Fipronil na bioenergética de mitocôndrias isoladas de figado de rato

Pós-graduação em Ciência Animal - FMVA

Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1 M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1 M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.

Caracterização histoquímica e imuno-histoquímica das alterações fibróticas nas endometroses das éguas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Migração e invasão celular in vitro de células humanas expressando variantes da oncoproteína LMP1 do vírus de Epstein-Barr (EBV)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Terapia gênica com interferon-alfa no controle do câncer colorretal

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Transporte mucociliar e função autonômica de tabagistas submetidos ao esforço físico submáximo e máximo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Funcionalidade das proteínas EF-Tu e 14-3-3 na virulência de Paracoccidioides brasiliensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos do treinamento físico sobre o estresse oxidativo em fígado e pâncreas de ratos diabéticos

Pós-graduação em Ciências da Motricidade - IBRC

Recuperação de biomassa bacteriana produzida no tratamento de efluente industrial

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Regeneração óssea guiada com o uso de matriz óssea bovina desmineralizada em defeitos ósseos experimentais em cães

Background: The principles of tissue regeneration to repair alveolar bone defects are based on the fabrication of a biologic barrier with different biomaterials. Therefore, the present study aimed to investigate the guided bone regeneration (GBR) by using membrane of demineralized bovine bone matrix (DBBM) on experimental defects in tibia of dogs. Methods: Four dogs were used and after anesthesia, shaving and antisepsis, two standardized bone defects were created on the right tibia of each animal with trephine drill. In the Control Group, the defects were filled with blood coagulum, while in the Treated Group, a membrane of DBBM was used to cover the defects. After 90 days, animals were sacrificed. Results: In the Treated Group, 67.4% of new bone formation was observed and, in the Control Group, 32.6% of the bone tissue reabsorbed when compared with initial bone volume. The membrane remained intact and no inflammatory tissue was observed on membrane/ bone interface. Conclusion: It was concluded that the use of DBBM is an osteoconductive material, presents biocompatibility and may be promise option to repair bone defects.

Regeneração óssea guiada em implantodontia: relato de caso

Objective: The use of methods for tissue regeneration has been widely applied in Implantology, in clinical situations with disabilities or anatomical limitations that prevent the placement of osseointegrated dental implants. The evolution of the development of biomaterials revolutionized this therapeutic modality, facilitating the resolution of clinical cases with tissue deficiencies. Thus, this study aimed to describe a clinical case approaching the methods, techniques, and materials used in guided bone regeneration applied to Implantology. Case report: A clinical case of a patient who received a Morse taper dental implant (region 15) is described. The use of biomaterial and membrane on the buccal wall of the socket was required. After the osseointegration period, a reopening surgery was performed, and an immediate provisional implant was produced. After 2 months of follow-up, the final prosthesis was made involving other adjacent elements. Final considerations: The guided bone regeneration technique employed showed satisfactory performance. The patient was positive regarding esthetics and function. However, more controlled studies with longer follow-up period are needed for analyses of predictability

Root reconstructed with mineral trioxide aggregate and guided tissue regeneration in apical surgery: a 5-year follow-up

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Osteotomia ventral da bula timpânica em gatos: revisão de literatura

Otite média e pólipos são as principais afecções da orelha média em gatos. A otite média geralmente é decorrente de otite externa crônica, enquanto os pólipos se formam a partir de um processo inflamatório crônico presente na orelha média. Quando o pólipo sai da cavidade timpânica pela tuba auditiva, aparecendo na nasofaringe, é denominado pólipo nasofaríngeo. Outra possibilidade é o pólipo romper a membrana do tímpano e surgir no meato acústico externo. O tratamento indicado para essas afecções é a osteotomia ventral da bula timpânica, procedimento cirúrgico cuja finalidade é remover exsudatos inflamatórios presentes na cavidade timpânica. Apesar de possíveis complicações, como síndrome de Horner e lesão na orelha interna, essa cirurgia proporciona resultados muito satisfatórios. O objetivo da presente revisão é relatar essas afecções da orelha média em gatos e descrever a técnica cirúrgica de osteotomia ventral da bula timpânica.

Determinação in situ de metilmercúrio e mercúrio (II) em sistemas aquáticos nas proximidades de refinarias de petróleo, utilizando a técnica difusão em filmes finos por gradiente de concentração (DGT)

Pós-graduação em Geociências e Meio Ambiente - IGCE

Análise da qualidade microbiológica do peixe (Eugerres brasilianus, Curvier 1830) e das águas do Estuário do Rio Itanhaém, SP, Brasil

Pós-graduação em Ciências Biológicas (Microbiologia Aplicada) - IBRC