999 resultados para PROTEINA BRUTA


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The objective of this work was to determine nutrient deposition on the carcass of bullfrog (Lithobates catesbeianus) tadpoles using a nonlinear model. A total of 2,700 tadpoles with an average weight of 0.039 g were used. Commercial ground feed containing 55% crude protein was offered ad libitum. The animals were weighed and evaluated every ten days for analysis of crude protein, ether extract, water, and mineral salt contents. The parameters of the Gompertz model were estimated by the modified Gauss-Newton method, and the deposition rates (g per day) over time were calculated by the resulting equation. The values found for the parameters of the Gompertz equation, used to describe nutrient deposition on tadpole carcass, showed biological interpretation. Maximum deposition rate (t*) was observed on the 36.2331th day for protein, on the 37.1420th day for water, on the 35.2971th day for mineral salt, and on the 41.3547th day for fat. Nutrient intake from the diet is higher than the deposition rate on the tadpole carcass.

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Objetivou-se avaliar o consumo e a digestibilidade total e parcial de dietas utilizando milho, casca de soja e polpa cítrica associados ao farelo de girassol ou ureia em novilhos confinados. Foram utilizados quatro novilhos ½ Aberdeen Angus vs. ½ Nelore, providos de cânulas no rúmen e duodeno, com 18 meses de idade e 360 kg de peso médio inicial. As dietas foram constituídas por duas fontes energéticas (milho grão e polpa cítrica) associadas a duas fontes nitrogenadas (ureia e farelo de girassol), totalizando quatro tratamentos, numa proporção volumoso:concentrado de 40:60. O delineamento utilizado foi o quadrado latino com quatro tratamentos e quatro períodos de 21 dias cada. O consumo médio diário de matéria seca foi influenciado (p < 0,05) entre as dietas, e o maior valor foi atribuído à dieta milho e farelo de girassol. A digestibilidade aparente de todos os componentes, exceto da proteína bruta, foi maior (p < 0,05) para as dietas com polpa cítrica. Não houve efeito (p > 0,05) entre as dietas para as digestibilidades ruminal e intestinal. A utilização de coprodutos na alimentação animal não prejudicou a digestibilidade das dietas, sendo indicados como ingredientes de elevado potencial de utilização em dietas para bovinos de corte.

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We evaluated the effect of adding by-products from the processing of oil seeds in the diet of lambs on the carcass and meat traits. Twenty-four non-castrated weaned male Santa Inês lambs with approximately 70 days of age and initial average weight of 19.11 ± 2.12 kg were distributed into a completely randomized design. Treatments consisted of diets containing by-products with 70% of concentrate and 30% of tifton hay (Cynodon spp.) and were termed SM: control with soybean meal; SC: formulated with soybean cake; SUC: formulated with sunflower cake and PC: formulated with peanut cake. Diets had no effects on the carcass traits evaluated. There was no significant effect on the mean values of perirenal, omental and mesenteric fats (0.267, 0.552 and 0.470 kg, respectively) and there was no influence on the percentages of moisture, ether extract, crude protein or ash in the loin between experimental diets. Diets containing by-products from the processing of oil seeds did not change fatty acids found in lamb meat. The use of by-products from oil seeds provided similar carcass and meat traits, thus their use can be recommended as eventual protein and energy sources for feedlot lambs.

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O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por Western blot. Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Zootecnia - FMVZ

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This study was carried out in Fazenda Canchim in Sao Carlos-SP, physical base of Embrapa Southeast Livestock, aiming to select, based on the agronomic characteristics of productivity of dry matter and quality of the forage, the accesses of suitable gender Paspalum for possessing potentiality of use as foraging plants. Were evaluated for 3 years, with 22 forage grasses, being 19 of Paspalum and three more of control species: Brachiaria decumbens, Andropogon gayanus cv. Baet and Panicum maximum cv. Tanzania, submitted to two intensification levels: high (fertilizer after each cut and supplemental irrigation) and low (only fertilizer of annual replacement without irrigation). The experimental design was of split blocks subdivided in the space and time with two replications. The studied variables were: dry matter production, in vitro dry matter digestibility and crude protein content. The results show that the accesses responded to the intensification level minimizing the negative effect of the stational production. Based on the studied variables, the selected accesses were: BRA-011401 (Paspalum glaucescens); BRA-011681 (P. atratum); BRA009661 (P. atratum) and BRA-019186 (P. regnellii).