Rapid mass spectrometric peptide sequencing and mass matching for characterization of human melanoma proteins isolated by two-dimensional PAGE.

We report a general mass spectrometric approach for the rapid identification and characterization of proteins isolated by preparative two-dimensional polyacrylamide gel electrophoresis. This method possesses the inherent power to detect and structurally characterize covalent modifications. Absolute sensitivities of matrix-assisted laser desorption ionization and high-energy collision-induced dissociation tandem mass spectrometry are exploited to determine the mass and sequence of subpicomole sample quantities of tryptic peptides. These data permit mass matching and sequence homology searching of computerized peptide mass and protein sequence data bases for known proteins and design of oligonucleotide probes for cloning unknown proteins. We have identified 11 proteins in lysates of human A375 melanoma cells, including: alpha-enolase, cytokeratin, stathmin, protein disulfide isomerase, tropomyosin, Cu/Zn superoxide dismutase, nucleoside diphosphate kinase A, galaptin, and triosephosphate isomerase. We have characterized several posttranslational modifications and chemical modifications that may result from electrophoresis or subsequent sample processing steps. Detection of comigrating and covalently modified proteins illustrates the necessity of peptide sequencing and the advantages of tandem mass spectrometry to reliably and unambiguously establish the identity of each protein. This technology paves the way for studies of cell-type dependent gene expression and studies of large suites of cellular proteins with unprecedented speed and rigor to provide information complementary to the ongoing Human Genome Project.

Receipts for room and board, 1796-1798

Eight receipts from Abiel Holmes for room and board dated October 19, 1796; January 3, 1797; February 20, 1797; April 19, 1797; July 14, 1797; October 10, 1797; April 17, 1798; and July 12, 1798.

Receipt for medical services, 1799 September

Two copies of a handwritten receipt signed by Dr. William Gamage (Harvard AB 1767) for "medicine and attendance" for Loammi Baldwin's son in September 1799.

Records of the Debating Club of Resident Graduates, 1792-1793

This journal contains minutes from meetings held from February 1792 through October 1793. These minutes include the names of participants and the questions and arguments which were debated, including: whether or not French slaves in the West Indies should be emancipated; whether or not reading novels was beneficial; whether sermons were more effective when memorized than when simply read; whether theater contributed to corrupt morals; whether drunkenness or gambling was more detrimental to society; and whether or not French assistance to the colonies in their Revolutionary War provided sufficient cause for the United States to join with France in its own wars. Most of the topics of debate centered on religion, government and education. Several entries also include notes on related topics of discussion, including the reasons for Native American tribes' hostilities against federal authorities, and there are several references to published works which were cited and consulted in the course of debate.

Morgan horses /

Reprint of chapters V to VII of D.C. Linsley's Morgan horses.

The duty of Columbia College to the community, and its right to exclude Unitarians from its professorships of physical science,

Signed: Samuel B. Ruggles.

Shakers.

"The Legislature of New Hampshire and their constitutuion": p. 4-7; "Minority report of the Judiciary Committee, upon the petition of Franklin Munroe and others in relation the the Society of Christians called Shakers": p. 7-12.

Report of the Governor's Commission for the Study of Problems in the Education of Negroes in North Carolina.

N.C. Newbold, chairman of the commission.

The Madonna in legend and history

Mode of access: Internet.

Igrot Shadal /

Personal letters of Luzzatto in Hebrew.

An address, delivered in Utica, before the Sunday School Societies, on the fifty-first anniversary of American independence /

Cover title: Mr. Aikin's address on the political influence of Sabbath schools, July 4, 1827.

The maintenance of peace;

Mode of access: Internet.

Papers

Papers published chiefly at the University of California between 1913-1924.

Romantic biography of the age of Elizabeth; or, Sketches of life from the bye-ways of history.

v. 1. Introduction. Margaret of Valois, Queen of Henry IV. Robert Dudley, Earl of Leicester. Castelnau, Ambassador from France. La Mothe Fenelon. La Mothe Fenelon and Castelnau. Thomas Howard, fourth duke of Norfolk. Hugh, Earl of Tyrone, and notices of Walter, 1st Earl of Essex. Dr. Dee.--v. 2. Calvin and the church of Geneva. William Whittingham and the Puritans. Archbishop Whitgift and Dr. Cartwright. John Darrel, the exorcist. Loyola and the order of the Jesuits. Robert Parsons, Edmund Campian, and the Jesuits in England. Pope Sixtus V. Charles de Valois, Duc d'Angoulême. Henry de la Tour d'Auvergne, Viscount Turenne and Duke de Bouillon.

Reprints and papers on parasites.

Made-up set; binder's title.