EU Facilitated Dialogue: Another exercise in constructive ambiguity. CEPS Commentary, 28 August 2015

Under the auspices of the EU, Belgrade and Pristina have signed new agreements on how to further normalise their relations. Yet these agreements, including on the creation of an Association/Community of Serb majority municipalities in Kosovo, are the continuation of an exercise in constructive ambiguity, argues Dusan Reljic in this CEPS Commentary. He makes the point that the proper implementation of these much heralded agreements will be something of a challenge.

Shan zuo bei mu /

On double leaves, oriental style.

Ming san shi jia shi xuan.

Er ji has special title page.

Jing shi wen da : [10 juan /

Juan duan ti : Quan Xieshan xian sheng jing shi wen da.

Kongzi miao tang bei kao /

Juan duan guan: Kongzi miao tang bei Tang ben cun zi, 6 p. (1st group)

Jing yun lou ji : 12 juan /

Block print.

Shi jing xiao xue : 30 juan /

On double leaves, oriental style, in case.

Zhou li Han du kao : 6 juan /

On double leaves, oriental style, in case.

Shuo wen jie zi zhu : 15 juan /

On double leaves, oriental style, in case.

Hui lan ji yao : 2 juan ; An lan ji yao : 2 juan /

Block print.

Cao Yuechuan xian sheng yi shu /

Double leaves, oriental style, in case.

Shang shu gu wen zhuan yi : 32 juan /

On double leaves, oriental style, in case.

Yi du jin shi ji : si juan /

Reissued: 2 ban ; Tai bei : Xin wen feng chu ban gong si, Min'guo 71 [1982]

Polymorphisms in the 5 '-untranslated region of the human serotonin receptor 1B (HTR1B) gene affect gene expression

We present evidence of complex balancing regulation of HTR1B transcription by common polymorphisms in its promoter. Computational analysis of the HTR1B gene predicted that a 50 segment, spanning common DNA sequence variations, T-261G, A-161T, and -182INS/DEL-181, contained a putative functional promoter. Using a secreted alkaline phosphatase (SEAP) reporter gene system, we found that the haplotype -261G_-182INS-181_A-161 enhanced transcriptional activity 2.3-fold compared with the haplotype T-261_-182INS-181_A-161. Conversely, -161T reversed this, and the net effect when -261G and -161T were in the same haplotype (-261G_-182INS-181_-161T) was equivalent to the major haplotype (T-261_-182INS-181_A-161). Electrophoretic mobility shift experiments showed that -261G and -161T modify the binding of transcription factors (TFs): -261G generates a new AP2 binding site, while alleles A-161 and -161T exhibit different binding characteristics to AP1. T-261G and A-161T were found to be in linkage disequilibrium (LD) with G861C in a European ancestry population. Interestingly, G861C has been reported to be associated with several psychiatric disorders. Our results indicate that HTR1B is the target of substantial transcriptional genetic regulation by common haplotypes, which are in LD with the HTR1B single-nucleotide polymorphism (SNP) most commonly used in association studies.

Advances in understanding of enzymatic browning in harvested litchi fruit

Litchi (Litchi chinensis Sonn.) is a subtropical to tropical fruit of high commercial value in international trade. However, harvested litchi fruit rapidly lose their bright red skin colour. Peel browning of harvested litchi fruit has largely been attributed to rapid degradation of red anthocyanin pigments. This process is associated with enzymatic oxidation of phenolics by polyphenol oxidase (PPO) and/or peroxidase (POD). PRO and POD from litchi pericarp cannot directly oxidize anthocyanins. Moreover, PPO substrates in the pericarp are not well characterised. Consequently, the roles of PPO and POD in litchi browning require further investigation. Recently, an anthocyanase catalysing the hydrolysis of sugar moieties from anthocyanin to anthocyanidin has been identified in litchi peel for the first time. Thus, litchi enzymatic browning may involve an anthocyanase-anthocyanin-phenolic-PPO reaction. Current research focus is on characterising the properties of the anthocyanase involved in anthocyanin degradation. Associated emphasis is on maintenance of membrane functions in relation to loss of compartmentation between litchi peel oxidase enzymes and their substrates. (C) 2004 Elsevier Ltd. All rights reserved.