Production of reactive oxygen (H2O2) and nitrogen (NO) intermediates and TNF-α in mice genetically selected for high (H) and low (L) antibody response and experimentally infected with Leptospira serovar pomona

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Toxoplasma gondii and Leptospira spp. infection in free-ranging armadillos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Response activity of alveolar macrophages in pulmonary dysfunction caused by Leptospira infection

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anticorpos para Leptospira spp., Toxoplasma gondii e Neospora caninum em cães errantes albergados em canil privado

The serological profile of 300 mongrel dogs of various ages and gender were investigated. Animals were captured in the streets and afterwards directed to a private kennel in Avare city (SP) to search for leptospirosis, toxoplasmosis, and neosporosis. Blood samples were obtained from jugular or cephalic vein for the obtention of sera. The microscopic agglutination test (MAT) was used to leptospirosis. MAT detect the prevalence of 9.3%. The most frequent reactant serovars were Bratislava (35.7%), Cynopteri (17.9%), Autumnalis (14.3%), and Copenhageni (10.7%), besides 7.1% to others serovars: Icterohaemorrhagiae, Canicola, and Hardjo. The modified agglutination test used for the diagnosis of toxoplasmosis showed 26% of positive animals, with titers varying from 16 to 256, with 16 in 3.3%, 64 in 13.7%, and 256 in 9% of the samples. To canine neosporosis, it was used the indirect fluorescent antibody test, and two animals (0.7%) demonstrated antibodies with titers 25 and 100. The results show the participation of the animals in the epidemiological chain of the researched diseases.

Detecção de Leptospira pomona em sêmen bovino por eletroforese capilar fluorescente

This study was performed in order to evaluate the detection limit of PCR with fluorescent capillary electrophoresis for Leptospira pomona diagnosis in bovine semen. Negative bovine semen samples were artificially contaminated with Leptospira pomona (10 0 to 10 7 bacteria/ ml) and DNA was extracted by phenol/chloroform protocol. DNA fragments visualization was done by three electrophoresis methods: under UV light in 2 % agarose gel, silver staining 8% polyacrylamide gel and fluorescent capillary electrophoresis. The detection limit of capillary electrophoresis for Leptospira pomona was 10 2bacteria/ml. Under UV light, in 2 % agarose gel, the detection limit was of 10 4 bacteria/ ml while for silver stained 8 % polyacrylamide gel it was 10 2 bacteria/ ml. PCR with fluorescent capillary electrophoresis is an efficient and rapid diagnostic test for DNA detection of Leptospira in bovine semen and this can be an important tool for herd and semen sanitary control in artificial insemination centers.

Avaliação de fatores de risco de cães sororreagentes à leptospira spp. e sua distribuição espacial, em área territorial urbana

We aimed to evaluate the risk factor of serum reactive dogs to antileptospire agglutinin and their spatial distribution in an urban area. We collected 1,000 blood samples from dogs at 20 immunization centers, homogeneously distributed in the urban area (32 km²) of Botucatu, Sao Paulo, Brazil. Diagnosis was made by microscopic serum agglutination using 24 serovars of Leptospira spp. Statistical analysis was performed by Goodman's test with 5% of significance. Spatial clusters were tested by spatial scan statistic using SaTScan. We found that 17.9% dogs reacted to Leptospira spp. (p<0.0001). Dogs with street access (22.14%) were more reactive (p<0.05) than those without (14.83%). The scan test indicated only one significant cluster influenced by age. Incorporating each one of the co-variables gender, breed, and street access to age, we found that street access was the most important. Street access and age were the most important risk factors in the large number of reactive animals in the cluster, with the castellonis serovar being the most reactive in urban dogs. The identification of a cluster with more reactive dogs than expected allows local preventive measures to be taken.

Anticorpos anti-Leptospira spp. em animais selvagens do zoológico municipal de Ribeirão Preto, Estado de São Paulo, Brasil

Leptospirosis may affect all domestic and wild animals as well as human beings. Some serological studies have shown the involvement of wild species in the epidemiology of the disease. Once captive wild animals are not much studied, especially in Brazil, the present study aimed to detect anti- Leptospira spp. antibodies in animals from Ribeirão Preto city zoo, in São Paulo state, Brazil. Blood samples were collected from captive birds, fish, reptiles and mammals, sinanthropics and free-living animals and also from employees between March and October, 2006. Four hundred and three blood samples were obtained, 388 animals' samples (110 reptiles, 143 birds, 110 mammals and 25 fish) and 15 humans'. The sera were analysed by Microscopic Agglutination Test using 22 serovars from pathological leptospiras and two from non-pathological serovars. Among the animal samples, 339 were from captive animals, and 49 from free-living ones, captured with traps inside the zoo. One hundred and three (103/388 = 26.5%) samples reacted to leptospirosis, ninety-two (92/339 = 27.1%) samples were from captive animals and eleven (11/49 = 22.4%) from free-living ones. All humans' samples were negative. Serological titles varied from 40 to 5.120, with predominance of titles between 40 and 80 and the most frequent serovars were Patoc, Andamana, Canicola, Icterohaemorrhagiae and Panama.

Variáveis epidemiológicas e alterações clínicas, hematológicas e urinárias em cães sororreagentes para Leptospira spp

In order to determine antibodies anti-Leptospira spp. in dogs from Botucatu-SP with a clinical suspicion and the risk factors for this zoonosis, as well possible hematological and urine alterations were collected blood samples from 248 dogs. The diagnostic test performed was the Microscopic Agglutination Test (MAT) with 29 serovars of leptospires. Titers were considered reagent ≥200 and detected in 17.7% (44/248) of the dogs. The most frequent serovars were Autumnalis (20.5%); Pyrogenes (18.2%); Gryppothyphosa (15.9%); Canicola (13.6%); Bratislava and Copenhageni (9.1%); Andamana (4.5%) and Djasiman (2.3%). Regarding the analysis of the epidemiological questionnaire administered to owners of the dogs, there was statistical significance for sex, age and presence of rats at home, showing that, by logistic regression analysis, only the sex variable was been significantly associated to the presence of antibodies anti-Leptospira. The levels of urea and creatinine were significantly increased in the group of animals reagents to the Microscopic Agglutination Test, showing the renal damage in these animals and decreased hemoglobin level.

Isolamento e reação em cadeia pela polimerase (PCR) para Leptospira spp. em amostras renais e hepáticas de ovinos sorologicamente positivos e negativos para leptospirose, abatidos em frigorífico, procedentes do estado de São Paulo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aglutininas contra Leptospira spp em cervos-do-pantanal (Blastocerus dichotomus) na área afetada pela construção da usina hidrelétrica Sérgio Motta

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Padronização de um protocolo para detecção molecular de Leptospira spp. e Brucella spp. em sêmen bovino comercial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Freqüência de anticorpos contra Leptospira spp. em felídeos neotropicais em cativeiro no Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Pesquisa de anticorpos anti-Leptospira spp, Toxoplasma gondii e Neospora caninum em cães recolhidos das ruas e albergados em canil privado de Avaré (SP)

Pós-graduação em Medicina Veterinária - FMVZ

Infecção por Leptospira spp. e Neospora caninum, em rebanho bovino leiteiro: avaliação dos aspectos produtivos e reprodutivos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)