Transient expression of rabies virus G-glycoprotein using BHK-21 cells cultured in suspension

Objective To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension. Results A multilevel factorial design was used to quantify effects of temperature (33–37 C), fresh medium addition after the viral adsorption step (100–200 % with respect to the initial cell suspension volume before infection) and harvest time (8–40 h) on RVGP production. Experimental runs were performed in 24-well cell culture plates at a multiplicity of infection (MOI) of 16. An additional experiment in spinner-flask was performed at MOI of 9, using the optimal conditions determined in cell culture plates. Values for temperature, fresh medium addition and harvest time of 33 C, 100 % and 16 h, respectively, ensured the optimal RVGP production in culture plates. The volumetric yield (239 ng ml-1 ) in these conditions was higher than that reported previously for adherent cell culture. In spinner-flasks, the volumetric yield was improved (559 ng ml-1 ). Conclusion These results establish the basis for designing bioprocess to produce RVGP.

MicroRNA 21 is a homeostatic regulator of macrophage polarization and prevents prostaglandin E-2-mediated M2 generation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Agenda 21 local e sustentabilidade socioambiental urbana de Limeira/SP

Pós-graduação em Geografia - IGCE

Análises celulares e moleculares dos efeitos do peptídeo vasoativo angiotensina-(1-7) no processo tumoral pulmonar e a atuação do microrna 21-5P

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Unesp Informa, 2011, ano 2, número 21

Boletim elaborado pela Assessoria de Comunicação e Imprensa da Reitoria da UNESP

Unesp Ciência, 2011, ano 2, número 21

Revista elaborada pela Assessoria de Comunicação e Imprensa da Reitoria da UNESP

Margaret Mae Bryant Papers - Accession 21

This collection consists of Dr. Bryant’s professional and organizational files, biographical data, correspondence, and speeches. Most of the material relates to her publishing efforts, her work as a faculty member at Brooklyn College, and her involvement with professional organizations, especially the New York branch of the American Association of University Women. Most of the material extends form 1950-1975. A list of the more prominent individuals who corresponded with Margaret Bryant has been included as an appendix to the inventory. (For more extensive and comprehensive list of correspondents, see the list included in the collection control file.)

Thomas Belue Collection - Accession 35 - M11 (21)

The Thomas Belue Collection consists of the diary of Thomas Belue of Union County, South Carolina. He enrolled as a private in the Confederate Army in Co. F, Captain C.W. Boyd’s , 15th SCV. The diary covers August, 1861 to September, 1863, and May 1864. Belue describes battles fought in South Carolina, Georgia,Tennessee, and Virginia, mileage covered, camp life and events that occurred during his time in the army. The collection also includes biographical information, genealogical information, a partial transcript of the diary, and copies of Belue’s military records. In addition there is a tintype in a case of Belue in his uniform, two copies of the tintype, and photographs of his gravestone at Gilead Baptist Church Cemetery in Union County, South Carolina.