Echicetin coated polystyrene beads: a novel tool to investigate GPIb-specific platelet activation and aggregation.

von Willebrand factor/ristocetin (vWF/R) induces GPIb-dependent platelet agglutination and activation of αIIbβ3 integrin, which also binds vWF. These conditions make it difficult to investigate GPIb-specific signaling pathways in washed platelets. Here, we investigated the specific mechanisms of GPIb signaling using echicetin-coated polystyrene beads, which specifically activate GPIb. We compared platelet activation induced by echicetin beads to vWF/R. Human platelets were stimulated with polystyrene beads coated with increasing amounts of echicetin and platelet activation by echicetin beads was then investigated to reveal GPIb specific signaling. Echicetin beads induced αIIbβ3-dependent aggregation of washed platelets, while under the same conditions vWF/R treatment led only to αIIbβ3-independent platelet agglutination. The average distance between the echicetin molecules on the polystyrene beads must be less than 7 nm for full platelet activation, while the total amount of echicetin used for activation is not critical. Echicetin beads induced strong phosphorylation of several proteins including p38, ERK and PKB. Synergistic signaling via P2Y12 and thromboxane receptor through secreted ADP and TxA2, respectively, were important for echicetin bead triggered platelet activation. Activation of PKG by the NO/sGC/cGMP pathway inhibited echicetin bead-induced platelet aggregation. Echicetin-coated beads are powerful and reliable tools to study signaling in human platelets activated solely via GPIb and GPIb-triggered pathways.

WebGIS: Anwendungen über ArcGIS Server in der Kantonalen Verwaltung Luzern

Eine immer größere Zahl von Mitarbeitern öffentlicher Verwaltungen arbeitet direkt oder indirekt mit Geodaten. Nicht alle sind Spezialisten im Umgang mit GIS Software. ArcGIS Server bietet mit einem umfangreichen Framework die Möglichkeit, angepasste GIS Anwendungen zu entwickeln, den Funktionsumfang auf benötigte Funktionen zu reduzieren und komplexe Arbeitsabläufe zu optimieren. Die Abteilung Geoinformation und Vermessung des Kantons Luzern entwickelt seit dem Jahr 2006 in Zusammenarbeit mit der Universität Bern ArcGIS Server-basierte Webanwendungen für verschiedene Abteilungen der kantonalen Verwaltung. In dieser Zeit sind mehrere Anwendungen entstanden, darunter eine Webapplikation für die effiziente Erfassung, Beurteilung und Verwaltung von Waldeingriffsflächen (Waldportal), für die dynamische Abgrenzung und Auswertung von Einzugsgebieten, sowie für das Betrachten von aufgezeichneten Videos von Kantonsstraßenabschnitten. In der Präsentation werden die genannten Applikationen vorgestellt und Hintergründe der Entwicklung sowie der Architektur besprochen.

On the leakage-power modeling for optimal server operation

Leakage power consumption is a com- ponent of the total power consumption in data cen- ters that is not traditionally considered in the set- point temperature of the room. However, the effect of this power component, increased with temperature, can determine the savings associated with the careful management of the cooling system, as well as the re- liability of the system. The work presented in this paper detects the need of addressing leakage power in order to achieve substantial savings in the energy consumption of servers. In particular, our work shows that, by a careful detection and management of two working regions (low and high impact of thermal- dependent leakage), energy consumption of the data- center can be optimized by a reduction of the cooling budget.

Leakage and temperature aware server control for improving energy efficiency in data centers

Reducing the energy consumption for computation and cooling in servers is a major challenge considering the data center energy costs today. To ensure energy-efficient operation of servers in data centers, the relationship among computa- tional power, temperature, leakage, and cooling power needs to be analyzed. By means of an innovative setup that enables monitoring and controlling the computing and cooling power consumption separately on a commercial enterprise server, this paper studies temperature-leakage-energy tradeoffs, obtaining an empirical model for the leakage component. Using this model, we design a controller that continuously seeks and settles at the optimal fan speed to minimize the energy consumption for a given workload. We run a customized dynamic load-synthesis tool to stress the system. Our proposed cooling controller achieves up to 9% energy savings and 30W reduction in peak power in comparison to the default cooling control scheme.

Server power modeling for run-time energy optimization of cloud computing facilities

As advanced Cloud services are becoming mainstream, the contribution of data centers in the overall power consumption of modern cities is growing dramatically. The average consumption of a single data center is equivalent to the energy consumption of 25.000 households. Modeling the power consumption for these infrastructures is crucial to anticipate the effects of aggressive optimization policies, but accurate and fast power modeling is a complex challenge for high-end servers not yet satisfied by analytical approaches. This work proposes an automatic method, based on Multi-Objective Particle Swarm Optimization, for the identification of power models of enterprise servers in Cloud data centers. Our approach, as opposed to previous procedures, does not only consider the workload consolidation for deriving the power model, but also incorporates other non traditional factors like the static power consumption and its dependence with temperature. Our experimental results shows that we reach slightly better models than classical approaches, but simul- taneously simplifying the power model structure and thus the numbers of sensors needed, which is very promising for a short-term energy prediction. This work, validated with real Cloud applications, broadens the possibilities to derive efficient energy saving techniques for Cloud facilities.

Leakage-aware cooling management for improving server energy efficiency

The computational and cooling power demands of enterprise servers are increasing at an unsustainable rate. Understanding the relationship between computational power, temperature, leakage, and cooling power is crucial to enable energy-efficient operation at the server and data center levels. This paper develops empirical models to estimate the contributions of static and dynamic power consumption in enterprise servers for a wide range of workloads, and analyzes the interactions between temperature, leakage, and cooling power for various workload allocation policies. We propose a cooling management policy that minimizes the server energy consumption by setting the optimum fan speed during runtime. Our experimental results on a presently shipping enterprise server demonstrate that including leakage awareness in workload and cooling management provides additional energy savings without any impact on performance.

Gene2EST: a BLAST2 server for searching expressed sequence tag (EST) databases with eukaryotic gene-sized queries

Expressed sequence tags (ESTs) are randomly sequenced cDNA clones. Currently, nearly 3 million human and 2 million mouse ESTs provide valuable resources that enable researchers to investigate the products of gene expression. The EST databases have proven to be useful tools for detecting homologous genes, for exon mapping, revealing differential splicing, etc. With the increasing availability of large amounts of poorly characterised eukaryotic (notably human) genomic sequence, ESTs have now become a vital tool for gene identification, sometimes yielding the only unambiguous evidence for the existence of a gene expression product. However, BLAST-based Web servers available to the general user have not kept pace with these developments and do not provide appropriate tools for querying EST databases with large highly spliced genes, often spanning 50 000–100 000 bases or more. Here we describe Gene2EST (http://woody.embl-heidelberg.de/gene2est/), a server that brings together a set of tools enabling efficient retrieval of ESTs matching large DNA queries and their subsequent analysis. RepeatMasker is used to mask dispersed repetitive sequences (such as Alu elements) in the query, BLAST2 for searching EST databases and Artemis for graphical display of the findings. Gene2EST combines these components into a Web resource targeted at the researcher who wishes to study one or a few genes to a high level of detail.

The MetaFam Server: a comprehensive protein family resource

MetaFam is a comprehensive relational database of protein family information. This web-accessible resource integrates data from several primary sequence and secondary protein family databases. By pooling together the information from these disparate sources, MetaFam is able to provide the most complete protein family sets available. Users are able to explore the interrelationships among these primary and secondary databases using a powerful graphical visualization tool, MetaFamView. Additionally, users can identify corresponding sequence entries among the sequence databases, obtain a quick summary of corresponding families (and their sequence members) among the family databases, and even attempt to classify their own unassigned sequences. Hypertext links to the appropriate source databases are provided at every level of navigation. Global family database statistics and information are also provided. Public access to the data is available at http://metafam.ahc.umn.edu/.

SQL Server Management Studio Express Instalación

Instalación de SQL Server Management Studio Express en un MS Windows 7 Profesional.

SQL Server Management Studio Express 01

Primeros pasos en el uso de este programa cliente de acceso y administración de SQL Server. Este cliente, en su versión Express, es gratuito. El ejemplo del vídeo se conecta con un servidor remoto SQL Server 2008 R2.

SQL Server Management Studio Express 02

Segunda parte de los primeros pasos en el uso de este programa cliente de acceso y administración de SQL Server. Este cliente, en su versión Express, es gratuito. El ejemplo del vídeo se conecta con un servidor remoto SQL Server 2008 R2. Modificar tablas. Exportar definiciones del esquema. Resultados a cuadrícula o texto. Guardar resultados.

Transacciones en SQL Server

Una introducción a los objetivos y contenidos de la sesión 2 del curso de Bases de Datos dentro del Máster Universitario en Desarrollo de Aplicaciones y Servicios Web de la Universidad de Alicante. Se describe someramente qué se entiende por una transacción y se nombran los niveles de aislamiento en SQL Server. Todo enfocado a la realización de los ejercicios-demostraciones de esos niveles de aislamiento. Ingenuo y con fallos de encuadre e iluminación clamorosos, pero no tiene más ambiciones que la de dejar constancia de algunas de las cosas que se dijeron en clase.

Ejemplos de transacciones en SQL Server

Descripción de cómo facilitar la ejecución de los ejercicios de la sesión de transacciones del moodle de la asignatura. Se trata de reorganizar las ventanas y hacer espacio para tener dos consultas a la vista. En cada una de ellas se mantendrán una o varias transacciones que pretendemos ejecutar simultáneamente. De esta forma podremos ver qué transacciones esperan a las otras y pasar de una a otra consulta con facilidad.

Paginación en SQL Server

Una introducción a los objetivos y contenidos de la sesión 3 del curso de Bases de Datos dentro del Máster Universitario en Desarrollo de Aplicaciones y Servicios Web de la Universidad de Alicante. El trabajo que permite la calificación de esta primera parte del curso se basa, precisamente, en lo que se describe en este vídeo. La paginación de resultados es una técnica que pretende agilizar el manejo remoto de grandes cantidades de datos. El ejemplo clásico es la navegación por un catálogo de productos. Si es el servidor el que, por el procedimiento que sea, divide la lista completa en páginas y solo envía una de ellas al cliente, estamos ahorrando en tiempo y uso de red. En SQL Server, para esta tarea, es fácil encontrar opiniones que desaconsejan el uso de cursores puesto que se supone que el entorno objetivo es de alta concurrencia. No obstante, se puede usar TOP() y ROW_NUMBER(). La sesión consiste en una serie de ejemplos sobre todas estas herramientas.