Therapeutic drug monitoring of cefepime with micellar electrokinetic capillary chromatography: Assay improvement, quality assurance, and impact on patient drug levels

The improvement and performance of a micellar electrokinetic capillary chromatography assay for cefepime in human serum and plasma with a 50 μm id fused-silica capillary elongated from 40 to 60 cm is reported. Sample preparation with dodecylsulfate protein precipitation at pH 4.5, the pH 9.1 separation medium and the applied voltage were as reported previously[16]. The change resulted in a significant lower current, higher resolution and increased detection time intervals. The performance of the assay with multi-level internal calibration was assessed with calibration and control samples. Quality assurance data of a two year period assessed under the new conditions demonstrated the robustness of the assay. In serum samples of patients who received both cefepime and sulfamethoxazole, cefepime could not be detected due to the inseparability of the two compounds. The presence of an interference can be recognized by an increased peak width (width > 0.2 min), the appearance of a shoulder or an unresolved double peak. The patient data gathered during a three year period reveal that introduction of therapeutic drug monitoring led to a 50% reduction of the median drug level. The data suggest that therapeutic drug monitoring can help to minimize the risk of major adverse reactions and to increase drug safety on an individual basis. This article is protected by copyright. All rights reserved.

Recommendations for quality assurance programs in nuclear medicine facilities /

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Asbestos-containing materials in school buildings : bulk sample analysis quality assurance program : bulk sample rounds 16, 17 and 18 /

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Oil Shale Symposium : Sampling Analysis and Quality Assurance, March 1979 /

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Analytical procedures and quality assurance plan for the determination of xenobiotic chemical contaminants in fish.

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Analytical procedures and quality assurance plan for the determination of PCDD/PCDF in fish.

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Guide for preparation of quality assurance project plans for the National Estuarine Program

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Maintenance quality assurance : synthesis of measures /

"March 2005."

Quality assurance of chemical measurements /

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Quality assurance provisions for government agencies.

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Institutional meat purchase specifications : interim : quality assurance provisions approved by USDA.

"Effective date: October 1, 1990. Supersedes edition effective January 1988 and 1989. This 1992 reprint version includes all previous page changes."