388 resultados para COFFEA-ARABICA


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Ciências Biológicas (Genética) - IBB

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Pós-graduação em Agronomia (Irrigação e Drenagem) - FCA

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

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Obesity along with overweight, has been considered one of the most serious public health problems in the world, especially because they are the main risk factors for many chronic diseases, such as coronary heart disease, type II diabetes mellitus, hypertension and some types of tumors, which are associated with high mortality rates. The use of functional foods and appropriate diets to call health promotion has grown as a mechanism for prevention, control and treatment of chronic diseases such as obesity. Several ethnopharmacological studies indicate plant species for the treatment of disorders associated with obesity with a major attraction of this regimen is perceived as safer and more effective health than the traditional treatment with appetite suppressants. This work aims to develop and standardize models of biological assays in stress and obesity, also aims to evaluate the effect of oil green beans of Coffea arabica in the regulation of body weight and energy balance in mice. To this end, trials were made in vivo studies of this product and their effects on energy metabolism in non-obese mice with obesity induced by hypercaloric diet. After induction of obesity by 8 weeks, animals were treated for 21 days with the extracts orally. After 21 days the animals were killed to evaluate the effects of these products on daily feed intake and on body weight. The group treated with the oil of Coffea arabica L. showed significant weight loss and feed intake high. According to the results, we conclude that the standardized extract of Coffea arabica L. decreased body weight without restriction or decrease the amount of food ingested

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The Coffee Genome Project made available to the scientific community relevant information that made practical the identification and cloning of important genes, as well as the identification of the major sequences involved on their regulation. The aim of the present study was to amplify, clone and sequence coffee promoters with specific expression patterns. For that, coffee ESTs which known expression profiles were employed. First, the promoter regions of coffee genes showing, respectively, fruitspecific and ubiquitous expression were amplified using the Genome Walking strategy. Amplified sequences were then inserted in the pGEM-Teasy vector (Promega) and sequenced. Once completed the sequencing, an expression cassette was constructed using the binary vector pCAMBIA-1381z (Cambia). These expression cassettes were cloned into Agrobacterium tumefaciens, and transgenic tobacco plants were generated aiming the functional characterization of these promoters

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Dentre as várias espécies de café, Coffea arabica L. destaca-se pela sua ampla importância comercial relacionada ao consumo da bebida e à produção de óleo. O grão de café possui grande quantidade de compostos fenólicos bioativos - destacando-se os com atividade antioxidante e fotoprotetora - e após a extração do óleo há sobra de grande quantidade de subprodutos, denominado torta. O objetivo desse trabalho é produzir um extrato a partir da torta de café selecionando o melhor solvente extrator, avaliando o teor de compostos fenólicos e o potencial antioxidante, para o emprego como ativo cosmético em formulações desenvolvidas, com análise in vitro para quantificação do Fator de Proteção Solar (FPS) dessas. Os extratos foram previamente produzidos por sonicação utilizando solventes de diferentes polaridades, e o extrato escolhido foi produzido em maior escala por maceração e realizado também extração exaustiva com o solvente escolhido (extrato etanólico 70%, v/v). Na determinação do teor de fenólicos totais foi utilizado o método com o reagente Folin-Ciocalteu. Foi avaliado também o rendimento (m/m) dos extratos e atividade antioxidante pelo método do radical DPPH. O extrato apresentou boa atividade antioxidante e alto teor de compostos fenólicos em comparação com outras plantas consideradas referências nessas propriedades. Para o preparo das formulações foram utilizados matérias primas aprovadas pela ANVISA, e as quantidades dos filtros solares químicos empregados para obtenção de Fator de Proteção Solar (FPS) teórico próximo de 30, utilizou-se como ferramenta o BASF - Sunscreen Simulator. As formulas desenvolvidas e preparadas foram analisadas com o equipamento Optometrics SPF 290S, capaz de fazer a determinação do FPS por análise in vitro. A preparação selecionada foi a que apresentou FPS adequado pela análise in vitro e sensorial mais leve, visto ...

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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An interdisciplinary study was conducted to evaluate the effects of drying and storage time on changes in the quality of natural and fully washed coffees beans dried out in the yard and mechanically dried at a temperature of 60/40°C in air dryer machine. The coffee beans (Coffea arabica L.) harvested in cherries were processed by dry and wet methods, being subjected to pre-drying yard, followed by drying yard in the sun with air heated of 60/40°C until it reached the water content of 11% (wb). After reached the thermal equilibrium with the environment, the beans were packed in jute bag with a capacity of five kilograms and stored in uncontrolled environment during the period of one year, and removing material from each treatment every three months. To characterize the effect of drying and storage time on the coffee quality different methodologies was evaluated. It was observed less drying time for the fully washed coffee 60/40°C, and thus less energy consumed in the drying process until the point of storage, for the natural coffee there was significant effect of time on the chemical quality, biochemical and sensory; fully washed coffee proved to be more tolerant to drying than natural coffee, regardless of drying method, showing a better drink quality and less variation in chemical composition and biochemistry.