Effects of a bifenthrin-treated net on the natural enemies Aphidius colemani (Haliday) and Adalia bipunctata L. in a cucumber crop in Central Spain. Semi-field experiments

Pest management practices that rely on pesticides are growing increasingly less effective and environmentally inappropriate in many cases and the search of alternatives is under focus nowadays. Exclusion of pests from the crop by means of pesticide-treated screens can be an eco-friendly method to protect crops, especially if pests are vectors of important diseases. The mesh size of nets is crucial to determine if insects can eventually cross the barrier or exclude them because there is a great variation in insect size depending on the species. Long-lasting insecticide-treated (LLITN) nets, factory pre-treated, have been used since years to fight against mosquitoes vector of malaria and are able to retain their biological efficacy under field for 3 years. In agriculture, treated nets with different insecticides have shown efficacy in controlling some insects and mites, so they seem to be a good tool in helping to solve some pest problems. However, treated nets must be carefully evaluated because can diminish air flow, increase temperature and humidity and decrease light transmission, which may affect plant growth, pests and natural enemies. As biological control is considered a key factor in IPM nowadays, the potential negative effects of treated nets on natural enemies need to be studied carefully. In this work, the effects of a bifentrhin-treated net (3 g/Kg) (supplied by the company Intelligent Insect Control, IIC) on natural enemies of aphids were tested on a cucumber crop in Central Spain in autumn 2011. The crop was sown in 8x6.5 m tunnels divided in 2 sealed compartments with control or treated nets, which were simple yellow netting with 25 mesh (10 x 10 threads/cm2; 1 x 1 mm hole size). Pieces of 2 m high of the treated-net were placed along the lateral sides of one of the two tunnel compartments in each of the 3 available tunnels (replicates); the rest was covered by a commercial untreated net of a similar mesh. The pest, Aphis gossypii Glover (Aphidae), the parasitoid Aphidius colemani (Haliday) (Braconidae) and the predator Adalia bipunctata L. (Coccinellidae) were artificially introduced in the crop. Weekly sampling was done determining the presence or absence of the pest and the natural enemies (NE) in the 42 plants/compartment as well as the number of insects in 11 marked plants. Environmental conditions (temperature, relative humidity, UV and PAR radiation) were recorded. Results show that when aphids were artificially released inside the tunnels, neither its number/plant nor their distribution was affected by the treated net. A lack of negative effect of the insecticide-treated net on natural enemies was also observed. Adalia bipunctata did not establish in the crop and only a short term control of aphids was observed one week after release. On the other hand, A. colemani did establish in the crop and a more long-term effect on the numbers of aphids/plant was detected irrespective of the type of net. KEY WORDS: bifenthrin-treated net, Adalia bipunctata, Aphidius colemani, Aphis gossypii, semi-field

Tyrosine replacement in P-selectin glycoprotein ligand-1 affects distinct kinetic and mechanical properties of bonds with P- and L-selectin

Selectins are adhesion molecules that initiate tethering and rolling of leukocytes on the vessel wall. Rolling requires rapid formation and breakage of selectin–ligand bonds that must have mechanical strength to resist premature dissociation by the forces applied in shear flow. P- and L-selectin bind to the N-terminal region of P-selectin glycoprotein ligand-1 (PSGL-1), a mucin on leukocytes. To define determinants on PSGL-1 that contribute to the kinetic and mechanical properties of bonds with selectins, we compared rolling of transfected preB cells expressing P- or L-selectin on transfected cell monolayers expressing wild-type PSGL-1 or PSGL-1 constructs with substitutions in targeted N-terminal residues. Rolling through P- or L-selectin required a Thr or Ser at a specific position on PSGL-1, the attachment site for an essential O-glycan, but required only one of three nearby Tyr residues, which are sites for Tyr-SO3 formation. The adhesive strengths and numbers of cells rolling through P- or L-selectin were similar on wild-type PSGL-1 and on each of the three PSGL-1 constructs containing only a single Tyr. However, the cells rolled more irregularly on the single-Tyr forms of PSGL-1. Analysis of the lifetimes of transient tethers on limiting densities of PSGL-1 revealed that L-selectin dissociated faster from single-Tyr than wild-type PSGL-1 at all shears examined. In sharp contrast, P-selectin dissociated faster from single-Tyr than wild-type PSGL-1 at higher shear but not at lower shear. Thus, tyrosine replacements in PSGL-1 affect distinct kinetic and mechanical properties of bonds with P- and L-selectin.

Les XV. livres de la Metamorphose d'Ovide, poete treselegant : contenants l'Olympe des hystoires poetiqves, traduitz de latin en françois.

Page numbers 383-384 repeated.

Séances et travaux de l'Académie des sciences morales et politiques, compte rendu.

The "Séances et travaux" of the academy for 1840 and 1841 were published in 1 vol. in 1893.

Nomination of Wendy L. Gramm [microform] : hearing before the Committee on Agriculture, Nutrition, and Forestry, United States Senate, One Hundredth Congress, second session on the nomination of Wendy L. Gramm, of Texas, to be Chairman of the Commodity Futures Trading Commission, February 3, 1988.

CIS Microfiche Accession Numbers: CIS 88 S161-26

Le Bulletin de l'art ancien et moderne.

Issues for 1900- called also 2e- année; 17e-20e année skipped in numbering while publication was suspended.

L'observateur.

Tome 2 has subtitle: Journal historique, littéraire et politique.

Revue générale de l'électricité.

At head of title, 1966-1995: RGE.

Generalized Ramsey numbers for stars /

"UIUCDCS-R-81-1050"--Cover.

Implementation of P.L. 96-480 Stevenson-Wydler Technology Innovation Act of 1980 [microform] : hearings (including an analysis of the hearings prepared for the subcommittee by the Congressional Research Service) before the Subcommittee on Science, Research, and Technology of the Committee on Science and Technology, U.S. House of Representatives, Ninety-seventh Congress, first session, July 14, 15, 16, 1981.

"No. 81."

Mémoires de la Société académique d'agriculture, des sciences, arts et belles-lettres du département de l'Aube.

Tome 14- also called 2.sér. t.1-14, 1847-63; 3.sér. t.1- 1864-

L'architecture françoise, ou, Recueil des plans, elevations, coupes et profils des églises, palais, hôtels & maisons particulieres de Paris, & des chasteaux & maisons de campagne ou de plaisance des environs, & de plusieurs autres endroits de France, bâtis nouvellement par les plus habils architectes, et levés & mesurés exactement sur les lieux.

L'architecture française : réimpression de l'édition originale de 1727", Paris, G. Vanoest, 1927-1929, shows 562 plates in v. 1-3. Guilmard lists 540 plates (more than one to a leaf in some instances).

Calculating tables, giving the products of every two numbers from one to one thousand and their application to the multiplication and division of all numbers above one thousand.

Added t.-p. in French and German. 9. ed 1904. p. III-X in French and German in parallel columns.

L'Afrique française; bulletin mensuel du Comité l'Afrique française et du Comité du Maroc.

Beginning with 1914, the numbers are occasionally combined.