Avaliação do efeito do composto tipo heparina isolado do caranguejo Chaceon fenneri na hemostasia e na morte celular

Heparin is a pharmaceutical animal widely used in medicine due to its potent anticoagulant effect. Furthermore, it has the ability to inhibit the proliferation, invasion and adhesion of cancer cells to vascular endothelium. However, its clinical applicability can be compromised by side effects such as bleeding. Thus, the search for natural compounds with low bleeding risk and possible therapeutic applicability has been targeted by several research groups. From this perspective, this study aims to evaluate the hemorrhagic and anticoagulant activities and citotoxic effect for different tumor cell lines (HeLa, B16-F10, HepG2, HS-5,) and fibroblast cells (3T3) of the Heparin-like from the crab Chaceon fenneri (HEP-like). The HEP-like was purified after proteolysis, ion-exchange chromatography, fractionation with acetone and characterized by electrophoresis (agarose gel) and enzymatic degradation. Hep-like showed eletroforetic behavior similar to mammalian heparin, and high trisulfated /Nacetylated disaccharides ratio. In addition, HEP-like presented low in vitro anticoagulant activity using aPTT and a minor hemorrhagic effect when compared to mammalian heparin. Furthermore, the HEP-like showed significant cytotoxic effect (p<0.001) on HeLa, HepG2 and B16-F10 tumor cells with IC50 values of 1000 ug/mL, after incubation for 72 hours. To assess the influence of heparin-like on the cell cycle in HeLa cells, analysis was performed by flow cytometry. The results of this analysis showed that HEP-like influence on the cell cycle increasing S phase and decreasing phase G2. Thus, these properties of HEP-like make these compounds potential therapeutic agents

Avaliação do potencial anti-inflamatório de composto tipo heparina (cCTH) extraído do caranguejo Goniopsis cruentata em modelo experimental de peritonite

Heparin, a sulfated polysaccharide, was the first compound used as an anticoagulant and antithrombotic agent. Due to their structural characteristics, also has great potential anti-inflammatory, though such use is limited in inflammation because of their marked effects on coagulation. The occurrence of heparin-like compounds that exhibit anticoagulant activity decreased in aquatic invertebrates, such as crab Goniopsis cruentata, sparked interest for the study of such compounds as anti-inflammatory drugs. Therefore, the objective of this study was to evaluate the potential modulator of heparin-like compound extracted from Goniopsis cruentata in inflammatory events, coagulation, and to evaluate some aspects of its structure. The heparin-type compound had a high degree of N-sulphation in its structure, being able to reduce leukocyte migration into the peritoneal cavity at lower doses compared to heparin and diclofenac sodium (anti-inflammatory commercial). Furthermore, it was also able to inhibit the production of nitric oxide and tumor necrosis factor alpha by activated macrophages, inhibited the activation of the enzyme neutrophil elastase in low concentrations and showed a lower anticoagulant effect in high doses as compared to porcine mucosal heparin. Because of these observations, the compound extracted from crab Goniopsis cruentata can be used as a structural model for future anti-inflammatory agents

Adsorção de Pb(II) utilizando adsorvente composto por montmorilonita e dolomita

The destructive impact of improper disposal of heavy metals in the environment increases as a direct result of population explosion, urbanization and industrial expansion and technological developments. Argil are potential materials for adsorption of inorganic and the pelletization of it is required for use in adsorptive columns of fixed bed. The low cost and the possibility of regeneration makes these materials attractive for use in the purification process, capable of removing inorganic compounds in contaminated aquatic environments. In this work was made pellets of a mixture of dolomite and montmorillonite by wet agglomeration, in different percentages. The removal of Pb (II) was investigated through experimental studies, and was modeled by kinetic models and isotherms of adsorption. The materials were characterized using the techniques of XRD, TG / DTA, FT-IR, and surface area by BET method. The results showed the adsorption efficiency of the contaminant by the composite material studied in synthetic solution. The study found that the adsorption follows the Langmuir model, and the kinetics of adsorption follows the model of pseudosecond order

Do lixo à solidariedade : avanços e perspectivas na busca de uma nova realidade

The dissertating study about the solidarity economy has the objective to analyze the four unions responsible for the selective municipal garbage collection in Natal. It aims at verifying the consolidation of these unions as solidarity economic undertakings, revealing which progresses they have made, as well as the social and economic insertion of the garbage collectors and their process of conquering citizenship. The referred four unions had been founded and are constituted, in their majority, by collectors coming from the Cidade Nova lixão (big garbage). As it was closed in August 2004, they decided to make a union in order to collecting garbage. As what concerns the methodic and theoretic proceedings, our research has been developed with a critical perspective and a qualitative approach without discarding and quantitative one. The central analytical categories of this paper are: association, work, social exclusion and citizenship. Our research has had three articulated axis which aim was to apprehend the subject, disclosing it. The exposition of the investigative results is subdivided in four chapters. The first one approaches the main aspects of the crisis of the capital and its reflexes in the world of work. Here we deal with the question the structural unemployment coming as a result of the present economic model, the mains changes verified in the Brazilian work market, as well as levels of unemployment affecting the work market in Natal s metropolitan region. The second chapter treats of the origin, concept and revival in Brazil concerning the tradition of thought and cooperative economic organization, which has recovered the central elements of the associative thought and is nowadays studied in Latin America under the name of solidarity economy. The third chapter deals with embodiment of the collectors unions, its history, appearing and development of each union. The fourth chapter presents the relative dimensions of the analysis categories supported in the reports of institutional actors as well as the perception collectors have about the recyclable stuffs, the way they face the daily life and so on, what brings about the contradictions present in their reality. The final comments sum up the main trends and particularities of the unions researched under the light of the solidarity economy and disclose the real perspectives of social and economic insertion of these collectors and the process they follow to conquest social recognition

Avaliação dos parâmetros genéticos em progênies de meio-irmãos de uma sub-população de milho composto Flint para resistência à lagarta-da-espiga Helicoverpa zea (Bod.)

Foram estudadas 100 progênies de meio-irmãos de uma sub-população de milho (Zea mays L.) Composto Flint com o objetivo de avaliar a resistência de genótipos à lagarta-da-espiga Helicoverpa zea (Bod.). Foram obtidos os valores de danos médios da lagarta-da-espiga de 1,14 cm de comprimento na espiga determinado pela escala de Widstrom e coeficiente de variação experimental (CVE) de 23,4%. Dos parâmetros genéticos avaliados, a estimativa de herdabilidade (h²) foi de 6%, variância genética (VG) de 0,0015 cm² e variância fenotípica (VF) de 0,025 cm² para danos de H. zea. No entanto, o comprimento da ponta da bráctea e compactação da bráctea alcançaram resultados de herdabilidade de 75% e 72% respectivamente. Essa sub-população de milho apresenta variabilidade genética suficiente para utilização em programas de melhoramento, sendo que a resistência à lagarta-da-espiga pode ser obtida através da melhoria dos caracteres morfológicos diretamente relacionados à praga, como a compactação e comprimento da bráctea.

Formação de mudas de eucalipto com utilização de lixo orgânico e níveis de irrigação calculados por dois métodos

O estudo das características e das possibilidades de utilização de Resíduo Sólido Urbano (R.S.U.) e mais especificamente de Resíduo Sólido Orgânico Urbano (R.S.O.U.), no Brasil e no Mundo, ainda não produziu informações que possibilitem conhecimentos suficientes para tomadas de decisões que estão sendo exigidas, em razão do crescente volume de resíduos produzidos e os impactos ao meio ambiente. Este trabalho teve o objetivo de estudar o efeito da irrigação associada ao uso de R.S.O.U. para formação de mudas de eucalipto. A pesquisa foi desenvolvida na UNESP, Câmpus de Jaboticabal - SP, sendo adotado como delineamento estatístico o esquema fatorial inteiramente casualizado. Foram utilizados 11 substratos: dois tipos de solos (um Latossolo Vermelho eutrófico e um Latossolo Vermelho distrófico), com e sem adubação química, seis misturas de solo e R.S.O.U., e um utilizando somente R.S.O.U. Sementes de eucalipto var. Citriodora Hooker foram semeadas diretamente em topetes e irrigadas com base em dois métodos de quantificação da água evapotranspirada (tanque Classe A e determinação da massa), em quatro níveis (50; 70; 100 e 130% da evapotranspiração). Os dados coletados e analisados estatisticamente demonstraram que doses acima de 20% de R.S.O.U. na mistura, em solos destinados à produção de mudas de eucalipto, causam efeitos negativos na sobrevivência das plantas.

Extratores para avaliação da disponibilidade de metais pesados em solos adubados com vermicomposto de lixo urbano

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Emergência e crescimento inicial de plântulas de pimenta ornamental e celosia em substrato à base de composto de lodo de curtume

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Alterações em propriedades do solo adubado com composto orgânico e efeito na qualidade das sementes de alface

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Alterações em propriedades de solo adubado com doses de composto orgânico sob cultivo de bananeira

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação nutricional em folhas de bananeira 'Prata-anã' adubadas com composto orgânico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Composto orgânico na produção e qualidade de sementes de brócolis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Composto orgânico no potencial fisiológico de sementes de brócolis após o armazenamento

The study was carried out with the objective to evaluate the broccoli seeds (cultivar Ramoso Santana) germination and vigor from plants cultivated with different organic compost levels and after storage in dry chamber. At seed production stage, the experiment was conducted in randomized complete blocks design, with five treatments (0; 30; 60; 90 and 120 t ha(-1) of Biomix (R) organic compost) and four replications. Soon after harvesting and after 12 and 24 months of conservation in dry chamber room (20 degrees C and 40% relative humidity) it was evaluated germination, following seed analisys rules, and vigor (first count of germination and germination speed index - IVG). There were no differences in germination and vigor in seeds as soon as they were harvested and after 12 month of storage. After 24 months of storage, the lesser the organic compost rates, the lesser seed germination and IVG, with respective values, without compost, of 69% and 12,3. For another hand, the highest values for germination and IVG, 82% and 16,1, respectively, were obtained with the highest rate of organic compost (120 t ha(-1)).

Estudo térmico e de materiais na construção de casas populares com blocos confeccionados a partir de um composto a base de cimento, gesso, eps e raspa de pneu

We built an experimental house on an UFRN´s land using blocks made by a composite consisting of cement, plaster, EPS, crushed rubber and sand. Several blocks were made from various compositions and we made preliminary tests of mechanical and thermal resistance, choosing the most appropriate proportion. PET bottles were used inside the block to provide thermal resistance. In this work, a second function was given to the bottles: to serve as a docking between the blocks, because the ends of the cylinders came out of each block on top as well as at the bottom, with the bottom cut, allowing to fit of the extremities of the upper cylinder of a block in the lower holes of the other one, which were formed by the cutting already mentioned. Minimum compression tests were performed according to ABNT standards for walls closing blocks (fence). With that house built, we did studies of thermal performance in order to ascertain conditions of comfort, checking external and internal temperatures in the walls and in the ambient, among other variables, such as wind speed and relative humidity. The resulting blocks provided adequate thermal insulation to the environment, where the walls presented differences up to 11.7 ºC between the outer and inner faces, getting the maximum temperature inside the house around 31 °C, within the so-called thermal comfort zone for warm climates. At the end of the experiments it was evident the effectiveness of that construction in order to provide thermal comfort in the internal environment of the house, as well as we could confirm the viability of building houses from recyclable materials, reducing the constructive costs, becoming a suitable alternative for low- incoming families. Moreover, besides the low cost, the proposal represents an alternative use of various recyclable materials, therefore considered an ecological solution

Crescimento e produção de bananeira prata-anã adubada com composto orgânico durante cinco safras

O presente trabalho objetivou avaliar a influência da adubação orgânica durante cinco safras, nas características de crescimento e produtividade de plantas de bananeira-'Prata-anã'. O experimento foi instalado no município de Botucatu-SP, em novembro de 2002, com mudas convencionais, adotando-se o espaçamento de 2,5 x 2,5 m, sendo as plantas adubadas com composto orgânico produzido a partir de serragem de madeira e esterco bovino devidamente compostados, que constituíram os tratamentos: 0; 43; 86; 129 e 172 kg de composto por planta, sendo estas doses de composto calculadas de acordo com o teor de potássio presente no composto. No florescimento das plantas em cada ciclo (2003, 2004, 2005, 2006 e 2007), foram medidas a circunferência do pseudocaule, a altura de inserção da inflorescência e determinou-se o número de folhas úteis por planta. Na colheita, foram determinados a massa do cacho, o número de frutos por cacho, o número de pencas por cacho, a massa da 2ª penca, o número de frutos na 2ª penca, o comprimento e o diâmetro de frutos da 2ª penca. As doses de composto orgânico não causaram alterações nas características de crescimento das plantas; contudo, em função dos ciclos avaliados, foi possível observar queda no número de folhas a partir do segundo ciclo e alterações na altura de plantas e circunferência do pseudocaule. No presente trabalho, onde as doses de K2O oscilaram entre zero e 394 g por planta, os cachos com massa mais elevada foram obtidos com as duas maiores quantidades de compostos aplicadas, o que, economicamente, indica como a melhor dose a ser recomendada a de 129 kg de composto por planta, que fornece 290,5 g de K2O por planta.