Synonymy of Perkinsus olseni Lester & Davis, 1981 and Perkinsus atlanticus Azevedo, 1989 and an update on the phylogenetic position of the genus Perkinsus

Sequences of the rRNA nontranscribed spacer (NTS) were determined for six isolates of Perkinsus olseni, seven isolates of Perkinsus sp. from Anadara trapezia and one isolate of Perkinsus sp. from Austrovenus stutchburyi. These sequences were compared with previously published NTS sequences for R atlanticus, P. marinus and P. andrewsi. Consensus sequences for Perkinsus olseni, the Perkinsus isolates and P. atlanticus were approximately 98-99% similar to each other but only 65-79% similar to P. marinus and P. andrewsi sequences. Some individual P. olseni sequences were less similar to each other (97.4%) than they were to P. atlanticus sequences (97.8-98.2%), therefore NTS provides further evidence that P. atlanticus, P. olseni, Perkinsus sp. from Anadara trapezia and Perkinsus sp. from Austrovenus stutchburyi are conspecific. We propose that P. atlanticus be synonymised with P. olseni Lester & Davis, 1981 which has taxonomic priority, and that Perkinsus sp. from Anadara trapezia and Perkinsus sp. from Austrovenus stutchburyi belong to R olseni sensu lato as well. A phylogenetic analysis of SSU rDNA, incorporating recently published Perkinsus sequences, supports the placement of the Perkinsus species with Parvilucifera infectans within the Dinoflagellata.

Human risk assessment of benzene after a gasoline station fuel leak

OBJECTIVE: To assess the health risk of exposure to benzene for a community affected by a fuel leak. METHODS: Data regarding the fuel leak accident with, which occurred in the Brasilia, Federal District, were obtained from the Fuel Distributor reports provided to the environmental authority. Information about the affected population (22 individuals) was obtained from focal groups of eight individuals. Length of exposure and water benzene concentration were estimated through a groundwater flow model associated with a benzene propagation model. The risk assessment was conducted according to the Agency for Toxic Substances and Disease Registry methodology. RESULTS: A high risk perception related to the health consequences of the accident was evident in the affected community (22 individuals), probably due to the lack of assistance and a poor risk communication from government authorities and the polluting agent. The community had been exposed to unsafe levels of benzene (> 5 µg/L) since December 2001, five months before they reported the leak. The mean benzene level in drinking water (72.2 µg/L) was higher than that obtained by the Fuel Distributer using the Risk Based Corrective Action methodology (17.2 µg/L).The estimated benzene intake from the consumption of water and food reached a maximum of 0.0091 µg/kg bw/day (5 x 10-7 cancer risk per 106 individuals). The level of benzene in water vapor while showering reached 7.5 µg/m3 for children (1 per 104 cancer risk). Total cancer risk ranged from 110 to 200 per 106 individuals. CONCLUSIONS: The population affected by the fuel leak was exposed to benzene levels that might have represented a health risk. Local government authorities need to develop better strategies to respond rapidly to these types of accidents to protect the health of the affected population and the environment.

CDC PC station guide: tutorial

This is a walkthrough guide to install a fresh CDC PC Station on the Flexible Manufacturing Field Trial.

Final report detailed description - NET Station: shaping radio for web environment

In more scientific terms, NET Station contributed to: - discuss in theoretical terms the role radio still play in contemporary societies; - examine how audiences are using sound resources on the Web; - draw up a new theoretical framework for the study of the reconfiguration of radio language on the Internet;acknowledge that people are not abandoning radio as a medium; the survey applied by the team demonstrated that there is a complementariness between traditional radio and radio on the Internet; - understand that people expect more interactivity, more opportunities to participate in content production and more diversity of contents. These results were shared with editors responsible for Portuguese radio and might influence the offer made available by these radio corporations from now on; - demonstrate that the Internet is underexplored in terms of sound and acoustic communication; - promote the production of new sound narratives to be available on the Internet;

The shoulder architecture of bears and other carnivores [by] D. Dwight Davis.

v.31:no.34(1949)

Contributions from the Bermuda Biological Station for Research.

4, no.52-76

Efeitos de fitoreguladores no desenvolvimento da soja (Glycine max cv. Davis)

Verificou-se, em condições de casa de vegetação, o efeito de fitoreguladores no desenvolvimento da soja (Glycine max cv. Davis). Aplicou-se por pulverização, 21 dias após a semeadura, cloreto (2-cloroetil) trimetilamônio (CCC) 2000 ppm ácido succínico-2,2-dimetilhidrazida (SADH) 4000 ppm, ácido giberélico (GA) 100 ppm, ácido indolilacético (IAA) 100 ppm, além do controle. Realizaram-se determinações semanais da altura da planta, número total de folhas, número de folhas na haste principal, número de folhas nas hastes secundárias e diâmetro do caule. Utilizou-se delineamento inteiramente casualizado com 6 repetições e procedeu-se a comparação de médias pelo teste Tukey (5%). Plantas tratadas com GA mostraram

Desenvolvimento dos meritalos de soja (Glycine max cv. Davis) sob efeito de fitoreguladores

Determinou-se em condições de casa de vegetação o efeito de fitoreguladores no desenvolvimento dos meritalos da soja 'Davis'. Aplicou-se através de pulverização, 21 dias após a semeadura, cloreto (2-cloroetil) trimetilamônio (CCC) 2000 ppm, ácido succínico -2,2 - dimetilhidrazida (SADH) 4000 ppm, ácido geberélico (GA) 100 ppm, ácido indolilacético (IAA) 100 ppm, além do controle. Efetuaram-se determinações semanais do número de meritalos e do comprimento do segundo e quarto merítalo das plantas de soja. Utilizous-e delineamento inteiramente casualizado com 6 repetições e procedeu-se à comparação de médias pelo teste Tukey (5%). Tratamento com GA aumentou o numero de meritalos da soja 'Davis'. Não se notaram diferenças significativas no comprimento do segundo meritalo das plantas tratadas com fitoreguladores. SADH reduziu o comprimento do quarto meritalo das plantas de soja.

Efeitos de fitoreguladores na florescência e produtividade da soja(Glycine max cv. Davis)

Verificou-se em condições de casa de vegetação o efeito de fitoreguladores na florescência e produtividade da soja 'Davis'. Quando as plantas apresentavam 4 folhas definitivas, aplicou-se através de pulverização, cloreto (2-cloroetil) trimetilamonio (CCC) 2.000 ppm, ácido succínico-2,2-dimetilhidrazida (SADH) 4.000 ppm, ácido giberélico (GA) 100 ppm e ácido indolilacético (IAA) 100 ppm, além do controle. Efetuaram-se contagens diárias do número de flores e o número de vagens foi determinado semanalmente. Na colheita estabeleceram-se os parâmetros de produtividade. O delineamento experimental foi inteiramente casualizado com 6 repetições, procedendo-se a comparação de médias pelo teste Tukey ao nível de 5% de probabilidade. Observou-se que o SADH retardou o início da florescência, reduziu o período de florescimento e diminuiu o número de vagens produzidas. Aplicação de GA retardou a ocorrência da maxima florescência e aumentou o peso da matéria seca do caule da soja 'Davis'.