Täystehokonvertterin tehohäviöt ja hyötysuhde

Diplomityössä perehdytään tuuliturbineissa käytettyjen täystehokonvertterien tehohäviöihin ja hyötysuhteeseen. Täystehokonvertterissa generaattorin tuottama sähköteho tasasuunnataan hyvällä hyötysuhteella aktiivisella geenraattorisillalla konvertterin välipiiriin ja edelleen vaihtosuunnataan aktiivisella verkkovaihtosuuntaajasillalla siirtoverkkoon. Työn tarkoituksena on antaa yleiskuva tehohäviöiden jakautumisesta ja yksinkertaistaa niiden laskentaa. Häviömekanismit ja häviöiden määräytyminen esitellään pääkomponenttitasolla. Tehohäviöiden osalta keskitytään erityisesti muuntajateräksestä valmistettujen sinisuotimien ja du/dt-suotimien rautahäviöihin. Ongelmana rautahäviöiden määrittämisessä on korkeilla taajuuksilla tapahtuvat häviöt, joiden laskentaan ei ole yleensä saatavilla tarvittavia materiaaliparametrejä. Tehohäviöiden laskentaa varten toteutettu laskentasovellus on esitelty periaatteellisina vuokaavioina ja sovelluksella saatavia tuloksia on esitetty ja vertailtu mitattuihin tuloksiin.

Separation of Gas Bubbles from Circulation Waters and Pulp Suspensions in Open Channel Flow

The objective of this thesis was to study the removal of gases from paper mill circulation waters experimentally and to provide data for CFD modeling. Flow and bubble size measurements were carried out in a laboratory scale open gas separation channel. Particle Image Velocimetry (PIV) technique was used to measure the gas and liquid flow fields, while bubble size measurements were conducted using digital imaging technique with back light illumination. Samples of paper machine waters as well as a model solution were used for the experiments. The PIV results show that the gas bubbles near the feed position have the tendency to escape from the circulation channel at a faster rate than those bubbles which are further away from the feed position. This was due to an increased rate of bubble coalescence as a result of the relatively larger bubbles near the feed position. Moreover, a close similarity between the measured slip velocities of the paper mill waters and that of literature values was obtained. It was found that due to dilution of paper mill waters, the observed average bubble size was considerably large as compared to the average bubble sizes in real industrial pulp suspension and circulation waters. Among the studied solutions, the model solution has the highest average drag coefficient value due to its relatively high viscosity. The results were compared to a 2D steady sate CFD simulation model. A standard Euler-Euler k-ε turbulence model was used in the simulations. The channel free surface was modeled as a degassing boundary. From the drag models used in the simulations, the Grace drag model gave velocity fields closest to the experimental values. In general, the results obtained from experiments and CFD simulations are in good qualitative agreement.

The use of fluorescent probes to assess viability of the plant pathogenic bacterium Clavibacter michiganensis subsp. michiganensis by flow cytometry

Determination of the viability of bacteria by the conventional plating technique is a time-consuming process. Methods based on enzyme activity or membrane integrity are much faster and may be good alternatives. Assessment of the viability of suspensions of the plant pathogenic bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) using the fluorescent probes Calcein acetoxy methyl ester (Calcein AM), carboxyfluorescein diacetate (cFDA), and propidium iodide (PI) in combination with flow cytometry was evaluated. Heat-treated and viable (non-treated) Cmm cells labeled with Calcein AM, cFDA, PI, or combinations of Calcein AM and cFDA with PI, could be distinguished based on their fluorescence intensity in flow cytometry analysis. Non-treated cells showed relatively high green fluorescence levels due to staining with either Calcein AM or cFDA, whereas damaged cells (heat-treated) showed high red fluorescence levels due to staining with PI. Flow cytometry also allowed a rapid quantification of viable Cmm cells labeled with Calcein AM or cFDA and heat-treated cells labeled with PI. Therefore, the application of flow cytometry in combination with fluorescent probes appears to be a promising technique for assessing viability of Cmm cells when cells are labeled with Calcein AM or the combination of Calcein AM with PI.