Impact of Epstein-Barr virus load, virus genotype, and frequency of the 30bp deletion in the viral BNLF-1 gene in patients harboring the human immunodeficiency virus

Patients infected with the human immunodeficiency virus (HIV) are at higher risk of developing Epstein-Barr Virus (EBV)-associated lymphomas. The usefulness of monitoring EBV in peripheral blood mononuclear cells (PBMCs) of patients infected with HIV has not been established. The aim of this study was to evaluate the EBV viral load in PBMCs, the frequency of viral genotypes, and the presence of the 30-bp deletion in the BNLF-1 gene. DNA samples from 156 patients attending the HIV/AIDS Day Clinic at Botucatu School of Medicine, Sao Paulo State University were evaluated. The EBV viral load was detectable by real time PCR in 123/156 (78.8%) cases and was higher in patients not receiving antiretroviral treatment or under therapeutic failure than in patients under successful highly active antiretroviral therapy (HAART) (P=0.0076). Overall, the profile of patients with high EBV viral load included elevated HIV viremia (P=0.0005), longer time of HIV diagnosis (P=0.0026), and increased levels of T CD8 + lymphocytes (P=0.0159). The successful amplification of the EBNA-2 gene by nested-PCR was achieved in 95 of 123 (77.2%) cases, of which 75.8% were EBV-1, 9.5% EBV-2, and 14.7% were co-infected with both EBV-1 and -2. The analysis of the BNLF-1 gene was possible in 99 of 123 (80.5%) cases, of which 50.5% had the 30-bp deletion. EBV-1 was more common than EBV-2, which may reflect the fact that the cohort was predominantly Caucasian and heterosexual. J. Med. Virol. 85:2110-2118, 2013. © 2013 Wiley Periodicals, Inc.

Load models support sustainable: planning for Brazil s reservoir aquaculture

Cage aquaculture in hydroelectric reservoirs has great potential for expansion in Brazil, but there are concerns of negative environmental impacts. The environmental sustainability of cage culture depends on hamornization between farming practices and the hydrological peculiarities of the site. Mass balance modeling can estimate the amounts of nutrients that can be loaded without triggering eutrophication and resulting maximum allowable production volume. Careful climate zoning can also assist proper siting.

A load-balancing spare capacity reallocation approach in service-rich SONET metro mesh networks

The next-generation SONET metro network is evolving into a service-rich infrastructure. At the edge of such a network, multi-service provisioning platforms (MSPPs) provide efficient data mapping enabled by Generic Framing Procedure (GFP) and Virtual Concatenation (VC). The core of the network tends to be a meshed architecture equipped with Multi-Service Switches (MSSs). In the context of these emerging technologies, we propose a load-balancing spare capacity reallocation approach to improve network utilization in the next-generation SONET metro networks. Using our approach, carriers can postpone network upgrades, resulting in increased revenue with reduced capital expenditures (CAPEX). For the first time, we consider the spare capacity reallocation problem from a capacity upgrade and network planning perspective. Our approach can operate in the context of shared-path protection (with backup multiplexing) because it reallocates spare capacity without disrupting working services. Unlike previous spare capacity reallocation approaches which aim at minimizing total spare capacity, our load-balancing approach minimizes the network load vector (NLV), which is a novel metric that reflects the network load distribution. Because NLV takes into consideration both uniform and non-uniform link capacity distribution, our approach can benefit both uniform and non-uniform networks. We develop a greedy loadbalancing spare capacity reallocation (GLB-SCR) heuristic algorithm to implement this approach. Our experimental results show that GLB-SCR outperforms a previously proposed algorithm (SSR) in terms of established connection capacity and total network capacity in both uniform and non-uniform networks.

Impact of viral load and the duration of primary infection on HIV transmission

Abstract Objectives: HIV 'treatment as prevention' (TasP) describes early treatment of HIV-infected patients intended to reduce viral load and transmission. Crucial assumptions for estimating TasP's effectiveness are the underlying estimates of transmission risk. We aimed to determine transmission risk during primary infection, and of the relation of HIV transmission risk to viral load. Design: A systematic review and meta-analysis. Methods: We searched PubMed and Embase databases for studies that established a relationship between viral load and transmission risk, or primary infection and transmission risk, in serodiscordant couples. We analysed assumptions about the relationship between viral load and transmission risk, and between duration of primary infection and transmission risk. Results: We found 36 eligible articles, based on six different study populations. Studies consistently found that larger viral loads lead to higher HIV transmission rates, but assumptions about the shape of this increase varied from exponential increase to saturation. The assumed duration of primary infection ranged from 1.5 to 12 months; for each additional month, the log10 transmission rate ratio between primary and asymptomatic infection decreased by 0.40. Conclusion: Assumptions and estimates of the relationship between viral load and transmission risk, and the relationship between primary infection and transmission risk, vary substantially and predictions of TasP's effectiveness should take this uncertainty into account.

25.0 mph 26 degree crabbed moving barrier impact tests into a 26 degree load cell barrier face. Test #1: NHTSA deformable barrier face. Test #2: EEVC deformable barrier face. Test report.

Mode of access: Internet.

Cost and economic impact of tire reserve load requirements. Final report.

National Highway Traffic Safety Administration, Washington, D.C.

Extending the service life of existing bridges by increasing their load carrying capacity /

Mode of access: Internet.

Operator and organizational maintenance manual for truck, lift, fork, diesel engine, pneumatic tired wheels, rough terrain, 6,000 lb. capacity, 24 inch load center (Anthony model MLT-6, Army model MHE 200), NSN 3930-00-903-0900 ....

"July 1980."

Organizational maintenance repair parts and special tools lists : truck, lift, fork, diesel engine, pneumatic tired wheels, rough terrain, 6,000 lb capacity, 24 inch load center, Army model MHE 200, NSN 3930-00-903-0900, Anthoney model MLT-6, NSN 3930-01-054-3831, Anthoney model MLT-6 with ROPS ...

Includes index.

Direct support, general support, and depot maintenance manual : truck, lift, fork, diesel engine, pneumatic tired wheels, rough terrain, 6,000 lb capacity, 24 inch load center, (Anthony model MLT-6, Army model MHE-200) ...

"March 1974."

Direct support and general support maintenance repair parts and special tools lists (including depot maintenance repair parts and special tools lists) : truck, lift, fork, diesel engine, pneumatic-tired wheels, rough terrain, 10,000 lb. capacity, 24 in. load center, Army model MHE 199, NSN 3930-00-903-0899 ....

Includes index.

Direct support and general support maintenance repair parts and special tools lists (including depot maintenance repair parts and special tools lists) : truck, lift, fork, diesel engine, pneumatic tired wheels, rough terrain, 6,000 lb. capacity, 24 in. load center ...

"Army model MHE 200, NSN 3930-00-903-0900, Anthoney model MLT-6, NSN 3930-01-054-3831, Anthoney model MLT-6 with ROPS and Army model MHE 202, NSN 3930-00-937-0220, Chrysler model MLT-6CH, NSN 3930-01-053-4823, Chrysler model MLT-6CH with ROPS and Army model MHE 222, NSN 3930-00-419-5744, Athey model ARTFT-6, NSN 3930-01-054-3830, Athey model ART FT-6 with ROPS."

Organizational maintenance repair parts and special tools lists : truck, lift, fork, diesel engine, pneumatic-tired wheels, rough terrain, 10,000 lb. capacity, 24 in. load center, Army model MHE 199, NSN 3930-00-903-0899, Pettibone-Mulliken model RTL10 ...

Includes index.

Operator and organizational maintenance manual : truck, lift, fork, diesel engine, pneumatic tired wheels, rough terrain, 10,000 lb. capacity, 24 inch load center (Pettibone-Mulliken model RTL10, Army model MHE 199), FSN 3930-903-0899 ....

"June 1971."