Influência da fonte alimentar na infecção de triatomíneos por Trypanosoma cruzi

Embora a doença de Chagas seja amplamente estudada, ainda não se conhece todos os interferentes que modulam a relação parasitária Trypanosoma cruzi- vetor- hospedeiro vertebrado. Sabe-se que as aves são refratárias ao T. cruzi, ou seja, o parasito não consegue estabelecer parasitismo nas mesmas, enquanto que nos camundongos a infecção ocorre. Essa refratariedade representa um exemplo de resistência natural do hospedeiro a uma infecção parasitária. Originalmente, acreditava-se que isso ocorria devido à presença de um anticorpo natural contra o T. cruzi no sangue das aves, porém estudos atuais atribuem tal atividade ao sistema complemento presente no sangue das mesmas. Pretendeu-se avaliar neste projeto se a referida característica peculiar do sangue das aves interfere no processo e no nível de infecção dos triatomíneos, em especial de Rhodnius neglectus, espécie de comprovada importância epidemiológica. Para tanto, utilizou-se a cepa Y de T. cruzi e ninfas de 4º estádio de R. neglectus. Avaliou-se também a capacidade lítica do soro de galinha em formas epimastigotas e tripomastigotas em meio de cultura LIT, utilizando a cepa Y. Referente à influência de diferentes fontes alimentares na infecção de R. neglectus por T. cruzi, observou-se um decréscimo no número de exemplares infectados após alimentação em patos , apresentando Índice de Infecção médio de 19,74%. Por outro lado, exemplares infectados após alimentação em camundongos não apresentaram diferença significante na parasitemia total. Os testes in vitro demonstraram que a adição de soro de galinha em meio de cultura LIT, com formas epimastigotas e tripomastigotas, foi capaz de diminuir notoriamente a contagem de parasitos móveis até a contagem zero, uma hora após a adição. O conjunto desses resultados sugere que a refratariedade das aves ao T. cruzi influencia a infecção de R. neglectus pelo flagelado e foi demonstrada, in vitro, pela ...

Reprodutibilidade das respostas metabólicas e cardiorrespiratórias fisiológicas em um protocolo de exercício intermitente realizado com recuperação passiva e ativa

The main objective of this study was to analyze the reliability of blood lactate concentration ([La]), oxygen uptake (VO2) and heart rate (FC) in an intermittent protoco, performed at 95%VO2max with passive or active recovery in untrained subjects. Participated of this study, active healthy males with 20 to 25 years, which were doing aerobic exercises witha weekly frequency of 3 sessions at least. The individulas performed, in different days, the following protocols in a cyclergometer: 1) An incremental test until exhaustion to determine maximal oxygen uptake (VO2max) and the intensity at VO2max; b) Two transitions at 95%VO2max for the determination of the VO2 kinetics parameters and; c) Two intermittent tests until exhaustion, with repetitions at 95% IVO2max and with durantion defined as being half of the duration of the slow component. The duration of the recovery was half of the duration of the effort (effort:pause of 2:1). This test was performed with passive (GP) and active recovery (GA). The VO2 and FC were measured continulously in both tests. Blood collections were performed for the determination of the [La]. There was significant correlação in both groups for VO2 (ATIVA - 0.94, PASSIVA - 0.75), [La] (ATIVA - 0.83, PASSIVA - 0.90) and FC (0.93) only for the passive group. Thus, it can be concluded that the cardiorrespiratory and metabolic responses present good realiability in an intermittent exercise with active or passive recovery

Diabetes mellitus e esteatose hepática em ratos: efeitos de um protocolo de treinamento aeróbio

The present study aimed to analyze the effects of exercise performed at aerobic/anaerobic transition on non-alcoholic hepatic steatosis (NAHS) markers in diabetic rats. Adult (60 days) male Wistar rats were divided into 4 groups: sedentary control (SC), trained control (TC), sedentary diabetic (i.v. alloxan injection) (SD) and trained diabetic (TD). At the beginning of the experiment, all the animals were submitted to maximal lactate steady state test (MLSS) in order to identify the aerobic/anaerobic metabolic transition during swimming exercise. The trained groups were submitted to swimming, supporting overloads (% of body weight – b.w.) equivalent to MLSS intensity, 1h/day, 5 days/week, during 8 weeks. We analyzed: serum ALT, AST, albumin, glucose and free fat acids (FFA), body weight and total lipid concentrations in the liver. The diabetic groups showed higher (ANOVA two-way, p<0.05) serum glucose (SD=200% and TD= 150%) and weight loss (SD= 15.0% and TD= 8.5%) compared to controls and the SD showed higher glucose concentration and weight loss when compared to TD. The work load (% b.w.) equivalent to the MLSS was lower in TD (4.7%) than in TC (5.6%) group. The NAHS markers (U/L) did not show... (Complete abstract click electronic access below)

Utilização do protocolo anycast em ambientes virtuais distribuídos visando à escalabilidade do sistema

Distributed Virtual Environments (DVE) allow multiple users interact with a virtual world by sharing, manipulating objects. Each user has his/her own vision of this world and every changes of state of the environment are distributed among other users. Due to technological constraints, most systems for distributed virtual environments support a limited number of users. The major limitation for development of DVE scale are imposed by the communication system. As the number of users grows, the amount of bandwidth required to exchange information in real time among stations to upgrade the environment and keep it in a consistent state for all connected users. Based on this situation is proposed a framework that uses the protocol anycast in the application layer that provides a load balancing among servers in the region and aims at reducing the latency of message delivery between participants selecting the DVE server best suited for the region participant to connect to the environment

Análise de variáveis eletromiográficas e cinemáticas em protocolo incremental de corrida na esteira

Muscle fatigue is described as a cause of injuries among the many related to the running practice. Therefore, the purpose of this study was to analyze the behavior of the amplitude (RMS) and median frequency (MF) of EMG signal of the iliocostalis (CI), rectus femoris (RF), vastus lateralis (VL), vastus medialis (VM) biceps femoris (long head) (BFCL), tibialis anterior (TA) and gastrocnemius (lateral) (LNG) from the right lower limb, and the behavior of the parameters of amplitude (AP) and frequency (PF) spent in different percentages of the maximum speed during incremental protocol of treadmill running. 10 volunteers participated in this study, athletes, male, aged between 18 and 30 years with no history of injury in lower limbs and similar anthropometry. The protocol consisted of a treadmill test with initial velocity of 10 km.h-¹ and increments of 1 km.h-¹ each three minutes until volitional exhaustion, without rest interval. Synchronized collections were made of electromyographic and kinematic data. The signals were obtained through an acquisition module of biological signals (Telemyo 900 - Noraxon - USA) and software (Myoresearch - Noraxon - USA) calibrated with a sampling frequency of 1000 Hz, gain 2000 times. The raw data were filtered with a 60Hz notch filter, high pass and low pass 20Hz to 500Hz. To capture the image was used a digital video recorder (model NV-GS320, PANASONIC brand), and for image scanning and kinematic data collection was used the software Peak Motus 9.0 (ViconPeak). To obtain the values of RMS and FM analyzed the last ten passes of each speed through a specific routine (Matlab). To obtain these variables AP (m) and FP (stride I min) were analyzed for the last ten past each speed, using specific software (Peak Motus 9.0). After verification of data normality (Shapiro-Wilk) and homogeneity of the data (Levene), the comparison ...(Complete abstract click electronic access below)

Validação dos parâmetros aeróbio e anaeróbio a partir de protocolo não invasivo aplicado ao exercício resistido - supino reto

The purpose of this study was to investigate the validity of critical force test from maximal lactate steady state (MLSS) during resistance test using straight bench press. Five healthy male volunteers aged (22.6 ± 2.88 years), weight (76.3 ± 11.49 kg) e height (182.6 ± 7.54cm), trained in resistance exercise, and performed four diferent test to determine: one maximal effort (1RM), critical force using the critical power model (force vs 1/time limit - 20, 25 and 30% 1RM). The CF was the linear coefficient and the anaerobic impulse capacity (CIA) was the angular. MLSS was determined using loads of 80, 90, 100 and 110% of critical force. Blood lactate samples were abtained at each 300sec between each stage of total 1200sec. Maximal 30s test (M30) was accomplished with load of 25% of body weight in SBP. The results showed that the 1 RM was 79.4 Kgf (± 16.98), CF 10.1N (± 2.25), CIA 1756.82 N.s (± 546.96) and the R² 0.984 (± 0,02). The MLSS occurs at 100% CF load. The lactate concentration at the MLSS was 2.2 mmol/L (± 0.77). Significant correlation was observed between MLSS and CF on SBP (r = 0.88 p = 0.05). In M30 the minimum, mean and peak power were (25.0 ± 4.9, 28.0 ± 4.9, and 30.0 ± 4.6 kgf.rps, respectively). The fatigue index was 18.0% (± 6,8). The M30 was significantly correlated with Ppeak and Pmean (r = 0.98 for both, p = 0.003). The CF means has been validated to predict the resistance training and the CIA show to be a representative anaerobic parameter in straight bench press.

Significados da reforma agrária em bairros periféricos de Presidente Prudente (SP): uma tentativa de diálogo entre a luta pela Terra e a soberania alimentar

La lucha por la tierra en el Brasil se confunde con la própria historia del país, erigida sobre una cuestión agraria turbulenta en la cual ha predominado la hegemonía de los grandes proprietarios de tierra, tanto en la forma de latifundistas, cuanto, más recientemente, de los empresarios del agribusiness, caracterizando una versión más moderna de la dominación de la lógica del capital en detrimento de las demás formas de organización de la vida. A partir de la segunda mitad del siglo XX, ocurre un aguzamiento de los conflictos por la tierra con la emergencia de los principales movimientos sociales en Brasil, como el MST. Esos movimientos surgen en un contexto de transformación de la formación socioespacial brasileña, marcada por la creciente urbanización del país, lo que repercute em las sus formas de organización, pasando a comparecer una preocupación con la población urbana en las discusiones y acciones en pro de la Reforma Agraria. Yuxtaponiendo-se a eso proceso, también ha emergido fortemiente en las últimas décadas la problemática de la hambre en el mundo, tanto a través de los dados alarmantes de los organismos supranacionales (FAO), como del debate impulsado por los movimientos sociales acerca de la Soberanía Alimentaria (Via Campesina). Todos esos elementos merecen atención em esto trabajo, que busca aprehender cómo se efectiva la participación de barrios de la periferia pobre de la ciudad de Presidente Prudente/SP en el proceso de lucha por la tierra en el Pontal del Paranapanema, haciendo eso por lo medio de una lectura geográfica de esa realidad que prime por la superación analítica de la disociación entre ciudad y campo, como se fueran espacios inertes entre sí. Además, es nuestro objetivo destacar las motivaciones que llevan los sujetos protagonistas de eso proceso a dejaren la ciudad y adhieriren a la lucha por la tierra, permitiendo identificar elementos que extrapolan la esfera económico-material

Proposta de arquitetura e protocolo para a geração automática de assinaturas de malwares

Given the exponential growth in the spread of the virus world wide web (Internet) and its increasing complexity, it is necessary to adopt more complex systems for the extraction of malware finger-prints (malware fingerprints - malicious software; is the name given to extracting unique information leading to identification of the virus, equivalent to humans, the fingerprint). The architecture and protocol proposed here aim to achieve more efficient fingerprints, using techniques that make a single fingerprint enough to compromise an entire group of viruses. This efficiency is given by the use of a hybrid approach of extracting fingerprints, taking into account the analysis of the code and the behavior of the sample, so called viruses. The main targets of this proposed system are Polymorphics and Metamorphics Malwares, given the difficulty in creating fingerprints that identify an entire family from these viruses. This difficulty is created by the use of techniques that have as their main objective compromise analysis by experts. The parameters chosen for the behavioral analysis are: File System; Records Windows; RAM Dump and API calls. As for the analysis of the code, the objective is to create, in binary virus, divisions in blocks, where it is possible to extract hashes. This technique considers the instruction there and its neighborhood, characterized as being accurate. In short, with this information is intended to predict and draw a profile of action of the virus and then create a fingerprint based on the degree of kinship between them (threshold), whose goal is to increase the ability to detect viruses that do not make part of the same family

O ato alimentar em três espécies de Artibeus de grande porte (Chiroptera: Phyllostomidae) do Sudeste brasileiro

The alimentary act of three species of frugivorous bats of the genus Artibeus: A. lituratus, A. fimbriatus and A. planirostris, in a total of nine individuals, was studied by observations in field and captivity, during the months of May to September of 2008. With the objective to caracterize the form of consumption of two tipes of fruits (soft and consistent) and it relates with the dentary structure of this bats, the alimentary act was film and analysed later. It had little variation between the species. The fruits had been consumed in similar way, being that statistics analisis had demonstrated that consistent fruits had received more lateral bytes than the soft fruits. The dentary analisis shown that the posterior teeth (pre-molar and molar) present important characteristcs to triturate pieces of more consistent fruits and the incisors serve to scrape the pulp of the soft fruits. This study it served to describe the form of feeding of frugivorous bats, that little is explained in the studies of alimentary behavior

Alimentar ou ser alimentado?: a expansão da agroindústria canavieira e a soberania alimentar em Flórida Paulista/SP

Devido às características de uso e ocupação do território nas últimas duas décadas, marcadas pela substituição da heterogeneidade da paisagem camponesa pela monotonia dos mares verdes do agronegócio, o município de Flórida Paulista contempla elementos representativos para analisarmos algumas das resultantes entre a expansão da cana-de-açúcar e a produção de alimentos voltados ao abastecimento dos locais próximos. Com isso buscamos entender, com base no recorte eleito para estudo, o percurso do alimento no espaço, desde as pequenas propriedades camponesas que ainda resistem à imposição do formato único, até os diversos pontos de venda/aquisição de alimentos disponíveis na área urbana, além do CEASA de Presidente Prudente/SP, responsável pela distribuição da maior parte dos produtos alimentícios encontrados nas bancas do município. A expansão das áreas monocultoras encontra-se sintonizada aos imperativos da lógica do abastecimento alimentar referenciado no movimento do alimento no espaço, no qual áreas antes policultoras, produtoras de alimentos, são sobrepostas pela geometria monocultural dos canaviais, o que diminui as possibilidades de abastecimento local e reforça o discurso de que os alimentos devam ser garantidos a partir de sujeitos estranhos ao lugar. Alimentar ou ser alimentado, soberania ou segurança, poder de decisão que emana autonomia ou dependência, constituem o eixo central no debate aqui proposto, de modo a identificar, a partir do movimento que denuncia a lógica por detrás do alimento, atores e setores entre a terra e o alimento. Com o objetivo de compreender as implicações do modus operandi próprio ao agronegócio... (Resumo completo, clicar acesso eletrônico abaixo)

Efeitos de diferentes fontes de gordura alimentar na resposta a aspiração folicular de cabras anglo-nubianas

Pós-graduação em Medicina Veterinária - FCAV

Prostaglandina E2 em cães portadores de linfoma multicêntrico submetidos ao protocolo quimioterápico de Madison-Wisconsin associado ou não a um inibidor de COX-2

Pós-graduação em Medicina Veterinária - FCAV

Quantificação imunofenotípica de células T reguladoras em cães acometidos por linfoma multicêntrico submetidos ao protocolo quimioterápico chop associado ou não a um firocoxib

Pós-graduação em Medicina Veterinária - FCAV

Estado nutricional de pré-escolares e consumo alimentar de suas famílias no Município de Ponta Grossa-Pr: a influência das condições socioeconômicas e das políticas públicas

Pós-graduação em Alimentos e Nutrição - FCFAR

Aproveitamento de sementes de abóboras (Cucurbita sp) como fonte alimentar

Brazil processes much of its fruit by producing natural juices, candy preserves, extracts and pulps; however, almost 60% of their total weight derives from peels, leaves and seeds. As a result, the emphasis lies most strongly on the use of waste, especially waste that is not commonly used by the food industry or by consumers. The use of seeds would increase the viable sources of raw materials, lower operating costs of industries and would promote the development of new food products, since they are our main sources of protein, fat, fiber, functional compounds, vitamins and minerals salts. This paper presents alternatives for the use of pumpkin seeds (Cucurbita sp), which are often underutilized or discarded. It also demonstrates the bioactive and anti-nutritional compounds and their health effects.