Polpa de jambolão (Eugenia jambolana Lam.) fresca e desidratada: características físico-químicas, bioativas e funcionais, efeitos biológicos em Caenorhabditis elegans e uso para produção de frozen yogurt caprino probiótico

This work evaluated the fresh, spray dried (with 10 % of Arabic Gum) and freeze dried jambolan pulp (Eugenia jambolana Lam.) in regard to physicochemical (pH, moisture, water activity, average particle diameter, solubility and color), bioactive [total phenolic content (TPC), monomeric anthocyanin, pronathocyanidin (PA), total elagic acid (TEA), myricetin and cyanidin] and in vitro functionality (antioxidant, antienzymatic and antimicrobial activities]. In addition, the in vivo functionality of jambolan pulp was investigated using the Caenorhabditis elegans model for insulin signaling, longevity and induced neurodegeneration (Alzheimer’s disease and Parkinson’s disease related symptoms). The dried jambolan pulp presented TPC retention (50% to 75%), PA (90% to 98%), TEA (31% to 83%), myricetin (40% to 84%), cyanidin (72% to 84%) and antioxidant activity (15%). The fresh jambolan pulp, the freeze dried pulp and the spray dried jambolan pulp presented high enzymatic inhibitory activity against pancreatic lipase (4,4 to 5,8 mg/mL), alpha-glycosidase (10,3 to 13,8 mg/mL) and alpha-amylase (8,9 to 11,2 mg/mL). They also were active inhibitors against the pathogen S. aureus. The dried jambolan experimental samples were able to increase the expression of several genes linked to the insulin signaling pathways (SIR-2.1, PPTR-1, DAF-16, SOD-3, e CTL) and increased the lifespan in C. elegans (18,07 % - 24,34 %), besides decreasing the amyloid AB1-42 aggregation induced paralysis and MPP+ (1-methyl-4-phenylpyridinium) induced neurodegeneration. Based on that, the jambolan pulp and the spray dried jambolan pulp were further selected for the production of caprine frozen yogurt with the addition of Bifidobacterium animalis subsp. lactis BI-07. The final product were evaluated in regard to their physicochemical (pH, acidity, total solids, protein, total reducing sugars, fat, ashes, overrun, melting test), bioactive (TPC and monomeric anthocyanin, antioxidant activity, probiotic viability and sensory analysis (sensory acceptance). The results showed that samples with probiotic had lowest pH and higher acidity, TPC, anthocyanin and antioxidant activity. It was also observed low overrun (14.2% to 22.6%). vi Samples with probiotic had lower flavor scores. Overall, this research presents the jambolan as a highly functional bioactive-rich fruit with the potential to modulate important biological pathways, extend lifespan and retard the development of neurodegenerative diseases. Jambolan is an underexploited exotic fruit with a high colorant potential and this thesis shows for the first time in the literature important technological, biological and scientific data about this fruit that could be used towards the development of health-oriented food products.

Capacidade de adesão e formação de biofilme de Candida ssp. isoladas da cavidade oral de pacientes transplantados renais na presença do extrato de Eugenia uniflora

Candidiasis is a major oral manifestation in kidney transplant patients. Candida spp. possess essential virulence factors which contribute for the infectious process, including the ability to adhere to epithelial cells and biofilm formation. The extract obtained from the leaves of Eugenia uniflora [acetone: water (7:3, v/v)] has demonstrated antifungal activity against Candida spp. This study evaluated the influence of the extract of E. uniflora in adhesion to human buccal epithelial cells (HBEC) and biofilm formation of 42 strains of Candida spp. isolated from the oral cavity of kidney transplant patients. Candida spp. strains belonging to a culture collection were reactivated and phenotypically re-identified by classical and molecular methods (genotyping ABC and RAPD), when necessary, to complete the identification to the species level. For the virulence tests evaluated in vitro, yeasts were grown in the presence and absence of 1000 g/mL of the extract. A ratio of 10: 1 (Candida spp. cells x HBECs) was incubated for 1 hour at 37 ° C, 200 rpm, fixed with 10% formalin and the number of Candida cells adhered to 150 HBEC determined by optical microscope. Biofilms were formed on polystyrene microplates in the presence or absence of the extract. The quantification was performed with crystal violet staining at 570 nm. All isolates were viable and exhibited phenotypic characteristics suggestive of each species identified. Two strains presumptively identified as Candida dubliniensis belonged to this species as determined with genotyping ABC, while strains identified as belonging to the Candida parapsilosis species complex were differentiated by RAPD genotyping. Candida albicans was found to be the most adherent species to the buccal epithelia, while C. tropicalis showed remarkable biofilm formation.We could detect that the extract of E. uniflora was able to reduce adhesion to HBEC for both Candida albicans and non-Candida albicans Candida species. On the other hand, only 16 Candida spp. strains (36 %) showed reduced biofilm formation. However, two highly biofilm producer strains of C. tropicalis had an expressive reduction in biofilm formation. This study reinforces the idea that besides growth inhibition, E. uniflora may interfere with the expression of some virulence factors of Candida spp., and may be possibly applied in the future as a novel antifungal agent.

Representações de ciência e tecnologia em Gustavo Barroso (1909-1935): nacionalismo autoritário, eugenia e antissemitismo

This study focuses on the representations of science and technology in the thinking of the Brazilian intellectual Gustavo Barroso in the period between 1909 and 1935. As one of the main leaders of the Brazilian Integralism, we will discuss,especially, his eugenic vision, prior to his participation in integralism and the influence of this science in building his authoritarian and anti-Semitic thinking. We seek to realize Gustavo Barroso dialogue, in a context of profound social and political changes, with the search for a nationalism "truly Brazilian." The methodology used was of a documentary and literature research , highlighting the analysis of Gustavo Barroso books, which were used as primary sources: Intelligencia das Coisas(1923), Aquém da Atlântida (1931), Brasil Colônia de Banqueiros (1934), O Integralismo de Norte a Sul (1934), O Quarto Império (1935) e A Palavra e o Pensamento Integralista (1935), and, also, some of his articles surveyed in the National History Museum collection. The survey results show that in this period Gustavo Barroso went on to develop in his writings an eugenic and authoritarian political vision, later, in his integralist phase, linked strongly to an anti-Semitic view.

Evaluación de la palatabilidad de Eugenia myrtifolia, justicia carnea y monstera deliciosa con potencial para la alimentación de marimonda (Ateles fusciceps robustus), en la fundación zoológico Santacruz, Cundinamarca

Tesis (Zootecnista). -- Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Zootecnia, 2013

Efeito de fungos micorrízicos arbusculares e da adubação no crescimento de mudas de Eugenia uniflora L., produzidas em diferentes substratos.

O objetivo deste estudo foi avaliar o efeito de fungos micorrízicos arbusculares (FMAs), da adubação e da composição do substrato no crescimento de mudas de Eugenia uniflora. As sementes foram germinadas em vermiculita média e repicadas para tubetes (100 cm3) contendo substratos à base de vermicomposto e casca de arroz carbonizada e, como controle, utilizou-se do substrato comercial à base de casca de pínus. Estes substratos foram testados com e sem inoculação micorrízica, adicionada ao substrato, como também se testaram a presença e a ausência de adubação de cobertura. Foram analisadas as propriedades físico-químicas dos substratos formulados. Avaliaram-se a altura, o diâmetro do colo, a agregação das raízes ao substrato, a biomassa seca aérea, a biomassa seca radicial e foram determinados a relação entre altura e diâmetro do colo e o índice de qualidade de Dickson. A inoculação com FMAs não influenciou no crescimento das mudas, enquanto a interação entre substratos e adubação foi significativa para a maioria das variáveis. A ausência de resposta aos FMAs foi, provavelmente, devido às altas concentrações de fósforo nestes substratos. Concluiu-se que o substrato à base de vermicomposto e casca de arroz carbonizada, na proporção de 20/80, pode ser utilizado na produção de mudas desta espécie.

María Eugenia Dengo Obregón: vislumbre del pensamiento filosófico y humanista de su obra

María Eugenia Dengo es una de las principales pensadoras y educadoras del siglo XX, su obra está sumergida en una serie de ideas filosóficas y humanistas que le permitieron vislumbrar algunas de las necesidades educativas costarricenses. Con el estudio y seguimiento de varios filósofos costarricenses, empezando con su padre Omar Dengo, su tesis basada en los poemas de Roberto Brenes Mesén y de la mano con Dra. Emma Gamboa, inició un largo camino en la investigación educativa con cimentos humanistas. Palabras claves: María Eugenia Dengo, educación, humanismo, pensadores/as costarricenses, ideas filosóficas costarricenses. Abstract María Eugenia Dengo is one of the most outstanding thinkers and teachers of the 20th. Century in Costa Rica. Her work, developed throughout her philosophical and humanistic thinking, fulfilled some of the most urgent educational needs of the country. Throughout the study of several Costa Rican thinkers, starting by his own father, Omar Dengo, followed by her thesis dissertation on Roberto Brenes Mesén poems and side by side with Dr. Emma Gamboa, Dengo set off the road in educational research and its humanistic foundations. Key words: Maria Eugenia Dengo, Education, Humanism, Costa Rican thinkers, Costa Rican philosophical tought

Reseña de “Las sociedades cacicales de Costa Rica (Siglo XVI)”, de Eugenia Ibarra Rojas

Introducción Los investigadores del Centro de Investigaciones Históricas de la Universidad  de Costa Rica ya han publicado cuatro títulos de  la Colección de Historia, entre los cuales se cuenta esta obra de Eugenia Ibarra. El trabajo dentro del grupo del Centro, que tiene diversas especialidades, ha contribuido a que “Las sociedades cacicales,” aunque es  un libro dedicado a ese tema en un siglo especifico, se pueda leer con la sensación de que ese trozo de la historia no esta aislado sino que es parte integra de la totalidad que es todo el acontecer de  la vida humana en el país desde sus inicios hasta el presente.

A chance for diversity? Australian online journalism

Australia’s mainstream media landscape has long been recognised as highly limited – media ownership in the country has traditionally been concentrated in the hands of a very few, and (except for Sydney and Melbourne) it is common for major Australian cities to be served by only one local newspaper, usually produced by Rupert Murdoch’s News Ltd. This can be seen also to affect the quality and diversity of Australian journalism; additionally, the global decline of newspaper publishers’ revenues and overall adverse economic conditions exert further pressure on journalistic operations in the country. At the same time, and possibly in response to the increasing stresses on industrial journalism in the country and the implications they have for the quality of journalistic products, a vibrant and dynamic ecosystem of Australian industrial and citizen journalism publications has emerged online. Existing media organisations have built strong news brands online, while citizen journalists and political bloggers have given voice to issues, concerns, and opinions hitherto underrepresented in Australian mainstream journalism; of particular interest, however, is the increasing level of engagement and interaction between the two. While such interaction has been characterised by deep animosity at times (especially also in the context of the Australian federal election in November 2007), Australia has also seen the emergence and establishment of a number of new, intermediary online publications which act as spaces for public debate and analysis – from the public intellectualism of Online Opinion through the muckraking of Crikey to the progressive politics of New Matilda. The rise of social media as spaces for the discussion of news and politics further changes the media environment, potentially leading both to renewed conflict between professional and citizen journalists and to a greater level of engagement between journalists and audiences. Overall, then, such online developments offer a chance for a greater diversity of opinion and representation in Australian journalism, but also remain under a cloud from uncertain long-term business models and funding arrangements. This chapter outlines current trends in Australian online journalism, and speculates about their effect on the Australian news media landscape.

Yellow Peril

This catalogue essay was written to accompany Eugenia Lim's 2015 exhibition at Metro Arts, Brisbane, 'Yellow Peril'. It discusses Lim's exploration of her identity as a Chinese-Australian, providing contextual information of Chinese immigration during Australia's gold rush years and the work of Hong Kong born American artist, Tseng Kwong Chi. It presents Yellow Peril as an exhibition that speaks critically about art and our cultural history amidst a fine layering of imagery, object and context, with a vitality that is derived from its rich and compelling base in real life moments and connections.

Impact of the invasive rust Puccinia psidii (myrtle rust) on native Myrtaceae in natural ecosystems in Australia

The invasive rust Puccinia psidii (myrtle rust) was detected in Australia in 2010 and is now established along the east coast from southern New South Wales to far north Queensland. Prior to reaching Australia, severe damage from P. psidii was mainly restricted to exotic eucalypt plantations in South America, guava plantations in Brazil, allspice plantations in Jamaica, and exotic Myrtaceous tree species in the USA; the only previous record of widespread damage in native environments is of endangered Eugenia koolauensis in Hawai’i. Using two rainforest tree species as indicators of the impact of P. psidii, we report for the first time severe damage to endemic Myrtaceae in native forests in Australia, after only 4 years’ exposure to P. psidii. A 3-year disease exclusion trial in a natural stand of Rhodamnia rubescens unequivocally showed that repeated, severe infection leads to gradual crown loss and ultimately tree mortality; trees were killed in less than 4 years. Significant (p < 0.001) correlations were found between both incidence (r = 0.36) and severity (r = 0.38) of P. psidii and subsequent crown loss (crown transparency). This provided supporting evidence to conclude a causal association between P. psidii and crown loss and tree mortality in our field assessments of R. rubescens and Rhodomyrtus psidioides across their native range. Assessments revealed high levels of damage by P. psidii to immature leaves, shoots and tree crowns—averaging 76 % (R. rubescens) and 95 % (R. psidioides) crown transparency—as well as tree mortality. For R. psidioides, we saw exceptionally high levels of tree mortality, with over half the trees surveyed dead and 40 % of stands with greater than 50 % tree mortality, including two stands where all trees were dead. Tree mortality was less prevalent for R. rubescens, with only 12 % of trees surveyed dead and two sites with greater than 50 % mortality. Any alternative causal agents for this tree mortality have been discounted. The ecological implications of this are unclear, but our work clearly illustrates the potential for P. psidii to negatively affect Australia’s biodiversity.

Efecto de distancias de siembra y métodos mecánicos y químico de control de malezas sobre la cenosis y el crecimiento y rendimiento del frijol común (Phaseolus vulgaris L.), postrera, 1994

El ensayo se estableció durante la postrera de 1994 (septiembre a diciembre) en la estación experimental La Compañía, ubicado en el municipio de San Marcos, Carazo, con el propósito de conocer el efecto de distancias de siembra y control químico y mecánico de malezas sobre el comportamiento de las malezas y el crecimiento y rendimiento de frijol común. Se utilizó un diseño de Bloque Completo al Azar en arreglo bifactorial, siendo el factor A: distancias de siembra (20, 40 y 60 cm. entre los surcos), y el factor B: control de malezas (todo el tiempo enmalezado, control mecánico durante el período crítico y control químico). Los datos obtenidos fueron sometidos al análisis de varianza y separación de medias de rangos múltiples de Duncan, al 5 %. Los resultados pueden ser resumidos de la siguiente manera: entre las malezas que más se encontraron en el campo están Melampodium divaricatum, Richardia scabra, Cyperus rotundus. Sorgum halepense. Bidens pilosa, entre otras. El tratamiento que presentó mayor número de individuos de malezas fue todo el tiempo enmalezado y el de menor enmalezamiento el control químico. De las distancias de siembra utilizadas, la que presentó mejor competencia con las malezas y donde se obtuvieron los mejores rendimientos fue en la distancia de 0.40 m. El uso de herbicidas post-emergente permitió un control casi total sobre las malezas y un elevado rendimiento que superó a los otros tipos de control. Después de analizar los resultados se puede indicar que es suficiente una limpia mecánica a los 22 días después de la siembra para mantener niveles bajos de malezas y un rendimiento no muy por debajo de aquellos donde se realiza más de una limpia. En las parcelas donde no se controló la maleza, se obtuvo el más bajo rendimiento y las malezas se mantuvieron siempre en niveles altos por lo tanto existió mayor competencia con el cultivo. La distancia de siembra de mejor rentabilidad fue la de 0.40 m. En los tipos de control el de mejor rentabilidad fue el control químico, el cual presentó un rendimiento alto, lo cual le permite rentabilidad de más del cien porciento.

Sapientia, 1997, Vol. LII, nº 201 (número completo)

Contenido: El aristotelismo y el tomismo frente al egoísmo psicológico / María L. Lukac de Stier – El deseo natural de ver a Dios en la Summa contra Gentiles / Guillermo Jorge Cambiasso – La creación como encuentro del ser y de la nada en la teología del maestro Eckhart de Hochheim O.P. (1260-1327) / Brian J. Farrelly – Malebranche y el libertinage érudit / Francisco Leocata – The problem of person and Jean Mouroux / John M. McDermott ; Glenn J. Comandini – Los fundamentos de la Bioética de H. Tristram Engelhardt / Jorge Martínez Barrera – Cuando las contradictorias son verdaderas / Marisa Villalba de Tablón – Examen del escepticismo moral y del relativismo moral / Camilo Tale – Saint Thomas et le mystère de la création : une réponse aux interrogations de l’homme d’aujourd’hui / Marie-Dominique Philippe – La finalidad de la naturaleza humana : alcance y actualidad de la cuestión / Beatriz Eugenia Reyes Oribe – La providencia y el gobierno del mundo / Alberto Caturelli – Saint Thomas et le problème de la possibilité d’un univers créé éternel / Alain-Marie de Lassus – La causalidad material de los elementos en la generación de los cuerpos mixtos / Mario Enrique Sacchi -- Bibliografía

Saber y poder, prodigios ambiguos e interpretación medieval en el Libro de Alexandre y el Poema de Fernán Gonçález

Resumen: Tanto en el Libro de Alexandre como en el Poema de Fernán Gonçález se presentan prodigios de signifi cación ambigua: el eclipse en el LA, el caballero tragado por la tierra y la serpiente voladora en el PFG. Los episodios en que aparecen insertos estos elementos extraordinarios o sobrenaturales ofrecen una serie de similitudes: el temor de los ejércitos y la habilidad retórica que se despliega para reinterpretar el signo, volverlo a favor del héroe y enardecer así a sus hombres. El objetivo de este trabajo es examinar estos episodios y su signifi cación en el marco de cada uno de los poemas, centrándonos en los problemas de la interpretación y la asignación de sentido, y la relación entre saber y poder que se postula.

Evolución y perspectivas de la educación en Argentina, su cobertura y su calidad

Resumen: El objetivo central de este trabajo es analizar la situación educativa de la población argentina en relación con otros países y su evolución en los últimos años. Se observa que la "cantidad de educación" sigue siendo muy importante, aunque también se observa recientemente una caída en la matrícula del nivel medio y una reducción en la matrícula de las escuelas públicas a nivel primario. Asimismo, su distribución continúa siendo muy desigual –aunque es una de las menos desiguales en América Latina– y su calidad, en base a los resultados de pruebas internacionales, es pobre en comparación con otros países.