WISP genes are members of the connective tissue growth factor family that are up-regulated in Wnt-1-transformed cells and aberrantly expressed in human colon tumors

Wnt family members are critical to many developmental processes, and components of the Wnt signaling pathway have been linked to tumorigenesis in familial and sporadic colon carcinomas. Here we report the identification of two genes, WISP-1 and WISP-2, that are up-regulated in the mouse mammary epithelial cell line C57MG transformed by Wnt-1, but not by Wnt-4. Together with a third related gene, WISP-3, these proteins define a subfamily of the connective tissue growth factor family. Two distinct systems demonstrated WISP induction to be associated with the expression of Wnt-1. These included (i) C57MG cells infected with a Wnt-1 retroviral vector or expressing Wnt-1 under the control of a tetracyline repressible promoter, and (ii) Wnt-1 transgenic mice. The WISP-1 gene was localized to human chromosome 8q24.1–8q24.3. WISP-1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors and its RNA overexpressed (2- to >30-fold) in 84% of the tumors examined compared with patient-matched normal mucosa. WISP-3 mapped to chromosome 6q22–6q23 and also was overexpressed (4- to >40-fold) in 63% of the colon tumors analyzed. In contrast, WISP-2 mapped to human chromosome 20q12–20q13 and its DNA was amplified, but RNA expression was reduced (2- to >30-fold) in 79% of the tumors. These results suggest that the WISP genes may be downstream of Wnt-1 signaling and that aberrant levels of WISP expression in colon cancer may play a role in colon tumorigenesis.

Report of the treasurer of the Committee of Relief for the Sufferers by the Fall of the Pemberton Mill, in Lawrence, Mass, on the 10th of January, 1860

Signed, p. 19: Charles S. Storrow.

Pacific Mills, Lawrence, Mass., Dover, N.H., Columbia, S.C... are the largest manufacturers in the world of cotton goods

Lawrence & Co.

[Admittatur of William Lawrence (Harvard AB 1743)], 1739

Notebook with a handwritten copy of the 1735 College laws in English prepared by Harvard undergraduate William Lawrence and signed by President Edward Holyoke, Fellow Nathan Prince, and Tutor Daniel Rogers on September 9, 1739.

The Speck collection of Montagnais material culture from the Lower St. Lawrence Drainage, Quebec / James W. VanStone, Curator, North American Archaeology and Ethnology, Field Museum of Natural History.

n.s. no.5(1982)

Relationships of pocket gophers of the genus Geomys from the Central and Northern Great Plains / by Lawrence R. Heaney and Robert M. Timm.

no.74 (1983)

Occasional papers of the Natural History Museum, the University of Kansas, Lawrence, Kansas.

no.173 (1995)

Occasional papers of the Natural History Museum, the University of Kansas, Lawrence, Kansas.

no.171 (1994)

Occasional papers of the Natural History Museum, the University of Kansas, Lawrence, Kansas.

no.174 (1995)