Metabolismo oxidativo de neutrófilos em ovelhas naturalmente infectadas por nematódeos gastrintestinais e correlação entre nível sérico de cortisol e carga parasitária

The relationships between neutrophils oxidative metabolism, cortisol serum levels and worm burden, estimated by fecal egg count (EPG), were studied in sheep naturally infected by gastrointestinal parasites at the end of pregnancy, during lactation, and after weaning. Twenty-two Suffolk sheep three to four year old, of same parity and season of parturition were used. Serum cortisol was determined by radioimmunoassay and the neutrophils oxidative metabolism by the nitroblue tetrazolium (NBT) reduction test. The highest EPG values were observed on the fifth week of lactation while the highest rates of cortisol and reduction of NBT occurred on the fourth week after weaning. A positive correlation (r = 0.52; P<0.01) was observed between the neutrophils capacity to reduce the NBT and the serum cortisol concentration in the pre-parturition period. Otherwise, the neutrophils oxidative metabolism decreased near to the parturition. A negative correlation (r = -0.39; P<0.01) between EPG and NBT reduction test was observed after weaning, which was coincident with the increase in the neutrophils capacity to reduce NBT, indicating that animals showing better immune response after weaning have neutrophils with higher oxidative metabolism and lower worm burden.

Comparação entre limiar anaeróbio determinado por variáveis ventilatórias e pela resposta do lactato sanguíneo em ciclistas

Many investigations have shown that the coincidence between the ventilatory thresholds and those thresholds using the lactate response does not happen all of the time, suggesting that there is no relationship between the cause-effect between these phenomena. Thus, the present study had as main purpose to compare and correlate the Oxygen consumption (V̇O 2), the power (W), and the heart rate (HR) values attained using protocols to determine the Ventilatory Threshold (VT) and the Individual Anaerobic Threshold (IAT). The sampling was constituted by eight State and National level cyclists (age: 27.88 ± 8.77 years; body mass: 65.19 ± 4.40 kg; height: 169.31 ± 5,77 cm). The IAT was determined starting from a three minutes 50 W warm up with progressive increases of 50 W.3min -1 up to achieving the voluntary exhaustion, when the blood was collected in the last 20 seconds of each phase, and during the recovering period. In order to determine the VT, it was used the same protocol used to determine the IAT, but without performing the blood collection. The VT was identified through the changes in the pulmonary ventilation, as well as of the ventilatory equivalent of the O 2 and CO 2. The t-Student test showed no significant statistical difference in any of the attained variables. The associations found were high and significant. The V̇O 2 (ml.kg -1.min. -1), P (W), and HR (bpm) corresponding to the VT and IAT, as well as the associations between variables were respectively: 48.00 ± 3.82 vs. 48.08 ± 3.71 (r = 0.90); 256.25 ± 32.04 vs. 246.88 ± 33.91 (r = 0.84); 173.75 ± 9.18 vs. 171.25 ± 12.02 (r = 0.97). According to the results attained, it can be concluded that the IAT and the VT produce similar V̇O 2, W, and HR values, favoring the adoption of the VT because it is a non-invasive method to determine the anaerobic threshold in cyclists.

Atividades séricas da aspartato aminotransferase, creatina quinase e lactato desidrogenase de eqüinos submetidos ao teste padrão de exercício progressive em esteira

The aim of this study was to determine the serum activities of enzymes aspartate aminotransferase, creatine kinase and lactate dehydrogenase in Arabian horses submitted to exercise on high-speed equine treadmill. Eleven mature Arabian horse were training and submitted to Standard Incremental Exercise Test on high-speed equine treadmill. Venous blood samples were taken before exercise, immediately and 30 min, 60min, 3h, 6h, 24h, 3 days and 5 days after exercise. The serum activity aspartate aminotransferase, creatine kinase and lactate dehydrogenase were determined. The serum activies of AST, CK and LDH increase immediately and returned to baseline value 30 minutes after exercise. The AST enzyme activity increased at 12 hours and 24 hours, CK at 3 hours and 6 hours, and LDH at 24 hours after Standard Incremental Exercise Test.

Perfil lipídico sérico de ratos tratados com surfactante

Atherosclerosis is a multifactorial, progressive and slow disease, and hyperlipidaemia is one of the potential factors in the development of atherosclerotic cardiac diseases. The experimental dyslipidaemia carrying out advantages are the production of atheromatous lesions in a short period of time, an adequate dietetic control and environmental factors, the possibility of studies concerning reversibility of atherosclerotic lesions, and pre-clinic experiments with hypolipidaemic substances. This study aims at evaluating tyloxapol analyzing serum lipid levels. Twenty-eight healthy Wistar adults' albino male rats, weighing an average of 200 g were utilized. They were distributed into four experimental groups with seven animals each, as follows: Group I - (control); Group II - treated with tyloxapol at a dose of 500mg/kg of body weight, through intraperitoneal via each 48 hours, for two weeks; Group III - treated with tyloxapol at a dose of 500mg/ kg of body weight, through intraperitoneal via each 48 hours, for three weeks; Group IV - treated with tyloxapol at a dose of 500mg/kg of body weight, through intraperitoneal via each 48 hours, for four weeks. As lipid profile evaluation is concerned, the values of triacylglycerols and HDL have indicated that group III has significantly differed from group I and the values of total cholesterol and LDL have indicated that group I has significantly differed from group II, III and IV. It was concluded that for the studied period the surfactant tyloxapol was effective to inducing hyperlipidaemia.

Concentrações séricas de aspartato aminotransferase e creatinoquinase e concentrações plasmáticas de lactato em equinos da raça Mangalarga Marchador após exercício físico

The aim of this study was evaluate the influence of physical exercise (marcha gait) on serum values of CK and AST and plasmatic values of lactate in Mangalarga Marchador horses trained in Espirito Santo, Brazil. Serum and plasma samples were obtained from 15 horses in four different moments: rest (T0), 5 minutes (T1), 30 minutes (T2) and 2 hours (T3) after the exercise. Lactate analysis revealed values of 1.02 ± 0.41 mmol/L, 2.73 ± 2.43 mmol/L, 1.89 ± 1.24 mmol/L and 1.31 ± 0.60 mmol/L, respectively at T0, T1, T2 and T3. When evaluating AST, the results recorded in T0, T1, T2 and T3 were, respectively, 189.3 ± 56.0 UI/L, 223.9 ± 53.5 UI/L, 186.8 ± 25.8 UI/L and 193.9 ± 44.7 UI/L. Finally, the CK at moments T0, T1, T2 and T3 were, respectively, 113.4 ± 56.3 UI/l, 144.1 ± 70.9 UI/L, 143.0 ± 81.0 UI/L and 173.1 ± 128.0 UI/L. The results showed that marcha gait leaded to significantly increased in plasma lactate and did not alter serum AST and CK, suggesting that the equines used were conditioned to the physical exercised imposed.

Influência da seleção dos estágios incrementais sobre a intensidade de lactato mínimo: Estudo piloto

The purposes of this study were to assess the influence of stage selection from the incremental phase and the use of peak lactate after hyperlactatemia induction on the determination of the lactate minimum intensity (iLACmin). Twelve moderately active university students (23±5 years, 78.3±14.1 kg, 175.3±5.1 cm) performed a maximal incremental test to determine the respiratory compensation point (RCP) (initial intensity at 70 W and increments of 17.5 W every 2 minutes) and a lactate minimum test (induction with the Wingate test, the incremental test started at 30 W below RCP with increments of 10 W every 3 minutes) on a cycle ergometer. The iLACmin was determined using second order polynomial adjustment applying five exercise stage selection: 1) using all stages (iLACmin P); 2) using all stages below and two stages above iLACminP(iLACminA); 3) using two stages below and all stages above iLACminP(iLACminB); 4) using the largest and same possible number of stages below and above the iLACminP(iLACminI); 5) using all stages and peak lactate after hyperlactatemia induction (iLACminD). No differences were found between the iLACminP(138.2±30.2 W), iLACminA(139.1±29.1 W), iLACminB(135.3±14.2 W), iLACminI(138.6±20.5 W) and iLACmiD(136.7±28.5 W) protocols, and a high level of agreement between these intensities and iLACminPwas observed. Oxygen uptake, heart rate, rating of perceived exertion and lactate corresponding to these intensities was not different and was strongly correlated. However, the iLACminBpresented the lowest success rate (66.7%). In conclusion, stage selection did not influence the determination of iLACmin but modified the success rate. © Creative Commom.

Influência de diferentes tipos de recuperação sobre a modulação autonômica cardíaca, concentração de lactato e proteína C-reativa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Validação de um teste específico para determinação do máximo déficit acumulado de oxigênio (MAOD) e da máxima fase estável de lactato em nado atado

Pós-graduação em Fisioterapia - FCT

Correlações entre os valores de lactato mínimo e potência anaeróbia com a potência crítica e a capacidade de trabalho anaeróbio obtidas por método invasivo e não invasivos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da luminosidade e do horário do exercício de natação até a exaustão na intensidade de lactato mínimo sobre respostas bioquímicas e hematológicas de ratos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Validade do teste do lactato mínimo para a determinação do limiar anaeróbio em ratos durante exercício de natação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Respostas metabólicas e da técnica de nado durante o exercício realizado na velocidade correspondente à máxima fase estável de lactato sanguíneo determinada de forma contínua e intermitente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Predição da intensidade de corrida em máxima fase estável de lactato a partir da velocidade de crítica em atletas fundistas de alto rendimento: relações com performances

Pós-graduação em Ciências da Motricidade - IBRC

Interleucina 6 e proteinograma sérico de ovinos submetidos à endotoxemia experimental

Pós-graduação em Ciência Animal - FMVA