EPI Update, January 16, 2015

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health.

Quick Reads, February 16, 2015

IDPH Quick Reads is an electronic newsletter produced by the Director’s Office at the Iowa Department of Public Health. IDPH Quick Reads are published every three to four weeks.

Differential protein labeling with thiol-reactive infrared DY-680 and DY-780 maleimides and analysis by two-dimensional gel electrophoresis.

Differential protein labeling with 2-DE separation is an effective method for distinguishing differences in the protein composition of two or more protein samples. Here, we report on a sensitive infrared-based labeling procedure, adding a novel tool to the many labeling possibilities. Defined amounts of newborn and adult mouse brain proteins and tubulin were exposed to maleimide-conjugated infrared dyes DY-680 and DY-780 followed by 1- and 2-DE. The procedure allows amounts of less than 5 microg of cysteine-labeled protein mixtures to be detected (together with unlabeled proteins) in a single 2-DE step with an LOD of individual proteins in the femtogram range; however, co-migration of unlabeled proteins and subsequent general protein stains are necessary for a precise comparison. Nevertheless, the most abundant thiol-labeled proteins, such as tubulin, were identified by MS, with cysteine-containing peptides influencing the accuracy of the identification score. Unfortunately, some infrared-labeled proteins were no longer detectable by Western blots. In conclusion, differential thiol labeling with infrared dyes provides an additional tool for detection of low-abundant cysteine-containing proteins and for rapid identification of differences in the protein composition of two sets of protein samples.

Water Summary Update, January 16, 2015, No. 47

A snapshot of water resource trends prepared by the Iowa DNR in collaboration with the Iowa Department of Agriculture and Land Stewardship, the U.S. Geological Survey, and The Iowa Homeland Security and Emergency Management Department.

Lettre de M. l'abbé de ***, à un curé de ses amis, à l'occasion du serment civique décrété le 16 novembre dernier ([Reprod.])

Collection : Les archives de la Révolution française ; 8.658

Réponse à un chanoine sur le serment civique du 16 novembre 1791 ([Reprod.])

Collection : Les archives de la Révolution française ; 8.661

Apologie du veto opposé par le Roi, au décret des 16 et 29 novembre 1791, sur le serment exigé des prêtres ([Reprod.])

Collection : Les archives de la Révolution française ; 8.671

Iowa Animal Industry News, 2015, Vol. 16, no. 1

Newsletter produced by Department of Agriculture and Land Stewardship about the animal industry in Iowa. Previously titled Animal Industry News.

Développement du second serment appellé civique : décrété le 16 et 29 novembre 1791 ([Reprod.]) / [Barruel]

Collection : Les archives de la Révolution française ; 8.662

Lettre de M. Guiraudez de S. Mézard, archiprêtre de Lavardens, député d'Auch, à ses paroissiens : [au sujet de la Constitution civile du clergé, 16 février 1791] ([Reprod.])

Collection : Les archives de la Révolution française ; 8.538

Epître à M. Lamourette, se disant évêque de Rhône-et-Loire, métropolitain du sud-est sur son instruction pastorale et doctrinale du 16 juillet 1791 ([Reprod.]) / [par M. A. Guillon]

Collection : Les archives de la Révolution française ; 8.1417

Neither Fair Nor Impartial: An Investigation into the Iowa Department of Corrections’ Sanctions Against an Inmate, February 16, 2015

An Investigation into the Iowa Department of Corrections’ Sanctions Against an Inmate

Aging Watch, March 16, 2015, Vol. 15, no. 5

Aging Watch is provided in the spirit of information and education. The opinions expressed by the contributors do not necessarily reflect those of the Department or its programs. The Department shall not be liable for any damages that may result from errors or omissions in information distributed in this publication. Aging Watch will be published regularly during the legislative session and monthly in the interim by the Iowa Department on Aging. “Aging Watch.” The Department is providing this update to better inform you about policy affecting older Iowans. In addition to policy updates from the statehouse and the nation’s capitol, you’ll learn about Department programs and changes affecting the landscape. As you’ll learn reading this and future editions, big changes are coming for the Iowa Aging Network. Over the next year the Department will be reducing the number of local Area Agencies on Aging, as required by legislative action. Not surprisingly, this is a major change for everyone.