Type III restriction-modification enzymes: a historical perspective

Restriction endonucleases interact with DNA at specific sites leading to cleavage of DNA. Bacterial DNA is protected from restriction endonuclease cleavage by modifying the DNA using a DNA methyltransferase. Based on their molecular structure, sequence recognition, cleavage position and cofactor requirements, restriction-modification (R-M) systems are classified into four groups. Type III R-M enzymes need to interact with two separate unmethylated DNA sequences in inversely repeated head-to-head orientations for efficient cleavage to occur at a defined location (25-27 bp downstream of one of the recognition sites). Like the Type I R-M enzymes, Type III R-M enzymes possess a sequence-specific ATPase activity for DNA cleavage. ATP hydrolysis is required for the long-distance communication between the sites before cleavage. Different models, based on 1D diffusion and/or 3D-DNA looping, exist to explain how the long-distance interaction between the two recognition sites takes place. Type III R-M systems are found in most sequenced bacteria. Genome sequencing of many pathogenic bacteria also shows the presence of a number of phase-variable Type III R-M systems, which play a role in virulence. A growing number of these enzymes are being subjected to biochemical and genetic studies, which, when combined with ongoing structural analyses, promise to provide details for mechanisms of DNA recognition and catalysis.

A theoretical perspective of the Telepinu Myth : archetypes and initiation rites in historical contexts

Abstract: Research on the Telepinu Myth (CTH 324) has shown that it is an inexhaustibly rich document and that therefore the different, and sometimes conflicting, lines of analysis need to be pursued further. In the light of the symbolic evidence presented by this mythological tradition, we propose to focus on the privileged position granted to spatial symbols and to hypothesize the function this Myth served in specific contexts of the Hittite history, characterized by the increase and reduction of lands under Hittite jurisdiction. In this regard, we propose to evaluate the symbolic function that the Telepinu Myth displayed in order to rebuild the sacred space of the territories governed by the Hittites, when they were undergoing changes brought about by increased political and military contact with neighboring societies.

The degree of predictability of earthquakes in several regions of China: statistical analysis of historical data

The stress release model, a stochastic version of the elastic-rebound theory, is applied to the historical earthquake data from three strong earthquake-prone regions of China, including North China, Southwest China, and the Taiwan seismic regions. The results show that the seismicity along a plate boundary (Taiwan) is more active than in intraplate regions (North and Southwest China). The degree of predictability or regularity of seismic events in these seismic regions, based on both the Akaike information criterion (AIC) and fitted sensitivity parameters, follows the order Taiwan, Southwest China, and North China, which is further identified by numerical simulations. (c) 2004 Elsevier Ltd. All rights reserved.

O exame da admissibilidade financeira e orçamentária de proposições na Assembleia Nacional da França

Analisa o controle da admissibilidade financeira de proposições legislativas, com impacto nas contas públicas na Assembleia Nacional da França. Compara, subsidiariamente, com o caso brasileiro referente à Câmara dos Deputados. Expõe a atuação das diferentes instituições do Estado francês envolvidas no exame de admissibilidade financeira. Investiga as competências e o processo legislativo relacionados, especificamente no que tange à Assembleia Nacional. Detalha os critérios e os conceitos relativos ao exame, adotados por aquela casa legislativa e consigna a evolução histórica recente dos principais aspectos do controle de admissibilidade na França.

Model of sources: a proposal for the hierarchy, merging strategy and representation of the information sources in virtual models of historical buildings

Contributed to: Fusion of Cultures: XXXVIII Annual Conference on Computer Applications and Quantitative Methods in Archaeology – CAA2010 (Granada, Spain, Apr 6-9, 2010)

"A casa dos ímpios se desfará, mas a tenda dos retos florescerá" : a participação da Frente Parlamentar Evangélica no legislativo brasileiro

Analisa, a partir do trabalho de campo empreendido nas atividades da Frente Parlamentar Evangélica do Congresso Nacional, como estes parlamentares participam de tempos e espaços do legislativo. Deste modo, trago a descrição etnográfica os discursos destes deputados religiosos que denotam, mormente, uma "guerra santa" entre os "ungidos" e o Inimigo que ocupa o mundo da política e o espaço público brasileiro. Assim, suspeito que presenciamos contemporaneamente uma maior dessectarização de grupos evangélicos na política quando estes agentes procuram não apenas se tornar a Igreja do amanhã (concorrendo com a Igreja Católica pela posição de religião civil). Mas, sobretudo, tornar, através da participação na política, o mundo dos ímpios o mundo dos justos. Ora, considero, por isso mesmo, as formas particulares de aquisição de sutilezas e de competências necessárias a fim de profissionalizar o político e as relações políticas nas quais estes políticos se engajam. Deste modo, os evangélicos também têm que aprender tais habilidades especialmente por que estão no processo de construção de uma herança histórica na política. Por fim, analiso como os evangélicos, enquanto políticos profissionais, agenciam uma forma de representação e participação políticas que considera tanto o exercício cotidiano da fé quanto uma espécie de investimento nas atividades do século, reinterpretando, assim, o histórico processo de secularização do Estado.

Limnology in Argentina: an historical account.

This short review includes major books, articles and episodes concerned with studies on inland waters that have affected the development of limnology in Argentina.

Multiple table factor analysis applied to the higher education competences

Due to the recent implantation of the Bologna process, the definition of competences in Higher Education is an important matter that deserves special attention and requires a detailed analysis. For that reason, we study the importance given to severa! competences for the professional activity and the degree to which these competences have been achieved through the received education. The answers include also competences observed in two periods of time given by individuals of multiple characteristics. In this context and in order to obtain synthesized results, we propose the use of Multiple Table Factor Analysis. Through this analysis, individuals are described by severa! groups, showing the most important variability factors of the individuals and allowing the analysis of the common structure ofthe different data tables. The obtained results will allow us finding out the existence or absence of a common structure in the answers of the various data tables, knowing which competences have similar answer structure in the groups of variables, as well as characterizing those answers through the individuals.

Structure and Historical Changes in the Groundfish Complex of the Eastern Bering Sea

The eastern Bering Sea is a major marine ecosystem containing some of the largest populations of groundfish, crabs, birds, and marine mammals in the world. Commercial catches of groundfish in this region have averaged about 1.6 million tons (t) annually in 1970-86. This report describes the species and relative importance of species in the eastern Bering Sea groundfish complex, the environment in which they live, and the history of the fisheries and management during the years 1954 - 1985. Historical changes in abundance and the condition of the principal species at the end of this first 30 years of exploitation are also examined. Results suggest that the biomass of the groundfish complex is characterized by variability rather than stability. The most reliable data (1979 to 1985) suggests that the biomass of the complex fluctuated between 11.8 and 15.7 million t. Even greater variability is suggested by the less reliable data from earlier years. Because of its dominance in the complex and wide fluctuations in abundance, walleye pollock (Theragra chalcogramma) is primarily responsible for the major variations in abundance of the complex. After 30 years of exploitation, the complex was generally in excellent condition. (PDF file contains 100 pages.)

Regulation in Uganda's Lake Victoria fishery: historical and contemporary conditions

This paper sets out to explore how Uganda's lake Victoria fishery has been managed. It explores the management of the fishery during the protectorate period, and argues that the apparent success of regulation during this time may be attributed to the very heightened controls arising from Sleeping Sickness Controls. Once these were removed, entry into the fishery was rapid and uncontrolled, and the resultant impact on fish stocks was quickly felt. With its huge area, considerable shoreline, and innumerable islands, the lake Victoria fisheries service was quickly overwhelmed and disbanded as a result. In the early independence years, the Republic's government focused on developing the fishery, plans thwarted by turmoil of, and following, Idi Amin's reign. More recently, the fishery has prospered from Uganda's entry into the Nile perch fillet export market, which ahs adversely affected stocks. We present and comment on recently collected data that considers fishers' impressions of the status of the fishery, regulations and future managerial possibilities, and comment on these in the light of recent changes to Uganda's fisheries administration