Mechanism of Action and Relationship Between Structure and Biological Activity of Ctx-Ha: A New Ceratotoxin-like Peptide from Hypsiboas albopunctatus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Detection of P. aeruginosa harboring bla(CTX-M-2), bla(GES-1) and bla(GES-5), bla(IMP-1) and bla(SPM-1) causing infections in Brazilian tertiary-care hospital

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Dynamics and Conformational Studies of TOAC Spin Labeled Analogues of Ctx(Ile21)-Ha Peptide from Hypsiboas albopunctatus

Antimicrobial peptides (AMPs) isolated from several organisms have been receiving much attention due to some specific features that allow them to interact with, bind to, and disrupt cell membranes. The aim of this paper was to study the interactions between a membrane mimetic and the cationic AMP Ctx(Ile21)-Ha as well as analogues containing the paramagnetic amino acid 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) incorporated at residue positions n = 0, 2, and 13. Circular dichroism studies showed that the peptides, except for [TOAC13]Ctx(Ile21)-Ha, are unstructured in aqueous solution but acquire different amounts of α-helical secondary structure in the presence of trifluorethanol and lysophosphocholine micelles. Fluorescence experiments indicated that all peptides were able to interact with LPC micelles. In addition, Ctx(Ile21)-Ha and [TOAC13]Ctx(Ile21)-Ha peptides presented similar water accessibility for the Trp residue located near the N-terminal sequence. Electron spin resonance experiments showed two spectral components for [TOAC0]Ctx(Ile21)-Ha, which are most likely due to two membrane-bound peptide conformations. In contrast, TOAC2 and TOAC13 derivatives presented a single spectral component corresponding to a strong immobilization of the probe. Thus, our findings allowed the description of the peptide topology in the membrane mimetic, where the N-terminal region is in dynamic equilibrium between an ordered, membrane-bound conformation and a disordered, mobile conformation; position 2 is most likely situated in the lipid polar head group region, and residue 13 is fully inserted into the hydrophobic core of the membrane. © 2013 Vicente et al.

Sobre a implantação de boas práticas de distribuição de produtos médicos de acordo com a RDC nº 59 de 27 de junho de 2000 - ANVISA e NBR ISO 9001:2000

Pós-graduação em Ciências Farmacêuticas - FCFAR

Detecção de genes de beta-lactamases de amplo espectro em Pseudomonas aeruginosa resistentes aos carbapenêmicos isoladas em São José do Rio Preto - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Detecção e identificação dos genes de beta-lactamases blaSHV, blaTEM e blaCTX-M em Klebsiella penumoniae isoladas em um Hospital Terciário do Estado de São Paulo

Pós-graduação em Microbiologia - IBILCE

Notas de la CEPAL Nº 59

América Latina corre el riesgo de perder lo ganado en la reducción de la pobrezaCrisis financiera internacional afectará comercio de la región con el mundo¿;Época de cambios o cambio de época? Columna de opinión de la Secretaria Ejecutiva de la CEPAL, Alicia BárcenaPrecisiones. El vaso medio lleno de los jóvenes latinoamericanosIndicadoresLa estructura tributaria de América Latina y el Caribe contribuye con la desigualdadPublicaciones recientesCalendario de eventos

Notas de Población Vol.22 N° 59

La mujer en el trabajo y la migración. El mercado laboral femenino entre 1950 y 1990 y la inmigración de mujeres a la ciudad de Santiago de Chile / Ivonne Szasz. -- Una visión sintética del ajuste económico y sus consecuencias demográficas en América Latina / Reynaldo F. Bajraj y Jorge Bravo. -- Diferenciales de mortalidad adulta en Argentina / Rafael Rofman. -- La población indígena en los censos de América Latina / Alexia Peyser y Juan Chackiel. -- Discontinuidades demográficas en Brasil y el Estado de Sáo Paulo / Alicia Bercovich y Felicia Madeira. -- Análisis preliminar acerca de las madres solteras, jefas de hogar, en Brasil durante 1970 y 1980 / Susan de Vos. -- Modelación y proyección de la mortalidad en Chile / Ronald D. Lee y Rafael Rofman.