Retention divergence of terpenes with porous graphitized carbon and C18 stationary phases

The significant divergence between the retention of 16 terpene standards on porous graphitized carbon (PGC) and C18 packing materials are illustrated. The PGC surface is shown to provide a selectivity toward shape, polarity, and structure that is not afforded by the C18 surface. This observation is illustrated by plots of the retention factors similar to those typically used to represent 2D-HPLC separations. A geometric approach to factor analysis was used to measure the separation divergence together with the selectivity and the product selectivity factors of closely related species. When a methanol mobile phase was used with the PGC surface, a large fraction of the separation space could be utilized. That is further reflected by a spreading angle of 80.3°. The PGC material was also successful at resolving structural isomers where the C18 phase was not. It was also found that the choice of the mobile phase is important when using this material. A much larger degree of space utilization was seen with methanol than with acetonitrile that displayed a spreading angle of only 40.8°.

Analysing building energy use using sub metering and external weather data

The Intergovernmental Panel on Climate Change and the McKinsey Greenhouse Gas abatement studies have highlighted reduction of building energy consumption as a primary cost-effective element in the abatement of Global Warming. Nevertheless, the energy investigation in most of our existing building stock remains at a novice level at best. Building sub-metering, by which we mean any secondary, hourly, metering (after the main) of various circuits, provides substantial information on when and where energy is used in specific buildings. Furthermore, combining this information with external weather data provides information beyond basic metering results. This paper discusses three case studies and explains how sub-metering, augmented by external solar and temperature data, benefits energy management and identified problems. It explains how different methods of analysing energy usage allowed: justifiable sizing of a solar photovoltaic system, with a calculated Cooling Degree Unit, identified the absence of savings from a proprietary chiller controller, and the energy variation due to user schedules and external conditions indicated anomalies in energy use. The advantages of wireless access are noted. Extracting information in graphical formats suggests better strategies to understand and control energy use.

Exploring the fantastic: new critical frameworks in an evolving genre

This thesis (creative practice/exegesis) argues that fantasy fiction can function as a progressive socio-political literature. Using the Marxist theory of the Frankfurt School it explores the relationship between fantasy and science fiction via utopian/dystopian representation. Through this dialogue a new understanding of the genre is voiced.

Pauline Kael and the Western genre as critical displacement of self & nation: metaphorics and affects of 'taste' in American film criticism

Pauline Kael (1919–2001) is one of the most influential American film critics of the second half of the twentieth century. Many people are writing on her presently, with at least half an eye to her future cultural, political and historical importance. Certainly the full impact of Kael’s work, both within and beyond the borders of cinema (however defined), has not yet been established. This article unpacks the mechanisms and operations of ‘taste’ in Kael’s writings by using two notions drawn from Roland Barthes’ observations about another key figure of current cultural critique: Julia Kristeva. The comparison of Kael with Kristeva is not dwelt upon; instead, the article focuses on how Kael used the concepts of ‘taste’ and ‘dis-taste’ to draw her readership into a field of what might be termed ‘permanent dissent’. This article concludes by sketching out why Jewish-American Kael’s taste might endure, through the dual transition she occupies from a Cold War to a post-Cold War period, and from an era when cinema was the supreme, undisputed, screen artform, to the rise of the myriad screen technologies of the networked, Internet age.

Sub-lethal UV-C radiation induces callose, hydrogen peroxide and defence-related gene expression in Arabidopsis thaliana

Exposure of plants to UV-C irradiation induces gene expression and cellular responses that are commonly associated with wounding and pathogen defence, and in some cases can lead to increased resistance against pathogen infection. We examined, at a physiological, molecular and biochemical level, the effects of and responses to, sub-lethal UV-C exposure on Arabidopsis plants when irradiated with increasing dosages of UV-C radiation. Following UV-C exposure plants had reduced leaf areas over time, with the severity of reduction increasing with dosage. Severe morphological changes that included leaf glazing, bronzing and curling were found to occur in plants treated with the 1000 J·m(-2) dosage. Extensive damage to the mesophyll was observed, and cell death occurred in both a dosage- and time-dependent manner. Analysis of H2 O2 activity and the pathogen defence marker genes PR1 and PDF1.2 demonstrated induction of these defence-related responses at each UV-C dosage tested. Interestingly, in response to UV-C irradiation the production of callose (β-1,3-glucan) was identified at all dosages examined. Together, these results show plant responses to UV-C irradiation at much lower doses than have previously been reported, and that there is potential for the use of UV-C as an inducer of plant defence.