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Information is given about the results of the latest meeting of the Herring Assessment Working Group for the Area South of 62° N and the Baltic Fisheries Assessment Working Group, which were conducted in March and April at ICES headquarters in Copenhagen. The actual stock development and the catch options estimated for different assessment units of herring and sprat are presented. The spawning stock size of North Sea herring reached in 1998 for the first time since 1991 a level above the Minimum Biological Acceptable Level (MBAL) of 800 000 t. Keeping the fishery at the present level the spawning stock size should reach in 1999 a level of about 1.2 mio. t. For the Western Baltic Spring spawning herring stock no actual stock estimate was presented. Due to uncertainties in splitting the catches in North Sea autumn and Baltic spring spawners no analytical assessment was accepted.

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Trials for the determination of the magnitude of bycatch reduction by the sievenets used in the commercial brown shrimp fishery were carried out from September to December 1997. Trawls with 9 m beam length were the subjected to the investigation. They were used on different fishing grounds in the estuary of the Elbe near Cuxhaven. Sievenets with 50, 60 and 80 mm mesh opening were tested in 29 hauls and 31.6 h total duration. A trawl of equal construction without sievenets fished synchronously was used for comparison. The proportional catch composition in the codend was determined by weighing the catch components (fish, discard shrimps and commercial size shrimps) as separated by means of a riddle. The composition of the sorted out part of the catch could be calculated by comparison of the corresponding catch components both in standard trawl and sievenettrawl. According to this the total catch of a beam trawl with sievenet is diminished by 9 to 34 % depending on the mesh opening of the sievenet. 32 to 58 % of the sorted out part of the catch consists of fish. Use of a sievenet, however, also leads to a loss of 6 to 15 % of commercial size shrimps. Per hour of towing this means a loss of 8.7 kg commercial size shrimps with a sievenet of 60 mm mesh opening and of 1.8 kg for a mesh size of 80 mm.

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Raw or smoked eel was analysed by isoelectric focusing of sarcoplasmic proteins. For raw fish specific protein patterns were obtained for A. anguilla/A. rostrata, A. japonica and A. australis, but in case of smoked fish differentiation was only possible between Atlantic and Pacific species. Differentiation of raw or smoked eel was possible by PCR-SSCP, but patterns of ssDNA showed some intra-specific variability depending on the type of amplicon.

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An EU funded research project was started in 1998 by institutes from Ireland, Belgium, the Netherlands and Germany to reduce the adverse environmental impact of demersal trawls. In the frame of this project the Institute for Fishery Technique of the Federal Research Centre for Fisheries, Hamburg, is developing a jet beamtrawl replacing the heavy tickler chains of a traditional flatfish beam trawl by water jet nozzles placed at the lower side of the beam with the jets directed towards the sea bottom. First trials on the dutch research vessel “Tridens” were performed in March 1998. Catch and bycatch of a jet beamtrawl and a traditional beamtrawl were compared. The efficiency of the jet beamtrawl was not satisfactory and will have to be improved.

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Deutscher Caviar, made from roe of lumpfish or capelin, gives species specific patterns in protein electrophoresis. The same techniques can be used to differentiate caviar from salmon and trout. The differentiation of sturgeon caviar (beluga, osietra, sevruga) is possible by isoelectric focusing, but not by SDS-PAGE. PCR-based methods of DNA-analysis for identification of the origin of sturgeon caviar are under development.

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The evaluation of heated mussels by protein analysis makes clear that the methods used allow a differentiation between mussel tissues which had experienced different degrees of heating. It could be verifiyed by both, the analysis of enzyme activities and differential scanning calorimetry that the investigated trade products were only blanched and not completely well done cooked.

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Bei den hydroakustischen Untersuchungen der in der westlichen Ostsee pelagisch lebenden Fischarten, Hering und Sprott, traten in der Vergangenheit große Unterschiede zwischen den Tag- und Nachtmeßwerten auf. Während der 327. Reise des FFK "Solea" wurden in einem Teil der Reise Untersuchungen durchgeführt, die diesen Sachverhalt näher beleuchten. Es wurde gefunden daß, die Unterschiede in den Integratorwerten, speziell in diesem Gebiet, im wesentlichen durch die vertikale Migration der Fische im Tagesverlauf hervorgerufen werden. Während des Tages halten sie sich in der Nähe des Bodens auf und sind damit technisch bedingt zu einem großen Teil einer hydroakustischen Erfassung nicht zugänglich, wogegen die Nachtmessungcn reproduzierbare Werte ergeben. Aus diesem Grund sind die hydroakustischen Aufnahmen in der westlichen Ostsee wegen der größeren zu erwartenden Meßgenauigkeit nachts durchzuführen.

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Seitdem die wirtschaftlich wichtigen Rundfischbestände in der Nord- und Ostsee immer stärker zurückgehen, ist wiederholt eine Intensivierung der Heringsfischerei vorgeschlagen worden. Zur Begründung dieses Vorschlages wird angeführt, daß sich die Heringsbestände in einem relativ guten Zustand befinden und daß die Deutschland zugeteilten Heringsquoten bislang weitgehend unausgeschöpft geblieben sind. So betrug die deutsche Heringsquote beispielsweise 1990 in der Nordsee 50 000 t, gefangen wurden aber lediglich ca. 5OOO t. In neuester Zeit hat allerdings der Einsatz großer Schwarmfischfänger eine bessere Quotennutzung bewirkt.

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In the last years farmed Pangasius (Tra-Pangasius, Pangasius hypophthalmus) from Vietnam has reached a considerable market share, whereas aquaculture of Asian Redtail Catfish (Hemibagrus wyckioides) is in its infancy. Recently it has been detected by food control authorities in Hamburg, that Pangasius fillets have been mislabelled and sold as fillets produced from Asian Redtail catfish. The necessity to improve the analytical methods for differentiation of Pangasius and Redtail Catfish prompted us to evaluate the suitability of isoelectric focusing (IEF) and DNA-analysis for identification of the two species. IEF of water soluble proteins was found to be a fast, reliable and economical method for differentiation of raw fillets of Pangasius and Redtail Catfish, as long as reference material is available. PCR-based DNA analysis was performed as follows: (i) amplification of a 464 bp segment of the cytochrome b gene; (ii) sequencing of the PCR product; (iii) comparison of the sequence with entries in GenBank using BLAST. The sequences of both species differed considerably, allowing the unequivocal differentiation between P. hypophthalmus and H. wyckioides. Kurzfassung Pangasius (Schlankwels, Tra-Pangasius, Pangasius hypophthalmus) hat sich innerhalb weniger Jahre zu einem bedeutenden Zuchtfisch entwickelt, während die Aquakultur des Asiatischen Rotflossenwelses (Hemibagrus wyckioides) in Vietnam noch in einem relativ kleinen Maßstab stattfindet. Kürzlich wurde von der Lebensmittelüberwachung in Hamburg nachgewiesen, dass im Handel erhältliche Filets mit der Deklaration „Rotflossenwels“ aus Pangasius hergestellt worden waren. Vor diesem Hintergrund wurden zwei Methoden auf ihre Eignung zur Differenzierung von Pangasius und Rotflossenwels geprüft. Es zeigte sich, dass sowohl die isoelektrische Fokussierung (IEF) wasserlöslicher Proteine als auch die PCR-basierte DNA-Analyse zur Unterscheidung beider Arten gut geeignet ist. Die IEF stellt eine schnelle und kostengünstige Untersuchungsmethode dar, die allerdings Referenzmaterial benötigt. Mit Hilfe der PCR (Polymerase-Kettenreaktion) wurde ein Abschnitt des Cytochrom b-Gens vervielfältigt und sequenziert. Die Sequenzen von P. hypophthalmus und H. wyckioides wiesen beträchtliche Unterschiede auf. Es wird diskutiert, wie sich durch Vergleich dieser Sequenzen mit Einträgen in Gendatenbanken unbekannte Proben beider Arten sicher zuordnen lassen.