Qualidade fisiológica de sementes de Moringa oleifera lam. por meio da análise de imagens

Several tests that evaluate the quality of seeds are destructive and require time, which is considered long and expensive in the processes that involves the production and marketing of seed. Thus, techniques that allow reducing the time related to assess the quality of seed lots is very favorable, considering the technical, economic and scientific point of view. The techniques images of seed analyzed both by X-ray such as digital images, represent alternative for this sector, and are considered reproducible and fast, giving greater flexibility and autonomy to the activities of production systems. Summarily, the objective was to analyze the internal morphology of seeds of this species through x-rayed images and the efficiency of weed seed area increased during soaking through image analysis and compare them with the results of germination tests and force the evaluation of physiological seed quality. For X-ray tests, the seeds were exposed for 0.14 seconds at radiation 40kV and 2.0 mAs. Were analyzed images using the ImageJ program and subsequently put to germinate in B.O.D chamber at 27 ° C, in which there was the comparison of results for germination. To determine the test area increase (% IA), seeds were used with and without seed coat, maintained the B.O.D chamber at 15 ° to 20 ° C, the seeds were photographed before and after the soaking period, the results were compared to the germination rates. For the X-ray test, it was observed that seeds with empty area greater than 20%, showed a higher percentage of abnormal seedlings. And the area increment analysis showed that it is possible to rank the batch after 8 hours of imbibition at 15 ° C according to the germination and vigor tests

Coordenação dos sistemas antioxidantes da catalase e da ascorbato peroxidase durante o envelhecimento acelerado de sementes de cártamo e girassol

World consumption of vegetable oils has increased in recent years because of its application in food, chemical, pharmaceutical and, more recently, energy industry. However, oilseeds, which these oils are extracted, have low viability, affecting the cultivation and productivity of these species. The aim of this study was to analyze the effect of aging on the coordination of catalase (CAT) and ascorbate peroxidase (APX) antioxidant systems in safflower and sunflower. . Therefore, seeds were subjected to accelerated aging for 3, 6 and 9 days and grown in moistened paper towel for 72 hours. Additionally, before accelerated aging, sunflower seeds were pretreated by osmopriming with 10 mM ascorbate (ASC) or 3 amino 1,2,4 triazol (3-AT), a specific inhibitor of CAT activitie. The method of artificial aging used was efficient in both species, because it caused a decrease in germination, seedling development and growth, especially in safflower. The aging caused inhibition of CAT activity for both species and to compensate for such inhibition , sunflower increased mRNA expression of this enzyme , while safflower mobilized over the activity of APX. Analysis of the expression of malate synthase and sugar content demonstrated that sunflower seeds consumes lipid reserves in quiescent state, while the safflower is more dependent on carbohydrate. Pretreatment with 3-AT inhibited CAT activity and stimulated the APX, though with ASC acted reverse on these systems. None of the treatments recovered the physiological decline aging. It is concluded that aging change the oilseeds antioxidant metabolism, despite interspecies variations in response to this process, the depletion of the CAT antioxidant system was common. Because of this we propose that the measurement of CAT activity can be used to identify aging seed lots.

Construção de bibliotecas de cDNAs subtraídos a partir das sementes de Mamona submetidas à estresse hídrico e análise in silico de transcritos potencialmente expressos sob condições de estresse: Metalotioneina, proteína reprimida por auxina de 12.5 kDa e Glutelina tipo-A3

Estresses ambientais abióticos são fatores que causam respostas ao nível molecular, fisiológico e morfológico em plantas, dependendo também de sua intensidade e duração. É visto que algumas espécies apresentam tolerância a condições estressantes e ao mesmo tempo são fontes naturais de matéria prima para indústria. Nesse contexto encontra-se a mamona (Ricinus comunnis L.), principal fonte de óleo de rícino valorizado por suas aplicações farmacêuticas e principalmente industriais, vem sendo usada como cultura em regiões onde a disponibilidade de água é reduzida, usada como fonte de renda para agricultura da região nordeste brasileira. Visto que pouco se sabe sobre as respostas moleculares que levam essa planta a tolerar regiões secas e como as sementes, principais foco de interesse, respondem a essa escassez, nesse trabalho foram construídas duas bibliotecas de cDNAs, onde a partir de uma abordagem subtrativa, continham RNAs diferencialmente expressos em sementes de plantas mamona submetidas ao estresse hídrico durante 5 dias (biblioteca L7), e a outra RNAs diferencialmente expressos em sementes controle (biblioteca L5). A biblioteca L7 apresentou a maior variedade de transcritos com um total de 182. A maior parte das funções estabelecidas pelo sistema Gene Ontology - GO, foram direcionadas aos “Processos Metabólicos” (526), em segundo “Respostas a estímulos” (57), o terceiro termo mais abundante foram referentes a “Desenvolvimento”(26). Já na biblioteca L5, foram encontrados 91 transcritos, com maior parte de suas funções referentes a “Processos Metabólicos”(413), em segundo “Respostas a estímulos” (8) e em terceiro Regulação (6). Alguns dos transcritos da biblioteca L7 foram escolhidos para análise por repetirem-se mais de 3x e não aparecerem na biblioteca L5, o que indica uma possível regulação positiva sobre estresse. As análises sobre Metalotioneína (4x), mostraram que a sequência de proteica apresentava os domínios conservados que a caracterizava como tipo II, onde são encontrados dois domínios funcionais ricos em cisteína com posições altamente conservadas, desempenhando a função de ligar-se a metais pesados, correlacionadas assim como a atividade de eliminação EROs e defesa contra o estresse oxidativo, além de apresentar homologia com a sequência de Bruguiera gymnorhiza, uma planta de mangue adaptada a ambientes salinos. Analisamos também os transcritos da referente a proteína AUXIN-REPRESSED 12.5 KDA (3x), apontada como sendo reprimida pelo hormônio auxina e associada ao processo de dormência da semente, é descrito em uma família gênica onde vários membros pertencem as vias de resposta ao estresse. Por último, analisamos a proteína GLUTELIN TYPE-A 3 (5x), uma importante proteína de armazenamento com caráter hidrofílico, possivelmente direcionada para o vacúolo. Em nosso trabalho foi possível observar um aumento de transcritos em relação a subtração controle, possivelmente reflexo do aumento do metabolismo da semente, tanto para resposta defensiva ao estresse hídrico quanto para o amadurecimento rápido da semente onde foram observados transcritos referentes a resposta oxidativa, controle hormonal, proteínas de reserva e produção de óleo.

Testes para a avaliação da qualidade fisiológica de sementes de Moringa oleifera Lam

The evaluation of seed vigor is an important factor for detection of lots of high quality seeds, so that development of procedures to evaluate the physiological potential has been an important tool in quality control programs seeds. In this sense the study aimed to adapt the methodologies of accelerated aging, electrical conductivity and potassium leaching to evaluate Moringa oleifera seed vigor LAM.. Therefore, four lots of moringa seeds were subjected to the germination tests, seedling emergence, speed of emergence index, emergence first count, length and dry mass of seedlings and cold test for their physiological characterization, in addition to accelerated aging, electrical conductivity and potassium leaching. The experimental design was completely randomized with four replications of 50 seeds and the means compared by Tukey test at 5% probability. For accelerated aging the periods were studied aging 12, 24 and 72 hours at 40, 42 and 45°C. For the electrical conductivity test was used to a temperature of 25°C for periods of 4, 8, 12, 16 and 24 hours of immersion in 75 to 125 mL of distilled water, using 25 to 50 seeds, and for potassium leaching test samples were used 25 to 50 seeds, placed in plastic cups containing 70 and 100 mL of distilled water at 25°C for periods of 1, 2, 3, 4, 5 and 6 hours. From the results obtained, it can be inferred that the methods best fit for the accelerated aging test Moringa seeds were a temperature of 40°C for 12 to 72 hours, 42°C 72 hours 45°C 24 hours . In the electrical conductivity test Moringa seeds, the combination of 50 seeds in 75 mL distilled water for a period of immersion of 4 hours and 50 seeds in 125 mL of 4 hours were efficient for the differentiation of lots of Moringa seeds as to vigor and for potassium leaching test moringa seeds, the combination of 50 seeds in 100mL of distilled water allowed the separation of lots of four levels of vigor, at 2 hours of immersion, showing promise in evaluate the quality of moringa seeds.

Testes para a avaliação da qualidade fisiológica de sementes de Moringa oleifera Lam

The evaluation of seed vigor is an important factor for detection of lots of high quality seeds, so that development of procedures to evaluate the physiological potential has been an important tool in quality control programs seeds. In this sense the study aimed to adapt the methodologies of accelerated aging, electrical conductivity and potassium leaching to evaluate Moringa oleifera seed vigor LAM.. Therefore, four lots of moringa seeds were subjected to the germination tests, seedling emergence, speed of emergence index, emergence first count, length and dry mass of seedlings and cold test for their physiological characterization, in addition to accelerated aging, electrical conductivity and potassium leaching. The experimental design was completely randomized with four replications of 50 seeds and the means compared by Tukey test at 5% probability. For accelerated aging the periods were studied aging 12, 24 and 72 hours at 40, 42 and 45°C. For the electrical conductivity test was used to a temperature of 25°C for periods of 4, 8, 12, 16 and 24 hours of immersion in 75 to 125 mL of distilled water, using 25 to 50 seeds, and for potassium leaching test samples were used 25 to 50 seeds, placed in plastic cups containing 70 and 100 mL of distilled water at 25°C for periods of 1, 2, 3, 4, 5 and 6 hours. From the results obtained, it can be inferred that the methods best fit for the accelerated aging test Moringa seeds were a temperature of 40°C for 12 to 72 hours, 42°C 72 hours 45°C 24 hours . In the electrical conductivity test Moringa seeds, the combination of 50 seeds in 75 mL distilled water for a period of immersion of 4 hours and 50 seeds in 125 mL of 4 hours were efficient for the differentiation of lots of Moringa seeds as to vigor and for potassium leaching test moringa seeds, the combination of 50 seeds in 100mL of distilled water allowed the separation of lots of four levels of vigor, at 2 hours of immersion, showing promise in evaluate the quality of moringa seeds.

Biometria de frutos e sementes, crescimento e produção de mudas de Campomanesia adamantium Blume e Campomanesia pubescens O. Berg

Campomanesia adamantium and Campomanesia pubescens are morphologically similar, occur in the same regions of the Cerrado, are difficult to differentiate and exhibit naturally slow growth and development. The objectives of this study were to analyze fruit and seed biometric data, emergence capacity and seedling growth characteristics. Fruit from both species was collected and used to measure biometric data of the fruit and seeds and to set up two emergence and two seedling growth experiments. C. adamantium fruit is round and has wider, pale yellow seeds while C. pubescens fruit is ellipsoidal to pyriform with yellow gold seeds. C. adamantium’s greater fresh fruit mass and biometric variability favors selection of promising material for commercialization. Seed drying reduced the speed and rate of seedling emergence of C. adamantium, but had no effect on C. pubescens. Leaf number, height, shoot dry weight and the ratio of shoot dry weight to root dry weight were greater in C. adamantium than in the slower growing C. pubescens. Increases in substrate volume favor seedling development. Slow-release fertilizer application at 1g per 115 cm3 substrate increased leaf formation, plant height and shoot dry weight of seedlings of both species and lateral budding in C. adamantium. Until 120 days after transplant, lateral budding was not observed in C. pubescens seedlings. For all traits evaluated in this experiment, C. adamantium seedling growth was greater than that of C. pubescens.

Tratamento Endovascular de Patologia da Aorta Torácica: Experiência Institucional

Introdução: O tratamento endovascular da aorta torácica (TEVAR) é uma modalidade terapêutica emergente que tem vindo a revolucionar a abordagem de diferentes tipos de patologia da aorta na sua localização torácica. Objetivos: Avaliação da experiência institucional do serviço de angiologia e cirurgia vascular. Métodos: Análise retrospetiva da série consecutiva de todos os doentes com patologia da aorta torácica e/ou toracoabdominal submetidos a TEVAR na nossa instituição. Foram excluídos aqueles com uso concomitante de endopróteses fenestradas/ramificadas abdominais. Resultados: Desde abril de 2005 até abril de 2014, 79 doentes foram submetidos a TEVAR, com idade média de 66 ± 12,83 anos (máx: 86; mín: 14). As indicações incluíram: 46 aneurismas (58%), 17 dissecções aórticas clássicas tipo B (22%), 13 no contexto de outras síndromes aórticas agudas (16%), 2 por ateroembolismo (3%) e um por fístula aortoesofágica (1%). Na patologia aneurismática, a distribuição anatómica da doença foi a seguinte: 5 na aorta ascendente e arco aórtico (11%), 35 na aorta torácica descendente (76%) e 6 toracoabdominal (13%). O diâmetro médio das dilatações aneurimáticas foi de 69,64 mm (máx: 150 mm). A rotura foi uma apresentação da patologia em 21,5% dos doentes (n = 17); 20,9% dos doentes tinham antecedentes de cirurgia aórtica prévia. A dissecção aórtica tipo B complicada foi a segunda indicação mais comum, sendo de apresentação aguda em 13 (76%) e crónica em 4 (24%). As complicações na base da intervenção foram dilatação aneurismática em 35% (n = 6), malperfusão com isquemia de órgão alvo 47% (n = 8), desconhecida em 18% (n = 3). Foi realizada extensão distal com stent descoberto (Petticoat) em 9 casos (41,2%) e foram realizados procedimentos adjuvantes em 18% (stenting renal n = 2; stenting ilíaco n = 1). Dentro das outras síndromes aórticas agudas, o TEVAR foi realizado no contexto de úlcera aórtica penetrante (n = 4), hematoma intramural (n = 4) e os restantes por rotura/pseudoaneurisma (n = 5). As endopróteses utilizadas foram: 32 Valiant Medtronic®, 15 TAG Gore®, 25 Zenith TX2 Cook®, 2 Zenith TX1 Cook®, uma Relay Plus®, 3 Talent Medtronic® e outras em 1%. A mediana de dias de cuidados intensivos foi 2 (intervalo 0-42) e a mediana de suporte tranfusional foi de 2 UCE. A taxa de mortalidade aos 30 dias ou intra-hospital foi de 18% (n = 14). Atendendo ao timing da cirurgia, a taxa de mortalidade aferida nos casos electivos foi de 8% (4/50) e nos urgentes atinge os 35% (10/29). Intraoperatoriamente foram tratadas 7 complicações relacionadas com vaso de acesso membro, 2 casos de dissecção aórtica iatrogénica, um caso de trombose arterial inferior e um endoleak tipo IA. A taxa de reintervenções foi de 17%, com as seguintes indicações: 9 endoleaks, 2 isquemias mesentéricas e 2 fístulas aortoesofágicas. Conclusões: A série apresentada traduz uma experiência institucional favorável com resultados reprodutíveis e que o TEVAR é um procedimento seguro e eficaz para o tratamento de diferentes patologias da aorta torácica, quando comparado com o tratamento cirúrgico aberto.

Potencial fisiológico de sementes de soja enriquecidas com molibdênio no armazenamento e sua influência na atividade de enzimas do metabolismo do nitrogênio

Molybdenum is one of the essential micronutrients for soybeans, acting directly on nitrogen metabolism as enzyme cofactor of nitrogenase. Usually, this nutrient is supplied to the plants through seed treatment or foliar application. The aim of this study was to evaluate the molybdenum effects by foliar in the physiological potential of soybean seeds and verify its interference in the enzyme activities involved in nitrogen metabolism. Soybean seeds of BMX Turbo cultivar were used, produced in Erechim, RS, harvest 2013, from plants treated with the following Mo concentrations: 0; 25; 50 and 75 g ha-1, supplied through two commercial products (Biomol and Molybdate) and stored during 0 and 6 months in uncontrolled conditions. The first experiment was conducted in Seedtes Seed Analysis Laboratory in Pato Branco, PR. The used design was completely randomized in a factorial analysis 4 x 2 x 2 with four replications each. The physiological potential of the seeds was evaluated by the germination test, seedling growth, accelerated aging and emergence on the soil. The second experiment was conducted in a greenhouse, where the seeds derived from treatments with different concentrations of Mo: 0; 25; 50 and 75 g ha-1 supplied through two commercial products (Biomol and Molybdate) were grown in vases. The used design was completely randomized in a factorial analysis 4 x 2 with four replications. Evaluations were performed when the plants reached the R1 phenological stage concerning the nodulation, dry matter of root and shoot of the plants and the determination of the activity of the enzymes glutamine synthetase and glutamate synthetase and the content of total soluble proteins. The data were submitted to variance analysis and when significant they were assessed by Tukey’s test for comparison of products and seed storage and with regression study to the concentrations at 5% probability. Analyses were performed using SISVAR statistical software. The soybean seed storage under uncontrolled conditions affected the seed vigour produced with Mo, regardless of the commercial product used during production. The application of Mo through foliar positively influences the production of soya beans which presented increasing responses in the germination and vigour with the application of Mo above 25 g ha-1 . The enrichment of Mo through foliar did not affect the nodulation of plants of the next generation, however, the use of Mo above 25 g ha-1 provided an increase in the activity of enzymes involved in nitrogen metabolism as well as on the total protein content.

Uso de indicadores bioquímicos na qualidade fisiológica de sementes florestais

The scientific research in seed technology is based on techniques that aim the reduction of costs and time, standardization, improvement and establishment of analytical methods while maintaining a high level of reliability of the results. This study sought to elucidate the reliability of electrical conductivity and pH of the exudate compared to the classic germination test, which was developed in two separate studies, however interrelated with each other, as to their final goals. The experimental material of this study consisted of seeds of the species Aspidosperma parvifolium (guatambu), Aspidosperma polyneuron (peroba-rosa), Cabralea canjerana (canjerana), Cariniana legalis (jequitibá), Gallesia integrifolia (pau-d'alho), Handroanthus chrysotrichus (ipê-amarelo), Lonchocarpus campestris (rabo-de-bugio) and Pterogyne nitens (amendoim-do-campo). The physiological quality of the studied seed species was evaluated through the electrical conductivity and pH test of the exudate by mass and individual methods being compared and correlated with the results obtained in the germination test. In addition to the tested methods, imbibition periods of the seeds were evaluated for conductivity and pH, which corresponded to 2, 4, 6, 8, 24 and 48 hours. The electrical conductivity test was efficient in both of the used methods to evaluate the physiological quality of the studied seed species when compared to the standard germination test. The pH test of the exudate applied by the individual method was more efficient and thorough to evaluate the physiological quality of the studied seed species, than the mass method. For the species Gallesia integrifolia, Cariniana legalis and Lonchocarpus campestris the pH tests of the exudate tests were not efficient due to poor or absent correlation between germination and pH.

Determinación de la eficacia de la profilaxis antibiótica en pacientes con heridas limpias y limpias contaminadas, servicio de cirugía general, Hospital Vicente Corral Moscoso Cuenca-Ecuador 2007: estudio a realizarse en patologia biliar, apendicular y herniaria

Determinar la prevalencia de infección del sitio de la herida quirúrgica y factores asociados en pacientes que acudieron al Centro Quirúrgico del hospital Vicente Corral Moscoso, de la ciudad de Cuenca, en el 2007-2008.Se realizó un estudio transversal; en un universo de 385 pacientes. Los datos se obtuvieron por entrevista directa y se analizaron con el software Excel y Epi-Info. Resultados. La prevalencia de infección del sitio de la herida quirúrgica fue del 10.6(IC 959.03-12.17). La mayoría fueron de sexo femenino; la edad varió entre los 16 y 87 años. La asociación entre infección del sitio de la herida quirúrgica con cirugía de emergencia proporcionó una p=0.019 (IC 95de la RP 1.10 - 3.99); con cirugía convencional p= 0.778 (IC 95de RP 0.61-1.95); con herida limpia p= 0.238 (IC 95de RP 0.10 - 1.55); con la utilización de cefazolina p= 0.023 (IC 95de la RP 0.09-0.94) y con el tiempo quirúrgico menos de una hora p= 0.000 (IC 95de RP 0.01-0.32). Conclusión, la prevalencia de infección del sitio de la herida quirúrgica fue del 10.6(IC 959.03-12.17). La cirugía de emergencia fue factor de riesgo para infección del sitio de la herida quirúrgica y la cefazolina, utilizado como antibiótico profiláctico, y el tiempo quirúrgico, menos de una hora, fueron factores de protección

Indução de resistência e tratamento de sementes de soja com fosfitos de potássio

The use of inputs containing phosphites have been presenting results in many studies, taking on importance to the control of diseases in some cultures and demonstrating the resistance induction in seedlings, with ability to activate defense mechanisms, conferring protection to plants against microorganisms. The soybean crop is recognized for its importance in providing grains and derivatives for human consumption, animal, production of biofuels, pharmaceuticals, among others. Positive results obtained through studies based on resistance inducers in some cultures arouse the interest for further study. The objective of this study was to evaluate the effect of potassium phosphites on the resistance induction and treatment of soybean seeds. Therefore were conducted four laboratory studies at the Federal Technological University of Paraná, Campus of Dois Vizinhos. In the first study it was evaluated the quality attributes of the seeds and the resistance induction as seed treatment. Then it was verified that phosphites have action upon the seedlings metabolism in due to seed treatment, having the phosphite Reforce® contributed to seed quality attributes and phosphites FitofosK® and Fitofos K Plus® induced the resistance increasing the activity of β-1,3-glucanase. In the second study it was evaluated the the resistance induction in soybean cotyledons, in which the phosphites demonstrated induction potential of phytoalexin gliceolin. In the third study It was evaluated the soybean seed health treated with potassium phosphites.. it was observed that the phosphites reduced the incidence of many fungi on seeds, especially of storage fungi like Aspergillus sp. and Fusarium semitectum. In the fourth study it was evaluated the in vitro effect of potassium phosphites on pathogenic fungi of the culture. And it was found direct action of phosphites on the mycelial growth of Fusarium semitectum, Pythium sp. and Sclerotinia sclerotiorum. Based on these results, we concluded that potassium phosphites have potential in seeds treatment, as resistance inducer and on in vitro control of phytopathogens.

Desempenho de sementes de soja revestidas com biopolímeros

The use of biopolymers that help to fix pesticides efficiently and degrade easily without harming the environment, and still improve the physiological performance of field soybean seed may bring contributions to the soybean yield. This study aimed to evaluate the effect of cassava starch polymers (AM), sodium alginate (ALG) and polyvinyl alcohol (PVOH), in the concentrations 2, 4 and 6 g / 100 ml of solution, in the physiological attributes of seeds soy, seed speed soaking and performance of soybean seeds after three months of storage. The soybean variety used was the NK 7059 RR. The experimental design used for the three studies was a factorial with 48 experimental units: 3 polymers (AM, ALG and PVOH), 4 different concentrations (0%, 2%, 4% and 6%), with four replications, in a completely randomized design. It was observed the level of significance of the factors and their interactions, applying the test F. The polymers were evaluated by the Tukey test at 5% probability, and the concentrations were evaluated by polynomial regression. The witness obtained better results for most variables studied. Among the polymers, the best coating was observed PVOH because it was the less viscous polymer and visually not served as a substrate for microorganisms. However, also, satisfactory results were obtained for the AM and ALG polymers at a concentration of 2%. There was not interference of the polymers studied with regard to reduction of imbibition rate of soybean seeds. The hydrophilicity of polymers, mainly the AM and ALG accelerated soaking seeds harming germination at concentrations 4% and 6%. In general, the higher the concentration of polymers tended to worse results.

Efeito da luz, alagamento e salinidade na germinação e crescimento de sementes de Sesbania punicea (Cav.) Benth. (Fabaceae, Papilionoideae)

Sesbania punicea (Cav.) Benth. é um arbusto que pode atingir de 1-4m de altura, cuja floração ocorre na primavera e no verão, frutificando no outono. O fruto é do tipo legume reto, produzindo em média 10 sementes por fruto. Sua propagação é frequentemente facilitada por perturbações na natureza, sendo bastante tolerante a solos pobres se adaptando a qualquer clima. O presente estudo teve por objetivo verificar os requisitos para germinação de Sesbania punicea sob influência de fatores abióticos. As sementes de Sesbania utilizadas neste estudo foram coletadas na APA da Lagoa Verde, Rio Grande- RS. Para avaliar o investimento reprodutivo da espécie foram realizadas as medidas morfométricas de 200 sementes. Para o teste de germinação, foram utilizados dois tipos de tratamentos: umidade e alagamento, ambos nas condições de luz e escuro, à temperatura de 25ºC, submetidos a quatro potenciais salinos: 0, 5, 10 e 15g de NaClL -1 . No teste em alagamento foram colocadas 100 sementes para cada salinidade em tubos Falcon transparentes, e para umidade, o mesmo número de sementes foi disposta em caixas gerbox transparentes contendo papel germitest, distribuídas de acordo com as respectivas salinidades. Os testes tiveram duração de 30 dias e a germinação foi acompanhada diariamente. Para avaliar o crescimento, 80 sementes foram colocadas em copos de poliestireno transparente de 100 ml, sendo 10 sementes para cada teste e, para análise da concentração de pigmentos, foram utilizadas plântulas com 30 dias de idade, crescendo nas mesmas condições de salinidade em que as sementes germinaram. As amostras de plântulas foram pesadas e logo após maceradas com 100 ml de acetona a 80% de concentração. A seguir, foram realizadas as leituras da concentração dos pigmentos em espectrofotômetro. As sementes de S. punicea utilizadas neste estudo, apresentaram tamanho pequeno (0,6 ± 0,01 cm). Houve respostas positivas de germinação tanto em presença quanto em ausência de luz, embora a germinação no teste em luz tenha sido maior, assim como a germinação no tratamento Alagado/ Luz, em 0g NaCl L-1 . Ao serem realizados os testes adicionando a variável salinidade, as respostas de germinação destas sementes mostraram um comportamento diferenciado, tendo um número menor de sementes germinadas com o aumento da concentração salina. As plântulas cresceram mais nos tratamentos em alagado e o aumento da concentração salina também reduziu o tamanho e o número de folhas das mesmas. No experimento de extração de pigmentos, clorofila a e b, os testes não demonstraram diferenças entre os tratamentos. Embora sementes de Sesbania punicea germinem sob influência de salinidade, o baixo desenvolvimento das plântulas observadas neste estudo sinalizam o cuidado que deve ser tomado ao utilizarmos a espécie para recuperação de áreas degradadas em ambientes com influência de salinidade.

Desempenho de soja associado à posição do fertilizante e tamanho de sementes

The seed size used for seeding has caused doubts among soybean producers. The study aimed to determine whether there may be differences between seed size with respect to depth of fertilizer deposition. The field experiment was conducted at the Experimental Area UTFPR Campus Pato Branco, using a precision seeder for direct seeding. The design was a randomized blocks, with five repetitions. The treatments were composed by the combination of two seed sizes (large seed with 6,5 mm and 5,5 mm with small seed) and two fertilizer deposition depths in relation to the seed (fertilizer near the seed with about 3 cm away and fertilizer distant from the seeds with about 10 cm). Data were subjected to analysis of variance. When the test value F was significant at 5% probability was applied to the Duncan test for comparison of means. The shallower depth of fertilizer deposition provided larger number of pods per plant and increased number of grains per plant. Already the largest depth of fertilizer deposition provided greater plant height at 30 days after sowing and R2 stage, greater ground area mobilized, higher plant population in all periods, greater depth of deposition of seeds and a higher rate of emergency speed.

Perfil de ácidos gordos de sementes: benefícios para a saúde

Introdução e Objetivos: Nos últimos anos tem-se verificado um aumento na procura de sementes oleaginosas, sobretudo de girassol, sésamo, linhaça e pevides de abóbora, porque o seu consumo está associado a efeitos benéficos para a saúde. Recentemente, surgiram no mercado "novas" sementes que agora fazem parte da nossa alimentação, como por exemplo, as sementes de chia e de papoila. Normalmente, este tipo de produtos são adicionados a outros alimentos como batidos, iogurtes, sumos de fruta, ou são usados como ingredientes na produção de produtos de padaria e/ou pastelaria. O objetivo deste trabalho foi determinar o perfil de ácidos gordos de diferentes tipos de sementes que estão frequentemente disponíveis no mercado Português. Material e Métodos: Foram adquiridas em 2015, nas superfícies comerciais e ervanárias da região de Lisboa, oito tipos de sementes. Determinou-se o perfil de ácidos gordos por cromatografia gasosa das seguintes amostras: Papoila (Papaver somniferum L.), chia (Salvia hispanica), alpista (Phalaris canariensis L.), cânhamo (Cannabis sativa L.), abóbora (Cucurbita L.), girassol (Helianthus annuus L.), sésamo (Sesamum indicum L.) e linhaça (Linum usitatissimum L.). Resultados e Discussão: Verificou-se que para as sementes de linhaça e chia o principal ácido gordo foi o ácido alfa-linolénico (C18:3, n3), com valores que variaram entre 45,9% e 64,4% do total de ácidos gordos, para sementes de linhaça e sementes de chia, respetivamente. Para as restantes amostras o principal ácido gordo foi o ácido linoleico (C18:2, n6). As sementes de papoila apresentaram o maior teor de ácido linoleico (71,6% do total de ácidos gordos), e as sementes de sésamo apresentaram o teor mais elevado de ácido oleico (39,6% do total de ácidos gordos). Conclusões: Todas as sementes analisadas apresentam um perfil de ácidos gordos saudável, sendo estes sobretudo ácidos gordos insaturados relacionados com efeitos benéficos na prevenção de doenças cardiovasculares. Este estudo fornece novos dados sobre o perfil de ácidos gordos de sementes oleaginosas amplamente disponíveis, que poderão ser úteis para avaliar o padrão alimentar da população Portuguesa, mas também para o desenvolvimento de futuras recomendações e orientações alimentares.