Influence of stocking density on the culture potential of freshwater catfish Pangasius pangasius in pond

Influence of stocking density on the production of freshwater catfish Pangasius pangasius with formulated feed in ponds was studied. The fish fry were collected from the Meghna river near Chandpur which is a natural breeding ground of this fish. Three stocking densities chosen during this experiment were 5000, 8000 and 11000 fry/ha with an initial weight of 42.57 ±2.51 g. The formulated feed was prepared in the laboratory which contained 45% fish meal, 30% mustard oil cake, 15% wheat bran and 10% rice bran to supply 40.48% protein in feed. The growth of P. pangasius in terms of net weight gain was 409.49, 266.49 and 236.37 g at the 5000/ha, 8000/ha and 11000/ha stocking densities respectively, which was statistically significant (p<0.05). High food conversion ratio (FCR) was also observed during this research study which ranged between 7.06 to 7.72. A production of 2.6 tons/ha at the stocking density of 11000/ha; 2.13 tons/ha at the stocking density of 8000/ha and 2.04 tons/ha at the stocking density of 5000/ha was obtained, while the growth of individual fish at the end of experiment was in opposite order. The result of this experiment indicates that stocking density has significant influence on the culture potential of freshwater catfish P.pangasius.

Development of low cost feed using local ingredients for culture of freshwater prawn, Macrobrachium rosenbergii in ponds

Two experiments were conducted to formulate and evaluate test diets using locally available ingredients to find out suitable diets for mono and polyculture of Macrobrachium rosenbergii in ponds. The first experiment was conducted from 1 July 2003 to 29 September 2003 in 12 experimental ponds each measuring 30 square meters behind the Fisheries Faculty Building, BAU campus, Mymensingh. Three experimental diets containing 30% protein were formulated using fish meal, meat and bone meal, mustard oilcake, sesame meal and rice bran and assigned to treatments 1, 2 and 3, respectively. A special shrimp feed (Starter-II) from Saudi-Bangla Fish Feed Ltd. was assigned to treatment 4 (Control). Each treatment had three replications. Juveniles of M. rosenbergii (2.90±0.81g) were stocked at the rate of 4/square meter. Prawns were fed three times daily at the rate of 15% of their body weight at the beginning, which was gradually reduced to 10% and 5% for the last two months. There was no significant (P>0.05) difference between the weight gains of prawns fed diets 1 and 4 (control), but they were significantly (P<0.05) higher than those of diets 2 and 3. The FCR values of diets ranged between 2.61 to 3.36 with diets 1 and 4 showing significantly (P<0.05) lower FCR values. The survival rate of prawns ranged between 68 to 78% with prawns fed diets 1 and 4 showing significantly higher survival rate. The production of prawn ranged from 921 to 1,428 kg/ha/90 days and diet 1 gave the highest production. Treatment 1 gave the highest net profit ofTk. 161,980/ha/90 days. The second experiment was conducted from December, 2003 to April, 2004 to see the growth of over wintered M. rosenbergii juveniles in polyculture with indigenous major carps (catla and rohu) in ponds using formulated diets. Three isoenergetic experimental diets formulated using fish meal, meat and bone meal, mustard oil cake, rice bran, wheat bran and molasses, and a shrimp feed 'Golda special feed' from Saudi-Bangla Fish Feed Ltd. were assigned to treatments 1, 2, 3 and 4 (control), respectively, each treatment had two replications. Eight experimental ponds each measuring 80 square meters in the Field Laboratory Complex of the Faculty of Fisheries, BAU campus, Mymensingh were used. The mean initial weights of M. rosenbergii, catla and rohu were 1.60±0.01, 30.0±0.09 and 25.0±0.08 g, respectively. A total of 160 fish and prawn (20,000/ha) were stocked in each pond at the ratio of 2:1:1 (prawn: catla: rohu). Fish were fed at the rate of 3-5% of their body weight. Prawns fed diet 1, 2 and 4 showed higher weight gains compared to diet 3. The weight gain of catla was significantly higher in T1 while in case of rohu was higher in T1 and T4, respectively. There were no significant differences (P>0.05) in the survival rate of fish as obtained from different treatments. The overall total fish production ranged from 2196 to 2679 kg/ha/5 months. The highest production and the highest profit (Tk. 56,531/ha/5 months) was obtained from T1 and the lowest (Tk. 24,932/ha/5 months) from T4.

Effect of feeding frequency on the growth of common carp (Cyprinus carpio L.) fry

A laboratory-feeding trail was conducted for 45 days with fry of common carp Cyprinus carpio L. (0.45±0.03g) in aquaria in a static indoor fish rearing system. The fry were fed on a pelleted diet containing 33% crude protein having fishmeal as major protein source. The fish fry in five treatments A, B, C, D, and E, each with two replicates were fed on 5% daily ration divided into different feeding frequencies of 2, 3, 4, 5 and 6 times a day respectively in order to observe the growth performance. Each replicate contained 15 fry having total initial weight of 6.87±0.31g. At the end of the feeding trial, significantly different and higher (p<0.05) growth response was observed in treatment C having a feeding frequencies of 4 times a day. Significantly the highest and the lowest percent growth of 334.30 and 218.91% were observed in fish fed on the diet (Treatment C) with 4 times and (Treatment A) 2 times feeding frequencies per day, respectively. Food conversion ratio (FCR) of 1.78 was significantly higher (p

Input-output relationship and economics of pangas monoculture and carp-pangas polyculture in two districts of Bangladesh

An attempt was made to study the input-output relationships and economics of pangas monoculture and carp-pangas polyculture in Bangladesh. By analyzing the data collected from 50 pangas farms and 55 carp-pangas farms, the study has investigated the production systems of two technologies and the effects of fingerling stocking and applications of feed and fertilizer on fisheries income. The data were collected from the fishermen of Trishal and Bhaluka of Mymensingh district, and Kahaloo and Adamdighee of Bogra district during 2001-02. For pangas monoculture, the stocking density was 31,561 per ha while it was 55,017 per ha in carp-pangas polyculture. Most of the farmers used urea, TSP and lime before stocking. Rice and wheat bran happened to be the most common feed ingredients for both types of culture in general. Other important ingredients used were mustard oil-cakes, rice polish, wheat flour, fish meal, bone meal, soybean meal and poultry litter. In terms of quantities, rice bran and wheat bran dominated the farmers list. Rice and wheat bran together constituted about 60% of all studied feeds. Feed cost constituted 59.13% of total costs for pangas monoculture and 67.44% for carp-pangas polyculture. Per ha productions of pangas and carp-pangas in a single culture cycle were 15,508 kg and 19,745 kg, respectively. Per ha gross profits were estimated to be Tk 310,311 and Tk 464,418 for pangas monoculture and carp-pangas polyculture, respectively. Net profit appeared to be Tk 264,216 per ha for pangas monoculture and Tk 416,509 per ha for carp-pangas polyculture. The BCRs calculated were 1.46 and 1.68 for monoculture and polyculture, respectively. The break-even costs per kg of fish were estimated at Tk 36.93 for pangas and Tk 30.93 for mixed species which was much lower than the prices the producers received. Break-even productions were estimated at 10,702 kg per ha for pangas monoculture and 11,784 kg per ha for carp-pangas polyculture. Fingerling and feed cost, and pond size significantly explained the variation of income from pangas monoculture. These factors have significantly influenced the income from the crop. Functional analysis shows that 1% increase in the feed cost might increase 0.51% of pangas income and 0.41% in carp-pangas income. No other inputs had shown this much of responses to increasing income from a fish.

HACCP based management system for improving quality of freshwater giant prawn, Macrobrachium rosenbergii

The study was conducted on the present status of HACCP based quality management system of golda, Macrobrachium rosenbergii farms in Fulpur region of Mymensingh. Information was collected on general condition of farms, culture systems and post-harvest quality management. In almost all farms, there is no or inadequate infrastructure facilities such as, road access, electric supply, telecommunications, ice, feed storage facility, vehicle for golda transportation, washing and toilet facilities. The problems associated with sanitation and hygiene was: widespread use of cow dung, poultry manure and construction of open toilet within the vicinity of prawn culture pond. Different grades of commercially available and locally prepared feeds were used for golda culture in the pond. Golda post-larvae (PL) of 40-50 days old were stocked with carp species. The price of golda PL ranged from Tk. 1.00 to Tk. 1.25/piece. The pond size varied from 50 decimal (0.2 ha) to 2.5 acre (1.0 ha) with an average depth of 2-2.5 m. The culture period of golda varied from April-May to November-December and survival rate ranged between 75 and 80%. Production of golda varied from 250-500 kg/acre (625-1,250 kg/ha). Harvested golda were transported to city market within 4 h. Two size grading were generally followed during pricing, e.g. Tk. 500 to 550/kg for >100 g size and Tk. 300/kg for <100 g size. The cost-benefit ratio was found to remain around 1:1.25 depending on availability of PL. Water quality parameters such as, water temperature, pH, dissolved oxygen, total alkalinity and chlorophyll a in five golda farms in Fulpur region were monitored. Water temperature ranged from 29°C to 33°C, dissolved oxygen from 2.28 to 4.13 mg/l, pH between 6.65 and 7.94, alkalinity from 44 to 70 mg/l and chlorophyll a concentration from 61.88 to 102.34 µg/l in the five investigated ponds. The Aerobic Plate Count (APC) of the water sample was within the range of 2.0x10^6 - 2.96x10^7 CFU/ml and of soil samples within the range of 6.9x10^6 - 7.73x10^6 CFU/g. Streptococcus sp., Bacillus sp., Escherichia coli, Staphylococcus sp., Pseudomonas sp. and Salmonella sp. were isolated from pond water and sediment. Different feed samples used for golda was analyzed for proximate composition. Moisture content ranged around 14.14-21.22%, crude protein 20.55-44.1%, lipid 4.67-12.54% and ash 9.7-27.69%. The TVB-N values and peroxide values of feeds used as starter, grower and fish meal were found within the acceptable ranges and samples were free from pathogenic organisms. A training was organized for the golda farmers on HACCP, water quality and post-harvest quality management of prawn.

Utilization of frog waste

Commercial frog waste samples have been converted into meals by cooking at 0.7 kg/sq. cm for 30 min, draining off the stick water and drying the press cake either in the sun, tunnel dryer under controlled conditions or hot air oven. Yield of the meal varied between 18.6 to 21.5% of the fresh frog waste. Chemical analyses of the meals have shown that the meals prepared from frog waste conform to standards prescribed for fish meal and livestock feed and can therefore be used for supplementation of poultry/animal feed.

Formulation, stability and keeping quality of three pelleted feeds used in carp culture

Three dry pelleted feeds incorporating fish meal, fish silage or a mixture of colocasia leaf powder and fish meal were formulated for use in carp culture. The diets formulated were tested for water stability and also for changes in their quality parameters over storage of three months. The different pellets showed satisfactory water stability. The variations recorded in the proximate composition during the period of storage did not bring about any drastic change in the overall keeping quality of the feeds. Therefore, the three formulated feeds are considered suitable for use in the culture of carps.

The effect of various protein levels on the growth and survival rates of Penaeus monodon Fabricius

Postlarvae of tiger prawns, P. monodon , were fed with various protein levels of 0; 30; 40 and 50%, fish meal and rice bran were combined and pelletized for 16 wks. Prawns used had an average mean length of 1.2 mm and an average mean weight of 5.5 mg. Nitrite, ammonia, dissolved oxygen content, temperature and pH were monitored. After one month of feeding, growth increments of postlarvae fed with 30% protein diet were significantly different from those given 0; 40 and 50%. Survival rate of the group fed 40% protein was higher (81.7%) but was not significantly different from those fed 30% (68.0%). The highest feed efficiency was obtained from the 30% protein pellets (4.6). At the end of 16 weeks, growth increment of 40% protein level was significantly different from the 30% and 50% protein levels, but the survival rate was not signficantly different from the 30% protein level. Feed conversion for the 40% protein diet was 2.8.

Preliminary chemical and physical evaluation of some formulated feeds for P. monodon

The culture of Penaeus monodon has explicitly defined the need for diet formulations or supplementary feeds that would promote optimum growth and survival of the animal. A total of 28 feed combinations were developed for P. monodon. Fish meal, shrimp head meal, squid head meal, Ascetes spp. rice bran, and soybean cake were used as primary ingredients in these feeds. The commercial vitamin mix No. 22 was added to the dry ingredients. Gelatinized corn starch and wheat flour were used as binders. The pellets were extruded using a portable kitchen grinder with a diameter of 4 mm. The products were either sun-dried for 8 hours or oven-dried overnight at 50 degree C to stabilize moisture at 8-10%. The pellets were then kept in covered glass bottles and stored in the laboratory at room temperature. The cost of the feeds excluding labour were also computed. The pellets were analyzed for protein, fat, carbohydrate, crude fiber, ash, and moisture contents using standard procedures. They were also analyzed for water stability. To test the stability of pellets in water, 2-g samples were placed in plankton nets (mesh #40) and suspended in water for two, and six hours. The undissolved samples were then vacuum-dried and the moisture determined. Cost of the feeds ranged from P1.10 to P2.60 per kg depending on the feed ingredient. Squid and Ascetes spp. were rather expensive for use as basic ingredients. Proximate analysis of dry weight showed percentage protein content ranged from 20-63 g; fat, 8-20 g; carbohydrate (by difference), 11-36 g; ash, 8-28 g; moisture, 6-11 g; and crude fiber, 5 . 13 g. Stability tests showed that after two hours, 35-88% of solids remained intact and after 6 hours, 20-55% of the pellets remained undissolved. When a pellet disintegrates easily, pollution of the water occurs. Chances for the shrimp to feed on the pellet is minimized when the pellet is unstable. Thus, the search for a more compact feed pellet has to be continued.

Preliminary biological evaluation of some formulated feeds for P. monodon

Rice bran is widely used by fish farmers as supplementary feed while soybean cake is used both as feed and as fertilizer in fishponds. Both fish meal and shrimp head have been found acceptable as feed ingredients. However, not much is known of the acceptability and efficiency of a mixture of these ingredients as feed for Penaeus monodon larvae. Ninety 127-day old P. monodon were measured for length and weight and were randomly divided into nine aquaria each containing 20 liters of water. These were fed 'lampirong' for two months previous to the study. There were three replications for each treatment. Length, weight, and survival rates were used to compare the efficiency of the diets. Weighed amounts of pellets equivalent to 100% of the body weight were fed during the first three days and reduced to 50% thereafter. A stopwatch was used to determine the length of time that elapsed before the shrimps would approach the pellet. Ten shrimps approximately 4 months in age were placed in 10 liters of water in a 25-liter aquarium. Two grams of each pellet type were placed simultaneously on opposite sides of the aquarium. The time that elapsed from the moment the pellets sunk to the bottom up to the time that any one shrimp approached the pellets was recorded. The group fed the imported pellets gained the most. Those fed FP-2s-77 elongated faster than those fed FP-1s-77. Survival rate of those fed FP-2s-77 was 37% while those fed imported pellets was 73%. Both 1s and 2s pellets disintegrated in water easily but the imported pellets were stable even after six hours in water. The attractability test for the pellets showed that the prawns were more readily attracted to the pellets 1s and 2s than to the imported pellets.

Ipil-ipil (Leucaena leucocephala) leaves as a plant protein source in prawn diets

Penaeus monodon juveniles were fed diets containing fish meal, shrimp head meal and ipil-ipil leaves soaked and unsoaked, local and peruvian varieties. Mean weight gain at the end of 8 wk was significantly highest among those given the diet containing commercial ipil-ipil leaves. Gain in length followed the same pattern as mean weight gains. Among the diets containing ipil-ipil leaves there was a direct relationship in the amount of mimosine in the diet and the survival rate, the lower the amount of mimosine (due to soaking) the higher the survival rate. The Results thus indicate the beneficial effect of the addition of commercial ipil-ipil leaves to the diets of prawns, providing the mimosine content is kept low by soaking. A reduce in costs is also obtained, since 1kg of shrimp head meal or fish costs more than 2 or 4 tons, respectively, than that of ipil-ipil foliage.

Effect of varying crude protein levels on spawning frequency and growth of Sarotherodon niloticus breeders

Four-month-old S. niloticus breeders were fed with dry pellets containing 20-50% crude protein and the frequency of spawning involving removal of egg from the mouthbrooding females and growth were determined. When the diets contain high quality proteins from fish meal and soybean oil meal and the amounts of daily food allowance are at satiation level, the influence of increasing dietary crude protein on spawning frequency involving egg removal from the brooder and growth may not be significant.

The effect of various protein levels on the growth and survival rates of Penaeus monodon Fabricius

Postlarvae of tiger prawns, P. monodon, were fed with various protein levels of 0; 30; 40 and 50%, fish meal and rice bran were combined and pelletized for 16 wks. Prawns used had an average mean length of 1.2 mm and an average mean weight of 5.5 mg. Nitrite, ammonia, dissolved oxygen content, temperature and pH were monitored. After one month of feeding, growth increments of postlarvae fed with 30% protein diet were significantly different from those given 0; 40 and 50%. Survival rate of the group fed 40% protein was higher (81.7%) but was not significantly different from those fed 30% (68.0%). The highest feed efficiency was obtained from the 30% protein pellets (4.6). At the end of 16 weeks, growth increment of 40% protein level was significantly different from the 30% and 50% protein levels, but the survival rate was not significantly different from the 30% protein level. Feed conversion for the 40% protein diet was 2.8.

Incidence and low temperature survival of Salmonella in fishery products

Salmonella was isolated from 12% of PD shrimps, 10% of HL shrimps, 14% of PUD shrimps, 17% of lobsters, 14% of cuttle fish, 25% of cat fish and 20% of seer fish (all frozen) tested. One percent of the fish meal, 4% of dried non-penaeid prawn and 23% of sea beach sand showed incidence of the organism. Salmonella was also isolated from 2 and 4% of the swab samples of utensil surfaces and the floor surface of the processing hall respectively as well as from 1% of the process water tested. All the serotypes of Salmonella tested were resistant to freezing at -40°C, but during subsequent storage at -20°C, there was some difference between the serotypes with regard to their viability, S. paratyphi B being the most resistant which survived up to 9 months while S. saintpaul the least resistant having survival up to 5 months only.

Influences of dietary lipid and phosphorus levels on retention and excretion of phosphorus and nitrogen in fingerlings red sea bream, Pagrus major

A laboratory based 2x3 factorial experiment was conducted for 12 weeks to investigate the influences of dietary lipid and phosphorus (P) levels on retention and excretion of phosphorus and nitrogen (N) in fingerling red sea bream. Two levels of lipid (210 and 260 g/kg) and three levels of phosphorus (17, 14 and 12 g/kgˉ¹) in the dry diets were tested. Duplicate groups of 25 red sea bream (average weight 3.74±0.07 g) per 60L glass tank were fed experimental diets three times a day near to satiation level at 22 to 28°C water temperature. A reduction in dietary fish meal from 500 to 300 g/kg dry diet, corresponding to a supplementation in both dietary lipid and P resulted in significant increase in both P and N retention which resulted in the reduction of their excretion by red sea bream. The overall results of the present study demonstrated that both lipid and phosphorus supplementation are necessary for developing less-polluting feed which in turn, reduce fish meal level in the diet of fingerling red sea bream. Further studies in this regard with different size and age groups of red sea bream are warranted.