux en réponse à des stimuli environnementaux est une propriété émergente du cerveau. Cependant, la connaissance que nous avons de la nature précise de ces réseaux est encore limitée. Au niveau du cortex visuel, qui est l’aire cérébrale la plus étudiée, la manière dont les informations se transmettent de neurone en neurone est une question qui reste encore inexplorée. Cela nous invite à étudier l’émergence des microcircuits en réponse aux stimuli visuels. Autrement dit, comment l’interaction entre un stimulus et une assemblée cellulaire est-elle mise en place et modulée? Méthodes En réponse à la présentation de grilles sinusoïdales en mouvement, des ensembles neuronaux ont été enregistrés dans la couche II/III (aire 17) du cortex visuel primaire de chats anesthésiés, à l’aide de multi-électrodes en tungstène. Des corrélations croisées ont été effectuées entre l’activité de chacun des neurones enregistrés simultanément pour mettre en évidence les liens fonctionnels de quasi-synchronie (fenêtre de ± 5 ms sur les corrélogrammes croisés corrigés). Ces liens fonctionnels dévoilés indiquent des connexions synaptiques putatives entre les neurones. Par la suite, les histogrammes peri-stimulus (PSTH) des neurones ont été comparés afin de mettre en évidence la collaboration synergique temporelle dans les réseaux fonctionnels révélés. Enfin, des spectrogrammes dépendants du taux de décharges entre neurones ou stimulus-dépendants ont été calculés pour observer les oscillations gamma dans les microcircuits émergents. Un indice de corrélation (Rsc) a également été calculé pour les neurones connectés et non connectés. Résultats Les neurones liés fonctionnellement ont une activité accrue durant une période de 50 ms contrairement aux neurones fonctionnellement non connectés. Cela suggère que les connexions entre neurones mènent à une synergie de leur inter-excitabilité. En outre, l’analyse du spectrogramme dépendant du taux de décharge entre neurones révèle que les neurones connectés ont une plus forte activité gamma que les neurones non connectés durant une fenêtre d’opportunité de 50ms. L’activité gamma de basse-fréquence (20-40 Hz) a été associée aux neurones à décharge régulière (RS) et l’activité de haute fréquence (60-80 Hz) aux neurones à décharge rapide (FS). Aussi, les neurones fonctionnellement connectés ont systématiquement un Rsc plus élevé que les neurones non connectés. Finalement, l’analyse des corrélogrammes croisés révèle que dans une assemblée neuronale, le réseau fonctionnel change selon l’orientation de la grille. Nous démontrons ainsi que l’intensité des relations fonctionnelles dépend de l’orientation de la grille sinusoïdale. Cette relation nous a amené à proposer l’hypothèse suivante : outre la sélectivité des neurones aux caractères spécifiques du stimulus, il y a aussi une sélectivité du connectome. En bref, les réseaux fonctionnels «signature » sont activés dans une assemblée qui est strictement associée à l’orientation présentée et plus généralement aux propriétés des stimuli. Conclusion Cette étude souligne le fait que l’assemblée cellulaire, plutôt que le neurone, est l'unité fonctionnelle fondamentale du cerveau. Cela dilue l'importance du travail isolé de chaque neurone, c’est à dire le paradigme classique du taux de décharge qui a été traditionnellement utilisé pour étudier l'encodage des stimuli. Cette étude contribue aussi à faire avancer le débat sur les oscillations gamma, en ce qu'elles surviennent systématiquement entre neurones connectés dans les assemblées, en conséquence d’un ajout de cohérence. Bien que la taille des assemblées enregistrées soit relativement faible, cette étude suggère néanmoins une intrigante spécificité fonctionnelle entre neurones interagissant dans une assemblée en réponse à une stimulation visuelle. Cette étude peut être considérée comme une prémisse à la modélisation informatique à grande échelle de connectomes fonctionnels.

Investigating the encoding of visual stimuli by forming neural circuits in the cat primary visual cortex

Contexte La connectomique, ou la cartographie des connexions neuronales, est un champ de recherche des neurosciences évoluant rapidement, promettant des avancées majeures en ce qui concerne la compréhension du fonctionnement cérébral. La formation de circuits neuronaux en réponse à des stimuli environnementaux est une propriété émergente du cerveau. Cependant, la connaissance que nous avons de la nature précise de ces réseaux est encore limitée. Au niveau du cortex visuel, qui est l’aire cérébrale la plus étudiée, la manière dont les informations se transmettent de neurone en neurone est une question qui reste encore inexplorée. Cela nous invite à étudier l’émergence des microcircuits en réponse aux stimuli visuels. Autrement dit, comment l’interaction entre un stimulus et une assemblée cellulaire est-elle mise en place et modulée? Méthodes En réponse à la présentation de grilles sinusoïdales en mouvement, des ensembles neuronaux ont été enregistrés dans la couche II/III (aire 17) du cortex visuel primaire de chats anesthésiés, à l’aide de multi-électrodes en tungstène. Des corrélations croisées ont été effectuées entre l’activité de chacun des neurones enregistrés simultanément pour mettre en évidence les liens fonctionnels de quasi-synchronie (fenêtre de ± 5 ms sur les corrélogrammes croisés corrigés). Ces liens fonctionnels dévoilés indiquent des connexions synaptiques putatives entre les neurones. Par la suite, les histogrammes peri-stimulus (PSTH) des neurones ont été comparés afin de mettre en évidence la collaboration synergique temporelle dans les réseaux fonctionnels révélés. Enfin, des spectrogrammes dépendants du taux de décharges entre neurones ou stimulus-dépendants ont été calculés pour observer les oscillations gamma dans les microcircuits émergents. Un indice de corrélation (Rsc) a également été calculé pour les neurones connectés et non connectés. Résultats Les neurones liés fonctionnellement ont une activité accrue durant une période de 50 ms contrairement aux neurones fonctionnellement non connectés. Cela suggère que les connexions entre neurones mènent à une synergie de leur inter-excitabilité. En outre, l’analyse du spectrogramme dépendant du taux de décharge entre neurones révèle que les neurones connectés ont une plus forte activité gamma que les neurones non connectés durant une fenêtre d’opportunité de 50ms. L’activité gamma de basse-fréquence (20-40 Hz) a été associée aux neurones à décharge régulière (RS) et l’activité de haute fréquence (60-80 Hz) aux neurones à décharge rapide (FS). Aussi, les neurones fonctionnellement connectés ont systématiquement un Rsc plus élevé que les neurones non connectés. Finalement, l’analyse des corrélogrammes croisés révèle que dans une assemblée neuronale, le réseau fonctionnel change selon l’orientation de la grille. Nous démontrons ainsi que l’intensité des relations fonctionnelles dépend de l’orientation de la grille sinusoïdale. Cette relation nous a amené à proposer l’hypothèse suivante : outre la sélectivité des neurones aux caractères spécifiques du stimulus, il y a aussi une sélectivité du connectome. En bref, les réseaux fonctionnels «signature » sont activés dans une assemblée qui est strictement associée à l’orientation présentée et plus généralement aux propriétés des stimuli. Conclusion Cette étude souligne le fait que l’assemblée cellulaire, plutôt que le neurone, est l'unité fonctionnelle fondamentale du cerveau. Cela dilue l'importance du travail isolé de chaque neurone, c’est à dire le paradigme classique du taux de décharge qui a été traditionnellement utilisé pour étudier l'encodage des stimuli. Cette étude contribue aussi à faire avancer le débat sur les oscillations gamma, en ce qu'elles surviennent systématiquement entre neurones connectés dans les assemblées, en conséquence d’un ajout de cohérence. Bien que la taille des assemblées enregistrées soit relativement faible, cette étude suggère néanmoins une intrigante spécificité fonctionnelle entre neurones interagissant dans une assemblée en réponse à une stimulation visuelle. Cette étude peut être considérée comme une prémisse à la modélisation informatique à grande échelle de connectomes fonctionnels.

Energy requirements for the continuous biohydrogen production from Spirogyra biomass in a sequential batch reactor

The current energy market requires urgent revision for the introduction of renewable, less-polluting and inexpensive energy sources. Biohydrogen (bioH2) is considered to be one of the most appropriate options for this model shift, being easily produced through the anaerobic fermentation of carbohydrate-containing biomass. Ideally, the feedstock should be low-cost, widely available and convertible into a product of interest. Microalgae are considered to possess the referred properties, being also highly valued for their capability to assimilate CO2 [1]. The microalga Spirogyra sp. is able to accumulate high concentrations of intracellular starch, a preferential carbon source for some bioH2 producing bacteria such as Clostridium butyricum [2]. In the present work, Spirogyra biomass was submitted to acid hydrolysis to degrade polymeric components and increase the biomass fermentability. Initial tests of bioH2 production in 120 mL reactors with C. butyricum yielded a maximum volumetric productivity of 141 mL H2/L.h and a H2 production yield of 3.78 mol H2/mol consumed sugars. Subsequently, a sequential batch reactor (SBR) was used for the continuous H2 production from Spirogyra hydrolysate. After 3 consecutive batches, the fermentation achieved a maximum volumetric productivity of 324 mL H2/L.h, higher than most results obtained in similar production systems [3] and a potential H2 production yield of 10.4 L H2/L hydrolysate per day. The H2 yield achieved in the SBR was 2.59 mol H2/mol, a value that is comparable to those attained with several thermophilic microorganisms [3], [4]. In the present work, a detailed energy consumption of the microalgae value-chain is presented and compared with previous results from the literature. The specific energy requirements were determined and the functional unit considered was gH2 and MJH2. It was possible to identify the process stages responsible for the highest energy consumption during bioH2 production from Spirogyra biomass for further optimisation.

Resource use and greenhouse gas emissions from grain-finishing beef cattle in seven Australian feedlots: a life cycle assessment

Grain finishing of cattle has become increasingly common in Australia over the past 30 years. However, interest in the associated environmental impacts and resource use is increasing and requires detailed analysis. In this study we conducted a life cycle assessment (LCA) to investigate impacts of the grain-finishing stage for cattle in seven feedlots in eastern Australia, with a particular focus on the feedlot stage, including the impacts from producing the ration, feedlot operations, transport, and livestock emissions while cattle are in the feedlot (gate-to-gate). The functional unit was 1 kg of liveweight gain (LWG) for the feedlot stage and results are included for the full supply chain (cradle-to-gate), reported per kilogram of liveweight (LW) at the point of slaughter. Three classes of cattle produced for different markets were studied: short-fed domestic market (55–80 days on feed), mid-fed export (108–164 days on feed) and long-fed export (>300 days on feed). In the feedlot stage, mean fresh water consumption was found to vary from 171.9 to 672.6 L/kg LWG and mean stress-weighted water use ranged from 100.9 to 193.2 water stress index eq. L/kg LWG. Irrigation contributed 57–91% of total fresh water consumption with differences mainly related to the availability of irrigation water near the feedlot and the use of irrigated feed inputs in rations. Mean fossil energy demand ranged from 16.5 to 34.2 MJ lower heating values/kg LWG and arable land occupation from 18.7 to 40.5 m2/kg LWG in the feedlot stage. Mean greenhouse gas (GHG) emissions in the feedlot stage ranged from 4.6 to 9.5 kg CO2-e/kg LWG (excluding land use and direct land-use change emissions). Emissions were dominated by enteric methane and contributions from the production, transport and milling of feed inputs. Linear regression analysis showed that the feed conversion ratio was able to explain >86% of the variation in GHG intensity and energy demand. The feedlot stage contributed between 26% and 44% of total slaughter weight for the classes of cattle fed, whereas the contribution of this phase to resource use varied from 4% to 96% showing impacts from the finishing phase varied considerably, compared with the breeding and backgrounding. GHG emissions and total land occupation per kilogram of LWG during the grain finishing phase were lower than emissions from breeding and backgrounding, resulting in lower life-time emissions for grain-finished cattle compared with grass finishing.

Contributo para a caracterização das raças serpentinas e charnequeira

Esta dissertação retoma um estudo com 49 cabras e 45 cabritos da raça Serpentina, 24 cabras e 28 cabritos da raça Charnequeira, exploradas em regime extensivo. Pretende ser um contributo para a caracterização destas raças. Com este objectivo calculou-se a fertilidade que foi de 92% nas duas raças e a prolificidade foi de 160% (62,5% partos duplos) na Serpentina e de 141% (57,1% partos duplos) na Charnequeira. A mortalidade dos cabritos, do nascimento até ao desmame, foi de 15,7%, na Serpentina e de 6,6%, na Chamequeira. Os pesos das cobrições ao parto, na Serpentina aumentaram 17% e 13,6% na Chamequeira, apresentando variações significativas na raça, mas não entre raças. Os cabritos de partos simples obtiveram maiores ganhos médios diários do nascimento ao desmame. Não houve variações significativas nos pesos entre machos e fêmeas. Analisaram-se 24 carcaças de cabritos, machos inteiros. O Rendimento Corrigido foi de 52% para as duas e a relação Músculo/Osso de 2,13 (Charnequeira) e 2,12 (Serpentina). O Índice Compacidade para a Charnequeira foi de 11,78 e 11,29 para a Serpentina, evidenciando esta melhor conformação. Definiram-se os seguintes cortes nas carcaças: I-Pá; II-Perna; III- Costela+Sela; IV-Aba; V-Pescoço. Analisou-se o músculo quanto à gordura, proteína, cálcio e fósforo. ABSTRACT: This dissertation retrieves a study involving 49 goats and 45 kids of Serpentina breed, 24 goats and 28 kids of Chamequeira breed exploited in an extensive management. It is intended as a contribution to the characterization of these breeds. To this end, various reproductive parameters were calculated. The fertility stood at 92% in both breeds and the prolificacy in the breed Serpentina was 160% (62.5% in twin births) and in the breed of Chamequeira was 141% (57.1% in twin births). We noted a mortality rate in kids from birth to weaning higher in the Serpentina breed (15.7%), while the Chamequeira breed recorded 6.6%. The evolution of the weights during the mating period to childbirth, increasing 17% in the Serpentina breed and 13.6% in Chamequeira breed. We noted significant variations in each breed, but not between each other. The kids resulting of simple birth had higher average daily weight gain from birth to weaning. We did not note significant variations of weight between males and females. We analysed the carcasses of 24 male kids. The corrected yield was 52% for both breeds and the muscle-bone ratio was 2,13 (Chamequeira) and 2,12 (Serpentina). The compactness index for the Chamequeira breed was 11.78 and 11.29 for the Serpentina breed, showing Serpentina breed had better conformation. We proposed the following cuts in the carcass: I-Shovel, II Leg, III- Rib +Sela, IV-Aba and V-Neck. We also analysed the muscle according to their fat, protein, calcium and phosphorus, aiming to show the dietary interest of this muscle.

Environmental assessment of industrial chemical and primary sector processes from a life cycle perspective

During the PhD program in chemistry at the University of Bologna, the environmental sustainability of some industrial processes was studied through the application of the LCA methodology. The efforts were focused on the study of processes under development, in order to assess their environmental impacts to guide their transfer on an industrial scale. Processes that could meet the principles of Green Chemistry have been selected and their environmental benefits have been evaluated through a holistic approach. The use of renewable sources was assessed through the study of terephthalic acid production from biomass (which showed that only the use of waste can provide an environmental benefit) and a new process for biogas upgrading (whose potential is to act as a carbon capture technology). Furthermore, the basis for the development of a new methodology for the prediction of the environmental impact of ionic liquids has been laid. It has already shown good qualities in identifying impact trends, but further research on it is needed to obtain a more reliable and usable model. In the context of sustainable development that will not only be sector-specific, the environmental performance of some processes linked to the primary production sector has also been evaluated. The impacts of some organic farming practices in the wine production were analysed, the use of the Cereal Unit parameter was proposed as a functional unit for the comparison of different crop rotations, and the carbon footprint of school canteen meals was calculated. The results of the analyses confirm that sustainability in the industrial production sector should be assessed from a life cycle perspective, in order to consider all the flows involved during the different phases. In particular, it is necessary that environmental assessments adopt a cradle-to-gate approach, to avoid shifting the environmental burden from one phase to another.