999 resultados para pH concentration
Resumo:
Isoelectric focusing of human urinary metallothionein at a pH range of 4.8 to 7.0 yielded a single protein band with a pI of 5.57 which co-migrated with authentic purified metallothionein I from human liver. Minimum pretreatment of the urine samples (160 ml) was needed. The preparatory steps included sample concentration with the original protein, enriched from 69 +/- 23 micrograms/ml to 2.0 +/- 1.4 mg/ml (+/- SD; n = 9), followed by heat treatment at 80 degrees C for 5 min (2.4 +/- 1.7 mg protein/ml). After focusing, the gels were stained with silver and the lanes were scanned with a laser scanner. Peak areas were used for quantitation with commercial beta 2-microglobulin as a standard. The urinary metallothionein ranged from 1.0 to 2.6 nmol/mmol creatinine, which is comparable with values reached by radio-immunoassay.
Resumo:
1858 (T5)-1859.
Resumo:
1851 (T1)-1852.
Resumo:
1857 (T5)-1858.
Resumo:
1855 (T4)-1856.
Resumo:
1897 (TABLE = 1851).
Resumo:
1851 (T1)-1853.
Resumo:
1859 (T6)-1860.
Resumo:
1857 (T4)-1858.
Resumo:
1861 (SER2,T1).
Resumo:
1853 (T2)-1854.
Resumo:
1862 (SER2,T2).
Resumo:
1853 (T3)-1855.
Resumo:
1852 (T2)-1853.
