I Curso de Educação Popular na Universidade de Évora

I Curso de Educação Popular, realizado no Colégio do Espírito Santo da Universidade de Évora, a 22 de Março de 2016.

Study of the allergenic profile of pollen from Platanus hybrida in the city of Evora, Portugal

Background and Aim: Although grasses and olive are the most relevant allergenic species in the Alentejo region, aggravation of allergic symptoms in the early spring, unrelated with those species pollen seasons, has been reported, particularly in urban environment. Plane trees, hence pollen, are highly abundant in the city of Évora, nonetheless allergen pollen profile has not yet been evaluated. The aim of this work was to characterize the allergen profile of pollen from Platanus hybrida, one of the most representative species in Evora showing pollination prior to the main pollen season in Alentejo. Methods: Pollen from Platanus hybrida and Dactylis glomerata was extracted with ammonium bicarbonate buffer, lyophilized and stored at -80ºC until analysis. Protein content was determined by the Bradford method. SDS-PAGE followed by western blot, using allergic patient sera (obtained from the Hospital do Espírito Santo de Évora – HESE), were performed to evaluate the allergen profile of the pollen. Sensitization and cross-reactivity was assessed by solid phase immunoblot. Results: Half of the patient exhibited sensitization to pollen extracts of P. Hybrida. Western blot have shown several immunoreactive bands in the Mr 10-90 kDa range. Immunoreactive bands were also observed in the protein profile according to the pI in the pI range 4.0-6.1. Cross-reactivity of P. hybrida with D. glomerata was found. Although several bands are common to D. glomerata, a band with ~50kDa was observed in P. hybrida but not in D. glometata. Conclusion: These results evidenced allergens found in P. hybrida pollen. Moreover, cross–reactivity between P. hybrida and highly allergenic species such as D. glomerata was found which probably contributes for aggravation of pollinosis in the early spring. Acknowledgments: This work was supported by FEDER through the “Programa Operacional Fatores de Competitividade – COMPETE” (Strategic projects of ICAAM and ICT 2013-2015).

Allergenic profile of Quercus rotundifolia pollen in Alentejo, Portugal

Background and Aim: Grasses and olive are the most relevant allergenic species in the Alentejo region. However, aggravation of allergic symptoms has been reported in the early spring, before grass and olive pollen seasons. Quercus pollen is the most abundant pollen type in the early spring in Alentejo, nonetheless its allergen profile has not yet been evaluated. The aim of this work was to characterize the allergen profile of pollen from Quercus rotundifolia among the most representative species showing pollination in April, prior to the main pollen season in Alentejo. Methods: Pollen from Quercus rotundifolia, Olea europaea and Dactylis glomerata was extracted with ammonium bicarbonate buffer, lyophilized and stored at -80ºC until analysis. Extract from Quercus ilex pollen was kindly offered by Bial. Protein content was determined by the Bradford method. SDS-PAGE followed by western blot, using allergic patient sera (obtained from the Hospital do Espírito Santo de Évora – HESE), were performed to evaluate the allergen profile of the pollen. Sensitization and cross-reactivity was assessed by solid phase immunoblot. Results: Most of the patient evidenced sensitization to pollen extracts of Q. rotundifolia. Protein profile of Q. rotundifolia has shown several bands in the Mr 10-90 kDa, mostly overlapping with Q. ilex. Western blot have shown several immunoreactive bands. Immunoreactive bands were also observed in the protein profile according to the pI in the range 4.0-6.1. Cross-reactivity between Q. rotundifolia with O. europaea and D. glomerata was found. Conclusion: These results evidenced allergens found in Q. rotundifolia pollen. It also shows that protein profile of Q. rotundifolia and Q. ilex are mostly alike suggesting that similarities in allergen profile are expected. Moreover, cross–reactivity between Q. rotundifolia and highly allergenic species such as O. europaea and D. glomerata was found which probably contributes to the aggravation of pollinosis in the early spring. Acknowledgments: This work was supported by FEDER through the “Programa Operacional Fatores de Competitividade – COMPETE” (Strategic projects of ICAAM and ICT 2013-2015). We also aknowledge Bial-Aristegui for supplying pollen and extract samples of Q. ilex.

Plano de marketing da L. Nostrum, S.A. para os anos de 2010 e 2011

No actual contexto empresarial, com uma competitividade e complexidade dos mercados crescente, o marketing tem ganho uma grande relevância na direcção e desenvolvimento das empresas, independentemente do sector onde actuem. As áreas da geriatria e dos serviços de saúde não são excepção, apesar de por vezes nas pequenas e médias empresas, não estarem claramente identificadas todas as políticas e estratégias de marketing que deverão pautar as decisões a tomar. Toma-se então não só importante, como de extrema necessidade, que tal como as decisões financeiras, também as decisões de marketing sejam tomadas de forma explícita e estratégica, de forma que se clarifique no interior da organização o rumo a seguir. Este trabalho irá incidir sobre o marketing de uma empresa que desenvolve a sua actividade num ramo de negócio emergente em Portugal, os Cuidados Continuados Integrados e as Residências Assistidas, tendo como objectivo a sustentabilidade e expansão da organização. Como acontece com a maior parte das empresas de pequena dimensão, a L Nostrum S.A. não dispõe de um plano de marketing, sendo de extrema importância que se proceda à elaboração e implementação de um projecto desta natureza Assim, a proposta que aqui se apresenta têm como objectivo central o desenvolvimento de um plano de marketing de curto prazo para a empresa L Nostrum S.A. ABSTRACT: ln the actual business context, with growmg market competitiveness and complexity, marketing has been getting higher relevance in company development, regardless the sector in which these companies operate. The areas of geriatrics and healthcare aren't an exception, although sometimes in some SME's the marketing strategies that shall regulate some of the company's decisions, aren't clearly defined. So, it becomes not only important, as a dire need, that, just like financial decisions, also marketing decisions, are made in an explicit and strategic way, so it becomes clear the path that the organization should follow. This work will focus on the marketing campaign of a company which acts on a business branch emerging in Portugal, the Integrated Continuous Care and Assisted Living Facilities, having as its objective the sustainability and expansion of this organization. Like most small companies, L Nostrum S.A., does not have a marketing plan, being of extreme significance the elaboration and implementation of a project of this nature. So, the proposal here presented has as its main objective the development of a short term marketing plan for the company L Nostrum S.A.

Perfil em alergénios do pólen de Quercus rotundifólia

Background and Aim: Grasses and olive are the most relevant allergenic species in the Alentejo region. However, aggravation of allergic symptoms has been reported in the early spring, before grass and olive pollen seasons. Quercus pollen is the most abundant pollen type in the early spring in Alentejo, nonetheless its allergen profile has not yet been evaluated. The aim of this work was to characterize the allergen profile of pollen from Quercus rotundifolia the most representative species showing pollination in April, prior to the main pollen season in Alentejo. Methods: Pollen from Quercus rotundifolia, Olea europaea and Dactylis glomerata was extracted with ammonium bicarbonate buffer, lyophilized and stored at -80ºC until analysis. Extract from Quercus ilex pollen was kindly offered by Bial. Protein content was determined by the Bradford method. SDS-PAGE followed by western blot, using allergic patient sera (obtained from the Hospital do Espírito Santo de Évora – HESE), were performed to evaluate the allergen profile of the pollen. Results: Protein profile of Q. rotundifolia has shown several bands in the Mr 10-90 kDa, mostly overlapping with Q. ilex. Western blot have shown several immunoreactive bands. Protein profile according to the pI showed immunoreactive bands in the pI range 4.0-6.1. Cross-reactivity of Q. rotundifolia with O. europaea and D. glomerata was found. Conclusion: These results evidenced allergens found in Q. rotundifolia pollen. It also shows that protein profile of Q. rotundifolia and Q. ilex are mostly alike suggesting that similarities in allergen profile are expected. Moreover, cross–reactivity between Q. rotundifolia and highly allergenic species such as O. europaea and D. glomerata was found which probably contributes for aggravation of pollinosis in the early spring. Acknowledgments: This work was supported by “FEDER - Programa Operacional Factores de Competitividade – COMPETE”. A special acknowledgment to our colleague Prof. Rui Brandão, deceased, for his dedication to the present work, to the implantation and development of Aerobiology in the University of Évora. We have the honour of dedicating this work to the memory of Prof. Rui Brandão.

Physical soil attributes of Conilon coffee (Coffea canephora) under organic and conventional management systems.

Coffea sp. is cultivated in large areas, using both conventional and organic management. However, information about the sustainability of these two management systems is still deficient. The objective of the present study was to evaluate the physical properties of soil cultivated with Conilon coffee (C. canephora) under organic and conventional management. Two areas cultivated with Conilon coffee (under organic and conventional management) and a fragment of Atlantic forest, used as a reference, were selected for the experiment. Soil granulometry, hydraulic conductivity, water retention curve, resistance to penetration, porosity, optimal hydric interval, and other physical characteristics were measured at depths of 0 to 10 and 10 to 20 cm. The data was submitted to multivariate and descriptive statistical analyses. Higher similarity was observed between the soil cultivated with Conilon coffee under organic management and the Atlantic forest soil. Soil resistance to penetration at 10, 30, 100, 500 and 1500 kPa, macro porosity, density and total porosity were the main physical properties that differentiated both management systems studied. The non-use of agricultural machinery and the addition of organic matter may be the main reasons for higher soil sustainability observed under organic management when compared with the conventional system.

Divergência genética em genótipos de maracujazeiro azedo, com base em características físicas e químicas dos frutos.

RESUMO: Objetivou-se com o presente trabalho avaliar a divergência genética e as características físicas e químicas de frutos de duas populações do maracujazeiro azedo na região Norte do Espírito Santo, como as progênies de meio-irmãos de acesso local de um plantio comercial (genótipos: 1; 2; 3; 4; 5; 6; 7; 8; 9 e 10) e do híbrido BRS Ouro Vermelho (genótipos: 11; 12; 13; 14; 15; 16; 17; 18; 19 e 20). A divergência genética foi avaliada por procedimentos multivariados como a distância generalizada de Mahalanobis (D2) e pelos métodos de agrupamento de otimização de Tocher e UPGMA. Encontrou-se divergência genética entre as populações estudadas promovendo a formação de grupos diferentes entre o método de Tocher e do UPGMA. As características, referentes ao tamanho do fruto, diâmetro polar e equatorial, foram as que mais contribuíram na diversidade genética dos genótipos. Nas populações estudadas de maracujazeiro azedo há grande variabilidade genética quanto às características avaliadas, o que possibilita selecionar plantas com elevado potencial para fins de melhoramento genético. O híbrido BRS Ouro Vermelho apresenta boa adaptação às condições locais. ABSTRACT: The aim of the present work was to evaluate genetic divergence and physical and chemical characteristics in fruit of two populations of sour passion fruit in the northern region of the State of Espírito Santo, Brazil, these being half-sibling progenies from local accessions of a commercial crop (genotypes 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10) and the hybrid BRS Ouro Vermelho (genotypes: 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20). Genetic divergence was evaluated using such multivariate procedures as the generalised Mahalanobis distance (D2) and the Tocher optimisation and UPGMA clustering methods. Genetic divergence was found between the populations under study, promoting the formation of different groups between the Tocher and UPGMA methods. As characteristics for fruit size, the polar and equatorial diameters had the most impact on the genetic diversity of the genotypes. In the populations of sour passion fruit being studied, great genetic variability is seen in the evaluated characteristics, making it possible to select plants of high potential for breeding purposes. The BRS Ouro Vermelho hybrid is well adapted to the local conditions.

O ESPAÇO URBANO DE VILA VELHA-ES E A SEGREGAÇÃO SÓCIO ESPACIL ENTRE 1970 E 2010

Demonstra os variados tipos de deficiência visuais existentes, desde a baixa visão, até a cegueira, classificando cada uma delas. Propõe o uso de oficinas pedagógicas para o aprimoramento do processo de ensino-aprendizagem de alunos deficientes visuais, melhorando a prática pedagógica de professores com pouca experiência e dando acesso a educação de direito aos deficientes visuais. Diagnostica, através de entrevistas, os problemas enfrentados por instituições que dão suporte aos deficientes visuais, bem como as dificuldades enfrentadas pelos mesmos no ensino de Geografia. Desenvolve uma oficina pedagógica, baseada nas dificuldades diagnosticadas em entrevistas realizadas com deficientes visuais. Produz recursos didáticos – mapa e globo tátil – com materiais diferenciados (miçangas, tecidos, papéis importados), a fim de levar aos deficientes visuais a compreensão de localização, sendo esta, uma iniciação cartográfica de que eles tanto urgem. Conclui que as dificuldades enfrentadas pelos deficientes visuais nas escolas de ensino regular são muitas e que há falta de formação para os profissionais que se relacionam com esses indivíduos.

ARTICULAÇÃO DO SEMIÁRIDO BRASILEIRO (ASA): ALTERNATIVAS DESENVOLVIMENTISTAS PARA O SEMIÁRIDO DO BRASIL

O semiárido brasileiro abrange o norte dos Estados de Minas Gerais e Espírito Santo, parte do Sudeste do Maranhão, além dos sertões de Sergipe, Alagoas, Bahia, Pernambuco, Ceará, Paraíba e Rio Grande do Norte. O mesmo apresenta chuvas irregulares, áreas áridas e em risco de desertificação, afetadas por períodos longos de seca. Estes aspectos por sua vez contribuem para a ocorrência de baixos indicadores socioeconômicos da região semiárida. Diante do exposto, o presente trabalho discorre acerca da atuação da Articulação no Semiárido Brasileiro (ASA), fórum de organizações da sociedade civil, que integra diversas entidades, sindicatos, trabalhadores rurais, Organizações Não Governamentais - ONGs, entre outras, operando em busca de transformações socioeconômicas, políticas e culturais para o semiárido. Nessa perspectiva, este trabalho objetiva compreender a atuação da Articulação do Semiárido Brasileiro (ASA) no Brasil. E o caminho trilhado para alcançar tal objetivo se pauta em revisão bibliográfica e análise de informações do Fórum de organizações (ASA). O resultado consiste em análise das ações realizadas pela mesma nos últimos anos e os benefícios trazidos para estas regiões.

OUTROS OLHARES GEOGRÁFICOS (UM ESTUDO COM APRENDIZAGENS GEOGRAFICAS DE ALUNOS PORTADORES DE DEFICIÊNCIAS VISUAIS)

Demonstra os variados tipos de deficiência visuais existentes, desde a baixa visão, até a cegueira, classificando cada uma delas. Propõe o uso de oficinas pedagógicas para o aprimoramento do processo de ensino-aprendizagem de alunos deficientes visuais, melhorando a prática pedagógica de professores com pouca experiência e dando acesso a educação de direito aos deficientes visuais. Diagnostica, através de entrevistas, os problemas enfrentados por instituições que dão suporte aos deficientes visuais, bem como as dificuldades enfrentadas pelos mesmos no ensino de Geografia. Desenvolve uma oficina pedagógica, baseada nas dificuldades diagnosticadas em entrevistas realizadas com deficientes visuais. Produz recursos didáticos – mapa e globo tátil – com materiais diferenciados (miçangas, tecidos, papéis importados), a fim de levar aos deficientes visuais a compreensão de localização, sendo esta, uma iniciação cartográfica de que eles tanto urgem. Conclui que as dificuldades enfrentadas pelos deficientes visuais nas escolas de ensino regular são muitas e que há falta de formação para os profissionais que se relacionam com esses indivíduos.