Allosteric Modulation of the HIV-1 gp120-gp41 Association Site by Adjacent gp120 Variable Region 1 (V1) N-Glycans Linked to Neutralization Sensitivity

The HIV-1 gp120-gp41 complex, which mediates viral fusion and cellular entry, undergoes rapid evolution within its external glycan shield to enable escape from neutralizing antibody (NAb). Understanding how conserved protein determinants retain functionality in the context of such evolution is important for their evaluation and exploitation as potential drug and/ or vaccine targets. In this study, we examined how the conserved gp120-gp41 association site, formed by the N- and Cterminal segments of gp120 and the disulfide-bonded region (DSR) of gp41, adapts to glycan changes that are linked to neutralization sensitivity. To this end, a DSR mutant virus (K601D) with defective gp120-association was sequentially passaged in peripheral blood mononuclear cells to select suppressor mutations. We reasoned that the locations of suppressors point to structural elements that are functionally linked to the gp120-gp41 association site. In culture 1, gp120 association and viral replication was restored by loss of the conserved glycan at Asn136 in V1 (T138N mutation) in
conjunction with the L494I substitution in C5 within the association site. In culture 2, replication was restored with deletion of the N139INN sequence, which ablates the overlapping Asn141-Asn142-Ser-Ser potential N-linked glycosylation sequons in
V1, in conjunction with D601N in the DSR. The 136 and 142 glycan mutations appeared to exert their suppressive effects by altering the dependence of gp120-gp41 interactions on the DSR residues, Leu593, Trp596 and Lys601. The 136 and/or 142
glycan mutations increased the sensitivity of HIV-1 pseudovirions to the glycan-dependent NAbs 2G12 and PG16, and also pooled IgG obtained from HIV-1-infected individuals. Thus adjacent V1 glycans allosterically modulate the distal gp120-
gp41 association site. We propose that this represents a mechanism for functional adaptation of the gp120-gp41 association site to an evolving glycan shield in a setting of NAb selection.

Developing Abu Dhabi's solid waste sustainability index (ADSWMI)

Fast urbanization and population and economic growth led to increased solid waste generation in Abu Dhabi in the last decades. Abu Dhabi generates 5.8 kg of municipal waste per day per person. This is well above the world average of 1.2 kg per day per person. Treatment and destination of the municipal solid waste is also problematic. Only 3.5% of the total municipal solid waste generation is recycled, and the remaining waste is disposed in landfills which are technically not adequate. In this context, sustainability indicators can play an important role in supporting decision makers in planning and managing the solid waste system. In this study, the waste management system in Abu Dhabi Emirate was analyzed through the implementation of a set of proposed sustainability indicators. The DSR Driving force-State-Response approach was used as the methodology to develop a framework for the context of Abu Dhabi. Twenty indicators, based on literature review and benchmarking, were divided into five categories: quantity & composition, environmental controls & resource management, construction & demolition waste, financial sustainability, and governances & policies. These indictors can be a baseline to assist decision makers to develop an integrated waste management system able to meet the high international standards and target in the field.

The influence of cover on nesting Red-capped Plovers: a trade-off between thermoregulation and predation risk?

 Some ground-nesting birds adopt a mixed strategy of nesting in the open, or under cover (e.g. vegetation). This may represent a trade-off between thermally favourable nest sites (covered) and those that enable the early detection and avoidance of predators (open). This study examined whether such a trade-off exists for Redcapped Plover Charadrius ruficapillus, whose eggs are preyed upon principally by Little Raven Corvus mellori. For real and artificial nests, nest temperatures under cover (real, 25.9 ± 0.1°C; false, 16.2 ± 0.5°C) were cooler than those in the open (real, 26.8 ± 0.1°C; false, 17.4 ± 0.9°C). Covered nests had more visual obstructions than open nests (covered, 65.5% ± 11.4%; open, 7.4% ± 2.8%) and a standardised measure of incubator escape distance, initiated by experimental human approaches, indicated incubators fled open nests at longer distances than for covered nests. Nests under cover showed a slightly (non-significant) higher probability of surviving one day (Daily Survival Rate [DSR] = 0.978) than those in the open (DSR = 0.950). For false nests containing model eggs, covered nests exhibited better survival to 10 days compared with open nests (20.4% vs. 4.7%). Thus, covered nests are associated with enhanced thermal environments and egg survival, but predators can approach the incubator more closely. Overall, the proposed trade-off between thermal and predation risks associated with nest sites appears to exist and explains the ongoing occurrence of nests in open and covered locations.

Atributos físicos nos tempos de adoção de manejos em Latossolo cultivado com soja

A condução das operações de preparo de forma inadequada ocasiona sérios problemas de conservação do solo, destacando-se a compactação, que acarreta a redução do espaço poroso, principalmente dos macroporos, e altera os atributos físico-hídricos. Este trabalho teve como objetivo verificar a influência dos diferentes sistemas e tempos de adoção de manejos em Latossolo Vermelho de Jaboticabal, Estado de São Paulo, por meio da densidade máxima, e correlacioná-la com a produtividade da soja, a densidade relativa e a umidade crítica de compactação. O delineamento experimental foi o inteiramente casualizado com parcelas subdivididas (cinco sistemas de uso e três camadas), com quatro repetições. Os cinco sistemas de uso foram: plantio direto por cino anos (SPD5), plantio direto por sete anos (SPD7), plantio direto por nove anos (SPD9), preparo convencional (SPC) e uma área adjacente de mata nativa (MN). As camadas do solo avaliadas foram as de 0-0,10, 0,10-0,20 e 0,20-0,30 m, nas quais foram determinados a densidade máxima do solo (Ds máx), a umidade crítica de compactação (Ugc), a densidade relativa do solo (Dsr), a composição granulométrica, a porosidade e o teor de matéria orgânica do solo. Os resultados mostraram que o comportamento das curvas de compactação do solo foi o mesmo em todas as camadas dos diferentes manejos e que os teores de matéria orgânica não justificaram as pequenas alterações da Ds máx. Para o Latossolo Vermelho, as operações mecanizadas nos sistemas de manejo podem ser executadas na faixa de 0,13 a 0,19 kg kg-1 de umidade sem causar degradação física. Verificou-se que a Dsr ótima e a umidade crítica de compactação foram de 0,86 e 0,15 kg kg-1, respectivamente, embora os diferentes sistemas e tempos de adoção de manejo tenham apresentado comportamento semelhante quanto à produtividade da soja.

Densidade relativa ótima de Latossolos Vermelhos para a produtividade de soja

A compactação do solo tem causado decréscimos na produtividade de soja, e os valores de densidade do solo a partir dos quais a produtividade decresce são pouco conhecidos. Assim, este trabalho objetivou avaliar a produtividade de soja (cv. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) 48) em relação à densidade relativa (Dsr) de Latossolo Vermelho textura média (LVd) e argilosa (LVef). em 2001/2002, foi realizado um experimento em casa de vegetação, com amostras coletadas nos dois solos na camada de 0,0-0,2 m, peneirados a 4,0 mm, adubados e compactados em camadas de 3,0 cm, em vasos de 20 cm de altura e 25 cm de diâmetro. Foram estabelecidos quatro níveis de compactação e dois de água no solo (tensão de 0,01 e 0,05 MPa), com três repetições. A soja foi semeada em novembro (duas plantas por vaso) e irrigada duas vezes ao dia. No segundo experimento, no campo (2002/2003), utilizou-se apenas o LVd que foi compactado por meio de passadas de trator com quatro pneus de mesma largura, estabelecendo-se cinco níveis de compactação, com quatro repetições. A soja foi semeada, em dezembro de 2002, no espaçamento de 0,45 m entre linhas e 20 plantas por metro. Foram determinadas a densidade do solo (Ds) e a Ds máxima pelo teste de Proctor, sendo a Dsr obtida por divisão da Ds pela Ds máxima. Na colheita, foi avaliada a produtividade de grãos. A Dsr ótima para a produtividade de soja, em casa de vegetação, foi superior no Latossolo Vermelho eutroférrico argiloso (0,84), comparada à do Latossolo Vermelho caulinítico de textura média (0,75), na tensão de água no solo de 0,01 MPa. No campo, a Dsr ótima para soja foi de 0,80.

Desenvolvimento e implantação de um sistema de gestão de pedidos de informação endereçados à Universidade Federal do Rio Grande do Norte a partir do serviço de informações ao cidadão (E-SIC)

Among the numerous policy changes that the world has experienced in recent years, occupies a prominent place in the quest for greater transparency of public agencies. Transparency has been an important tool in the accountability of the State to promote greater participation of the society by providing information that was previously restricted knowledge of public agencies. Brazil, following this trend, promulgated in May 2012 the Access to Information Act that seeks to disclose the actions of the State at all levels, in all public administration agencies. On the same day of the enactment of the law is provided society with a site that is empowering citizens to make their requests for information to government agencies. The Federal University of Rio Grande do Norte, which at that time had no a tool to assist them in managing this demand. This project has the objective to describe, build and implement a solution to solve this problem using Design Science Research as methodology. As result, the solution built in this research became a new module of the institution s ERP became it capable to control the entire process, and will be helpful to others partners which use our system ERP

Effect of diabetes mellitus and insulin therapy on bone density around osseointegrated dental implants: a digital subtraction radiography study in rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of simulated external root resorptions with digital radiography and digital subtraction radiography

Introduction: Root resorption can cause damage in orthodontic patients. Digital subtraction radiography (DSR) is a useful resource for the detection of mineral losses. The purpose of this study was to compare the efficacy of digital radiography (DR) and DSR in detecting simulated external root resorption. Examiner agreement between the 2 techniques was also evaluated. Methods: Root resorptions of various sizes were simulated on the apical and lingual aspects of 49 teeth from 9 dry human mandibles. The teeth were radiographed in standardized conditions. The radiographs were registered with Regeemy Image Registration and Mosaicking (version 0.2.43-RCB, DPI-INPE, Sao Jose dos Campos, São Paulo, Brazil) and subtracted with Image Tool (University of Texas Health Science Center at San Antonio). The subtracted images and the digital radiographs were evaluated by 3 oral radiologists. Results: No statistically significant differences were found for the methods in the detection of apical root resorptions, independently from lesion size, and of lingual resorptions of 1.2 mm or greater. DSR was significantly better than DR for detection of lingual resorptions up to 1 mm. Resorptions less than 0.5 mm were not precisely detected by either method. DSR provided better intraexaminer and interexaminer agreement than did DR. Conclusions: Both methods are precise for detection of apical root resorptions as small as 0.5 mm and lingual resorptions of 1 mm or more. However, DSR frequently performed better than did DR. (Am J Orthod Dentofacial Orthop 2011;139:324-33)

Determinação da velocidade de um objeto, a partir de uma seqüência de imagens, aplicando as equações de colinearidade modificadas

Pós-graduação em Ciências Cartográficas - FCT

Cuestiones principales sobre el desarrollo social rural en América Latina: versión preliminar

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Grupos indígenas y el desarrollo social: versión preliminar

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Rol de la agroindustrialización latinoamericana y su impacto sobre el desarrollo agropecuario: versión preliminar

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Análisis de las interrelaciones entre la agricultura y la industria: versión preliminar

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Los grupos indigenas y el desarrollo social

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Tendencias demográficas

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