Desenvolvimento e validação de um novo protocolo para ciclagem térmica de materiais dentários

Pós-graduação em Odontologia Restauradora - ICT

Produção do espaço e controle social: os espaços residenciais populares fechados como novo modelo de moradia. -

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

De novo transcriptome assembly for a non-model species, the blood-sucking bug Triatoma brasiliensis, a vector of Chagas disease

High throughput sequencing (HTS) provides new research opportunities for work on non-model organisms, such as differential expression studies between populations exposed to different environmental conditions. However, such transcriptomic studies first require the production of a reference assembly. The choice of sampling procedure, sequencing strategy and assembly workflow is crucial. To develop a reliable reference transcriptome for Triatoma brasiliensis, the major Chagas disease vector in Northeastern Brazil, different de novo assembly protocols were generated using various datasets and software. Both 454 and Illumina sequencing technologies were applied on RNA extracted from antennae and mouthparts from single or pooled individuals. The 454 library yielded 278 Mb. Fifteen Illumina libraries were constructed and yielded nearly 360 million RNA-seq single reads and 46 million RNA-seq paired-end reads for nearly 45 Gb. For the 454 reads, we used three assemblers, Newbler, CAP3 and/or MIRA and for the Illumina reads, the Trinity assembler. Ten assembly workflows were compared using these programs separately or in combination. To compare the assemblies obtained, quantitative and qualitative criteria were used, including contig length, N50, contig number and the percentage of chimeric contigs. Completeness of the assemblies was estimated using the CEGMA pipeline. The best assembly (57,657 contigs, completeness of 80 %, < 1 % chimeric contigs) was a hybrid assembly leading to recommend the use of (1) a single individual with large representation of biological tissues, (2) merging both long reads and short paired-end Illumina reads, (3) several assemblers in order to combine the specific advantages of each.

Autism spectrum disorder in a girl with a de novo x;19 balanced translocation

Balanced X-autosome translocations are rare, and female carriers are a clinically heterogeneous group of patients, with phenotypically normal women, history of recurrent miscarriage, gonadal dysfunction, X-linked disorders or congenital abnormalities, and/or developmental delay. We investigated a patient with a de novo X;19 translocation. The six-year-old girl has been evaluated due to hyperactivity, social interaction impairment, stereotypic and repetitive use of language with echolalia, failure to follow parents/caretakers orders, inconsolable outbursts, and persistent preoccupation with parts of objects. The girl has normal cognitive function. Her measurements are within normal range, and no other abnormalities were found during physical, neurological, or dysmorphological examinations. Conventional cytogenetic analysis showed a de novo balanced translocation, with the karyotype 46,X,t(X;19)(p21.2;q13.4). Replication banding showed a clear preference for inactivation of the normal X chromosome. The translocation was confirmed by FISH and Spectral Karyotyping (SKY). Although abnormal phenotypes associated with de novo balanced chromosomal rearrangements may be the result of disruption of a gene at one of the breakpoints, submicroscopic deletion or duplication, or a position effect, X; autosomal translocations are associated with additional unique risk factors including X-linked disorders, functional autosomal monosomy, or functional X chromosome disomy resulting from the complex X-inactivation process.

Comparing de novo and reference-based transcriptome assembly strategies by applying them to the blood-sucking bug Rhodnius prolixus

High Throughput Sequencing capabilities have made the process of assembling a transcriptome easier, whether or not there is a reference genome. But the quality of a transcriptome assembly must be good enough to capture the most comprehensive catalog of transcripts and their variations, and to carry out further experiments on transcriptomics. There is currently no consensus on which of the many sequencing technologies and assembly tools are the most effective. Many non-model organisms lack a reference genome to guide the transcriptome assembly. One question, therefore, is whether or not a reference-based genome assembly gives better results than de novo assembly. The blood-sucking insect Rhodnius prolixus-a vector for Chagas disease-has a reference genome. It is therefore a good model on which to compare reference-based and de novo transcriptome assemblies. In this study, we compared de novo and reference-based genome assembly strategies using three datasets (454, Illumina, 454 combined with Illumina) and various assembly software. We developed criteria to compare the resulting assemblies: the size distribution and number of transcripts, the proportion of potentially chimeric transcripts, how complete the assembly was (completeness evaluated both through CEGMA software and R. prolixus proteome fraction retrieved). Moreover, we looked for the presence of two chemosensory gene families (Odorant-Binding Proteins and Chemosensory Proteins) to validate the assembly quality. The reference-based assemblies after genome annotation were clearly better than those generated using de novo strategies alone. Reference-based strategies revealed new transcripts, including new isoforms unpredicted by automatic genome annotation. However, a combination of both de novo and reference-based strategies gave the best result, and allowed us to assemble fragmented transcripts.

Lei de Meios argentina: entre o velho e o novo na disputa pelo direito à comunicação

A pesquisa analisa o novo marco regulatório da comunicação da Argentina, a Lei n° 26.522/2009 de Serviços de Comunicação e Audiovisual (LSCA). O texto é referência reguladora emblemática e contemporânea para os campos de estudos da Comunicação, das Políticas Públicas e da Economia Política da Comunicação na América Latina. O caráter original dessa legislação reside na aproximação de uma concepção inovadora do direito à comunicação e na atribuição de um novo papel regulador reservado ao Estado. A criação de uma nova arquitetura institucional para o desenho das políticas de comunicação, o estabelecimento de limites de propriedade para empresas e grupos da radiodifusão e a divisão do espectro radioelétrico em partes iguais para operadores privados, estatais e privados sem fins de lucro são objetos de especial atenção. Nesse sentido, são analisadas as premissas normativas da LSCA e os obstáculos à sua implantação. O processo de elaboração e implantação dessa nova legislação é inseparável do momento político e da correlação de forças políticas do país e de um fenômeno comum na América Latina desde a redemocratização: a emergência da sociedade civil e a luta pela difusão dos direitos de cidadania. A pesquisa aponta tensões entre politicas públicas progressistas e velhos costumes impregnados no Estado, como o fortalecimento de grupos de mídia próximos ao governo de turno e o uso oficialista dos canais de comunicação estatais. Assim, a LSCA revela baixo grau de materialização, a despeito de representar um avanço normativo, institucional e político chave para o país e a região na perspectiva da democratização da comunicação

A escrita para percussão dos compositores do Grupo Música Nova: a busca pelo novo analisada a partir da notação

Pós-graduação em Música - IA