A new species of Henneguya (Myxosporida, Protozoa) from West African catfish, Clarias lazera Val., with a review of the genus Henneguya Thelohan, 1892

A new species of myxosporida, Henneguya clariae, is described from the gills of Claria’s Iazera caught from various waters in Nigeria. Pathogenic effects in the destruction of the components of the branchial system are about 25% of Clarias lazera. A review of the genera Henneguya myxobilatus, and Neohenneguya is made and these were synonymised, with some reservations in the case of to Henneguya. A checklist of the genus Henneguya is given.

Experimental observation of von Kármán vortices during drop impact

The observation of von Kármán type vortices during the impact of water droplets onto a pool of water is reported. Shadowgraph imaging and laser-sheet visualization are used to document these events. The appearance of these vortices occurs within theoretically predicted regions in a Reynolds-splash number parameter space. In addition, and also in agreement with theoretical predictions, smooth splashing, with vortices absent, is found for smaller Reynolds number. © 2012 American Physical Society.

Experimental observation of von Kármán vortices during drop impact.

The observation of von Kármán type vortices during the impact of water droplets onto a pool of water is reported. Shadowgraph imaging and laser-sheet visualization are used to document these events. The appearance of these vortices occurs within theoretically predicted regions in a Reynolds-splash number parameter space. In addition, and also in agreement with theoretical predictions, smooth splashing, with vortices absent, is found for smaller Reynolds number.

U19/Eaf2 Binds to and Stabilizes von Hippel-Lindau Protein

Studies have firmly established a key regulatory role for the tumor suppressor pVHL in the regulation of the vascular system and normal spermatogenesis. Here, we report that knockout of the newly identified tumor suppressor U19/Eaf2 also caused vascular system abnormalities and aspermatogenesis, suggesting a potential link between U19/Eaf2 and pVHL. Coimmunoprecipitation and in vitro binding assays showed an association between U19/Eaf2 and pVHL, whereas deletion mutagenesis revealed the requirement of the NH2 terminus of U19/Eaf2 and both the alpha and beta domains of pVHL for this binding. U19/Eaf2 stabilizes pVHL, as shown by protein stability and pulse-chase studies. Testes and mouse embryonic fibroblasts (MEF) derived from U19/Eaf2 knockout mice expressed reduced levels of pVHL, indicating that full in vivo expression of pVHL indeed requires U19/Eaf2. As expected, U19/Eaf2 knockout MEF cells exhibited an increased level and activity of hypoxia-inducible factor 1 alpha (HIF1 alpha), a protein typically regulated via a pVHL-mediated degradation pathway. Furthermore, angiogenesis in a Matrigel plug assay was significantly increased in U19/Eaf2 knockout mice. The above observations argue that U19/Eaf2 can modulate HIF1 alpha and angiogenesis, possibly via direct binding and stabilization of pVHL. [Cancer Res 2009;69(6):2599-606]

Endothelial microparticles released in thrombotic thrombocytopenic purpura express von Willebrand factor and markers of endothelial activation

It has been suggested that endothelial apoptosis is a primary lesion in the pathogenesis of thrombotic thrombocytopenic purpura (TTP). We tested this hypothesis by examining the phenotypic signatures of endothelial microparticles (EMP) in TTP patients. In addition, the effect of TTP plasma on microvascular endothelial cells (MVEC) in culture was further delineated. EMP released by endothelial cells (EC) express markers of the parent EC; EMP released in activation carry predominantly CD54 and CD62E, while those in apoptosis CD31 and CD105. We investigated EMP release in vitro and in TTP patients. Following incubation of MVEC with TTP plasma, EMP and EC were analysed by flow cytometry for the expression of CD31, CD51, CD54, CD62E, CD105, CD106 and von Willebrand factor (VWF) antigen. EMP were also analysed in 12 TTP patients. In both EC and EMP, CD62E and CD54 expression were increased 3- to 10-fold and 8- to 10-fold respectively. However, CD31 and CD105 were reduced 40-60% in EC but increased twofold in EMP. VWF expression was found in 55 +/- 15% of CD62E(+) EMP. Markers of apoptosis were negative. In TTP patients, CD62E(+) and CD31(+)/CD42b(-) EMP were markedly elevated, and preceded and correlated well with a rise in platelet counts and a fall in lactate dehydrogenase. CD62E(+) EMP (60 +/- 20%) co-expressed VWF and CD62E. The ratio of CD31(+)/42b(-) to CD62E(+) EMP exhibited a pattern consistent with activation. In conclusion, our studies indicate endothelial activation in TTP. EMP that co-express VWF and CD62E could play a role in the pathogenesis of TTP.

Zwischen Widerstandsrecht und starkem Staat. Ein Beitrag zur deutschen Rezeptionsgeschichte von Hobbes

Williams, H. and Wischke, M. (2004). Zwischen Widerstandsrecht und starkem Staat. Ein Beitrag zur deutschen Rezeptionsgeschichte von Hobbes. In K. Graf Ballestrem, V. Gerhardt, H. Ottmann and M. Thompson, Politisches Denken. Jahrbuch 2004 (pp.25-42). Berlin: Duncker & Humblot.