A genomic assisted breeding program for cassava to improve nutritional quality and industrial traits of storage root

A mandioca é cultivada como "mandioca mansa" para consumo in natura e "mandioca para indústria" como fonte de amidos e farinhas. Raças locais foram utilizadas para descoberta de "mutações espontâneas" e desenvolvimento de abordagem evolutiva e de melhoramento para estudos de função gênica. Recursos de Genômica e Proteômica foram obtidos. Análises de expressão gênica por blot de RNA e microarranjos foram desenvolvidos para identificação de expressão diferencial. "Mandioca açucarada" foi identificada, sendo relacionada com falta de expressão do gene da BEI e de uma mutação "nonsence" na sequência do gene GBSSI causando a formação do amido serose. "Mandioca avermelhada" apresentou falta de expressão do gene CasLYB, e a "amarela" uma regulação de repressão do gene CasHYb. Análise Proteômica do complexo carotenóide-proteína, juntamente com a análise de expressão de gene da CAP4, revelaram uma dupla fita de cDNA associada ao elevado acúmulo de carotenóide. Sequenciamento do gene da GBSSI identificou 22 haplótipos e grande diversidade de nucleotídios. Populações segregantes de cruzamentos de fenótipos bioquímicos diferenciados com cultivares elites dos Cerrados foram obtidas.

Mineração industrial na Amazônia e suas implicações para o desenvolvimento regional

Embora a mercantilização de recursos minerais na Amazônia oriental brasileira tenha envolvido a comercialização, por mais de cinco décadas, de mercadorias bastante diversas, extraídas e transformadas industrialmente por empresas que foram instaladas em momentos históricos distintos, suas repercussões no desenvolvimento de certas áreas da região guardam importantes singularidades e têm lógicas e características, em grande parte, comuns. O trabalho sintetiza os macrocenários nos quais se instalaram empresas mínero-metal úrgicas e analisa as razões das limitações que as atividades desenvolvidas por essas empresas têm para impulsionar dinâmicas de desenvolvimento amplas e socialmente enraizadas.

Trabalho e trabalhadores da pesca industrial no Pará face à metamorfose do capital

O mundo do trabalho passa por grandes transformações, as quais englobam novas tecnologias e formas de organização dos fatores produtivos no processo de produção de mercadorias. Novos modelos de organização do trabalho têm surgido e se mesclado com as práticas fordistas, o que tem influenciado diretamente sobre a utilização da mão-de-obra no processo produtivo. Neste contexto, este estudo procurou analisar de que maneira as práticas do processo de reestruturação produtiva estão acontecendo em um setor de atividade: o da pesca industrial, levando em consideração as particularidades do desenvolvimento dessa atividade extrativa, da forma de estruturação das empresas que compõem este setor para se manter no mercado e/ou ganhar novos mercados e, a maneira de inserção dos trabalhadores nas atividades de captura e processamento da produção pescada. A análise foi realizada entre as empresas que constituem o parque industrial pesqueiro do Distrito de Icoraci, que concentra a maior parcela da pesca industrial no Estado do Pará, estudando de que forma as estratégias adotadas pelas indústrias processadoras e microempresas de captura, diante das mudanças no mercado consumidor e da diminuição dos principais recursos explorados, têm repercutido sobre os trabalhadores ocupados nas atividades de captura e processamento.

Dinâmica espaço-temporal da pesca industrial de camarão-rosa Farfantepenaeus subtilis na plataforma continental do Amazonas

O conhecimento da distribuição espacial dos recursos pesqueiros e um fator essencial no ordenamento pesqueiro. O camarão-rosa Farfantepenaeus subtilis (Perez-Farfante, 1967) e uma espécie de importância econômica, capturado pela pesca industrial na Plataforma Continental do Amazonas. Este estudo teve como objetivo avaliar padrões espaços-temporais da abundancia relativa desse recurso a partir de uma serie de capturas realizadas por barcos da frota industrial, especializadas com o uso de ferramentas de sistema de informações geográficas. A abundancia relativa de camarão-rosa (CPUE) foi relacionada a batimetria, as características do substrato, a vazão do rio Amazonas e as variáveis oceanográficas obtidas por sensoriamento: temperatura da superfície do mar e concentração de clorofila-a. Entre as categorias de tipo de substrato, observou-se maior intensidade de arrastos na região de lama mosqueada. Nessa região, características como tipo de substrato (lama), relevo submarino, taxa de sedimentação (<1 cm.ano-1), e salinidade (>30) constituem o habitat ideal para o camarão-rosa. Maiores valores de CPUE estiveram associados a menores temperaturas e a maiores valores de concentração de clorofila-a, características que ocorrem no período de maior vazão do rio Amazonas, no primeiro semestre. Foi observada a ocorrência de três períodos com diferentes níveis de produção: fevereiro a abril (maior vazão), com maior abundancia relativa de camarão-rosa; maio a julho; e agosto a setembro (menor vazão), com menor abundancia. Os resultados mostraram que a abundancia relativa de F. subtilis não se distribui de modo uniforme no espaço nem na variação sazonal.

Geotecnologias aplicadas ao ordenamento territorial pesqueiro industrial do estuário amazônico

O presente trabalho pretende refletir geograficamente sobre a atividade pesqueira, apresentando algumas categorias de análise territorial que podem ser trabalhadas na pesca, levando em consideração os modos de vida dos pescadores; a abrangência territorial sob sua influencia da pesca e os mecanismos de representação espacial em ambiente computadorizado que estão disponíveis na atualidade, capazes de alcançar os padrões espaciais dessa abrangência. Esta análise partiu da consideração de que a atividade pesqueira é de extrema importância para o abastecimento dos centros urbanos, sendo a principal fonte de subsistência e de renda de populações de pescadores artesanais na região amazônica. Novos procedimentos metodológicos e processos tecnológicos têm engendrado à pesca uma significância cada vez maior, tanto pela aparente exaustão dos recursos, quanto pelo reconhecimento protéico e funcional, que os produtos pesqueiros têm ganhado nos últimos anos, como forma de alimentação adequada para a manutenção da qualidade de vida do consumidor. Dessa forma, o principal objetivo desse trabalho está em verificar e discutir sobre a viabilidade do uso de geotecnologias no atual modelo de ordenamento pesqueiro que se observa na região amazônica, buscando entender como esse ordenamento territorial na pesca pode integrar: geotecnologias; informações sobre equipamentos de pesca utilizados na captura do pescado; o conhecimento de pescadores e a legislação brasileira vigente. As pesquisas bibliográficas e de campo (na baía do Caeté e no rio Ituquara, estado do Pará), integradas aos trabalhos em laboratório, com utilização de técnicas de geoprocessamento sobre produtos do sensoriamento remoto permitiram mapear o momento dinâmico de algumas relações sócio-espaciais e estruturais por que passa a pesca, por isto, é importante o enfoque em mecanismos de auxilio ao ordenamento dos recursos pesqueiros. As tecnologias da chamada ciência da geoinformação, vem apresentando maior visibilidade nos estudos ambientais e por isto devem ser inseridas na atividade pesqueira como importante mecanismo de monitoramento, fiscalização e pesquisa em prol de manejos futuros.

Meio século de mineração industrial na Amazônia e suas implicações para o desenvolvimento regional

A IMPLANTAÇÃO na Amazônia oriental brasileira, nas últimas cinco décadas, de empresas voltadas à extração e à transformação industrial de minerais acalentou, recorrentemente, em diversos e amplos segmentos sociais, expectativas de rápida industrialização regional como derivação da utilização de "vantagens comparativas" decorrentes do atendimento de demandas globais de mercadorias minerais. Todavia, as atividades mínero-metalúrgicas tiveram enormes limitações em impulsionar dinâmicas de desenvolvimento amplas e socialmente enraizadas, o que resulta, em grande medida, das trajetórias e dinâmicas de inovação tecnológica a que são vinculadas, da ambiência institucional na qual estão inseridas e da pouca importância que é requerida das especificidades sociais, culturais e ecológicas locais para garantir a competitividade da valorização pouco qualificada de recursos minerais da região.

Ensino de gestão da capacidade e da empresa com o apoio do jogo Mercado Virtual no curso de Mestrado Integrado em Engenharia Industrial e Gestão da UMINHO/Azurém e no Programa de Pós-Graduação em Engenharia de Produção da FEB/Bauru/UNESP

O jogo de empresas Mercado Virtual foi desenvolvido para mediar o processo ensino-aprendizagem na área de tomada decisão e gestão de empresas e tem um banco de dados que armazena as decisões dos alunos. Os dados nele armazenados foram analisados em relação ao balanceamento de capacidade, objetivo de lucro e uso de recursos financeiros da empresa e foram encontradas incoerências entre os conteúdos pertinentes ao modelo e as decisões dos alunos. Elas foram classificadas como lacunas de aprendizado. Com o objetivo de analisar se as mesmas se repetem entre alunos do Programa de Mestrado Engenharia de Produção da UNESP de Bauru e Mestrado Integrado em Engenharia Industrial e Gestão da Uminho de Guimarães, Portugal, foram realizados dois experimentos, um em cada grupo. Para realizá-los utilizou-se o jogo Mercado Virtual e uma planilha de dimensionamento da empresa nos dois locais. A Sala de Estudos, os indicadores e o questionário de pesquisa da opinião foram utilizados somente em Portugal. Os resultados mostraram que o jogo é capaz de evidenciar as diferenças de domínio de conteúdo nos dois grupos e, também, que estas diferenças estão associadas aos projetos dos cursos. Sendo uma pesquisa exploratória, os experimentos foram realizados considerando-se somente os controles do jogo. Por isso, propõe-se a realização de pesquisas adicionais combinando controle sobre algumas das variáveis relacionadas do uso do jogo e atuação sobre aprendizagem dos alunos na forma de entrevistas e pesquisas não estruturadas. Conforme previsto, a pesquisa mostrou que os jogos podem ser utilizados com objetivos de aprendizagem mais amplos, considerando-se a avaliação indireta e cruzada sobre as decisões tomadas pelos alunos, como é o caso dos indicadores. Outra contribuição importante da pesquisa refere-se ao uso de jogos de empresas sob condições pouco controladas, ou seja, não houve...

Avaliação ambiental em empresas do distrito industrial Antônio Crepaldi, Presidente Prudente/SP

The sustainable development is the center of many discussions about the environment, economy and society. Environmental issues bring out problems with the fauna and flora, water resources, land use, population growth, residues, basic sanitation, and others. In this sense, the aim of the present work was to develop an environmental diagnostics and propose measures to mitigate the most significant environmental impacts generated by four enterprises in Antonio Crepaldi Industrial District, located at the city of Presidente Prudente/SP. In the study, it was used the methodology proposed by Moreira (2006), and it was done the following activities: monitoring and analyses of the production process, identification of potential environmental aspects and impacts; assessment of the impacts according to its nature, relevance and significance; proposition of measures to mitigate the critical impacts. As a final result, it was obtained an environmental diagnosis about the situation of each of the four companies studied, and it was possible to know the level of criticality of the impacts in each one, and proposing mitigation measures to control or minimizing them, bringing many benefits to the organization

Diagóstico ambiental de empreendimentos do distrito industrial Antônio Crepaldi – Presidente Prudente/SP

Recently, the environmental concern has grown due to the higher awareness about the availability and the importance of natural resources for survival on the Earth. So, laws and regulations were implemented aiming the environmental preservation. It has being developed new systems, technologies and techniques in order to minimize wastage of resources and minimize waste generation that may cause environmental impacts, in order to abide by the laws and regulations. These techniques and systems have been developed mostly for the industrial sector, because it’s one of the most responsible for the waste generation and impacts. One of the most employed systems is the environmental management, performed during the environmental diagnosis of the area/activity. So, this study aimed the environmental assessment and the establishment of proposals for the mitigation of the most significant environmental impacts generated in five industries of the Antonio Crepaldi industrial district – Presidente Prudente, in order to contribute to environmental issues and provide greater efficiency in the productive process of these industries... (Complete abstract click electronic access below)

Evaluation of the genotoxicity of waters impacted by domestic and industrial effluents of a highly industrialized region of So Paulo State, Brazil, by the comet assay in HTC cells

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de metodologia para implementação de eficiência energética em sistemas auxiliares de uma planta industrial siderúrgica semi-integrada

Pós-graduação em Engenharia Mecânica - FEG

Catalytic liquid- and gas-phase oxidations for the synthesis of intermediates and specialty chemicals: some examples of industrial relevance

Nowadays, it is clear that the target of creating a sustainable future for the next generations requires to re-think the industrial application of chemistry. It is also evident that more sustainable chemical processes may be economically convenient, in comparison with the conventional ones, because fewer by-products means lower costs for raw materials, for separation and for disposal treatments; but also it implies an increase of productivity and, as a consequence, smaller reactors can be used. In addition, an indirect gain could derive from the better public image of the company, marketing sustainable products or processes. In this context, oxidation reactions play a major role, being the tool for the production of huge quantities of chemical intermediates and specialties. Potentially, the impact of these productions on the environment could have been much worse than it is, if a continuous efforts hadn’t been spent to improve the technologies employed. Substantial technological innovations have driven the development of new catalytic systems, the improvement of reactions and process technologies, contributing to move the chemical industry in the direction of a more sustainable and ecological approach. The roadmap for the application of these concepts includes new synthetic strategies, alternative reactants, catalysts heterogenisation and innovative reactor configurations and process design. Actually, in order to implement all these ideas into real projects, the development of more efficient reactions is one primary target. Yield, selectivity and space-time yield are the right metrics for evaluating the reaction efficiency. In the case of catalytic selective oxidation, the control of selectivity has always been the principal issue, because the formation of total oxidation products (carbon oxides) is thermodynamically more favoured than the formation of the desired, partially oxidized compound. As a matter of fact, only in few oxidation reactions a total, or close to total, conversion is achieved, and usually the selectivity is limited by the formation of by-products or co-products, that often implies unfavourable process economics; moreover, sometimes the cost of the oxidant further penalizes the process. During my PhD work, I have investigated four reactions that are emblematic of the new approaches used in the chemical industry. In the Part A of my thesis, a new process aimed at a more sustainable production of menadione (vitamin K3) is described. The “greener” approach includes the use of hydrogen peroxide in place of chromate (from a stoichiometric oxidation to a catalytic oxidation), also avoiding the production of dangerous waste. Moreover, I have studied the possibility of using an heterogeneous catalytic system, able to efficiently activate hydrogen peroxide. Indeed, the overall process would be carried out in two different steps: the first is the methylation of 1-naphthol with methanol to yield 2-methyl-1-naphthol, the second one is the oxidation of the latter compound to menadione. The catalyst for this latter step, the reaction object of my investigation, consists of Nb2O5-SiO2 prepared with the sol-gel technique. The catalytic tests were first carried out under conditions that simulate the in-situ generation of hydrogen peroxide, that means using a low concentration of the oxidant. Then, experiments were carried out using higher hydrogen peroxide concentration. The study of the reaction mechanism was fundamental to get indications about the best operative conditions, and improve the selectivity to menadione. In the Part B, I explored the direct oxidation of benzene to phenol with hydrogen peroxide. The industrial process for phenol is the oxidation of cumene with oxygen, that also co-produces acetone. This can be considered a case of how economics could drive the sustainability issue; in fact, the new process allowing to obtain directly phenol, besides avoiding the co-production of acetone (a burden for phenol, because the market requirements for the two products are quite different), might be economically convenient with respect to the conventional process, if a high selectivity to phenol were obtained. Titanium silicalite-1 (TS-1) is the catalyst chosen for this reaction. Comparing the reactivity results obtained with some TS-1 samples having different chemical-physical properties, and analyzing in detail the effect of the more important reaction parameters, we could formulate some hypothesis concerning the reaction network and mechanism. Part C of my thesis deals with the hydroxylation of phenol to hydroquinone and catechol. This reaction is already industrially applied but, for economical reason, an improvement of the selectivity to the para di-hydroxilated compound and a decrease of the selectivity to the ortho isomer would be desirable. Also in this case, the catalyst used was the TS-1. The aim of my research was to find out a method to control the selectivity ratio between the two isomers, and finally to make the industrial process more flexible, in order to adapt the process performance in function of fluctuations of the market requirements. The reaction was carried out in both a batch stirred reactor and in a re-circulating fixed-bed reactor. In the first system, the effect of various reaction parameters on catalytic behaviour was investigated: type of solvent or co-solvent, and particle size. With the second reactor type, I investigated the possibility to use a continuous system, and the catalyst shaped in extrudates (instead of powder), in order to avoid the catalyst filtration step. Finally, part D deals with the study of a new process for the valorisation of glycerol, by means of transformation into valuable chemicals. This molecule is nowadays produced in big amount, being a co-product in biodiesel synthesis; therefore, it is considered a raw material from renewable resources (a bio-platform molecule). Initially, we tested the oxidation of glycerol in the liquid-phase, with hydrogen peroxide and TS-1. However, results achieved were not satisfactory. Then we investigated the gas-phase transformation of glycerol into acrylic acid, with the intermediate formation of acrolein; the latter can be obtained by dehydration of glycerol, and then can be oxidized into acrylic acid. Actually, the oxidation step from acrolein to acrylic acid is already optimized at an industrial level; therefore, we decided to investigate in depth the first step of the process. I studied the reactivity of heterogeneous acid catalysts based on sulphated zirconia. Tests were carried out both in aerobic and anaerobic conditions, in order to investigate the effect of oxygen on the catalyst deactivation rate (one main problem usually met in glycerol dehydration). Finally, I studied the reactivity of bifunctional systems, made of Keggin-type polyoxometalates, either alone or supported over sulphated zirconia, in this way combining the acid functionality (necessary for the dehydrative step) with the redox one (necessary for the oxidative step). In conclusion, during my PhD work I investigated reactions that apply the “green chemistry” rules and strategies; in particular, I studied new greener approaches for the synthesis of chemicals (Part A and Part B), the optimisation of reaction parameters to make the oxidation process more flexible (Part C), and the use of a bioplatform molecule for the synthesis of a chemical intermediate (Part D).

Structure-property relations in polymers from renewable resources

The present research project focuses its attention on the study of structure-property relations in polymers from renewable sources (bio-based polymers) such as polymers microbially produced, i.e. polyhydrohyalkanoates (PHAs) or chemically synthesized using monomers from renewable sources, i.e. polyammide 11 (PA11). By means of a broad spectrum of experimental techniques, the influence of different modifications on bio-based polymers such as blending with other components, copolymerization with different co-monomers and introduction of branching to yield complex architectures have been investigated. The present work on PHAs focused on the study of the dependence of polymer properties on both the fermentation process conditions (e.g. bacterial strain and carbon substrate used) and the method adopted to recover PHAs from cells. Furthermore, a solvent-free method using an enzyme and chemicals in an aqueous medium, was developed in order to recover PHAs from cells. Such a method allowed to recover PHA granules in their amorphous state, i.e. in native form useful for specific applications (e.g. paper coating). In addition, a commercial PHA was used as polymeric matrix to develop biodegradable and bio-based composites for food packaging applications. Biodegradable, non-toxic, food contact plasticizers and low cost, widely available lignocellulosic fibers (wheat straw fibers) were incorporated in such a polymeric matrix, in order to decrease PHA brittleness and the polymer cost, respectively. As concerns the study of polyamide 11, both the rheological and the solid-state behavior of PA11 star samples with different arm number and length was studied. Introduction of arms in a polymer molecule allows to modulate melt viscosity behavior which is advantageous for industrial applications. Also, several important solid-state properties, in particular mechanical properties, are affected by the presence of branching. Given the importance of using ‘green’ synthetic strategies in polymer chemistry, novel poly(-amino esters), synthesized via enzymatic-catalyzed polymerization, have also been investigated in this work.

Essays in Industrial Organization and Information Technology

Chapter 1 studies how consumers’ switching costs affect the pricing and profits of firms competing in two-sided markets such as Apple and Google in the smartphone market. When two-sided markets are dynamic – rather than merely static – I show that switching costs lower the first-period price if network externalities are strong, which is in contrast to what has been found in one-sided markets. By contrast, switching costs soften price competition in the initial period if network externalities are weak and consumers are more patient than the platforms. Moreover, an increase in switching costs on one side decreases the first-period price on the other side. Chapter 2 examines firms’ incentives to invest in local and flexible resources when demand is uncertain and correlated. I find that market power of the monopolist providing flexible resources distorts investment incentives, while competition mitigates them. The extent of improvement depends critically on demand correlation and the cost of capacity: under social optimum and monopoly, if the flexible resource is cheap, the relationship between investment and correlation is positive, and if it is costly, the relationship becomes negative; under duopoly, the relationship is positive. The analysis also sheds light on some policy discussions in markets such as cloud computing. Chapter 3 develops a theory of sequential investments in cybersecurity. The regulator can use safety standards and liability rules to increase security. I show that the joint use of an optimal standard and a full liability rule leads to underinvestment ex ante and overinvestment ex post. Instead, switching to a partial liability rule can correct the inefficiencies. This suggests that to improve security, the regulator should encourage not only firms, but also consumers to invest in security.

Industrial Restructuring in the Budapest agglomeration

The major 'motor' of the recent Hungarian industrial development has been foreign direct investment, particularly by multinational companies. This has stimulated the development process, as shown by the dynamism of production, exports and profitability of industry in Budapest. On the other hand, this has also led to a split of the industrial sphere into its foreign and domestic sections, or into foreign-owned companies and domestic SMEs. The major question asked in this project is where is Hungarian industry heading and will the gap between the contracting domestic part and the foreign multinationals continue to widen or will they be joined in a much more favourable scenario. Barta sees this as a question of whether Hungary can avoid the 'dead-end street' of South Asian industrialising countries, and instead move towards a new Eastern European or Hungarian model. He concludes that Budapest industry does not follow any given model and indeed its development probably cannot be seen as a 'model' proper in itself, but is, or will be, a mixture of different elements. This would be a welcome fusion of Hungary's rich human resources of accumulated knowledge with foreign direct investment. Budapest would play an exceptional role in such a process, as the gateway for foreign output to the rest of the country. The share of industry in the Budapest economy will continue to decrease, but it will become a more modern and profitable sector. It will also fulfil a technological transfer role between the developed world and the Hungarian countryside (or even a larger region of central and eastern Europe). Barta predicts that Budapest industry will develop a special structure, with a large subcontractor network supporting the large foreign enterprises, and alongside this industrial districts formed by SMEs.