Field applications of the second-generation Environmental Sample Processor (ESP) for remote detection of harmful algae: 2006-2007

We assess the application of the second-generation Environmental Sample Processor (ESP) for the detection of harmful algal bloom (HAB) species in field and laboratory settings using two molecular probe techniques: a sandwich hybridization assay (SHA) and fluorescent in situ hybridization (FISH). During spring 2006, the first time this new instrument was deployed, the ESP successfully automated application of DNA probe arrays for various HAB species and other planktonic taxa, but non-specific background binding on the SHA probe array support made results interpretation problematic. Following 2006, the DNA array support membrane that we were using was replaced with a different membrane, and the SHA chemistry was adjusted. The sensitivity and dynamic range of these modifications were assessed using 96-well plate and ESP array SHA formats for several HAB species found commonly in Monterey Bay over a range of concentrations; responses were significantly correlated (p < 0.01). Modified arrays were deployed in 2007. Compared to 2006, probe arrays showed improved signal:noise, and remote detection of various HAB species was demonstrated. We confirmed that the ESP and affiliated assays can detect HAB populations at levels below those posing human health concerns, and results can be related to prevailing environmental conditions in near real-time.

Distribution of calcifying and silicifying phytoplankton in relation to environmental and biogeochemical parameters during the late stages of the 2005 North East Atlantic Spring Bloom

The late stage of the North East Atlantic (NEA) spring bloom was investigated during June 2005 along a transect section from 45 to 66 degrees N between 15 and 20 degrees W in order to characterize the contribution of siliceous and calcareous phytoplankton groups and describe their distribution in relation to environmental factors. We measured several biogeochemical parameters such as nutrients, surface trace metals, algal pigments, biogenic silica (BSi), particulate inorganic carbon (PIC) or calcium carbonate, particulate organic carbon, nitrogen and phosphorus (POC, PON and POP, respectively), as well as transparent exopolymer particles (TEP). Results were compared with other studies undertaken in this area since the JGOFS NABE program. Characteristics of the spring bloom generally agreed well with the accepted scenario for the development of the autotrophic community. The NEA seasonal diatom bloom was in the late stages when we sampled the area and diatoms were constrained to the northern part of our transect, over the Icelandic Basin (IB) and Icelandic Shelf (IS). Coccolithophores dominated the phytoplankton community, with a large distribution over the Rockall-Hatton Plateau (RHP) and IB. The Porcupine Abyssal Plain (PAP) region at the southern end of our transect was the region with the lowest biomass, as demonstrated by very low Chla concentrations and a community dominated by picophytoplankton. Early depletion of dissolved silicic acid (DSi) and increased stratification of the surface layer most likely triggered the end of the diatom bloom, leading to coccolithophore dominance. The chronic Si deficiency observed in the NEA could be linked to moderate Fe limitation, which increases the efficiency of the Si pump. TEP closely mirrored the distribution of both biogenic silica at depth and prymnesiophytes in the surface layer suggesting the sedimentation of the diatom bloom in the form of aggregates, but the relative contribution of diatoms and coccolithophores to carbon export in this area still needs to be resolved.

Avaliação dos impactos econômicos, sociais e ambientais da cultivar de milho BR 201 na safra 2005/2006.

2007

Avaliação dos impactos econômicos, sociais e ambientais da cultivar de sorgo granífero BR 304 na safra 2005/2006.

2007

Informes da avaliação de genótipos de girassol 2005/2006 e 2006.

Características gerais da Rede Oficial de Avaliação de Genótipos de Girassol; Resultados dos ensaios finais de primeiro e segundo ano; Lista dos genótipos de girassol avaliados e registrados no Ministério da Agricultura, Pecuária e Abastecimento - MAPA.

Avaliação de genótipos de girassol na região do Planalto Médio do Rio Grande do Sul na safra 2005/2006.

2007

Relatório Técnico e de Atividades 2005/2006.

2006

Proceedings : Grand Lodge, A.F. & A.M. of Canada in the Province of Ontario - 2005

On spine : The Grand Lodge of Canada in the Province of Ontario.

Rapport annuel 2005-2006

Rapport annuel des activités de la Direction des bibliothèques de l'Université de Montréal en 2005-2006.

Actuaciones de educación para la salud y prevención de drogodependencias en centros educativos de Cartagena : curso 2005-2006.

Se incluyen fichas de solicitud y un informe sobre los resultados de la aplicación de los materiales didácticos elaborados para el curso 2004-2005. Publicación periódica anual desde el 2001 (curso 2001-2002)